GSE124756 Comparison of Transcriptional Signatures of Three Staphylococcal Superantigenic Toxins in Human Epidermal Melanocytes

Contributors : Seshamalini Srinivasan ; Nabarun Chakraborty ; Ruoting Yang ; Stacy Ann-Miller ; Aarti Gautam ; Abdulnaser Alkhalil ; Lauren T Moffatt ; Bonnie C Carney ; Marti Jett ; Jeffrey W Shupp ; Rasha Hammamieh ; Marti JettSeries Type : Expression profiling by arrayOrganism : Homo sapiensStaphylococcus aureus, a gram-positive bacterium, causes food poisoning and toxic shock syndrome through the production of superantigenic toxins known as Staphylococcal enterotoxins serotypes A-J (SEA, SEB, etc.) and toxic shock syndrome toxin-1 (TSST-1). A subset of these toxins have been classified as potential biothreat agents. The chronology of molecular events that could potentially characterize superantigenic toxicity and early pathogenesis is not well understood. The focus of this study was to determine the distinct and shared mechanisms of response to three toxins of the superantigenic family, namely SEA, SEB and TSST-1. Since skin functions as the front line of the host ’s defense mechanism, melanocytes were selected for the study and treated with 25 µg/mL of one of these three toxins. Cells were collected after treatment for six different time periods, ranging from 0.5 h to 48 h. Total RNA was investigated using gene expression microarrays containing approximat ely 50,000 probes, and a subset of the results were validated using NanoString assays. Transcriptomic expression data indicated that each of these three toxins had a unique longitudinal trajectory. In parti...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research