The use of the internal transcribed spacer region for phylogenetic analysis of the microsporidian parasite Enterocytozoon hepatopenaei infecting whiteleg shrimp (Penaeus vannamei) and for the development of a nested PCR as its diagnostic tool
In this study, the ITS-1 region sequence (~600-bp) of EHP was first identified, and primers for a polymerase chain reaction (PCR) assay targeting that sequence were designed. A newly developed nested-PCR method successfully detected the EHP in various shrimp (Penaeus vannamei and P. monodon) and related samples, including water and feces collected from Indonesia, Thailand, South Korea, India, and Malaysia. The primers did not cross-react with other hosts and pathogens, and this PCR assay is more sensitive than existing PCR detection methods targeting the small subunit ribosomal RNA (SSU rRNA) and spore wall protein (SWP) g...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Ju Hee Lee Hye Jin Jeon Sangsu Seo Chorong Lee Bumkeun Kim Dong-Mi Kwak Man Hee Rhee Patharapol Piamsomboon Yani Lestari Nuraini Chang Uook Je Seon Young Park Ji Hyung Kim Jee Eun Han Source Type: research
The use of the internal transcribed spacer region for phylogenetic analysis of the microsporidian parasite Enterocytozoon hepatopenaei infecting whiteleg shrimp (Penaeus vannamei) and for the development of a nested PCR as its diagnostic tool
In this study, the ITS-1 region sequence (~600-bp) of EHP was first identified, and primers for a polymerase chain reaction (PCR) assay targeting that sequence were designed. A newly developed nested-PCR method successfully detected the EHP in various shrimp (Penaeus vannamei and P. monodon) and related samples, including water and feces collected from Indonesia, Thailand, South Korea, India, and Malaysia. The primers did not cross-react with other hosts and pathogens, and this PCR assay is more sensitive than existing PCR detection methods targeting the small subunit ribosomal RNA (SSU rRNA) and spore wall protein (SWP) g...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Ju Hee Lee Hye Jin Jeon Sangsu Seo Chorong Lee Bumkeun Kim Dong-Mi Kwak Man Hee Rhee Patharapol Piamsomboon Yani Lestari Nuraini Chang Uook Je Seon Young Park Ji Hyung Kim Jee Eun Han Source Type: research
The use of the internal transcribed spacer region for phylogenetic analysis of the microsporidian parasite Enterocytozoon hepatopenaei infecting whiteleg shrimp (Penaeus vannamei) and for the development of a nested PCR as its diagnostic tool
In this study, the ITS-1 region sequence (~600-bp) of EHP was first identified, and primers for a polymerase chain reaction (PCR) assay targeting that sequence were designed. A newly developed nested-PCR method successfully detected the EHP in various shrimp (Penaeus vannamei and P. monodon) and related samples, including water and feces collected from Indonesia, Thailand, South Korea, India, and Malaysia. The primers did not cross-react with other hosts and pathogens, and this PCR assay is more sensitive than existing PCR detection methods targeting the small subunit ribosomal RNA (SSU rRNA) and spore wall protein (SWP) g...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Ju Hee Lee Hye Jin Jeon Sangsu Seo Chorong Lee Bumkeun Kim Dong-Mi Kwak Man Hee Rhee Patharapol Piamsomboon Yani Lestari Nuraini Chang Uook Je Seon Young Park Ji Hyung Kim Jee Eun Han Source Type: research
The use of the internal transcribed spacer region for phylogenetic analysis of the microsporidian parasite Enterocytozoon hepatopenaei infecting whiteleg shrimp (Penaeus vannamei) and for the development of a nested PCR as its diagnostic tool
In this study, the ITS-1 region sequence (~600-bp) of EHP was first identified, and primers for a polymerase chain reaction (PCR) assay targeting that sequence were designed. A newly developed nested-PCR method successfully detected the EHP in various shrimp (Penaeus vannamei and P. monodon) and related samples, including water and feces collected from Indonesia, Thailand, South Korea, India, and Malaysia. The primers did not cross-react with other hosts and pathogens, and this PCR assay is more sensitive than existing PCR detection methods targeting the small subunit ribosomal RNA (SSU rRNA) and spore wall protein (SWP) g...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Ju Hee Lee Hye Jin Jeon Sangsu Seo Chorong Lee Bumkeun Kim Dong-Mi Kwak Man Hee Rhee Patharapol Piamsomboon Yani Lestari Nuraini Chang Uook Je Seon Young Park Ji Hyung Kim Jee Eun Han Source Type: research
The use of the internal transcribed spacer region for phylogenetic analysis of the microsporidian parasite Enterocytozoon hepatopenaei infecting whiteleg shrimp (Penaeus vannamei) and for the development of a nested PCR as its diagnostic tool
In this study, the ITS-1 region sequence (~600-bp) of EHP was first identified, and primers for a polymerase chain reaction (PCR) assay targeting that sequence were designed. A newly developed nested-PCR method successfully detected the EHP in various shrimp (Penaeus vannamei and P. monodon) and related samples, including water and feces collected from Indonesia, Thailand, South Korea, India, and Malaysia. The primers did not cross-react with other hosts and pathogens, and this PCR assay is more sensitive than existing PCR detection methods targeting the small subunit ribosomal RNA (SSU rRNA) and spore wall protein (SWP) g...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Ju Hee Lee Hye Jin Jeon Sangsu Seo Chorong Lee Bumkeun Kim Dong-Mi Kwak Man Hee Rhee Patharapol Piamsomboon Yani Lestari Nuraini Chang Uook Je Seon Young Park Ji Hyung Kim Jee Eun Han Source Type: research
The use of the internal transcribed spacer region for phylogenetic analysis of the microsporidian parasite Enterocytozoon hepatopenaei infecting whiteleg shrimp (Penaeus vannamei) and for the development of a nested PCR as its diagnostic tool
In this study, the ITS-1 region sequence (~600-bp) of EHP was first identified, and primers for a polymerase chain reaction (PCR) assay targeting that sequence were designed. A newly developed nested-PCR method successfully detected the EHP in various shrimp (Penaeus vannamei and P. monodon) and related samples, including water and feces collected from Indonesia, Thailand, South Korea, India, and Malaysia. The primers did not cross-react with other hosts and pathogens, and this PCR assay is more sensitive than existing PCR detection methods targeting the small subunit ribosomal RNA (SSU rRNA) and spore wall protein (SWP) g...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Ju Hee Lee Hye Jin Jeon Sangsu Seo Chorong Lee Bumkeun Kim Dong-Mi Kwak Man Hee Rhee Patharapol Piamsomboon Yani Lestari Nuraini Chang Uook Je Seon Young Park Ji Hyung Kim Jee Eun Han Source Type: research
The use of the internal transcribed spacer region for phylogenetic analysis of the microsporidian parasite Enterocytozoon hepatopenaei infecting whiteleg shrimp (Penaeus vannamei) and for the development of a nested PCR as its diagnostic tool
In this study, the ITS-1 region sequence (~600-bp) of EHP was first identified, and primers for a polymerase chain reaction (PCR) assay targeting that sequence were designed. A newly developed nested-PCR method successfully detected the EHP in various shrimp (Penaeus vannamei and P. monodon) and related samples, including water and feces collected from Indonesia, Thailand, South Korea, India, and Malaysia. The primers did not cross-react with other hosts and pathogens, and this PCR assay is more sensitive than existing PCR detection methods targeting the small subunit ribosomal RNA (SSU rRNA) and spore wall protein (SWP) g...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Ju Hee Lee Hye Jin Jeon Sangsu Seo Chorong Lee Bumkeun Kim Dong-Mi Kwak Man Hee Rhee Patharapol Piamsomboon Yani Lestari Nuraini Chang Uook Je Seon Young Park Ji Hyung Kim Jee Eun Han Source Type: research
Classifying safe following distance for motorcycles to prevent rear-end collisions
This study aims to classify motorcycle (MC) following distance based on trajectory traffic data and identify the risks associated with MC following distances to prevent rear-end collisions. A total of 8,223 events of a MC following a vehicle were investigated in Pathum Thani, Thailand, and 41 cases of MC rear-end crashes were analyzed between 2017 and 2021. Time headway (TH), safe stopping distance (SSD) and time to collision (TTC) were applied to the proposed concept to determine safe following distance (SFD). Speed and following distance for actual rear-end crashes were applied to validate SFD. Results showed that the pr...
Source: International Journal of Injury Control and Safety Promotion - April 1, 2024 Category: Accident Prevention Authors: Phanuphong Prajongkha Kunnawee Kanitpong Source Type: research
Influencing Factors on Death Acceptance Among Thai Older Persons
CONCLUSIONS: This study highlights the significant direct and indirect effects on death acceptance among older Thai Buddhists.CLINICAL IMPLICATIONS: Buddhist-aligned interventions may be more effective and readily embraced by Thai older persons. For those who are receptive, nurses can foster death acceptance by developing interventions that reduce death anxiety and promote Buddhist beliefs about death, Buddhist practices, and social support.PMID:38557283 | DOI:10.1080/07317115.2024.2337118 (Source: Clinical Gerontologist)
Source: Clinical Gerontologist - April 1, 2024 Category: Geriatrics Authors: Kanoklekha Suwannapong Sureeporn Thanasilp Noppamat Pudtong Lanchasak Akkayagorn Sudaporn Stithyudhakarn Wilailuck Tantitrakul Dawn Doutrich Ratchaneekorn Upasen Source Type: research
Classifying safe following distance for motorcycles to prevent rear-end collisions
This study aims to classify motorcycle (MC) following distance based on trajectory traffic data and identify the risks associated with MC following distances to prevent rear-end collisions. A total of 8,223 events of a MC following a vehicle were investigated in Pathum Thani, Thailand, and 41 cases of MC rear-end crashes were analyzed between 2017 and 2021. Time headway (TH), safe stopping distance (SSD) and time to collision (TTC) were applied to the proposed concept to determine safe following distance (SFD). Speed and following distance for actual rear-end crashes were applied to validate SFD. Results showed that the pr...
Source: International Journal of Injury Control and Safety Promotion - April 1, 2024 Category: Accident Prevention Authors: Phanuphong Prajongkha Kunnawee Kanitpong Source Type: research
Influencing Factors on Death Acceptance Among Thai Older Persons
CONCLUSIONS: This study highlights the significant direct and indirect effects on death acceptance among older Thai Buddhists.CLINICAL IMPLICATIONS: Buddhist-aligned interventions may be more effective and readily embraced by Thai older persons. For those who are receptive, nurses can foster death acceptance by developing interventions that reduce death anxiety and promote Buddhist beliefs about death, Buddhist practices, and social support.PMID:38557283 | DOI:10.1080/07317115.2024.2337118 (Source: Clinical Gerontologist)
Source: Clinical Gerontologist - April 1, 2024 Category: Geriatrics Authors: Kanoklekha Suwannapong Sureeporn Thanasilp Noppamat Pudtong Lanchasak Akkayagorn Sudaporn Stithyudhakarn Wilailuck Tantitrakul Dawn Doutrich Ratchaneekorn Upasen Source Type: research
Stakeholder-Informed Solutions To Address Barriers for Alcohol Screening and Brief Intervention in Thai Hypertension Care
This study sought to identify key factors in successfully implementing alcohol use screening and brief intervention in hypertension care in Thailand. For this purpose, we surveyed participants (NRound 1 = 91, NRound 2 = 27) from three different groups of Thai stakeholders (policy- and decisionmakers, primary healthcare practitioners, and patients diagnosed with hypertension) in a two-round stakeholder elicitation. In round 1, we identified limited resources, lack of clear guidelines for lifestyle intervention, stigmatization, and inconsistent monitoring of patients ’ alcohol use as important barriers. In round 2, we soug...
Source: The Journal of Primary Prevention - April 1, 2024 Category: Sleep Medicine Source Type: research
Performance comparison between two computer-aided detection colonoscopy models by trainees using different false positive thresholds: a cross-sectional study in Thailand
CONCLUSION: Compared to CADe, Deep-GI demonstrated a higher PDS with significantly lower FPR at ≥0.5- and ≥1-second thresholds. At the ≥1.5-second threshold, both systems showed comparable FPR with increased PMR.PMID:38556473 | DOI:10.5946/ce.2023.145 (Source: Clinical Endoscopy)
Source: Clinical Endoscopy - March 31, 2024 Category: Gastroenterology Authors: Kasenee Tiankanon Julalak Karuehardsuwan Satimai Aniwan Parit Mekaroonkamol Panukorn Sunthornwechapong Huttakan Navadurong Kittithat Tantitanawat Krittaya Mekritthikrai Salin Samutrangsi Peerapon Vateekul Rungsun Rerknimitr Source Type: research
