Conformationally locked sugar derivatives and analogues as potential neuraminidase inhibitors
Eur J Med Chem. 2023 Apr 24;255:115410. doi: 10.1016/j.ejmech.2023.115410. Online ahead of print.ABSTRACTThe influenza virus remains a major health concern for mankind because it tends to mutate frequently and cause high morbidity. Influenza prevention and treatment are greatly aided by the use of antivirals. One such class of antivirals is neuraminidase inhibitors (NAIs), effective against influenza viruses. A neuraminidase on the virus's surface serves a vital function in viral propogation by assisting in the release of viruses from infected host cells. Neuraminidase inhibitors are the backbone in stoping such virus prop...
Source: European Journal of Medicinal Chemistry - April 30, 2023 Category: Chemistry Authors: Yogesh Yadav Rajdeep Tyagi Ramesh Kumar Ram Sagar Source Type: research
Conformationally locked sugar derivatives and analogues as potential neuraminidase inhibitors
Eur J Med Chem. 2023 Apr 24;255:115410. doi: 10.1016/j.ejmech.2023.115410. Online ahead of print.ABSTRACTThe influenza virus remains a major health concern for mankind because it tends to mutate frequently and cause high morbidity. Influenza prevention and treatment are greatly aided by the use of antivirals. One such class of antivirals is neuraminidase inhibitors (NAIs), effective against influenza viruses. A neuraminidase on the virus's surface serves a vital function in viral propogation by assisting in the release of viruses from infected host cells. Neuraminidase inhibitors are the backbone in stoping such virus prop...
Source: European Journal of Medicinal Chemistry - April 30, 2023 Category: Chemistry Authors: Yogesh Yadav Rajdeep Tyagi Ramesh Kumar Ram Sagar Source Type: research
Conformationally locked sugar derivatives and analogues as potential neuraminidase inhibitors
Eur J Med Chem. 2023 Apr 24;255:115410. doi: 10.1016/j.ejmech.2023.115410. Online ahead of print.ABSTRACTThe influenza virus remains a major health concern for mankind because it tends to mutate frequently and cause high morbidity. Influenza prevention and treatment are greatly aided by the use of antivirals. One such class of antivirals is neuraminidase inhibitors (NAIs), effective against influenza viruses. A neuraminidase on the virus's surface serves a vital function in viral propogation by assisting in the release of viruses from infected host cells. Neuraminidase inhibitors are the backbone in stoping such virus prop...
Source: European Journal of Medicinal Chemistry - April 30, 2023 Category: Chemistry Authors: Yogesh Yadav Rajdeep Tyagi Ramesh Kumar Ram Sagar Source Type: research
Viruses, Vol. 15, Pages 1056: Targeting the Human Influenza a Virus: The Methods, Limitations, and Pitfalls of Virtual Screening for Drug-like Candidates Including Scaffold Hopping and Compound Profiling
In this study, we describe the input data and processing steps to find antiviral lead compounds by a virtual screen. Two-dimensional and three-dimensional filters were designed based on the X-ray crystallographic structures of viral neuraminidase co-crystallized with substrate sialic acid, substrate-like DANA, and four inhibitors (oseltamivir, zanamivir, laninamivir, and peramivir). As a result, ligand–receptor interactions were modeled, and those necessary for binding were utilized as screen filters. Prospective virtual screening (VS) was carried out in a virtual chemical library of over half a million small...
Source: Viruses - April 26, 2023 Category: Virology Authors: Thomas Scior Karina Cuanalo-Contreras Angel A. Islas Ygnacio Martinez-Laguna Tags: Article Source Type: research
Viruses, Vol. 15, Pages 983: Detecting the Neuraminidase R294K Mutation in Avian Influenza A (H7N9) Virus Using Reverse Transcription Droplet Digital PCR Method
This study aimed to develop an RT-dd PCR method for detecting the R294K mutation in H7N9. Primers and dual probes were designed using the H7N9 NA gene and the annealing temperature was optimized at 58.0 °C. The sensitivity of our RT-dd PCR method was not significantly different from that of RT-qPCR (p = 0.625), but it could specifically detect R294 and 294K in H7N9. Among 89 clinical samples, 2 showed the R294K mutation. These two strains were evaluated using a neuraminidase inhibition test, which revealed that their sensitivity to oseltamivir was greatly reduced. The sensitivity and specificity of RT-dd PCR we...
Source: Viruses - April 17, 2023 Category: Virology Authors: Xiuyu Lou Hao Yan Lingxuan Su Yi Sun Xinyin Wang Liming Gong Yin Chen Zhen Li Zhongbiao Fang Haiyan Mao Keda Chen Yanjun Zhang Tags: Article Source Type: research
Clinical misdiagnosis of influenza infection with a confusing clinical course: A case report
AbstractA 32-year-old woman with a history of hypothyroidism and major depressive disorder was admitted with severe weakness and somnolence. She had tachycardia and hypotension, indicative of severe dehydration, and was treated with a vasopressor and sodium bicarbonate, but her clinical manifestations deteriorated. A high-resolution computed tomography (HRCT) scan showed a patchy ground glass appearance with interlobular septal thickening, suggesting pneumonia. Reverse transcription-polymerase chain reaction (RT-PCR) was requested for the influenza A virus (IAV), which was positive. The patient was treated with oseltamivir...
Source: Clinical Case Reports - April 13, 2023 Category: General Medicine Authors: Arefeh Babazadeh,
Zeinab Mohseni Afshar,
Mohammad Barary,
Rezvan Hosseinzadeh,
Soheil Ebrahimpour Tags: CASE REPORT Source Type: research
H3N2 influenza hemagglutination inhibition method qualification with data driven statistical methods for human clinical trials
We report on a qualified and adaptable influenza serology method and analysis strategy to measure quantifiable HAI titers to define correlates of vaccine mediated protection in human clinical trials. (Source: Frontiers in Immunology)
Source: Frontiers in Immunology - April 6, 2023 Category: Allergy & Immunology Source Type: research
Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants
In this study, based on an existing reverse transcriptase real-time PCR (RT-qPCR) assay, we developed a reverse transcriptase droplet digital PCR assay (RT-ddPCR) to detect and quantify the frequency of the E119V-NA mutation. Furthermore, reverse genetics viruses carrying this mutation were created to test the performance of the RT-ddPCR assay and compare it to the standard phenotypic NA assay. We also discuss the advantage of using an RT-ddPCR instead of qPCR method in the context of viral diagnostics and surveillance.PMID:36975535 | PMC:PMC10047791 | DOI:10.3390/cimb45030165 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - March 28, 2023 Category: Molecular Biology Authors: Laura A E Van Poelvoorde Fran çois E Dufrasne Steven Van Gucht Xavier Saelens Nancy H C Roosens Source Type: research
