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Argon preconditioning protects neuronal cells with a Toll-like receptor-mediated effect
In this study, human neuroblastoma SH-SY5Y cells were treated with different concentrations of argon (25%, 50%, and 74%; 21% O2, 5% CO2, balance nitrogen) at different time intervals before inflicting damage with rotenone (20 µM, 4 hours). Apoptosis was determined by flow cytometry after annexin V and propidium iodide staining. Surface expressions of Toll-like receptors 2 and 4 were also examined. Cells were also processed for analysis by western blot and qPCR to determine the expression of apoptotic and inflammatory proteins, such as extracellular-signal regulated kinase (ERK1/2), nuclear transcription factor-κB (NF-κB...
Source: Cell Research - December 1, 2022 Category: Cytology Authors: Stefanie Scheid Adrien Lejarre Jakob Wollborn Hartmut Buerkle Ulrich Goebel Felix Ulbrich Source Type: research

Orexin-A protects against oxygen-glucose deprivation/reoxygenation-induced cell damage by inhibiting endoplasmic reticulum stress-mediated apoptosis via the Gi and PI3K signaling pathways.
Abstract The neuropeptide orexin-A (OXA) has a neuroprotective effect, acting as an anti-apoptotic factor in response to multiple stimuli. Apoptosis induced by endoplasmic reticulum stress (ERS) underlies oxygen-glucose deprivation and reoxygenation (OGD/R)-induced cell damage, an in vitro model of ischemia/reperfusion injury. However, that OXA inhibits ERS-induced apoptosis in the OGD/R model has not been reported. In the present study, we investigated the neuroprotective effect of OXA (0.1 μM) on OGD/R-induced damage in the human neuroblastoma cell line SH-SY5Y. After OXA treatment following 4 h oxygen-gluc...
Source: Cellular Signalling - June 20, 2019 Category: Cytology Authors: Kong T, Qiu K, Liu M, Cheng B, Pan Y, Yang C, Chen J, Wang C Tags: Cell Signal Source Type: research

Up-regulation of miR-122 protects against neuronal cell death in ischemic stroke through the heat shock protein 70-dependent NF- κB pathway by targeting FOXO3.
Up-regulation of miR-122 protects against neuronal cell death in ischemic stroke through the heat shock protein 70-dependent NF-κB pathway by targeting FOXO3. Exp Cell Res. 2018 Apr 28;: Authors: Guo D, Ma J, Li T, Yan L Abstract Dysfunction of the microRNA (miR) network has been emerging as a main regulator in ischemic stroke. Recently, studies have linked the deregulation of miR-122 to ischemic stroke. However, the specific role and molecular mechanism of miR-122 in ischemic stroke remain to be further investigated. Here, we found that miR-122 was decreased in mouse N2A neuroblastoma (N2A) cells af...
Source: Experimental Cell Research - April 28, 2018 Category: Cytology Authors: Guo D, Ma J, Li T, Yan L Tags: Exp Cell Res Source Type: research

MiR-29b expression is associated with a dexmedetomidine-mediated protective effect against oxygen-glucose deprivation-induced injury to SK-N-SH cells in vitro.
Abstract Ischemic cerebral stroke is a leading cause of death and long-term disability world-wide. Neuronal injury following cerebral ischemia initiates a complex series of signaling cascades that lead to neuronal cell death. MicroRNA 29b (miR-29b) has reported involvement in the pathogenic process of ischemic brain injury. Dexmedetomidine (Dex) is a highly selective α2 adrenergic receptor stimulant that exerts a protective effect on brain tissue. To determine whether Dex might directly influence miR-29b expression after an ischemic injury, human neuroblastoma SK-N-SH cells were subjected to oxygen-glucose depriv...
Source: Cell Biology International - October 31, 2017 Category: Cytology Authors: Huang Z, Liu G, Zeng Q, Gao R, Zhang S, Wang L, Liu B, Yu Y, Zhao A, Li R, Zhou S, Yu W Tags: Cell Biol Int Source Type: research

Protection of seven dibenzocyclooctadiene lignans from Schisandra chinensis against serum and glucose deprivation injury in SH-SY5Y cells.
This study was conducted to evaluate the effects of seven dibenzocyclooctadiene lignans of Schisandra chinensis and explore the possible mechanisms in the human neuroblastoma SH-SY5Y cells exposed on serum and glucose deprivation (SGD) injury. The structure-activity relationships were also analyzed. Cell viability and lactate dehydrogenase (LDH) release were determined to evaluate cell injury. Inflammation and apoptosis-related protein levels were detected to elucidate the possible mechanisms. Schisantherin A, schizandrin C and schizandrol B were found to have stronger protective effects than schizandrin A, schizandrin B a...
Source: Cell Biology International - August 20, 2015 Category: Cytology Authors: E Q, Tang M, Zhang X, Shi Y, Wang D, Gu Y, Li S, Liang X, Wang Z, Wang C Tags: Cell Biol Int Source Type: research

Genetic ablation and short-duration inhibition of lipoxygenase results in increased macroautophagy.
Abstract 12/15-lipoxygenase (12/15-LOX) is involved in organelle homeostasis by degrading mitochondria in maturing red blood cells and by eliminating excess peroxisomes in liver. Furthermore, 12/15-LOX contributes to diseases by exacerbating oxidative stress-related injury, notably in stroke. Nonetheless, it is unclear what the consequences are of abolishing 12/15-LOX activity. Mice in which the alox15 gene has been ablated do not show an obvious phenotype, and LOX enzyme inhibition is not overtly detrimental. We show here that liver histology is also unremarkable. However, electron microscopy demonstrated that 12...
Source: Experimental Cell Research - November 26, 2013 Category: Cytology Authors: Jang I, Park S, Won Cho J, Yigitkanli K, van Leyen K, Roth J Tags: Exp Cell Res Source Type: research