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Source: GEO: Gene Expression Omnibus

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Total 1028 results found since Jan 2013.

GSE217342 TSPAN18 facilitates bone metastasis of prostate cancer by protecting STIM1 from TRIM32-mediated ubiquitination
Contributors : Qianghua Zhou ; Tianxin Lin ; Hai HuangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe demonstrated that TSPAN18 was overexpressed in prostate cancer (PCa) and markedly accelerated PCa cell migration and invasion in vitro and bone metastasis in vivo by inhibiting E3 ligase tripartite motif containing 32 (TRIM32)-mediated stromal interaction molecule ubiquitination and degradation. To further investigate the genes regulated by TSPAN18, we knockdown TSPAN18 by siRNA and performed high-throughput sequence. So we identify a series of genes regulated by TSPAN18.
Source: GEO: Gene Expression Omnibus - January 1, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE216708 Long non-coding RNA associated with mitogen-activated protein kinase in pancreatic cancer cells.
Contributors : Tomohiko Ishikawa ; Toru FurukawaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo investigate the genes associated with LINC00941, we isolated total RNA from AsPC-1 with or without knockdown of LINC00941 using siRNA.Total transcriptome sequencing analysis was performed in total 3 samples.
Source: GEO: Gene Expression Omnibus - January 1, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE190615 The transcriptomic and proteomic map of human primary cells
Contributor : Pandey AkhileshSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensMolecular profiling of human primary cell types is essential for holistic understanding of human biology. Here we present a map of 28 human primary cell types with comprehensive measurements of expressed transcripts and proteins. Three major clusters of epithelial, endothelial, and mesenchymal cell types were observed both at transcriptome and proteome level along with discovery of several cell type specific proteins. Among them, we characterized the novel epithelial specific protein C1orf116 which was furthe...
Source: GEO: Gene Expression Omnibus - December 31, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE178987 An RNAi therapeutic targeting hepatic DGAT2 in a genetically obese mouse model of nonalcoholic steatohepatitis
Contributors : Batuhan Yenilmez ; Lawrence Lifshitz ; Michael Czech ; Anastasia KhvorovaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusNonalcoholic steatohepatitis (NASH) is a severe liver disorder characterized by triglyceride accumulation, severe inflammation, and fibrosis. With the recent increase in prevalence, NASH is now the leading cause of liver transplantation, with no approved therapeutics available. Despite years of research, the exact molecular mechanism of NASH progression is not well understood, but fat accumulation is believed to be the primary driver of the disease. T...
Source: GEO: Gene Expression Omnibus - December 31, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE207213 Effect of depletion of YTH family genes on gene expression in Nipponbera rices
Contributors : Weiwei Ma ; Xiaole WangSeries Type : Expression profiling by high throughput sequencingOrganism : Oryza sativaTo investigate the function YTH proteins in the regulation of gene expression, we established 12 transgenic lines in which each target gene has been knocked out by CRISPR/Cas9 or knocked down by siRNA.We then performed gene expression profiling analysis using data obtained from RNA-seq of 45 different transgenic lines.
Source: GEO: Gene Expression Omnibus - December 31, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Oryza sativa Source Type: research

GSE202053 Genome-wide distribution of chromatin accessibility and transcriptome of Set2-deficient Drosophila photoreceptor neurons
We report the chromatin accessibility landscape and transcriptome of Drosophila photoreceptor neurons that are Set2-deficient (via siRNA) obtained using a tissue-specific approach from 10-day old flies
Source: GEO: Gene Expression Omnibus - December 31, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Drosophila melanogaster Source Type: research

GSE85496 Microarray mRNA expression profiling in Stat5b knockdown pancreatic beta cell line
Contributors : Malini Laloraya ; Annu JosephSeries Type : Expression profiling by arrayOrganism : Mus musculusStat5b gene is located at IDD4 type 1 diabetes susceptibility interval, and is mutated in NOD mice. This mutation has been linked to diabetes with strong association with autoimmune aetiology. We have treated MIN6 pancreatic beta cell line with Stat5b siRNA and analyzed the differential expression profile in control siRNA treated versus Stat5b siRNA treated cells in an effort to understand the role of Stat5b in diabetes.
Source: GEO: Gene Expression Omnibus - December 31, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research

GSE86179 Knockdowns of total NFYA and a NFYA long 3 ’ UTR isoform followed by RNA-seq to determine isoform-specific effects on gene expression
Contributors : Thomas J Sweet ; Jeffrey M Bhasin ; Angela H TingSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensAlternative cleavage and polyadenylation (APA) produces multiple isoforms of the Nuclear Transcription Factor Y Subunit Alpha (NFYA) gene with variable length 3? untranslated regions (UTRs). These isoforms have been detected in HCT116 colon cancer cells via polyA-seq and northern blots, as well as colon cancer tissue RNA-seq data. NFYA is a transcription factor that may regulate cell growth and proliferation in cancer and development. To determine if there may be transcripti...
Source: GEO: Gene Expression Omnibus - December 28, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE192644 Studying heterogeneity in embryonic stem cells using the approach of search-engine
Contributors : Kedar N Natrajan ; Vibhor KumarSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusHere we designed a search-engine for single-cell epigenome profiles. We tested different application of search-engine using different data-sets including mESC scATAC-seq profile.
Source: GEO: Gene Expression Omnibus - December 27, 2022 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Mus musculus Source Type: research

GSE218853 The effect of background noise and its removal on the analysis of single-cell expression data
CONCLUSION: Our findings help to better understand the extent, sources and impact of background noise in single-cell experiments and provide guidance on how to deal with it.
Source: GEO: Gene Expression Omnibus - December 10, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE198484 RNA sequencing of HUVECs stimulated with VEGF, OSM+VEGF, or CNTF+CNTFR+VEGF with or without STAT3 knock-down
Contributors : Julian Rapp ; Felicitas BucherSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe role of STAT3 signaling induced by stimulation of IL-6 cytokine family members such as OSM and CNTF on the angiogenic response of endothelial cells is still not fully understood. To assess the impact of this pathway on angiogenesis, this study compares human umbilical vein endothelial cells (HUVECs) with or without STAT3 knock-down introduced by siRNA and stimulation with VEGF, OSM+VEGF and CNTF+VEGF in the presence of the CNTF receptor (CNTFR).
Source: GEO: Gene Expression Omnibus - December 4, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE140966 RNA-Seq profiling of circular RNAs and the oncogenic role of circPVT1 in cutaneous squamous cell carcinoma
Contributors : Shuang Chen ; Chundi HeSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCircular RNAs(circRNAs),which are a type of non-coding RNA with a special structure, have attracted the attention of researchers in recent years.As a high throughput gene screening method, RNA-seq has been widely used in tumor research.In present study,we assessed the expression pattern of circRNAs in CSCC via RNA-seq on CSCC and matched paracancerous samples. We further identified circPVT1 as being overexpressed in CSCC tissues and cells.Subsequently, we identified the characteristics of circPVT1 in...
Source: GEO: Gene Expression Omnibus - November 24, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE191323 Characterization of normal fibroblast transcriptomes upon transient silencing of LTBP2 by RNAseq
Conclusions: Our study explored LTBP2 functions in normal fibroblasts. RNAseq data support its important role. After 48h, LTBP2 silencing induces changes in gene expression of numerous genes involved in fibroblast functions.
Source: GEO: Gene Expression Omnibus - November 22, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE214881 Targeting Xkr8 via nanoparticle-mediated in situ codelivery of siRNA and chemotherapy drugs for cancer immunochemotherapy
Contributors : Yuang Chen ; Zhangyi Luo ; Runzi Sun ; Song LiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusDownregulation of specific proteins named scramblases might enhance tumour immunosuppression. In this paper the authors first show that the scramblase Xrk8 is overexpressed in tumour cells upon treatment with chemotherapeutics, and then developed a nanomedicine platform for co-delivery of a cancer pro-drug and of a siRNA directed against the Xrk8 gene, showing therapeutic effect and enhanced immune response in animal tumour models.
Source: GEO: Gene Expression Omnibus - November 18, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE197308 siRNAs targeting a chromatin-associated RNA induce its transcriptional silencing in human cells
Contributors : Julien Ouvrard ; Marion Aguirrebengoa ; Estelle Nicolas ; Didier TroucheSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTranscriptional gene silencing by small interfering RNAs (siRNAs) has been widely described in various species, such as plants or fission yeasts. In mammals, its extent remained somewhat debated. Previous studies showed that siRNAs targeting gene promoters can induce the silencing of the targeted promoter, although the involvement of off-target mechanisms was also suggested. Here, by nascent RNA capture and RNA Pol II ChIP, we show that siRNAs targetin...
Source: GEO: Gene Expression Omnibus - November 18, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research