Filtered By:
Specialty: Biotechnology
Therapy: Gene Therapy

This page shows you your search results in order of date.

Order by Relevance | Date

Total 22 results found since Jan 2013.

Current advancements in self-assembling nanocarriers-based siRNA delivery for cancer therapy
Colloids Surf B Biointerfaces. 2022 Nov 2;221:113002. doi: 10.1016/j.colsurfb.2022.113002. Online ahead of print.ABSTRACTDifferent therapeutic practices for treating cancers have significantly evolved to compensate and/or overcome the failures in conventional methodologies. The demonstrated potentiality in completely inhibiting the tumors and in preventing cancer relapse has made nucleic acids therapy (NAT)/gene therapy as an attractive practice. This has been made possible because NAT-based cancer treatments are highly focused on the fundamental mechanisms - i.e., silencing the expression of oncogenic genes responsible fo...
Source: Colloids and Surfaces - November 12, 2022 Category: Biotechnology Authors: Ganeshlenin Kandasamy Dipak Maity Source Type: research

Multifunctional magnetic nanocarriers for delivery of siRNA and shRNA plasmid to mammalian cells: Characterization, adsorption and release behaviors
This study introduces a new MNP functionalization that can be used for the magnetically driven intracellular delivery of nucleic acids.PMID:36162177 | DOI:10.1016/j.colsurfb.2022.112861
Source: Colloids and Surfaces - September 26, 2022 Category: Biotechnology Authors: Chi-Hsien Liu Cheng-Han Lin Yi-Jun Chen Wei-Chi Wu Chun-Chao Wang Source Type: research

Chondroitin sulfate modified chitosan nanoparticles as an efficient and targeted gene delivery vehicle to chondrocytes
In conclusion, CH-CS nanoparticles can be considered as a candidate for gene therapy purposes in cartilage diseases.PMID:36049252 | DOI:10.1016/j.colsurfb.2022.112786
Source: Colloids and Surfaces - September 1, 2022 Category: Biotechnology Authors: Naghmeh Akbari Moghadam Fatemeh Bagheri Mohamadreza Baghaban Eslaminejad Source Type: research

Novel nanocarriers for silencing anti-phagocytosis CD47 marker in acute myeloid leukemia cells
Colloids Surf B Biointerfaces. 2022 Jun 1;217:112609. doi: 10.1016/j.colsurfb.2022.112609. Online ahead of print.ABSTRACTAcute myeloid leukemia (AML), a malignant disorder of Hematopoietic stem cells, can escape immunosurveillance by over expression of the cluster of differentiation 47 (CD47) marker, which functions as an inhibitory signal, suppressing phagocytosis by binding to signal regulatory protein α (SIRPα) on macrophages. AML is treated mainly by chemotherapy, which has drastic side effects and poor outcomes for the patients. Most AML patients develop drug resistance, so other methods to treat AML are highly requ...
Source: Colloids and Surfaces - June 6, 2022 Category: Biotechnology Authors: Eman M Hassan Shan Zou Source Type: research

Host-Guest Interaction-Based Dual response core/shell nanoparticles as efficient siRNA carrier for killing breast cancer cells
Colloids Surf B Biointerfaces. 2021 Jun 11;205:111918. doi: 10.1016/j.colsurfb.2021.111918. Online ahead of print.ABSTRACTHow to overcome multiple obstacles to achieve the efficient and safe delivery of therapeutic genes is still the key to gene therapy. To address this issue, a cationic carrier consisting of polyamide-amine (HPAA-peptide-Fc) modified by an enzyme-responsive polypeptide as the core and hyperbranched polyglycerol derivative (CD-HPG) as the shell was synthesized by self-assembly. The obtained HPAA-peptide-HPG could form the compact nanocomplex with siPlk1, thus confirming the stable load of genes and subsequ...
Source: Colloids and Surfaces - June 18, 2021 Category: Biotechnology Authors: Jinglan Liang Chengguang Wu Xiaoyan Zhou Yunfeng Shi Jun Xu Xiang Cai Tingling Fu Dong Ma Wei Xue Source Type: research

Exosomes-mediated synthetic Dicer substrates delivery for intracellular Dicer imaging detection.
In this study, we design a fluorescent labeling Dicer substrate and effectively deliver it into cell by exosomes derived from the target parent cells for intracellular Dicer expression level monitor and gene therapy. Using pre-miRNA let-7a as a model, the Dicer substrates with two terminals labeled with fluorescent and quencher group respectively was obtained by T4 RNA mediated ligase reaction from two short RNA sequences. Then, the substrate was packaged into exosomes by electroporation and delivered to target cells for intracellular dicer imaging detection. After packaging substrates into exosomes with little immunogenic...
Source: Biosensors and Bioelectronics - February 1, 2020 Category: Biotechnology Authors: Dai W, Su L, Lu H, Dong H, Zhang X Tags: Biosens Bioelectron Source Type: research

Exosomes-mediated synthetic dicer substrates delivery for intracellular dicer imaging detection
In this study, we design a fluorescent labeling Dicer substrate and effectively deliver it into cell by exosomes derived from the target parent cells for intracellular Dicer expression level monitor and gene therapy. Using pre-miRNA let-7a as a model, the Dicer substrates with two terminals labeled with fluorescent and quencher group respectively was obtained by T4 RNA mediated ligase reaction from two short RNA sequences. Then, the substrate was packaged into exosomes by electroporation and delivered to target cells for intracellular dicer imaging detection. After packaging substrates into exosomes with little immunogenic...
Source: Biosensors and Bioelectronics - December 10, 2019 Category: Biotechnology Source Type: research

Toxicological exploration of peptide-based cationic liposomes in siRNA delivery.
Abstract The toxicology of cationic liposomes was explored to advance clinical trials of liposome-mediated gene therapy through the analysis of a peptide cationic liposome with DOTAP as a positive control. We first investigated the delivery of luciferase siRNA by several peptide liposomes in mice bearing lung cancer A549 cell xenografts. Of these, a cationic liposome (CDO14) was selected for further investigation. CDO14 efficiently mediated IGF-1R-siRNA delivery and inhibited the growth of the A549 cell xenografts. The in vivo toxicity and toxicological mechanisms of the selected liposome were evaluated to assess ...
Source: Colloids and Surfaces - March 24, 2019 Category: Biotechnology Authors: Zhu Y, Meng Y, Zhao Y, Zhu J, Xu H, Zhang E, Shi L, Du L, Liu G, Zhang C, Xu X, Kang X, Zhen Y, Zhang S Tags: Colloids Surf B Biointerfaces Source Type: research

Novel cell-penetrating peptide-loaded nanobubbles synergized with ultrasound irradiation enhance EGFR siRNA delivery for triple negative Breast cancer therapy.
Abstract The lack of safe and effective gene delivery strategies remains a bottleneck for cancer gene therapy. Here, we describe the synthesis, characterization, and application of cell-penetrating peptide (CPP)-loaded nanobubbles (NBs), which are characterized by their safety, strong penetrating power and high gene loading capability for gene delivery. An epidermal growth factor receptor (EGFR)-targeted small interfering RNA (siEGFR) was transfected into triple negative breast cancer (TNBC) cells via prepared CPP-NBs synergized with ultrasound-targeted microbubble destruction (UTMD) technology. Fluorescence micro...
Source: Colloids and Surfaces - June 20, 2016 Category: Biotechnology Authors: Jing H, Cheng W, Li S, Wu B, Leng X, Xu S, Tian J Tags: Colloids Surf B Biointerfaces Source Type: research

Phenylboronic acid-functionalized polyamidoamine-mediated Bcl-2 siRNA delivery for inhibiting the cell proliferation.
In conclusion, the PPP-mediated Bcl-2 siRNA delivery could potentially be an effective platform for solving the drug resistance and further achieving the combined chemotherapy and gene therapy in tumor treatment. PMID: 27371891 [PubMed - as supplied by publisher]
Source: Colloids and Surfaces - June 19, 2016 Category: Biotechnology Authors: Wu D, Yang J, Xing Z, Han H, Wang T, Zhang A, Yang Y, Li Q Tags: Colloids Surf B Biointerfaces Source Type: research