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Specialty: Biotechnology
Source: Biotechnology Letters

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Total 16 results found since Jan 2013.

BMSCs improve TNBS-induced colitis in rats by inducing Treg differentiation by expressing PD-L1
CONCLUSIONS: We found that BMSCs can induce Treg differentiation by inhibiting the Akt/mTOR pathway via PD-L1; this significantly improved symptoms and pathology in our ulcerative colitis rat models.PMID:36261682 | DOI:10.1007/s10529-022-03307-1
Source: Biotechnology Letters - October 19, 2022 Category: Biotechnology Authors: Fei Gao Dandan Cui Dongmei Zuo Zhexing Shou Jia Yang Ting Yu Yujin Liu Si Chu Feng Zhu Chunzhu Wei Source Type: research

A novel vector-based RNAi method using mouse U6 promoter-driven shRNA expression in the filamentous fungus Blakeslea trispora
CONCLUSIONS: The results suggest that the mouse U6 promoter that drives the expression of shRNA vectors may serve as a novel tool for RNAi induction in filamentous fungi.PMID:34185215 | DOI:10.1007/s10529-021-03155-5
Source: Biotechnology Letters - June 29, 2021 Category: Biotechnology Authors: Ye Li Hui Feng Lihua Jin Xiulan Xin Qipeng Yuan Source Type: research

Mechanical loading induces HIF-1 α expression in chondrocytes via YAP.
CONCLUSION: Our results indicated that cyclic mechanical stress promoted HIF-1α stabilization and YAP is involved in mechanical stress induced HIF-1α up-regulation. PMID: 32451801 [PubMed - as supplied by publisher]
Source: Biotechnology Letters - May 24, 2020 Category: Biotechnology Authors: Jing X, Yang X, Zhang W, Wang S, Cui X, Du T, Li T Tags: Biotechnol Lett Source Type: research

Inhibitory effects of msFGFR2c on the epithelial-to-mesenchymal transition of AE2 cells in pulmonary fibrosis.
Abstract In interstitial fibrosis, alveolar epithelial type II (AE2) cells fail to repair damaged epithelium. However, whether this dysfunction is related to fibroblast growth factor (FGF) signal pathway and how it affects the fibrotic process remains unclear. In our study, the medium of the human foetal lung fibroblast cell line MRC-5 (Med) can induce epithelial-to-mesenchymal transition (EMT) in AE2 cells, we also found that TGF-β in Med can induce FGF-2 and CTGF expression in AE2 cells. TGF-β or CTGF exposure trigger a FGFR2 subtype b to c transition which can be supressed by siRNA-CTGF. All together, since F...
Source: Biotechnology Letters - March 3, 2020 Category: Biotechnology Authors: Jieming G, Liu C, Yang Y, Mo S, Yang X, Wang J Tags: Biotechnol Lett Source Type: research

BST2 promotes cell proliferation, migration and induces NF- κB activation in gastric cancer.
CONCLUSION: BST2 possesses the oncogenic potential in GC by regulating the proliferation, apoptosis, and migratory ability of GC cells, thereby BST2 could be a potential therapeutic target for the treatment of GC. PMID: 29774441 [PubMed - as supplied by publisher]
Source: Biotechnology Letters - May 17, 2018 Category: Biotechnology Authors: Liu W, Cao Y, Guan Y, Zheng C Tags: Biotechnol Lett Source Type: research

MiR-338-3p inhibits TNF- α-induced lipogenesis in human sebocytes.
CONCLUSIONS: MiR-338-3p suppresses the TNF-α-induced lipogenesis in sebocytes by targeting PREX2a and down-regulating PI3K/AKT signaling. PMID: 28597147 [PubMed - as supplied by publisher]
Source: Biotechnology Letters - June 9, 2017 Category: Biotechnology Authors: Liu J, Cao L, Feng Y, Li Y, Li T Tags: Biotechnol Lett Source Type: research

TP53INP1 3'-UTR functions as a ceRNA in repressing the metastasis of glioma cells by regulating miRNA activity.
CONCLUSIONS: TP53INP1 3'-UTR could inhibit the EMT, thus hindering the migration and invasion of glioma via acting as a ceRNA for E-cadherin. PMID: 27341836 [PubMed - as supplied by publisher]
Source: Biotechnology Letters - June 23, 2016 Category: Biotechnology Authors: Wang Y, Lin G Tags: Biotechnol Lett Source Type: research

Retinoblastoma-binding protein 2 induces epithelial-mesenchymal transition in esophageal squamous cancer cells.
CONCLUSION: RBP2 could induce EMT in esophageal cancer cells and exert a greater effect on the expression of E-cadherin in lung squamous cells than in esophageal squamous cells. PMID: 26264242 [PubMed - as supplied by publisher]
Source: Biotechnology Letters - August 12, 2015 Category: Biotechnology Authors: Qiao N, Wang S, Hu L Tags: Biotechnol Lett Source Type: research

miR-155 regulates the proliferation and cell cycle of colorectal carcinoma cells by targeting E2F2.
Abstract MicroRNAs play important roles in carcinogenesis by negatively regulating the expression of target genes. Here we explore the biological function of miR-155 and the underlying mechanism in colorectal carcinoma. We validate, for the first time, that E2F2 is a direct target of miR-155 using western blot and a luciferase reporter assay and that miR-155 regulates the proliferation and cell cycle of colorectal carcinoma cells by targeting E2F2 using siRNA technology. We also found, for the first, time that E2F2 acts as a tumor suppressor in colorectal carcinoma. Overall, miR-155 plays an important role in colo...
Source: Biotechnology Letters - May 4, 2014 Category: Biotechnology Authors: Li T, Yang J, Lv X, Liu K, Gao C, Xing Y, Xi T Tags: Biotechnol Lett Source Type: research

HMGB1 enhances embryonic neural stem cell proliferation by activating the MAPK signaling pathway.
Abstract Neural stem cells (NSCs) are involved in neural tube formation. As the high-mobility group box 1 (HMGB1) protein is involved in neurulation and is present at elevated levels in neural tube defects (NTDs) induced by hyperthermia, we have now investigated the effects of HMGB1 on proliferation, differentiation, and MAPK signaling pathways of NSCs in vitro. We constructed a lentivirus vector with HMGB1 siRNA and used it to infect NSCs. Down-regulation of HMGB1 expression was confirmed. Proliferation of NSCs was determined by MTS and nestin/BrdU double-labeling. Differentiation of NSCs was assessed using β-tu...
Source: Biotechnology Letters - April 19, 2014 Category: Biotechnology Authors: Wang L, Yu L, Zhang T, Wang L, Leng Z, Guan Y, Wang X Tags: Biotechnol Lett Source Type: research

Heat-shock protein beta 1 regulates androgen-mediated bovine myogenesis.
Abstract To elucidate the functional significance of heat-shock protein beta 1 (HSPB1) in androgen-mediated myogenesis of bovine cells, we conducted 'loss and gain of function of HSPB1' assays by siRNA inhibition and gene overexpression. siRNA inhibition of HSPB1 expression reduced the expression of desmin (a myogenic marker) and repressed the formation of myotubes in cells induced for myogenic differentiation. In contrast, overexpression of HSPB1 enhanced the expression of desmin and accelerated formation of myotubes. The loss and gain of HSPB1 function was closely associated with the expression level of androgen...
Source: Biotechnology Letters - February 22, 2014 Category: Biotechnology Authors: Zhang Q, Lee HG, Kang SK, Baik M, Choi YJ Tags: Biotechnol Lett Source Type: research

Lithium chloride inhibits TGF-β1-induced myofibroblast transdifferentiation via PI3K/Akt pathway in cultured fibroblasts from Tenon's capsule of the human eye.
Abstract Excess scarring of the conjunctiva after glaucoma filtration surgery is a major cause of failure. Transforming growth factor (TGF)-β is critically involved in post-operative scarring. Lithium inhibits TGF-β-induced gene protein expression in corneal fibroblasts and inhibits TGF-β-induced epithelial mesenchymal transition. Here, we investigated the effects of LiCl on TGF-β1-mediated signaling pathways and on myofibroblast transdifferentiation of human Tenon's capsule fibroblasts (HTFs). LiCl treatment reduced expression of TGF-β1-induced α-SMA expression in HTFs. LiCl also decreased Akt phosphorylati...
Source: Biotechnology Letters - February 22, 2014 Category: Biotechnology Authors: Chung EJ, Sohn YH, Kwon SH, Jung SA, Lee JH Tags: Biotechnol Lett Source Type: research

Development of a specific siRNA delivery system into HeLa cells using an IgG-binding fusion protein.
In this study, two repeats of Fc binding domain of protein G (C2) were used to introduce a specific antibody to PEI-based carrier of siRNA. In addition, we used the double-stranded RNA binding domain (DRBD) that can bind to siRNA. The complex, consisting of PEI, siRNA and constructed fusion protein, TrxC2DRBD including C2 and DRBD domains, could protect siRNA from RNase degradation. Furthermore, cell specific siRNA delivery into HeLa cells could be performed by the complex fusion with specific antibodies via C2 domain. PMID: 23907669 [PubMed - as supplied by publisher]
Source: Biotechnology Letters - August 2, 2013 Category: Biotechnology Authors: Bae J, Mie M, Kobatake E Tags: Biotechnol Lett Source Type: research