Development of a specific siRNA delivery system into HeLa cells using an IgG-binding fusion protein.

In this study, two repeats of Fc binding domain of protein G (C2) were used to introduce a specific antibody to PEI-based carrier of siRNA. In addition, we used the double-stranded RNA binding domain (DRBD) that can bind to siRNA. The complex, consisting of PEI, siRNA and constructed fusion protein, TrxC2DRBD including C2 and DRBD domains, could protect siRNA from RNase degradation. Furthermore, cell specific siRNA delivery into HeLa cells could be performed by the complex fusion with specific antibodies via C2 domain.
PMID: 23907669 [PubMed - as supplied by publisher]

Source: Biotechnology Letters - August 2, 2013 Category: Biotechnology Authors: Bae J, Mie M, Kobatake E Tags: Biotechnol Lett Source Type: research