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Hepatitis B virus DNA polymerase displays an anti-apoptotic effect by interacting with elongation factor 1-alpha2 in hepatoma cells.
In this study, we aimed at investigating the impact of HBV Pol on host cellular processes, mainly apoptosis, and the underlying mechanisms. We showed a marked reduction in apoptotic rates in the HBV Pol-expressed HepG2 cells compared to controls. Moreover, a series of assays-i.e. yeast two-hybrid, GST pull-down, co-immunoprecipitation, and confocal laser scanning microscopy-identified the host factor eEF1A2 to be associated with HBV Pol. Furthermore, knockdown of the eEF1A2 gene by siRNA abrogated the HBV Pol-mediated anti-apoptotic effect with apoptosis induced by endoplasmatic reticulum (ER) stress inducer thapsigargin (...
Source: Journal of Microbiology and Biotechnology - October 23, 2020 Category: Biotechnology Authors: Niu X, Nong S, Gong J, Zhang X, Tang H, Zhou T, Li W Tags: J Microbiol Biotechnol Source Type: research

Identification of a Novel 1,2 Oxazine That Can Induce Apoptosis by Targeting NF-κB in Hepatocellular Carcinoma Cells
Publication date: Available online 19 February 2020Source: Biotechnology ReportsAuthor(s): Chaithanya Somu, Chakrabhavi Dhananjaya Mohan, Sachin Ambedkar, Dukanya, Shobith Rangappa, CP Baburajeev, Alexey Sukhorukov, Srishti Mishra, Muthu K Shanmugam, Arunachalam Chinnathambi, Tahani Awad Alahmadi, Sulaiman Ali Alharbi, Basappa, Kanchugarakoppal S. RangappaAbstractConstitutive activation of NF-κB is associated with proinflammatory diseases and suppression of the NF-κB signaling pathway has been considered as an effective therapeutic strategy in the treatment of various cancers including hepatocellular carcinoma (HCC). Her...
Source: Biotechnology Reports - February 20, 2020 Category: Biotechnology Source Type: research

Co-delivery of sorafenib and VEGF-siRNA via pH-sensitive liposomes for the synergistic treatment of hepatocellular carcinoma.
Authors: Yao Y, Wang T, Liu Y, Zhang N Abstract Non-viral nanocarrier affords a platform for drug and siRNA combination, the focus of which is to load drug and siRNA into a single carrier, allowing for co-delivery and a synergistic effect at tumour site. In our previous study, pH-sensitive carboxymethyl chitosan-modified liposomes (CMCS-SiSf-CL) were assembled for sorafenib (Sf) and Cy3-siRNA co-loaded. The present study evaluated in vitro and in vivo co-delivery of the co-loaded liposomes. Further, in vitro inhibiting hepatocellular carcinoma of the pH-sensitive sorafenib (Sf) and VEGF-siRNA co-loaded liposomes wa...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 14, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Co-delivery of sorafenib and VEGF-siRNA via pH-sensitive liposomes for the synergistic treatment of hepatocellular carcinoma
Volume 47, Issue 1, December 2019, Page 1374-1383 .
Source: Artificial Cells, Blood Substitutes, and Biotechnology - April 11, 2019 Category: Biotechnology Authors: Yao Yao Tianqi Wang Yongjun Liu Na Zhang Source Type: research

Activation of MET promotes resistance to sorafenib in hepatocellular carcinoma cells via the AKT/ERK1/2-EGR1 pathway.
In this study, we found that addition of HGF to sorafenib-treated HCC cells activated MET and re-stimulated the downstream AKT and ERK1/2 pathways. Thereby, restored sorafenib-treated HCC cells proliferation, migration and invasion ability, and rescued cells from apoptosis. In addition, we found that HGF treatment of HCC cells induced early growth response protein (EGR1) expression, which is involved in sorafenib resistance. Importantly, the HGF rescued effect in sorafenib-treated HCC cells could be abrogated by inhibiting MET activation with PHA-665752 or by downregulating EGR1 expression with small interfering RNA (siRNA...
Source: Artificial Cells, Nanomedicine and Biotechnology - January 23, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Liposome-mediated RNA interference delivery against Erk1 and Erk2 does not equally promote chemosensitivity in human hepatocellular carcinoma cell line HepG2.
CONCLUSION: Our data show that liposomal siRNA-mediated down-regulation of Erk1/2 can lead to potent chemosensitizing effects in HepG2 cells. Moreover, a higher chemosensitivity following Erk2 down-regulation than Erk1 down-regulation may be associated with the higher expression of Erk2 in human HCC. PMID: 28058860 [PubMed - as supplied by publisher]
Source: Artificial Cells, Nanomedicine and Biotechnology - January 7, 2017 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research