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Higher gestational weight gain delays wound healing and reduces expression of long non-coding RNA KLRK1-AS1 in neonatal endothelial progenitor cells
J Physiol. 2023 Jul 20. doi: 10.1113/JP284871. Online ahead of print.ABSTRACTHigh gestational weight gain (GWG) is a cardiovascular risk factor and may disturb neonatal endothelial function. Long non-coding RNAs (lncRNAs) regulate gene expression epigenetically and can modulate endothelial function. Endothelial colony forming cells (ECFCs), circulating endothelial precursors, are a recruitable source of endothelial cells and sustain endothelial function, vascular growth and repair. We here investigated whether higher GWG affects neonatal ECFC function and elucidated the role of lncRNAs herein. Wound healing of umbilical co...
Source: The Journal of Physiology - July 20, 2023 Category: Physiology Authors: Elisa Weiss Anna Schr üfer Carolina Tocantins Mariana Simoes Diniz Boris Novakovic Anke S van Bergen Azra Kulovic-Sissawo Richard Saffery Reinier A Boon Ursula Hiden Source Type: research

Coprophagy Prevention Decreases the Reproductive Performance and Granulosa Cell Apoptosis via Regulation of CTSB Gene in Rabbits
In conclusion, our study demonstrated the detrimental effect on reproduction by preventing coprophagy with a main role for this response played by CTSB on the granulosa cells of the ovary.
Source: Frontiers in Physiology - July 18, 2022 Category: Physiology Source Type: research

KLB dysregulation mediates disrupted muscle development in intrauterine growth restriction
In conclusion, our results identify KLB as a novel and potentially critical mediator of impaired muscle development in IUGR, through conserved mechanisms in pigs and humans. Our data sheds new light into the pathogenesis of IUGR, a significant cause of lifelong ill-health in humans and animals. Abstract figure legend In IUGR fetuses, reduced placental supply induces an adaptive response characterized by preferential shunting of blood and therefore oxygen and nutrients to vital tissues such as brain and heart, at the expense of other tissues including skeletal muscle. Using a pig model, we found skeletal muscle from IUGR fe...
Source: The Journal of Physiology - January 26, 2022 Category: Physiology Authors: Yennifer Cortes-Araya Claire Stenhouse Mazdak Salavati Susan O Dan-Jumbo William Ho Cheryl J Ashworth Emily Clark Cristina L Esteves F Xavier Donadeu Source Type: research

GLUT4 in Mouse Endometrial Epithelium: Roles in Embryonic Development and Implantation
In this study, we observed that GLUT4 exhibits a spatiotemporal expression in mouse uterus on pregnant days 1–4; its expression especially increased on pregnant day 4 during the window of implantation. We also determined that estrogen, in conjunction with progesterone, promotes the expression of GLUT4 in the endometrial epithelium in vivo or in vitro. GLUT4 is an important transporter that mediates glucose transport in endometrial epithelial cells (EECs) in vitro or in vivo. In vitro, glucose uptake decreased in mouse EECs when the cells were treated with GLUT4 small interfering RNA (siRNA). In vivo, the injection of GLU...
Source: Frontiers in Physiology - June 25, 2021 Category: Physiology Source Type: research

Bmal1 promotes prostaglandin E2 synthesis by up-regulating Ptgs2 transcription in response to increasing estradiol levels in day 4 pregnant mice.
Abstract Prostaglandin G/H synthase 2 (Ptgs2) is a rate-limiting enzyme in prostaglandin synthesis. The present study assessed the role of the uterine circadian clock on Ptgs2 transcription in response to steroid hormones during early pregnancy. We demonstrated that the core clock genes (Bmal1, Per2, Nr1d1, and Dbp), Vegf, and Ptgs2, and their encoded proteins, have rhythmic expression in the mouse uterus from days 3.5 to 4.5 (D3.5-4.5) of pregnancy. Progesterone (P4) treatment of cultured uterus endometrial stromal cells (UESCs) isolated from mPer2Luciferase reporter gene knock-in mice on D4 induced a phase shift...
Source: American Journal of Physiology. Endocrinology and Metabolism - February 8, 2021 Category: Physiology Authors: Zhao L, Yang L, Zhang J, Xiao Y, Wu M, Ma T, Wang X, Zhang L, Jiang H, Chao HW, Wang A, Jin Y, Chen H Tags: Am J Physiol Endocrinol Metab Source Type: research

GNA11 differentially mediates FGF2- and VEGFA-induced cellular responses in human fetoplacental endothelial cells.
This article is protected by copyright. All rights reserved. PMID: 29659033 [PubMed - as supplied by publisher]
Source: The Journal of Physiology - April 16, 2018 Category: Physiology Authors: Zou QY, Zhao YJ, Li H, Wang XZ, Liu AX, Zhong XQ, Yan Q, Li Y, Zhou C, Zheng J Tags: J Physiol Source Type: research

The nuclear receptor REV-ERBα represses the transcription of growth/differentiation factor 10 and 15 genes in rat endometrium stromal cells.
Abstract Cellular oscillators in the uterus play critical roles in the gestation processes of mammals through entraining of the clock proteins to numerous downstream genes, including growth/differentiation factor (Gdf)10 and Gdf15. The expression of Gdf10 and Gdf15 is significantly increased in the uterus during decidualization, but the mechanism underlying the regulation of Gdf gene expression in the uterus is poorly understood. Here, we focused on the function of the cellular oscillators in the expression of Gdf family by using uterine endometrial stromal cells (UESCs) isolated from pregnant Per2-dLuc transgenic...
Source: Physiological Reviews - January 28, 2016 Category: Physiology Authors: Zhao L, Isayama K, Chen H, Yamauchi N, Shigeyoshi Y, Hashimoto S, Hattori MA Tags: Physiol Rep Source Type: research

The nuclear receptor REV‐ERBα represses the transcription of growth/differentiation factor 10 and 15 genes in rat endometrium stromal cells
Abstract Cellular oscillators in the uterus play critical roles in the gestation processes of mammals through entraining of the clock proteins to numerous downstream genes, including growth/differentiation factor (Gdf)10 and Gdf15. The expression of Gdf10 and Gdf15 is significantly increased in the uterus during decidualization, but the mechanism underlying the regulation of Gdf gene expression in the uterus is poorly understood. Here, we focused on the function of the cellular oscillators in the expression of Gdf family by using uterine endometrial stromal cells (UESCs) isolated from pregnant Per2‐dLuc transgenic rats. ...
Source: Physiological Reports - January 26, 2016 Category: Physiology Authors: Lijia Zhao, Keishiro Isayama, Huatao Chen, Nobuhiko Yamauchi, Yasufumi Shigeyoshi, Seiichi Hashimoto, Masa‐aki Hattori Tags: Original Research Source Type: research

Removal of REV-ERBα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells.
This study focused on Ptgs2, which is essential for decidualization, as a putative clock-controlled gene, and aimed to reveal the functions of clock genes in relation to Ptgs2 during decidualization. We compared the transcript levels of clock genes in the rat uterus on days 4.5 (D4.5) and 6.5 of pregnancy. The transcript levels of clock genes (Per2, Bmal1, Rorα and Rev-erbα) had decreased at implantation sites on day 6.5 (D6.5e) compared to those on D4.5, while Ptgs2 transcripts had increased on D6.5e. Similar observations of REV-ERBα and PTGS2 were also obtained in the endometrium on D6.5e by immunohistochemistry. In t...
Source: Physiological Reviews - February 3, 2015 Category: Physiology Authors: Isayama K, Zhao L, Chen H, Yamauchi N, Shigeyoshi Y, Hashimoto S, Hattori MA Tags: Am J Physiol Endocrinol Metab Source Type: research

Differential regulation of GLUT1 and GLUT8 expression by hypoxia in mammary epithelial cells
In conclusion, the mammary glands in pregnant and lactating animals are hypoxic, and MECs respond to this hypoxia by increasing GLUT1 expression and glucose uptake through a HIF-1α-dependent mechanism. GLUT8 expression, however, is negatively regulated by hypoxia through a HIF-1α-independent pathway. The regulation of glucose transporters through hypoxia-mediated gene transcription in the mammary gland may provide an important physiological mechanism for MECs to meet the metabolic demands of mammary development and lactation.
Source: AJP: Regulatory, Integrative and Comparative Physiology - August 1, 2014 Category: Physiology Authors: Shao, Y., Wellman, T. L., Lounsbury, K. M., Zhao, F.-Q. Tags: Call for Papers Source Type: research

Differential regulation of GLUT1 and GLUT8 expression by hypoxia in mammary epithelial cells.
In conclusion, the mammary glands in pregnant and lactating animals are hypoxic, and MECs respond to this hypoxia by increasing GLUT1 expression and glucose uptake through a HIF-1α-dependent mechanism. GLUT8 expression, however, is negatively regulated by hypoxia through a HIF-1α-independent pathway. The regulation of glucose transporters through hypoxia-mediated gene transcription in the mammary gland may provide an important physiological mechanism for MECs to meet the metabolic demands of mammary development and lactation. PMID: 24920730 [PubMed - as supplied by publisher]
Source: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology - June 11, 2014 Category: Physiology Authors: Shao Y, Wellman TL, Lounsbury KM, Zhao FQ Tags: Am J Physiol Regul Integr Comp Physiol Source Type: research