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hTERT-molecular targeted therapy of ovarian cancer cells via folate-functionalized PLGA nanoparticles co-loaded with MNPs/siRNA/wortmannin
Publication date: 15 July 2021Source: Life Sciences, Volume 277Author(s): Somayyeh Ghareghomi, Shahin Ahmadian, Nosratollah Zarghami, Salar Hemmati
Source: Life Sciences - May 17, 2021 Category: Biology Source Type: research

TEX19 promotes ovarian carcinoma progression and is a potential target for epitope vaccine immunotherapy
This study aimed to reveal the role of TEX19 in ovarian cancer (OC) and searched for potential candidate epitope peptides of TEX19 to facilitate clinical application.Main methodsTEX19 levels were evaluated by immunohistochemistry (IHC) in 98 human ovarian tissue samples. The correlation of TEX19 levels with patients' clinicopathological features was assessed. Quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting analysis were utilized to detect TEX19 levels in ovarian cell lines and TEX19-deficient cells. The level of TEX19 in OVCAR-3 and A2780 was knocked down by small interfering RNA (siRNA), an...
Source: Life Sciences - December 14, 2019 Category: Biology Source Type: research

miRNA-21 Inhibition suppresses the human epithelial ovarian cancer by targeting PTEN signal pathway
ConclusionThe suppression of microRNA-21 inhibits the progression of EOC profoundly. In EOC, miR-21 is negatively correlated with the expression of PTEN gene.
Source: Saudi Journal of Biological Sciences - August 10, 2019 Category: Biology Source Type: research

Enhanced targeting of mitochondrial peroxide defense by the combined use of thiosemicarbazones and inhibitors of thioredoxin reductase.
Abstract Peroxiredoxin-3 (Prx3) accounts for about 90% of mitochondrial peroxidase activity, and its marked upregulation in many cancers is important for cell survival. Prx3 oxidation can critically alter peroxide signaling and defense and can be a seminal event in promoting cell death. Here it is shown that this mechanism can be exploited pharmacologically by combinations of clinically available drugs that compromise Prx3 function in different ways. Clinically relevant levels of the thiosemicarbazone iron chelators triapine (Tp) and 2,2'-Dipyridyl-N,N-dimethylsemicarbazone (Dp44mT) promote selective oxidation of ...
Source: Free Radical Biology and Medicine - December 11, 2015 Category: Biology Authors: Myers CR Tags: Free Radic Biol Med Source Type: research

The superoxide dismutase 1 (SOD1) 3'UTR maintains High expression of the SOD1 gene in Cancer cells: The involvement of the RNA binding protein AUF1.
In this study, a full-length 3'UTR of the SOD1 transcript was cloned and characterized for its ability to regulate SOD1 gene expression in human cancer cells. Inclusion of the SOD1 3'UTR in the pGL3 reporter construct dramatically enhanced the reporter activity by 10-220-fold in various cell lines. RT-PCR analysis, however, indicated that the reporter gene mRNA levels were only modestly altered by the SOD1 3'UTR, suggesting that SOD1 3'UTR enhances the reporter gene activity not simply by stabilizing the mRNA but primarily through promoting translation of the protein. Bioinformatics analysis showed multiple stem and loop s...
Source: Free Radical Biology and Medicine - April 20, 2015 Category: Biology Authors: Zhang S, Xue J, Zheng J, Wang S, Zhou J, Jiao Y, Geng Y, Wu J, Hannafon BN, Ding WQ Tags: Free Radic Biol Med Source Type: research

Mathematical Model of a Telomerase Transcriptional Regulatory Network Developed by Cell-Based Screening: Analysis of Inhibitor Effects and Telomerase Expression Mechanisms
In this report, we used transfection screening to test interactions among 14 TERT regulatory transcription factors and their respective promoters in ovarian cancer cells. The results were used to generate a network model of TERT transcription and to implement a dynamic Boolean model whose steady states were analysed. Modelled effects of signal transduction inhibitors successfully predicted TERT repression by Src-family inhibitor SU6656 and lack of repression by ERK inhibitor FR180204, results confirmed by RT-QPCR analysis of endogenous TERT expression in treated cells. Modelled effects of GSK3 inhibitor 6-bromoindirubin-3â...
Source: PLoS Computational Biology - February 13, 2014 Category: Biology Authors: Alan E. Bilsland et al. Source Type: research

Loss of p53 in stromal fibroblasts promotes epithelial cell invasion through redox-mediated ICAM1 Signal.
Abstract Tumor microenvironment plays a major role in cancer development. Understanding how the stroma affects epithelial transformation will provide a basis for new preventive strategies. Recent evidences suggest that oxidative stress in stroma may play a role in cancer progression and loss of p53 function in the stromal cells were associated with poor prognosis and high tumor recurrence. However, the underlying mechanisms remain poorly understood. Here, we investigated the role of p53 loss in fibroblasts on epithelial transformation and the mechanistic involvement of reactive species. Using 3D-organotypic cultur...
Source: Free Radical Biology and Medicine - January 29, 2013 Category: Biology Authors: Trachootham D, Chen G, Zhang W, Lu W, Zhang H, Liu J, Huang P Tags: Free Radic Biol Med Source Type: research