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Total 1197 results found since Jan 2013.
Genome-wide Screen for ApoA-I Secretion Lipids
Control of plasma cholesterol levels is a major therapeutic strategy for management of coronary artery disease (CAD). Although reducing LDL cholesterol (LDL-c) levels decreases morbidity and mortality, this therapeutic intervention only translates into a 25–40% reduction in cardiovascular events. Epidemiological studies have shown that a high LDL-c level is not the only risk factor for CAD; low HDL cholesterol (HDL-c) is an independent risk factor for CAD. Apolipoprotein A-I (ApoA-I) is the major protein component of HDL-c that mediates reverse cholesterol transport from tissues to the liver for excretion. Therefore, inc...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Miles, R. R., Perry, W., Haas, J. V., Mosior, M. K., N'Cho, M., Wang, J. W. J., Yu, P., Calley, J., Yue, Y., Carter, Q., Han, B., Foxworthy, P., Kowala, M. C., Ryan, T. P., Solenberg, P. J., Michael, L. F. Tags: Lipids Source Type: research
Selective Effects of Emi1 Depletion in Cancer Cell Biology
Chemotherapy and radiation in addition to surgery has proven useful in a number of different cancer types, but the effectiveness in normal tissue cannot be avoided in these therapies. To improve the effectiveness of these therapies selectively in cancer tissue is important for avoiding side effects. Early mitotic inhibitor 1 (Emi1) is known to have the function to inhibit anaphase-promoting complex/cyclosome ubiquitin ligase complex, which ubiquitylates the cell cycle-related proteins. It recently has been shown that Emi1 knockdown prevents transition from S to G2 phase by down-regulating geminin via anaphase-promoting com...
Source: Journal of Biological Chemistry - June 14, 2013 Category: Chemistry Authors: Shimizu, N., Nakajima, N. I., Tsunematsu, T., Ogawa, I., Kawai, H., Hirayama, R., Fujimori, A., Yamada, A., Okayasu, R., Ishimaru, N., Takata, T., Kudo, Y. Tags: Molecular Bases of Disease Source Type: research
Systematic Analysis of RNAi Reports Identifies Dismal Commonality at Gene-Level & Reveals an Unprecedented Enrichment in Pooled ShRNA Screens.
Systematic Analysis of RNAi Reports Identifies Dismal Commonality at Gene-Level & Reveals an Unprecedented Enrichment in Pooled ShRNA Screens.
Comb Chem High Throughput Screen. 2013 Jul 8;
Authors: Bhinder B, Djaballah H
Abstract
RNA interference (RNAi) has opened promising avenues to better understand gene function. Though many RNAi screens report on the identification of genes, very few, if any, have been further studied and validated. Data discrepancy is emerging as one of RNAi main pitfalls. We reasoned that a systematic analysis of lethality-based screens, since they score for cell death, would ex...
Source: Combinatorial Chemistry and High Throughput Screening - July 8, 2013 Category: Chemistry Authors: Bhinder B, Djaballah H Tags: Comb Chem High Throughput Screen Source Type: research
EB1 Is Required for Osteoblast Differentiation Cell Biology
Osteoblasts are differentiated mesenchymal cells that function as the major bone-producing cells of the body. Differentiation cues including ascorbic acid (AA) stimulation provoke intracellular changes in osteoblasts leading to the synthesis of the organic portion of the bone, which includes collagen type I α1, proteoglycans, and matrix proteins, such as osteocalcin. During our microarray analysis of AA-stimulated osteoblasts, we observed a significant up-regulation of the microtubule (MT) plus-end binding protein, EB1, compared with undifferentiated osteoblasts. EB1 knockdown significantly impaired AA-induced osteoblast ...
Source: Journal of Biological Chemistry - July 26, 2013 Category: Chemistry Authors: Pustylnik, S., Fiorino, C., Nabavi, N., Zappitelli, T., da Silva, R., Aubin, J. E., Harrison, R. E. Tags: Cell Biology Source Type: research
Role of Annexin A5 in Cisplatin-induced Toxicity Cell Biology
In this study, we aimed to verify the function of annexin A5 in the apoptosis of renal epithelial cells. Real-time PCR and Western blot analysis, together with immunofluorescence analysis, showed that the expression of annexin A5 significantly increased in the presence of cisplatin in both human and rat renal epithelial cells. With regard to the mechanism of cisplatin-induced apoptosis, apoptosis-inducing factor (AIF) release into the cytosol was observed, and the underlying mechanism was identified as voltage-dependent anion channel (VDAC) oligomerization. Mitochondrial membrane potential (Δψm) was found to be greatly d...
Source: Journal of Biological Chemistry - January 24, 2014 Category: Chemistry Authors: Jeong, J.-J., Park, N., Kwon, Y.-J., Ye, D.-J., Moon, A., Chun, Y.-J. Tags: Molecular Bases of Disease Source Type: research
Structure and kinetics of lipid-nucleic acid complexes.
Abstract
The structure and function of lipid-based complexes (lipoplexes) have been widely investigated as cellular delivery vehicles for nucleic acids-DNA and siRNA. Transfection efficiency in applications such as gene therapy and gene silencing has been clearly linked to the local, nano-scale organization of the nucleic acid in the vehicle, as well as to the global properties (e.g. size) of the carriers. This review focuses on both the structure of DNA and siRNA complexes with cationic lipids, and the kinetics of structure evolution during complex formation. The local organization of the lipoplexes is largely se...
Source: Advances in Colloid and Interface Science - January 28, 2014 Category: Chemistry Authors: Dan N, Danino D Tags: Adv Colloid Interface Sci Source Type: research
Syntaxin 8 Regulates Trafficking of Claudin-16 Membrane Biology
Claudin-16 (CLDN16) regulates the paracellular reabsorption of Mg2+ in the thick ascending limb of Henle's loop. However, the mechanism regulating the tight junctional localization of CLDN16 remains unknown. In yeast two-hybrid systems, we found that CLDN16 bound to syntaxin 8 (STX8), a target soluble N-ethylmaleimide-sensitive factor attachment protein receptor. We have examined the effect of STX8 on the localization and function of CLDN16 using Madin-Darby canine kidney cells expressing FLAG-tagged CLDN16. A pulldown assay showed that the carboxyl cytoplasmic region of human CLDN16 bound to STX8. CLDN16 was localized in ...
Source: Journal of Biological Chemistry - May 8, 2014 Category: Chemistry Authors: Ikari, A., Tonegawa, C., Sanada, A., Kimura, T., Sakai, H., Hayashi, H., Hasegawa, H., Yamaguchi, M., Yamazaki, Y., Endo, S., Matsunaga, T., Sugatani, J. Tags: Cell Biology Source Type: research
Wnt9A Expression Increased by Decline in Arylsulfatase B Molecular Bases of Disease
In this study, the novel transcriptional mechanism by which carrageenan and decline in ARSB increase Wnt9A expression in NCM460 and HT-29 human colonic epithelial cells and in mouse colon is presented. Increased expression of Wnt9A has been associated with multiple malignancies, including colon carcinoma, and with ectodermal and mesoendodermal morphogenesis. When ARSB activity was reduced by siRNA or by exposure to carrageenan (1 μg/ml for 24 h), degradation of chondroitin 4-sulfate (C4S) was inhibited, leading to accumulation of more highly sulfated C4S, which binds less galectin-3, a β-galactoside-binding protein. Nucl...
Source: Journal of Biological Chemistry - June 19, 2014 Category: Chemistry Authors: Bhattacharyya, S., Feferman, L., Tobacman, J. K. Tags: Glycobiology and Extracellular Matrices Source Type: research
Dependence of Hepatic Stellate Cells on Collagen Glycobiology and Extracellular Matrices
Stellate cells are distributed throughout organs, where, upon chronic damage, they become activated and proliferate to secrete collagen, which results in organ fibrosis. An intriguing property of hepatic stellate cells (HSCs) is that they undergo apoptosis when collagen is resolved by stopping tissue damage or by treatment, even though the mechanisms are unknown. Here we disclose the fact that HSCs, normal diploid cells, acquired dependence on collagen for their growth during the transition from quiescent to active states. The intramolecular RGD motifs of collagen were exposed by cleavage with their own membrane type 1 mat...
Source: Journal of Biological Chemistry - July 17, 2014 Category: Chemistry Authors: Birukawa, N. K., Murase, K., Sato, Y., Kosaka, A., Yoneda, A., Nishita, H., Fujita, R., Nishimura, M., Ninomiya, T., Kajiwara, K., Miyazaki, M., Nakashima, Y., Ota, S., Murakami, Y., Tanaka, Y., Minomi, K., Tamura, Y., Niitsu, Y. Tags: Cell Biology Source Type: research
EDD Influences Mitosis Signal Transduction
In this work, we identify physical and genetic interactions that implicate E3 identified by differential display (EDD) in promoting spindle assembly checkpoint (SAC) function. During mitosis, the SAC initiates a mitotic checkpoint in response to chromosomes with kinetochores unattached to spindle pole microtubules. Similar to Budding uninhibited by benzimidazoles-related 1 (BUBR1) siRNA, a bona fide SAC component, EDD siRNA abrogated G2/M accumulation in response to the mitotic destabilizing agent nocodazole. Furthermore, EDD siRNA reduced mitotic cell viability and, in nocodazole-treated cells, increased expression of the...
Source: Journal of Biological Chemistry - May 14, 2015 Category: Chemistry Authors: Scialpi, F., Mellis, D., Ditzel, M. Tags: Cell Biology Source Type: research
Fluorescein labelled cationic carbosilane dendritic systems for biological studies
Publication date: October 2015 Source:European Polymer Journal, Volume 71 Author(s): Elena Fuentes-Paniagua, M Jesús Serramía, Javier Sánchez-Nieves, Susana Álvarez, M Ángeles Muñoz-Fernández, Rafael Gómez, F. Javier de la Mata Cationic carbosilane dendrimers and dendrons labelled with one fluorescein unit have been synthesized. For dendrimers (generations 1–3), a random procedure was followed by successive addition of two types of thiol compounds to vinyl terminated derivatives, first one with –NH3Cl and second one with –NMe2HCl functions, subsequent reaction with FITC and finally quaternization wit...
Source: European Polymer Journal - July 31, 2015 Category: Chemistry Source Type: research
A Multi-RNAi Microsponge Platform for Simultaneous Controlled Delivery of Multiple Small Interfering RNAs.
We present a novel RNA nanotechnology based approach to produce multiple components of polymerized siRNA molecules that are simultaneously self-assembled and densely packaged into composite sponge-like porous microstructures (Multi-RNAi-MSs) by rolling circle transcription. The Multi-RNAi-MSs were designed to contain a combination of multiple polymeric siRNA molecules with precisely controlled stoichiometry within a singular microstructure by manipulating the types and ratios of the circular DNA templates. The Multi-RNAi-MSs were converted into nanosized complexes by polyelectrolyte condensation to manipulate their physico...
Source: Angewandte Chemie - December 22, 2015 Category: Chemistry Authors: Roh YH, Deng JZ, Dreaden EC, Park JH, Yun DS, Shopsowitz KE, Hammond PT Tags: Angew Chem Int Ed Engl Source Type: research
STAT3-dependent Regulation of PCSK9 Molecular Bases of Disease
In conclusion, our data candidate PCSK9 as a gene involved in lipid metabolism regulated by proinflammatory cytokine TNF-α in a SOCS3-dependent manner.
Source: Journal of Biological Chemistry - February 12, 2016 Category: Chemistry Authors: Ruscica, M., Ricci, C., Macchi, C., Magni, P., Cristofani, R., Liu, J., Corsini, A., Ferri, N. Tags: Cell Biology Source Type: research
Self-assembled Micelle iRNA in Pulmonary Fibrosis Molecular Bases of Disease
This study demonstrates that SAMiRNA nanoparticle is a less toxic, stable siRNA silencing platform for efficient in vivo targeting of genes implicated in the pathogenesis of pulmonary fibrosis.
Source: Journal of Biological Chemistry - March 18, 2016 Category: Chemistry Authors: Yoon, P. O., Park, J. W., Lee, C.-M., Kim, S. H., Kim, H.-N., Ko, Y., Bae, S. J., Yun, S., Park, J. H., Kwon, T., Kim, W. S., Lee, J., Lu, Q., Kang, H.-R., Cho, W.-K., Elias, J. A., Yang, J.-S., Park, H.-O., Lee, K., Lee, C. G. Tags: Gene Regulation Source Type: research
Nitrite-derived nitric oxide reduces hypoxia-inducible factor 1 α-mediated extracellular vesicle production by endothelial cells
Conclusion These data provide evidence that hypoxia enhances the release of EVs in endothelial cells, and that this is mediated by HIF-1α, but not HIF-2α. Furthermore, the reduction of NO2 − to NO via xanthine oxidoreductase during hypoxia appears to inhibit HIF-1α-mediated EV production.
Source: Nitric Oxide - December 23, 2016 Category: Chemistry Source Type: research
