Nitric oxide and abscisic acid protects against PEG-induced drought stress differentially in Brassica genotypes by combining the role of stress modulators, markers and antioxidants
Publication date: Available online 13 May 2019Source: Nitric OxideAuthor(s): Seema Sahay, Ehasanullah Khan, Meetu GuptaAbstractThe present study was designed to see the effect of exogenous nitric oxide (NO) and abscisic acid (ABA) and their interaction on physiological and biochemical activities in leaves and roots of two Indian mustard (Brassica juncea) cultivars [cv. Pusa Jagannath (PJN) and Varuna (VAR)] exposed to polyethylene glycol (PEG)-induced drought stress. Seven days old hydroponically grown seedlings were treated with PEG (10%), sodium nitroprusside, a NO donor [NO (100 μM)] and abscisic acid [ABA (10&n...
Source: Nitric Oxide - May 13, 2019 Category: Chemistry Source Type: research

Editorial Board
Publication date: 1 July 2019Source: Nitric Oxide, Volume 88Author(s): (Source: Nitric Oxide)
Source: Nitric Oxide - May 10, 2019 Category: Chemistry Source Type: research

Exogenous l-ARGININE does not stimulate production OF NO or cGMP within the rat corporal smooth muscle cells in culture
ConclusionsThis in vitro study demonstrates that exogenous administration of L-ARG or L-CIT failed to stimulate production of either NO or cGMP by the corporal CSM cells. A re-evaluation of the presumptive role of the exogenous administration of L-ARG in improving the synthesis of NO at least at the level of the CSM cells appears warranted. (Source: Nitric Oxide)
Source: Nitric Oxide - May 8, 2019 Category: Chemistry Source Type: research

NADPH oxidase is a primary target for antioxidant effects by inorganic nitrite in lipopolysaccharide-induced oxidative stress in mice and in macrophage cells
In conclusion, these novel findings position NADPH oxidase in the inflammatory vasculature as a prime target for the antioxidant effects of inorganic nitrite, and open a new direction to modulate the inflammatory response.Graphical abstractThrough mediating NADPH oxidase activity, the increase of NO bioavailability by nitrite-NO pathway will reduce vascular oxidative stress and endothelial dysfunction, resulting in higher survival during acute inflammation. (Source: Nitric Oxide)
Source: Nitric Oxide - May 5, 2019 Category: Chemistry Source Type: research

The role of nitric oxide signaling in pulmonary circulation of high- and low-altitude newborn sheep under basal and acute hypoxic conditions
Publication date: Available online 4 May 2019Source: Nitric OxideAuthor(s): Emilio A. Herrera, Germán Ebensperger, Ismael Hernández, Emilia M. Sanhueza, Aníbal J. Llanos, Roberto V. ReyesAbstractNitric oxide (NO) is the main vasodilator agent that drives the rapid decrease of pulmonary vascular resistance for the respiratory onset during the fetal to neonatal transition. Nevertheless, the enhanced NO generation by the neonatal pulmonary arterial endothelium does not prevent development of hypoxic pulmonary hypertension in species without an evolutionary story at high altitude. Therefore, this study aim...
Source: Nitric Oxide - May 5, 2019 Category: Chemistry Source Type: research

Alveolar concentration of nitric oxide as a prognostic biomarker in idiopathic pulmonary fibrosis
Publication date: Available online 2 May 2019Source: Nitric OxideAuthor(s): Paolo Cameli, Laura Bergantini, Martina Salvini, Rosa Metella Refini, Maria Pieroni, Elena Bargagli, Piersante SestiniAbstractBackgroundIdiopathic pulmonary fibrosis (IPF) is a chronic and progressive fibrotic lung disease leading to respiratory failure and death in 2–5 years from diagnosis. To date, clinical course of disease and prognosis cannot be predicted with an acceptable accuracy. Recently, alveolar concentration of nitric oxide (CaNO) has been proposed as a marker of severity of IPF, but its prognostic value in this setting is unknow...
Source: Nitric Oxide - May 4, 2019 Category: Chemistry Source Type: research

Targeting nitric oxide as a key modulator of sepsis, arthritis and pain
Publication date: Available online 30 April 2019Source: Nitric OxideAuthor(s): Fernando Spiller, Rodrigo de Oliveira Formiga, Jonathan Fernandes da Silva Coimbra, Jose Carlos Alves-Filho, Thiago Mattar Cunha, Fernando Queiroz CunhaAbstractNitric oxide (NO) is produced by enzymatic activity of neuronal (nNOS), endothelial (eNOS), and inducible nitric oxide synthase (iNOS) and modulates a broad spectrum of physiological and pathophysiological conditions. The iNOS isoform is positively regulated at transcriptional level and produces high levels of NO in response to inflammatory mediators and/or to pattern recognition receptor...
Source: Nitric Oxide - May 1, 2019 Category: Chemistry Source Type: research

Dietary nitrate supplementation alters the oral microbiome but does not improve the vascular responses to an acute nitrate dose
Publication date: Available online 30 April 2019Source: Nitric OxideAuthor(s): Mia Burleigh, Luke Liddle, David J. Muggeridge, Christopher Monaghan, Nicholas Sculthorpe, John Butcher, Fiona Henriquez, Chris EastonAbstractNitrate (NO3−) contained in food and beverages can transiently increase nitric oxide (NO) availability following a stepwise reduction to nitrite (NO2−) by commensal bacteria in the oral cavity. We tested the hypothesis that regular ingestion of dietary NO3− would influence the oral microbiome, the capacity to reduce NO3− to NO2− in saliva, and the vascular responses to an acut...
Source: Nitric Oxide - May 1, 2019 Category: Chemistry Source Type: research

Site and mechanism of uncoupling of nitric-oxide synthase: Uncoupling by monomerization and other misconceptions
Publication date: 1 August 2019Source: Nitric Oxide, Volume 89Author(s): Verena Gebhart, Katja Reiß, Alexander Kollau, Bernd Mayer, Antonius C.F. GorrenAbstractNitric oxide synthase (NOS) catalyzes the transformation of l-arginine, molecular oxygen (O2), and NADPH-derived electrons to nitric oxide (NO) and l-citrulline. Under some conditions, however, NOS catalyzes the reduction of O2 to superoxide (O2−) instead, a phenomenon that is generally referred to as uncoupling. In principle, both the heme in the oxygenase domain and the flavins in the reductase domain could catalyze O2− formation. In the former c...
Source: Nitric Oxide - May 1, 2019 Category: Chemistry Source Type: research

Characterization of plant glutamine synthetase S-nitrosation
Publication date: Available online 23 April 2019Source: Nitric OxideAuthor(s): Liliana S. Silva, Mariana Q. Alves, Ana R. Seabra, Helena G. CarvalhoAbstractThe identification of S-nitrosated substrates and their target cysteine residues is a crucial step to understand the signaling functions of nitric oxide (NO) inside the cells. Here, we show that the key nitrogen metabolic enzyme glutamine synthetase (GS) is a S-nitrosation target in Medicago truncatula and characterize the molecular determinants and the effects of this NO-induced modification on different GS isoenzymes. We found that all the four M. truncatula GS isofor...
Source: Nitric Oxide - April 25, 2019 Category: Chemistry Source Type: research

Site and mechanism of uncoupling of nitric-oxide synthase uncoupling by monomerization and other misconceptions
Publication date: Available online 22 April 2019Source: Nitric OxideAuthor(s): Verena Gebhart, Katja Reiß, Alexander Kollau, Bernd Mayer, Antonius C.F. GorrenAbstractNitric oxide synthase (NOS) catalyzes the transformation of L-arginine, molecular oxygen (O2), and NADPH-derived electrons to nitric oxide (NO) and L-citrulline. Under some conditions, however, NOS catalyzes the reduction of O2 to superoxide (O2−) instead, a phenomenon that is generally referred to as uncoupling. In principle, both the heme in the oxygenase domain and the flavins in the reductase domain could catalyze O2− formation. In the fo...
Source: Nitric Oxide - April 23, 2019 Category: Chemistry Source Type: research

Nitric oxide and interactions with reactive oxygen species in the development of melanoma, breast, and colon cancer: A redox signaling perspective
Publication date: Available online 19 April 2019Source: Nitric OxideAuthor(s): Hugo P. Monteiro, Elaine G. Rodrigues, Adriana K.C. Amorim Reis, Luiz S. Longo, Fernando T. Ogata, Ana I.S. Moretti, Paulo E. da Costa, Ana C.S. Teodoro, Maytê S. Toledo, Arnold SternAbstractCancer development is closely related to chronic inflammation, which is associated with identifiable markers of tumor progression, such as uncontrolled cell proliferation, angiogenesis, genomic instability, chemotherapeutic resistance, and metastases. Redox processes mediated by reactive oxygen species (ROS) and nitric oxide (NO) within the inflammator...
Source: Nitric Oxide - April 20, 2019 Category: Chemistry Source Type: research

Editorial Board
Publication date: 1 June 2019Source: Nitric Oxide, Volume 87Author(s): (Source: Nitric Oxide)
Source: Nitric Oxide - April 19, 2019 Category: Chemistry Source Type: research

Regulation of human inducible nitric oxide synthase expression by an upstream open reading frame
Publication date: Available online 18 April 2019Source: Nitric OxideAuthor(s): Fabian Gather, Katja Schmitz, Kathrin Koch, Lea-Marie Vogt, Andrea Pautz, Hartmut KleinertAbstractThe human inducible nitric oxide synthase (iNOS) gene contains an upstream open reading frame (uORF) in its 5′-untranslated region (5′-UTR) implying a translational regulation of iNOS expression. Transfection experiments in human DLD-1 cells revealed that the uORF although translatable seems not to inhibit the translation start at the bona fide ATG. Our data clearly show that human iNOS translation is cap-dependent and that the 5&prime...
Source: Nitric Oxide - April 19, 2019 Category: Chemistry Source Type: research

Influence of two functional polymorphisms in NOS1 on baseline cortisol and working memory in healthy subjects
ConclusionFor the first time, we demonstrated an influence of the NOS1 ex1c-SNP rs41279104 on salivary cortisol levels and additionally implicate the A-allele in an enhanced reaction time during a working memory task. Regarding the NOS1 ex1f-VNTR our study supports the previously reported influence on impulsivity, lending further support to the hypothesis that this genetic variant underlies impulsive behavior. (Source: Nitric Oxide)
Source: Nitric Oxide - April 17, 2019 Category: Chemistry Source Type: research

Reciprocal regulation of sulfite oxidation and nitrite reduction by mitochondrial sulfite oxidase
In conclusion, we propose a pH-dependent reciprocal regulation of sulfite oxidation and nitrite reduction by each substrate, thus facilitating quick responses to hypoxia induced changes in the IMS, which may function in protecting the cell from reactive oxygen species production. (Source: Nitric Oxide)
Source: Nitric Oxide - April 17, 2019 Category: Chemistry Source Type: research

The effect of nitric oxide on mitochondrial respiration
This article reviews the interactions between nitric oxide (NO) and mitochondrial respiration. Mitochondrial ATP synthesis is responsible for virtually all energy production in mammals, and every other process in living organisms ultimately depends on that energy production. Furthermore, both necrosis and apoptosis, that summarize the main forms of cell death, are intimately linked to mitochondrial integrity.Endogenous and exogenous •NO inhibits mitochondrial respiration by different well-studied mechanisms and several nitrogen derivatives. Instantaneously, low concentrations of •NO, specifically and reversibly i...
Source: Nitric Oxide - April 16, 2019 Category: Chemistry Source Type: research

Reactive nitrogen species induced catalases promote a novel nitrosative stress tolerance mechanism in Vibrio cholerae
Publication date: Available online 11 April 2019Source: Nitric OxideAuthor(s): Sourav Kumar Patra, Sourabh Samaddar, Nilanjan Sinha, Sanjay GhoshAbstractVibrio cholerae faces nitrosative stress during successful colonization in intestine. Very little information is available on the nitrosative stress protective mechanisms of V. cholerae. Reports show that norR regulon control two genes hmpA and nnrS responsible for nitric oxide (NO) detoxification in V. cholerae. In the present study we first time report a novel role V. cholerae catalases under nitrosative stress. Using zymogram analysis of catalase we showed that KatB and...
Source: Nitric Oxide - April 13, 2019 Category: Chemistry Source Type: research

Inorganic nitrate prevents the loss of tight junction proteins and modulates inflammatory events induced by broad-spectrum antibiotics: A role for intestinal microbiota?
This study investigates the impact of nitrate on gut microbiota profile and ensued mucosal effects during dysbiosis. Male Wistar rats were randomly distributed in 4 groups and the drinking water was supplemented for 7 days as follows: 1) antibiotic cocktail (neomycin, bacitracin and imipenem), 2) antibiotic cocktail + sodium nitrate, 3) sodium nitrate and 4) regular drinking water. Animals were weighted daily and feces were collected before and after the treatment. The stomach was isolated and the expression of occludin, claudin-5 as well as myeloperoxidase and iNOS was studied. Bacterial DNA was analyzed in feca...
Source: Nitric Oxide - April 11, 2019 Category: Chemistry Source Type: research

Deciphering the nitric oxide, cyanide and iron-mediated actions of sodium nitroprusside in cotyledons of salt stressed sunflower seedlings
Publication date: Available online 19 March 2019Source: Nitric OxideAuthor(s): Monika Keisham, Prachi Jain, Neha Singh, Christine von Toerne, Satish C. Bhatla, Christian LindermayrAbstractNitric oxide (NO) is an endogenous signaling molecule in plants. Sodium nitroprusside (SNP), an established NO donor used in plant science research, simultaneously releases NO, cyanide (CN−) and iron (Fe) in solution. Since cyanide and iron mask NO effect of SNP, its use in NO research is debatable. Deciphering the action of SNP through NO, CN− or Fe has been undertaken in the present work. Cotyledons from salt stressed sunflo...
Source: Nitric Oxide - March 21, 2019 Category: Chemistry Source Type: research

Angiotensin-(1-7) induced vascular relaxation in spontaneously hypertensive rats
Publication date: Available online 14 March 2019Source: Nitric OxideAuthor(s): Feng Zhang, Haiyang Tang, Shuo Sun, Yuru Luo, Xingsheng Ren, Aidong Chen, Yu Xu, Peng Li, Ying HanAbstractEnhanced vasoconstriction and decreased vasodilatation due to endothelial dysfunction contribute to the progression of hypertension. Angiotensin (Ang)-(1-7) plays important roles in regulating the cardiovascular activity. The current study aimed to investigate the roles of Ang-(1-7) in modulating blood pressure, vascular tension and its signal pathway in spontaneously hypertensive rats (SHR). The effects of intravenous injection of drugs wer...
Source: Nitric Oxide - March 15, 2019 Category: Chemistry Source Type: research

Exercise training augments neuronal nitric oxide synthase dimerization in the paraventricular nucleus of rats with chronic heart failure
Publication date: Available online 13 March 2019Source: Nitric OxideAuthor(s): Neeru M. Sharma, Xuefei Liu, Tamra L. Llewellyn, Kenichi Katsurada, Kaushik P. PatelAbstractExercise training (ExT) is an established non-pharmacological therapy that improves the health and quality of life in patients with chronic heart failure (CHF). Exaggerated sympathetic drive characterizes CHF due to an imbalance of the autonomic nervous system. Neuronal nitric oxide synthases (nNOS) in the paraventricular nucleus (PVN) produce nitric oxide (NO•), which is known to regulate the sympathetic tone. Previously we have shown that during CH...
Source: Nitric Oxide - March 15, 2019 Category: Chemistry Source Type: research

Altered neurovascular coupling and brain arginine metabolism in endothelial nitric oxide synthase deficient mice
Publication date: Available online 13 March 2019Source: Nitric OxideAuthor(s): Ashwini Hariharan, Yu Jing, Nicola D. Collie, Hu Zhang, Ping LiuAbstractNitric oxide (NO) produced by endothelial NO synthase (eNOS) is a key regulator of cerebral blood flow (CBF) dynamics. Mice with eNOS deficiency (eNOS−/-) display age-related increases in amyloid beta in the brain and memory deficits, implicating eNOS dysfunction in the neuropathogenesis and/or development of Alzheimer's disease (AD). The present study systematically investigated behavioural, CBF and brain arginine metabolic profile changes in male and female wildtype ...
Source: Nitric Oxide - March 13, 2019 Category: Chemistry Source Type: research

The origins of nitric oxide and peroxynitrite research in Uruguay: 25 years of contributions to the biochemical and biomedical sciences
Publication date: Available online 11 March 2019Source: Nitric OxideAuthor(s): Rafael RadiAbstractIn this review, I provide historical background on how nitric oxide (•NO) and peroxynitrite research was originated in Uruguay in the early 90′s and how the investigations evolved through over more than two decades within a context related to human biology. This process involved the participation of multiple local investigators, in conjunction with collaborations at the regional (Latin American) and international levels. The discoveries have been integrated with parallel investigations from other research groups wor...
Source: Nitric Oxide - March 12, 2019 Category: Chemistry Source Type: research

Increase of cystathionine-γ-lyase (CSE) during late wound repair: Hydrogen sulfide triggers cytokeratin 10 expression in keratinocytes
Publication date: Available online 9 March 2019Source: Nitric OxideAuthor(s): Itamar Goren, Yvette Köhler, Ahmed Aglan, Josef Pfeilschifter, Karl-Friedrich Beck, Stefan FrankAbstractThe gaseous mediators nitric oxide (NO), carbon monoxide (CO) and lately also hydrogen sulfide (H2S) have been described to contribute to the interplay of protein type- and lipid mediators in the regulation of wound healing. In particular, the recently reported role of H2S in skin repair remains largely unresolved. Therefore we assessed the expressional kinetics of potential H2S-producing enzymes during undisturbed skin repair: the cystath...
Source: Nitric Oxide - March 10, 2019 Category: Chemistry Source Type: research

S-nitrosylation and its role in breast cancer angiogenesis and metastasis
Publication date: Available online 9 March 2019Source: Nitric OxideAuthor(s): Pamela Ehrenfeld, Francisco Cordova, Walter N. Duran, Fabiola A. SanchezAbstractS-nitrosylation, the modification by nitric oxide of free sulfhydryl groups in cysteines, has become an important regulatory mechanism in carcinogenesis and metastasis. S-nitrosylation of targets in tumor cells contributes to metastasis regulating epithelial to mesenchymal transition, migration and invasion. In the tumor environment, the role of S-nitrosylation in endothelium has not been addressed; however, the evidence points out that S-nitrosylation of endothelial ...
Source: Nitric Oxide - March 9, 2019 Category: Chemistry Source Type: research

The oral nitrate-reducing capacity correlates with peak power output and peak oxygen uptake in healthy humans
Publication date: Available online 7 March 2019Source: Nitric OxideAuthor(s): B. Thomas, S. Smallwood, C. Cutler, R. BescosAbstractInterest in inorganic nitrate and nitrite has grown substantially over the past decade as research has revealed the role of these anions in enhancing nitric oxide (NO) availability through an oral pathway. Nitrite synthesis in the mouth seems to be an important mechanism to feed the circulatory system with this anion. This is interesting since greater plasma nitrite concentration has been associated with better fitness levels in humans, but this question has not been investigated in relation to...
Source: Nitric Oxide - March 8, 2019 Category: Chemistry Source Type: research

The role of NO-cGMP pathway inhibition in vascular endothelial-dependent smooth muscle relaxation disorder of AT1-AA positive rats: Protective effects of adiponectin
Publication date: Available online 1 March 2019Source: Nitric OxideAuthor(s): Zhiyuan Wang, Ye Wu, Suli Zhang, Yuhui Zhao, Xiaochen Yin, Wen Wang, Xinliang Ma, Huirong LiuAbstractAngiotensin II type 1 receptor autoantibodies (AT1-AA) cause endothelial-dependent smooth muscle relaxation disorder. It is well understood that impairment of the NO–cGMP signaling pathway is one of the mechanisms of endothelial-dependent smooth muscle relaxation disorder. However, it is still unclear whether AT1-AA induces endothelial-dependent smooth muscle relaxation disorder via the impairment of the NO–cGMP signaling pathway. In a...
Source: Nitric Oxide - March 5, 2019 Category: Chemistry Source Type: research

Exercise training restores eNOS activation in the perivascular adipose tissue of obese rats: Impact on vascular function
ConclusionExercise training in obese rats is able to impact PVAT eNOS with subsequent beneficial impact on vascular function.Graphical abstract (Source: Nitric Oxide)
Source: Nitric Oxide - March 5, 2019 Category: Chemistry Source Type: research

The active-site cysteine residue of Ca2+/calmodulin-dependent protein kinase I is protected from irreversible modification via generation of polysulfidation
Publication date: Available online 4 March 2019Source: Nitric OxideAuthor(s): Tsuyoshi Takata, Ayaka Tsukuda, Yukihiro Tsuchiya, Takaaki Akaike, Yasuo WatanabeAbstractCa2+/calmodulin (CaM)-dependent protein kinase (CaMK) I is activated by the phosphorylation of a crucial activation loop Thr177 by upstream kinases, CaMK kinase (CaMKK), and regulates axonal or dendritic extension and branching. Reactive sulfur species (RSS) modulate protein functions via polysulfidation of the reactive Cys residues. Here, we report that the activity of CaMKI was reversibly inhibited via its polysulfidation at Cys179 by RSS. In vitro incubati...
Source: Nitric Oxide - March 5, 2019 Category: Chemistry Source Type: research

Sodium/calcium exchanger is involved in apoptosis induced by H2S in tumor cells through decreased levels of intracellular pH
Publication date: Available online 5 March 2019Source: Nitric OxideAuthor(s): Ivan Szadvari, Sona Hudecova, Barbora Chovancova, Miroslava Matuskova, Dana Cholujova, Lubomira Lencesova, David Valerian, Karol Ondrias, Petr Babula, Olga KrizanovaAbstractWe explored possibility that sodium/calcium exchanger 1 (NCX1) is involved in pH modulation and apoptosis induction in GYY4137 treated cells. We have shown that although 10 days treatment with GYY4137 did not significantly decreased volume of tumors induced by colorectal cancer DLD1 cells in nude mice, it already induced apoptosis in these tumors. Treatment of DLD1 and ovarian...
Source: Nitric Oxide - March 5, 2019 Category: Chemistry Source Type: research

Nitration of hIAPP promotes its toxic oligomer formation and exacerbates its toxicity towards INS-1 cells
In this study, fluorescent assays, transmission electron microscopy (TEM), atomic force microscope (AFM) were used to demonstrate that nitration of hIAPP significantly decreased its fibril formation. But the decreased fibril formation was not through the diminished aggregation of hIAPP monomer as suggested by the results of circular dichroism spectroscopy (CD) and gel electrophoresis assay. Surface-enhanced raman spectroscopy (SERS) indicated that nitration of hIAPP impaired the intermolecular hydrogen bonding. On the basis of these results, we hypothesize that nitration of hIAPP may block the intermolecular hydrogen bondi...
Source: Nitric Oxide - March 5, 2019 Category: Chemistry Source Type: research

Cx46 hemichannel modulation by nitric oxide: Role of the fourth transmembrane helix cysteine and its possible involvement in cataract formation
Publication date: Available online 21 February 2019Source: Nitric OxideAuthor(s): Mauricio A. Retamal, Viviana P. Orellana, Nicolás J. Arévalo, Cristóbal G. Rojas, Rodolfo J. Arjona, Constanza A. Alcaíno, Wendy González, Jonathan G. Canan, Rodrigo Moraga-Amaro, Jimmy Stehberg, Luis Reuss, Guillermo A. AltenbergAbstractUnder normal conditions, connexin (Cx) hemichannels have a low open probability, which can increase under pathological conditions. Since hemichannels are permeable to relatively large molecules, their exacerbated activity has been linked to cell damage. Cx46 is highly expres...
Source: Nitric Oxide - February 22, 2019 Category: Chemistry Source Type: research

Editorial Board
Publication date: 1 April 2019Source: Nitric Oxide, Volume 85Author(s): (Source: Nitric Oxide)
Source: Nitric Oxide - February 22, 2019 Category: Chemistry Source Type: research

Cytotoxicity, cellular uptake, and subcellular localization of a nitrogen oxide and aminopropyl-β-lactose derivative ruthenium complex used as nitric oxide delivery agent
Publication date: Available online 19 February 2019Source: Nitric OxideAuthor(s): Joicy S. dos Santos, Loyanne C. Ramos, Lucimara P. Ferreira, Vanessa Campos, Lucas C.D. de Rezende, Flávio da Silva Emery, Roberto Santana da SilvaAbstractThis work investigates how the luminescent ruthenium-nitrite complexes cis-[Ru(py-bodipy)(dcbpy)2(NO2)](PF6) (I) and cis-[Ru(py-bodipy)(dcbpy-aminopropyl-β-lactose)2(NO2)](PF6) (II) behave toward the melanoma cancer cell line B16F10. The chemical structure and purity of the synthesized complexes were analyzed by UV-Visible and FTIR spectroscopy, MALDI, HPLC, and 1H NMR. Spectrof...
Source: Nitric Oxide - February 19, 2019 Category: Chemistry Source Type: research

The effect of passive smoking on exhaled nitric oxide in asthmatic children
Publication date: Available online 16 February 2019Source: Nitric OxideAuthor(s): M. Bobrowska-Korzeniowska, I. Stelmach, A. Brzozowska, J. Jerzyńska, M. Mitał, W. StelmachAbstractTo date, some studies suggest that passive smoking (PS) may be an important determinant of FeNO levels in children but sill there is a need of investigations using objective methods of PS exposure.The aim of our study was to examine the effect of PS, measured by urine cotinine levels, on FeNO and lung function (FEV1) in allergic and non-allergic asthmatic children.MethodsIt was a prospective, non-interventional study. 140 children, aged 4&ndash...
Source: Nitric Oxide - February 17, 2019 Category: Chemistry Source Type: research

NO donors induce vascular relaxation by different cellular mechanisms in hypertensive and normotensive rats
ConclusionOur results indicate that SNP and RuBPY-induced mesenteric resistance artery relaxation involves NO/sGC/cGMP/PKG pathway activation. K+ channels and SERCA activation is required to SNP but not for RuBPY-induced relaxation. Moreover, SERCA seems to be impaired in hypertensive 2K-1C rat mesenteric resistance arteries although it does not impact SNP- or RuBPY-induced relaxation.Graphical abstract (Source: Nitric Oxide)
Source: Nitric Oxide - February 15, 2019 Category: Chemistry Source Type: research

Cross-talk between the Ras GTPase and the Hog1 survival pathways in response to nitrosative stress in Paracoccidioides brasiliensis
In this report, we investigated the role of Ras GTPase in P. brasiliensis after in vitro stimulus with nitric oxide (NO). We observed that low concentrations of NO induced cell proliferation in P. brasiliensis, while high concentrations promoted decrease in fungal viability, and both events were reversed in the presence of a NO scavenger. We observed that high levels of NO induced Ras activation and its S-nitrosylation. Additionally, we showed that Ras modulated the expression of antioxidant genes in response to nitrosative stress. We find that the Hog1 MAP kinase contributed to nitrosative stress response in P. brasiliens...
Source: Nitric Oxide - February 15, 2019 Category: Chemistry Source Type: research

Effect of long-term sodium nitrate administration on diabetes-induced anemia and glucose homeostasis in obese type 2 diabetic male rats
ConclusionLong-term and low dose of nitrate had beneficial effects against anemia in obese type 2 diabetic rats; these effects were associated with increased EPO and HIF-1 levels in kidney and liver as well as increased circulating EPO, testosterone, and iron. (Source: Nitric Oxide)
Source: Nitric Oxide - February 14, 2019 Category: Chemistry Source Type: research

Involvement of RhoA/Rho-kinase in l-cysteine/H2S pathway-induced inhibition of agonist-mediated corpus cavernosal smooth muscle contraction
Publication date: Available online 13 February 2019Source: Nitric OxideAuthor(s): Fatma Aydinoglu, Elif Özveren Adıbelli, Didem Yılmaz-Oral, Nuran OgulenerAbstractRho-kinase activity is a key regulator in the maintenance of corporal vasoconstriction and penile detumescense. Also, importance of l-cysteine/H2S pathway in erectile tissue has been shown; however it is currently unknown the role RhoA/Rho-kinase pathway in H2S-induced inhibition in cavernosal tissue. We investigated the role of RhoA/Rho-kinase pathway in the inhibitory effect of l-cysteine and NaHS, as endogenous and exogenous H2S, respectively, on phenyl...
Source: Nitric Oxide - February 13, 2019 Category: Chemistry Source Type: research

Controlled light-induced gas phase nitric oxide release from S-nitrosothiol-doped silicone rubber films
Publication date: Available online 5 February 2019Source: Nitric OxideAuthor(s): Gergely Lautner, Blake Stringer, Elizabeth J. Brisbois, Mark E. Meyerhoff, Steven P. SchwendemanAbstractThe light induced nitric oxide (NO) release properties of S-nitroso-N-acetylpenicillamine (SNAP) and S-nitrosoglutathione (GSNO) NO donors doped within polydimethylsiloxane (PDMS) films (PDMS-SNAP and PDMS-GSNO respectively) for potential inhaled NO (iNO) applications is examined. To achieve photolytic release of gas phase NO from the PDMS-SNAP and PDMS-GSNO films, narrow band LED light sources are employed and the NO concentration in a N2 s...
Source: Nitric Oxide - February 6, 2019 Category: Chemistry Source Type: research

Mechanisms impairing blood pressure responses to nitrite and nitrate
Publication date: Available online 1 February 2019Source: Nitric OxideAuthor(s): Gustavo H. Oliveira-Paula, Lucas C. Pinheiro, Jose E. Tanus-SantosAbstractHypertension is a multifactorial disease associated with impaired nitric oxide (NO) production and bioavailability. In this respect, restoring NO activity by using nitrite and nitrate has been considered a potential therapeutic strategy to treat hypertension. This possibility is justified by the understanding that both nitrite and nitrate may be recycled back to NO and also promote the generation of other bioactive species. This process involves a complex biological circ...
Source: Nitric Oxide - February 2, 2019 Category: Chemistry Source Type: research

Acceleration of the autoxidation of nitric oxide by proteins
Publication date: Available online 30 January 2019Source: Nitric OxideAuthor(s): Matías N. Möller, Ana DenicolaAbstractLipoproteins and lipid membranes accelerate •NO autoxidation by increasing local concentration of •NO and O2. Although the idea that proteins could also accelerate this reaction was presented some time ago, it was largely criticized and dismissed. Herein the effect of proteins on •NO autoxidation rates was studied following •NO disappearance with a selective electrode. It was found that human serum albumin (HSA) accelerated •NO autoxidation by a factor of 9 per g/mL of ...
Source: Nitric Oxide - January 31, 2019 Category: Chemistry Source Type: research

Editorial Board
Publication date: 1 March 2019Source: Nitric Oxide, Volume 84Author(s): (Source: Nitric Oxide)
Source: Nitric Oxide - January 30, 2019 Category: Chemistry Source Type: research

The era of nitric oxide in plant biology: Twenty years tying up loose ends
Publication date: Available online 28 January 2019Source: Nitric OxideAuthor(s): Fiorella Del Castello, Andrés Nejamkin, Raul Cassia, Natalia Correa-Aragunde, Belén Fernández, Noelia Foresi, Cristina Lombardo, Leonor Ramirez, Lorenzo LamattinaAbstractNitric oxide (NO) is an essential signal molecule to maintain cellular homeostasis in uni and pluricellular organisms. Conceptually, NO intervenes as much in sustaining basal metabolic processes, as in firing cellular responses to changes in internal and external conditions, and also in guiding the return to basal conditions. Behind these unusual capabilit...
Source: Nitric Oxide - January 28, 2019 Category: Chemistry Source Type: research

Chronic high-dose beetroot juice supplementation improves time trial performance of well-trained cyclists in normoxia and hypoxia
Publication date: Available online 24 January 2019Source: Nitric OxideAuthor(s): Torben Rokkedal-Lausch, Jesper Franch, Mathias K. Poulsen, Lars P. Thomsen, Eddie Weitzberg, Ernest N. Kamavuako, Dan S. Karbing, Ryan G. LarsenAbstractDietary nitrate (NO3−) supplementation via beetroot juice (BR) is known to improve endurance performance in untrained and moderately trained individuals. However, conflicting results exist in well-trained individuals. Evidence suggests that the effects of NO3− are augmented during conditions of reduced oxygen availability (e.g., hypoxia), thereby increasing the probability of perfor...
Source: Nitric Oxide - January 24, 2019 Category: Chemistry Source Type: research

Effects of acute dietary nitrate supplementation on aortic blood pressures and pulse wave characteristics in post-menopausal women
Publication date: Available online 19 January 2019Source: Nitric OxideAuthor(s): Danielle Jin-Kwang Kim, Carly A. Roe, Yasina Somani, David J. Moore, Megan A. Barrett, Michael Flanagan, Daniel B. Kim-Shapiro, Swati Basu, Matthew D. Muller, David N. ProctorAbstractPurposeConsumption of nitrate-rich beetroot juice can lower blood pressure in peripheral as well as central arteries and may exert additional hemodynamic benefits (e.g. reduced aortic wave reflections). The specific influence of nitrate supplementation on arterial pressures and aortic wave properties in postmenopausal women, a group that experiences accelerated in...
Source: Nitric Oxide - January 20, 2019 Category: Chemistry Source Type: research

Editorial for Special Issue: “Pharmacology & Medicinal Chemistry of Nitric Oxide”
Publication date: Available online 16 January 2019Source: Nitric OxideAuthor(s): (Source: Nitric Oxide)
Source: Nitric Oxide - January 17, 2019 Category: Chemistry Source Type: research

Dexmedetomidine protects against lipopolysaccharide-induced early acute kidney injury by inhibiting the iNOS/NO signaling pathway in rats
Publication date: Available online 17 January 2019Source: Nitric OxideAuthor(s): Yongping Chen, Li Luan, Chaoran Wang, Manyu Song, Yuan Zhao, Yujie Yao, Haotian Yang, Biao Ma, Honggang FanAbstractIncreasing evidence has demonstrated that dexmedetomidine (DEX) possesses multiple pharmacological actions. Herein, we explored the protective effect and potential molecular mechanism of DEX on lipopolysaccharide (LPS)-induced early acute kidney injury (AKI) from the perspective of antioxidant stress. We found that DEX (30 μg/kg, i.p.) ameliorated the renal dysfunction and histopathological damage (tubular necrosis, vacuolar ...
Source: Nitric Oxide - January 17, 2019 Category: Chemistry Source Type: research

Encapsulation of S-nitrosoglutathione into chitosan nanoparticles improves drought tolerance of sugarcane plants
Publication date: 1 March 2019Source: Nitric Oxide, Volume 84Author(s): Neidiquele M. Silveira, Amedea B. Seabra, Fernanda C.C. Marcos, Milena T. Pelegrino, Eduardo C. Machado, Rafael V. RibeiroAbstractThe entrapment of NO donors in nanomaterials has emerged as a strategy to protect these molecules from rapid degradation, allowing a more controlled release of NO and prolonging its effect. On the other hand, we have found beneficial effects of S-nitrosoglutathione (GSNO) – a NO donor – supplying to sugarcane plants under water deficit. Here, we hypothesized that GSNO encapsulated into nanoparticles would be more...
Source: Nitric Oxide - January 15, 2019 Category: Chemistry Source Type: research