Decreased expression of neuronal nitric oxide synthase contributes to the endothelial dysfunction associated with cigarette smoking in human
Publication date: Available online 25 February 2020Source: Nitric OxideAuthor(s): Eduardo D. Costa, Josiane F. Silva, Daniela C. Garcia, Alberto J. Wainstein, Bruno A. Rezende, Rita C. Tostes, Mauro M. Teixeira, Steyner F. Cortes, Virginia S. Lemos (Source: Nitric Oxide)
Source: Nitric Oxide - February 26, 2020 Category: Chemistry Source Type: research

Recent developments of fluorescent probes for detection and bioimaging of nitric oxide
Publication date: Available online 21 February 2020Source: Nitric OxideAuthor(s): Yi ChenAbstractNitric oxide (NO) is an important signaling molecule implicated in numerous physiological and pathological conditions. NO homeostasis is crucial to its proper functions and misregulation of NO production is implicated in a large number of pathologies. The effect of NO depends on the cell microenvironment as well as its concentration, spatial, and temporal constraints. Thus, the visualization and monitoring of NO in living systems would be valuable for understanding the origin, activities, and biological effects of NO. This revi...
Source: Nitric Oxide - February 23, 2020 Category: Chemistry Source Type: research

Editorial Board
Publication date: 1 April 2020Source: Nitric Oxide, Volume 97Author(s): (Source: Nitric Oxide)
Source: Nitric Oxide - February 21, 2020 Category: Chemistry Source Type: research

Nitroxides affect neurological deficits and lesion size induced by a rat model of traumatic brain injury
In conclusion, DCTEIO significantly improved tissue repair and preserved neurological function in rats with TBI possibly via a mitophagy mechanism. This study provides evidence for DCTEIO as a promising new option to alleviate lesion severity after moderate TBI, which is not actively treated. (Source: Nitric Oxide)
Source: Nitric Oxide - February 14, 2020 Category: Chemistry Source Type: research

Interactive role of exogenous 24 Epibrassinolide and endogenous NO in Brassica juncea L. under salinity stress: Evidence for NR-dependent NO biosynthesis
Publication date: Available online 8 February 2020Source: Nitric OxideAuthor(s): Praveen Gupta, Chandra Shekhar SethAbstractThe present study unravels origin of nitric oxide (NO) and the interaction between 24-Epibrassinolide (EBL) and nitrate reductase (NR) for NO production in Indian mustard (Brassica juncea L.) under salinity stress. Two independent experiments were performed to check whether (i) Nitrate reductase or Nitric oxide synthase takes part in the biosynthesis of endogenous NO and (ii) EBL has any regulatory effect on NR-dependent NO biosynthesis in the alleviation of salinity stress. Results revealed that NR-i...
Source: Nitric Oxide - February 10, 2020 Category: Chemistry Source Type: research

Head-to-head comparison of inorganic nitrate and metformin in a mouse model of cardiometabolic disease
Conclusion/ImplicationsNitrate and metformin had equipotent metabolic effects, while nitrate was superior regarding protection against cardiovascular dysfunction and liver steatosis. If reproduced in future clinical trials, these findings may have implications for novel nutrition-based strategies against metabolic syndrome, T2D and associated complications.Graphical abstract (Source: Nitric Oxide)
Source: Nitric Oxide - February 5, 2020 Category: Chemistry Source Type: research

Neonatal comorbidities and gasotransmitters
Publication date: Available online 31 January 2020Source: Nitric OxideAuthor(s): Samuel J. Gentle, Trent E. Tipple, Rakesh PatelAbstractHydrogen sulfide, nitric oxide, and carbon monoxide are endogenously produced gases that regulate various signaling pathways. The role of these transmitters is complex as constitutive production of these molecules may have anti-inflammatory, anti-microbial, and/or vasodilatory effects whereas induced production or formation of secondary metabolites may lead to cellular death. Given this fine line between friend and foe, therapeutic attenuation of these molecules’ production has invol...
Source: Nitric Oxide - February 1, 2020 Category: Chemistry Source Type: research

Renal nitrate clearance in chronic kidney disease
Publication date: Available online 30 January 2020Source: Nitric OxideAuthor(s): J.K. Williams, M.J. Smallwood, N. Benjamin, R.J. D'Souza, A.C. Shore, P.G. Winyard, M. GilchristAbstractBackgroundNitric oxide (NO) is rapidly oxidised in humans to nitrite and nitrate, with nitrate being present in much greater abundance. These oxidation products can be recycled back into nitric oxide via a complex entero-salivary pathway, thus preserving NO activity. Approximately 65% of circulating nitrate is excreted in the urine in 48 h, with the excretory pathway of the remainder unknown. The effect of declining renal function on nitra...
Source: Nitric Oxide - February 1, 2020 Category: Chemistry Source Type: research

A comparison of multifunctional donors of carbon monoxide: Their anticoagulant, antioxidant, anti-aggregatory and cytotoxicity activities in an in vitro model
Publication date: Available online 30 January 2020Source: Nitric OxideAuthor(s): Weronika Adach, Beata OlasAbstractThe study examines the effect of two water-soluble carbon monoxide (CO) donors, CORM-3 and CORM-A1, on selected parameters of oxidative stress and hemostasis in human plasma and blood platelets in vitro. It also compares their activity with that of the lipid-soluble CORM-2. The oxidation of amino acid residues in plasma proteins was evaluated by measuring the amounts of thiol and carbonyl groups. Plasma lipid peroxidation was measured as thiobarbituric acid reactive substance (TBARS) concentration. In addition...
Source: Nitric Oxide - January 30, 2020 Category: Chemistry Source Type: research

Editorial Board
Publication date: 1 March 2020Source: Nitric Oxide, Volume 96Author(s): (Source: Nitric Oxide)
Source: Nitric Oxide - January 29, 2020 Category: Chemistry Source Type: research

Intratracheal injection of nitric oxide, generated from air by pulsed electrical discharge, for the treatment of pulmonary hypertension in awake ambulatory lambs
ConclusionsContinuously delivering electrically generated NO through a Scoop catheter produces vasodilation of the pulmonary vasculature of awake lambs with pulmonary hypertension. Transtracheal NO delivery may provide a long-term treatment for patients with chronic pulmonary hypertension as an outpatient without requiring a mask or tracheal intubation. (Source: Nitric Oxide)
Source: Nitric Oxide - January 24, 2020 Category: Chemistry Source Type: research

Poor glycemic control impairs the cardioprotective effects of red blood cells on myocardial ischemia/reperfusion injury
Publication date: Available online 22 January 2020Source: Nitric OxideAuthor(s): Johanna M. Muessig, Luise Moellhoff, Johanna Noelle, Sema Kaya, Leonie Hidalgo Pareja, Maryna Masyuk, Michael Roden, Malte Kelm, Christian JungAbstractRed blood cells (RBCs) play an important role in the cardiac ischemia/reperfusion (I/R) injury. Cardiovascular risk factors impair the RBC function in an endothelial nitric oxide synthase (eNOS) dependent manner. However, it is unclear whether the protective role of RBCs can be rescued by modifying cardiovascular risk factors or by pharmacologic intervention. RBCs obtained from elderly patients ...
Source: Nitric Oxide - January 23, 2020 Category: Chemistry Source Type: research

Supplementation of dietary nitrate attenuated oxidative stress and endothelial dysfunction in diabetic vasculature through inhibition of NADPH oxidase
Publication date: Available online 21 January 2020Source: Nitric OxideAuthor(s): Rong Tian, Rou Peng, Ziyi Yang, Yi-Yuan Peng, Naihao LuAbstractThe metabolic disorders in diabetes, which are usually accompanied by oxidative stress and impaired nitric oxide (NO) bioavailability, increase the risk of detrimental cardiovascular complications. Herein, we investigated the therapeutic potential of dietary nitrate, which is found in high content in green leafy vegetables, on vascular oxidative stress and endothelial dysfunction in diabetic mice induced by high-fat diet and streptozotocin injection. Dietary nitrate in drinking wat...
Source: Nitric Oxide - January 21, 2020 Category: Chemistry Source Type: research

Mechanisms of the protective effects of nitrate and nitrite in cardiovascular and metabolic diseases
Publication date: Available online 16 January 2020Source: Nitric OxideAuthor(s): Yang Liu, Kevin D. Croft, Jonathan M. Hodgson, Trevor Mori, Natalie C. WardAbstractWithin the body, NO is produced by nitric oxide synthases via converting l-arginine to citrulline. Additionally, NO is also produced via the NOS-independent nitrate-nitrite-NO pathway. Unlike the classical pathway, the nitrate-nitrite-NO pathway is oxygen independent and viewed as a back-up function to ensure NO generation during ischaemia/hypoxia. Dietary nitrate and nitrite have emerged as substrates for endogenous NO generation and other bioactive nitrogen ox...
Source: Nitric Oxide - January 17, 2020 Category: Chemistry Source Type: research

Real-time redox monitoring of a nitrosyl ruthenium complex acting as NO-donor agent in a single A549 cancer cell with multiplex Fourier-transform infrared microscopy
Publication date: Available online 14 January 2020Source: Nitric OxideAuthor(s): Fernando Postalli Rodrigues, Lucyano J.A. Macedo, Leandro N.C. Máximo, Fernanda C.P.F. Sales, Roberto Santana da Silva, Frank N. CrespilhoAbstractMultiplex Fourier-transform infrared microscopy (μFT-IR) helped to monitor trans-[Ru(NO) (NH3)4 (isn)]3+ (I), uptake by A549 lung carcinoma cell, as well as the generation of its product, nitric oxide (NO), inside the cell. Chronoamperometry with NO-sensor and μFT-IR showed that exogenous NADH and the A549 cell induced the NO release redox mechanism. Chemical imaging confirmed that (I)...
Source: Nitric Oxide - January 15, 2020 Category: Chemistry Source Type: research

Inhaled nitric oxide to control platelet hyper-reactivity in patients with acute submassive pulmonary embolism
Publication date: Available online 12 January 2020Source: Nitric OxideAuthor(s): Jeffrey A. Kline, Michael A. Puskarich, Jonathan Pike, John Zagorski, Nathan J. AlvesAbstractBackgroundWe test if inhaled nitric oxide (NO) attenuates platelet functional and metabolic hyper-reactivity in subjects with submassive pulmonary embolism (PE).MethodsParticipants with PE were randomized to either 50 ppm NO + O2 or O2 only for 24 h with blood sampling at enrollment and after treatment; results were compared with healthy controls. Platelet metabolic activity was assessed by oxygen consumption (basal and uncoupled) a...
Source: Nitric Oxide - January 12, 2020 Category: Chemistry Source Type: research

Nitric oxide synthase inhibitor L-NG-nitroarginine methyl ester (L-NAME) attenuates remobilization-induced joint inflammation
This study aimed to investigate whether: 1) administration of the NOS inhibitor L-NG-nitroarginine methyl ester (l-NAME) can attenuate remobilization-induced joint inflammation; and 2) hypoxia/reoxygenation is induced by joint immobilization and followed by remobilization. Unilateral knee joints of rats were immobilized using external fixators for three weeks. After removal of the fixation device, knees were allowed to move freely for one day (remobilization) with or without l-NAME administration. Without L-NAME administration, inflammatory reactions including joint swelling and inflammatory cell infiltration, edema, and u...
Source: Nitric Oxide - January 9, 2020 Category: Chemistry Source Type: research

Editorial Board
Publication date: 1 February 2020Source: Nitric Oxide, Volume 95Author(s): (Source: Nitric Oxide)
Source: Nitric Oxide - January 8, 2020 Category: Chemistry Source Type: research

Maternal disease and gasotransmitters
Publication date: Available online 3 January 2020Source: Nitric OxideAuthor(s): Aishwarya Rengarajan, Amanda K. Mauro, Derek S. BoeldtAbstractThe three known gasotransmitters, nitric oxide, carbon monoxide, and hydrogen sulfide are involved in key processes throughout pregnancy. Gasotransmitters are known to impact on smooth muscle tone, regulation of immune responses, and oxidative state of cells and their component molecules. Failure of the systems that tightly regulate gasotransmitter production and downstream effects are thought to contribute to common maternal diseases such as preeclampsia and preterm birth. Normal pr...
Source: Nitric Oxide - January 5, 2020 Category: Chemistry Source Type: research

Cellular microdomains for nitric oxide signaling in endothelium and red blood cells
Publication date: Available online 3 January 2020Source: Nitric OxideAuthor(s): Francesca Leo, Beate Hutzler, Claire A. Ruddiman, Brant E. Isakson, Miriam M. Cortese-KrottAbstractThere is accumulating evidence that biological membranes are not just homogenous lipid structures, but are highly organized in microdomains, i.e. compartmentalized areas of protein and lipid complexes, which facilitate necessary interactions for various signaling pathways. Each microdomain exhibits unique composition, membrane location, and dynamics that ultimately shape their functional characteristics. In the vasculature, microdomains are crucia...
Source: Nitric Oxide - January 5, 2020 Category: Chemistry Source Type: research

The role of gasotransmitters in neonatal physiology
Publication date: Available online 20 December 2019Source: Nitric OxideAuthor(s): Taiming Liu, George T. Mukosera, Arlin B. BloodAbstractThe gasotransmitters, nitric oxide (NO), hydrogen sulfide (H2S), and carbon monoxide (CO), are endogenously-produced volatile molecules that perform signaling functions throughout the body. In biological tissues, these small, lipid-permeable molecules exist in free gaseous form for only seconds or less, and thus they are ideal for paracrine signaling that can be controlled rapidly by changes in their rates of production or consumption. In addition, tissue concentrations of the gasotransmi...
Source: Nitric Oxide - December 21, 2019 Category: Chemistry Source Type: research

DDAH-1 via HIF-1 target genes improves cerebral ischemic tolerance after hypoxic preconditioning and middle cerebral artery occlusion-reperfusion
Publication date: Available online 20 December 2019Source: Nitric OxideAuthor(s): Yichen Zhao, Yuchen Zhou, Xiaoye Ma, Xiaohui Liu, Yanxin Zhao, Xueyuan LiuAbstractDimethylarginine dimethylamino hydrolase-1 (DDAH-1) as an indirect regulator of nitric oxide (NO) metabolism, its role in hypoxic preconditioning (HPC) and ischemic tolerance (IT) of ischemic stroke has still been unknown and needs to be elucidated. Herein, DDAH-1 knock-out (KO) and wild-type (WT) rats underwent HPC and middle cerebral artery occlusion/reperfusion (MCAO/R) model. After 24 h, neurological severity scores, TTC staining and TUNEL assay were used ...
Source: Nitric Oxide - December 21, 2019 Category: Chemistry Source Type: research

Therapies that enhance pulmonary vascular NO-signaling in the neonate
Publication date: Available online 20 December 2019Source: Nitric OxideAuthor(s): Julie Dillard, Marta Perez, Bernadette ChenAbstractThere are several pulmonary hypertensive diseases that affect the neonatal population, including persistent pulmonary hypertension of the newborn (PPHN) and bronchopulmonary dysplasia (BPD)-associated pulmonary hypertension (PH). While the indication for inhaled nitric oxide (iNO) use is for late-preterm and term neonates with PPHN, there is a suboptimal response to this pulmonary vasodilator in ∼40% of patients. Additionally, there are no FDA-approved treatments for BPD-associated PH or ...
Source: Nitric Oxide - December 21, 2019 Category: Chemistry Source Type: research

Inhaled nitric oxide use in neonates: Balancing what is evidence-based and what is physiologically sound
This article reviews the off-label uses of iNO in infants. Rationale is discussed for a selective application of iNO based on physiologically guided principles, and new research avenues are considered. (Source: Nitric Oxide)
Source: Nitric Oxide - December 20, 2019 Category: Chemistry Source Type: research

Nitric oxide signaling in pregnancy and preeclampsia
Publication date: Available online 15 December 2019Source: Nitric OxideAuthor(s): Elizabeth F. Sutton, Mary Gemmel, Robert W. PowersAbstractWhile the etiology of preeclampsia continues to be elucidated, it is clear that preeclampsia is a complex obstetrical syndrome associated with maternal vascular dysfunction within which impairments in nitric oxide (NO) signaling likely play a key role in driving disease progression and severity. The goal of this review is to present the available evidence for maladaptations in NO and NO signaling in pregnancies complicated by preeclampsia. After a brief overview of preeclampsia, a revi...
Source: Nitric Oxide - December 17, 2019 Category: Chemistry Source Type: research

Editorial Board
Publication date: 1 January 2020Source: Nitric Oxide, Volume 94Author(s): (Source: Nitric Oxide)
Source: Nitric Oxide - December 11, 2019 Category: Chemistry Source Type: research

Saliva panel of protein candidates: A comprehensive study for assessing high altitude acclimatization
In this study, we employed iTRAQ based LC-MS/MS for comparing saliva samples from humans exposed to hypobaric hypoxia from 7 to 120 days with normoxic controls followed by analysis using Ingenuity Pathway Analysis software and validation by immunoassays. Nearly 67 proteins were found to be differentially expressed in the exposed groups as compared to normoxia indicating modulated canonical pathways as lipid metabolism; acute phase response signalling and proteins as carbonic anhydrase 6, alpha-enolase, albumin, and prolactin inducible protein. Collectively, this study provides the proof of concept for the non-invasive asse...
Source: Nitric Oxide - November 26, 2019 Category: Chemistry Source Type: research

Nitric oxide signaling inhibits microglia proliferation by activation of protein kinase-G
Publication date: Available online 21 November 2019Source: Nitric OxideAuthor(s): Matthew J.E. Maksoud, Vasiliki Tellios, Yun-Yan Xiang, Wei-Yang LuAbstractMicroglia population is primarily determined by a finely-regulated proliferation process during early development of the central nervous system (CNS). Nitric oxide (NO) is known to inhibit proliferation of numerous types of cells. However, how NO signaling regulates microglia proliferation remains elusive. Using wildtype (WT) and inducible nitric oxide synthase knockout (iNOS−/-) mice, this study investigated the role and underlying mechanisms of iNOS/NO signaling...
Source: Nitric Oxide - November 22, 2019 Category: Chemistry Source Type: research

Delivering nitric oxide into human skin from encapsulated S-nitrosoglutathione under UV light: An in vitro and ex vivo study
Publication date: Available online 21 November 2019Source: Nitric OxideAuthor(s): Milena T. Pelegrino, Richard B. Weller, André Paganotti, Amedea B. SeabraAbstractNitric oxide (NO) is a crucial molecule in the human body. The encapsulation of exogenous NO donors into chitosan nanoparticles (CS NPs) has been widely used to overcome NO drawbacks in pharmacological applications, such as, its short half-life. The NO donor, S-nitrosoglutathione (GSNO), was encapsulated into CS NPs (GSNO-CS NPs) and characterized by AFM and DLS measurements. The nanoparticles presented a hydrodynamic size of 123.3 ± 1.5 nm an...
Source: Nitric Oxide - November 21, 2019 Category: Chemistry Source Type: research

Role of hippocampal nitrergic neurotransmission in behavioral and cardiovascular dysfunctions evoked by chronic social stress
Publication date: Available online 21 November 2019Source: Nitric OxideAuthor(s): Jeferson Almeida, Leandro A. Oliveira, Ricardo Benini, Carlos C. CrestaniAbstractIncreased nitric oxide (NO) levels have been identified in the hippocampus of animals subjected to social isolation. However, a role of this change in behavioral and physiological changes evoked by isolation has never been evaluated. Thus, this study investigated the involvement of nitrergic neurotransmission acting via the neuronal isoform of nitric oxide synthase (nNOS) within the dorsal hippocampus in behavioral and cardiovascular changes in isolated reared ra...
Source: Nitric Oxide - November 21, 2019 Category: Chemistry Source Type: research

Exogenous nitric oxide alleviates sulfur deficiency-induced oxidative damage in tomato seedlings
Publication date: Available online 7 November 2019Source: Nitric OxideAuthor(s): Manzer H. Siddiqui, Saud Alamri, Qasi D. Alsubaie, Hayssam M. Ali, M. Nasir Khan, Abdullah Al-Ghamdi, Abdullah A. Ibrahim, Abdullah AlsadonAbstractDespite numerous reports on the role of nitric oxide (NO) in regulating plants growth and mitigating different environmental stresses, its participation in sulfur (S) -metabolism remains largely unknown. Therefore, we studied the role of NO in S acquisition and S-assimilation in tomato seedlings under low S-stress conditions by supplying NO to the leaves of S-sufficient and S-deficient seedlings. S-...
Source: Nitric Oxide - November 9, 2019 Category: Chemistry Source Type: research

Past, current and future perspectives of the use of inhaled NO as a primary therapeutic for the newborn
Publication date: Available online 6 November 2019Source: Nitric OxideAuthor(s): Joseph B. Philips (Source: Nitric Oxide)
Source: Nitric Oxide - November 7, 2019 Category: Chemistry Source Type: research

Editorial Board
Publication date: 1 December 2019Source: Nitric Oxide, Volume 93Author(s): (Source: Nitric Oxide)
Source: Nitric Oxide - November 6, 2019 Category: Chemistry Source Type: research

Sickle cell disease subjects and mouse models have elevated nitrite and cGMP levels in blood compartments
Publication date: Available online 2 November 2019Source: Nitric OxideAuthor(s): Luis E.F. Almeida, Sayuri Kamimura, Celia M. de Souza Batista, Nicholas Spornick, Margaret Y. Nettleton, Elizabeth Walek, Meghann L. Smith, Julia Finkel, Deepika Darbari, Paul Wakim, Zenaide M.N. QuezadoAbstractThe hypothesis of decreased nitric oxide (NO) bioavailability in sickle cell disease (SCD) proposes that multiple factors leading to decreased NO production and increased consumption contributes to vaso-occlusion, pulmonary hypertension, and pain. The anion nitrite is central to NO physiology as it is an end product of NO metabolism and...
Source: Nitric Oxide - November 3, 2019 Category: Chemistry Source Type: research

Effects of age, sex and diet on salivary nitrate and nitrite in infants
Publication date: Available online 2 November 2019Source: Nitric OxideAuthor(s): Niklas Timby, Magnus Domellöf, Olle Hernell, Bo Lönnerdal, Carina Nihlen, Ingegerd Johanssson, Eddie WeitzbergAbstractThe inorganic anions nitrate and nitrite are oxidation products from endogenous nitric oxide (NO) generation and constituents in our diet. A nitrate-nitrite-NO pathway exists in which nitrate can be serially reduced to bioactive NO. The first step of this pathway occurs in the oral cavity where oral bacteria convert salivary nitrate to nitrite, whereafter nitrite is reduced to NO systemically by several enzymatic and ...
Source: Nitric Oxide - November 3, 2019 Category: Chemistry Source Type: research

Influence of hemoglobin and albumin on the NO donation effect of tetranitrosyl iron complex with thiosulfate
Publication date: Available online 31 October 2019Source: Nitric OxideAuthor(s): Olesya Pokidova, Tatiana Rudneva, Bogdan Tretyakov, Raisa Kotelnikova, Alexander Kotelnikov, Sergey AldoshinAbstractThe effects of deoxyhemoglobin (Hb) and albumin on the NO-donor activity of the anionic tetranitrosyl iron complex with thiosulfate ligands (1) were studied for the first time. It was shown that Hb significantly stabilizes complex 1; in its presence, NO generation from the complex proceeds at a noticeably slower rate. A similar effect is observed when complex 1 is bound to albumin, in which case complex 1 decomposes 27 times slow...
Source: Nitric Oxide - November 1, 2019 Category: Chemistry Source Type: research

A randomized pilot study of nitrate supplementation with beetroot juice in acute respiratory failure
Publication date: Available online 25 October 2019Source: Nitric OxideAuthor(s): D. Clark Files, Timothy Heinrich, Katherine L. Shields, Nathan J. Love, Carly Brailer, Rita N. Bakhru, Lina Purcell, Lori Flores, Kevin Gibbs, Gary D. Miller, Peter E. Morris, Michael J. BerryAbstractNitrate rich beetroot juice (BRJ) can enhance nitric oxide signaling, leading to improved physical function in healthy and diseased populations, but its safety and biologic efficacy have not been evaluated in a critically ill population. We randomized 22 previously functional acute respiratory failure patients to either BRJ or placebo daily until ...
Source: Nitric Oxide - October 26, 2019 Category: Chemistry Source Type: research

Modulation of endothelium-derived nitric oxide production and activity by taurine and taurine-conjugated bile acids
Publication date: Available online 24 October 2019Source: Nitric OxideAuthor(s): Daniele M. Guizoni, Jean F. Vettorazzi, Everardo M. Carneiro, Ana Paula DavelAbstractTaurine is a semiessential amino acid found at high concentrations in mammalian plasma and cells, where it regulates cellular functions such as ion flux, controls cell volume and serves as a substrate for conjugated bile acids (BAs). Exogenous administration of both taurine and taurine-conjugated BAs have also been implicated in the modulation of cardiovascular functions. This brief review summarizes the role of taurine and taurine-conjugated BAs in vascular r...
Source: Nitric Oxide - October 25, 2019 Category: Chemistry Source Type: research

Central angiotensin II-Protein inhibitor of neuronal nitric oxide synthase (PIN) axis contribute to neurogenic hypertension
Publication date: Available online 22 October 2019Source: Nitric OxideAuthor(s): Neeru M. Sharma, Andrea S. Haibara, Kenichi Katsurada, Xuefei Liu, Kaushik P. PatelAbstractActivation of renin-angiotensin- system, nitric oxide (NO•) bioavailability and subsequent sympathoexcitation plays a pivotal role in the pathogenesis of many cardiovascular diseases, including hypertension. Previously we have shown increased protein expression of PIN (a protein inhibitor of nNOS: neuronal nitric oxide synthase, known to dissociate nNOS dimers into monomers) with concomitantly reduced levels of catalytically active dimers of nNOS in...
Source: Nitric Oxide - October 23, 2019 Category: Chemistry Source Type: research

Treatment with nitrite prevents reactive oxygen species generation in the corpora cavernosa and restores intracavernosal pressure in hypertensive rats
Publication date: Available online 11 October 2019Source: Nitric OxideAuthor(s): Natália A. Gonzaga, Gabriel T. do Vale, Carla B.P. da Silva, Lucas C. Pinheiro, Letícia N. Leite, Fernando S. Carneiro, José E. Tanus-Santos, Carlos R. TirapelliAbstractHypertension is a risk factor for erectile dysfunction (ED) and both conditions are associated with oxidative stress. Given that nitrite is described to display antioxidant effects, we hypothesized that treatment with nitrite would exert antioxidant effects attenuating both reactive oxygen species (ROS) generation in the corpora cavernosa (CC) and ED induce...
Source: Nitric Oxide - October 13, 2019 Category: Chemistry Source Type: research

Propargylglycine decreases neuro-immune interaction inducing pain response in temporomandibular joint inflammation model
Publication date: Available online 8 October 2019Source: Nitric OxideAuthor(s): Emanuela G. Garattini, Bruna M. Santos, Daniele Ferrari, Camila Porto, Heloísa D.C. Francescato, Terezila M. Coimbra, Christie R.A. Leite-Panissi, Luiz G.S. Branco, Glauce C. NascimentoAbstractThe mechanisms underlying temporomandibular disorders following orofacial pain remain unclear. Hydrogen sulfide (H2S), a newly identified gasotransmitter, has been reported to modulate inflammation. Cystathionine γ-lyase (CSE) is responsible for the systemical production of H2S, which exerts both pro- and antinociceptive effects through infla...
Source: Nitric Oxide - October 10, 2019 Category: Chemistry Source Type: research

Sapropterin reduces coronary artery malformation in offspring of pregestational diabetes mice
In conclusion, sapropterin treatment increases eNOS activity, reduces oxidative stress and prevents coronary artery malformation in offspring of pregestational diabetes. Sapropterin may have therapeutic potential in preventing coronary artery malformation in maternal diabetes.Graphical abstractOral sapropterin (Kuvan®) treatment inhibits oxidative stress, improves Akt/eNOS activity, and prevents coronary artery malformation (CAM) during pregestational diabetes. (Source: Nitric Oxide)
Source: Nitric Oxide - October 9, 2019 Category: Chemistry Source Type: research

Effects of dietary nitrate supplementation on microvascular physiology at 4559 m altitude – A randomised controlled trial (Xtreme Alps)
Publication date: Available online 8 October 2019Source: Nitric OxideAuthor(s): Andrew F. Cumpstey, Philip J. Hennis, Edward T. Gilbert-Kawai, Bernadette O. Fernandez, Daniel Grant, William Jenner, Matthieu Poudevigne, Helen Moyses, Denny ZH. Levett, Alexandra Cobb, Paula Meale, Kay Mitchell, Helmut Pöhnl, Monty G. Mythen, Michael PW. Grocott, Daniel S. Martin, Martin Feelisch, Tom Adams, Lindsay Bisiker, Adam BoothAbstractNative highlanders (e.g. Sherpa) demonstrate remarkable hypoxic tolerance, possibly secondary to higher levels of circulating nitric oxide (NO) and increased microcirculatory blood flow. As part of ...
Source: Nitric Oxide - October 9, 2019 Category: Chemistry Source Type: research

Nitrate uptake and metabolism in human skeletal muscle cell cultures
Publication date: Available online 8 October 2019Source: Nitric OxideAuthor(s): Sirada Srihirun, Ji Won Park, Rujia Teng, Waritta Sawaengdee, Barbora Piknova, Alan N. SchechterAbstractSeveral studies show that dietary nitrate enhances exercise performance, presumably by increasing muscle blood flow and improving oxygen utilization. These effects are likely mediated by nitrate metabolites, including nitrite and nitric oxide (NO). However, the mechanisms of nitrate production, storage, and metabolism to nitrite and NO in skeletal muscle cells are still unclear. We hypothesized that exogenous nitrate can be taken up and metab...
Source: Nitric Oxide - October 9, 2019 Category: Chemistry Source Type: research

Systemic vasoprotection by inhaled carbon monoxide is mediated through prolonged alterations in monocyte/macrophage function
Publication date: Available online 5 October 2019Source: Nitric OxideAuthor(s): Andrew Leake, Karim Salem, Michael C. Madigan, Ghee Rye Lee, Ankur Shukla, Guiying Hong, Brian S. Zuckerbraun, Edith TzengAbstractCarbon monoxide (CO) is anti-inflammatory and protective in models of disease. Its actions in vitro are short-lived but are sustained in vivo. We hypothesize that systemic CO can mediate prolonged phenotype changes in vivo, with a focus on macrophages (Mϕs). Mϕs isolated from CO treated rats responded to lipopolysaccharide (LPS) with increased IL6, IL10 and iNOS expression but decreased TNF. Conditioned media (CM) ...
Source: Nitric Oxide - October 6, 2019 Category: Chemistry Source Type: research

Inhibition of breast tumor growth by N(G)-nitro-l-arginine methyl ester (l-NAME) is accompanied by activation of fibroblasts
Publication date: Available online 19 September 2019Source: Nitric OxideAuthor(s): Marianela Sciacca, Denise Belgorosky, Macarena Zambrano, José Ignacio Gomez Escalante, Fernanda Roca, Yanina V. Langle, Eduardo O. Sandes, Catalina Lodillinsky, Ana María EijánAbstractNitric Oxide (NO) is involved in many physiological and pathological processes. It is generated by a family of NO synthases (NOS), being the inducible isoform, iNOS, responsible for higher amounts of NO. Here, we report that pharmacological inhibition of NO production by l-NAME reduces both viability and MAPK activated signalling pathways i...
Source: Nitric Oxide - September 21, 2019 Category: Chemistry Source Type: research

A forty year journey: The generation and roles of NO in plants
Publication date: Available online 18 September 2019Source: Nitric OxideAuthor(s): Zs Kolbert, J.B. Barroso, R. Brouquisse, F.J. Corpas, K.J. Gupta, C. Lindermayr, G.J. Loake, J.M. Palma, M. Petřivalský, D. Wendehenne, J.T. HancockAbstractIn this year there is the 40th anniversary of the first publication of plant nitric oxide (NO) emission by Lowell Klepper. In the decades since then numerous milestone discoveries have revealed that NO is a multifunctional molecule in plant cells regulating both plant development and stress responses. Apropos of the anniversary, these authors aim to review and discuss the developm...
Source: Nitric Oxide - September 19, 2019 Category: Chemistry Source Type: research

Nitric oxide and its derivatives in the cancer battlefield
We present here the dual face of nitric oxide and its derivatives in cancer biology. Main derivatives of nitric oxide, such as nitrogen dioxide and peroxynitrite cause cell death by inducing protein and lipid peroxidation and/or DNA damage. Moreover, they control the activity of important protein players within the pro- and anti-apoptotic signaling pathways. Thus, the control of intracellular reactive nitrogen species may become a sophisticated tool in anticancer strategies. (Source: Nitric Oxide)
Source: Nitric Oxide - September 19, 2019 Category: Chemistry Source Type: research

Nitric oxide-loaded chitosan nanoparticles as an innovative antileishmanial platform
Publication date: Available online 18 September 2019Source: Nitric OxideAuthor(s): Fernanda V. Cabral, Milena T. Pelegrino, Ismael P. Sauter, Amedea B. Seabra, Mauro Cortez, Martha S. RibeiroAbstractLeishmaniasis is a neglected tropical disease that demands for new therapeutic strategies due to adverse side effects and resistance development promoted by current drugs. Nitric oxide (NO)-donors show potential to kill Leishmania spp. but their use is limited because of their instability. In this work, we synthesize, characterize, and encapsulate S-nitroso-mercaptosuccinic acid into chitosan nanoparticles (NONPs) and investiga...
Source: Nitric Oxide - September 19, 2019 Category: Chemistry Source Type: research

Nitric oxide stimulates a PKC-Src-Akt signaling axis which increases human immunodeficiency virus type 1 replication in human T lymphocytes
In conclusion, oxidative/nitrosative stress conditions derived from exposure of HIV-1-infected CD4+T lymphocytes to an exogenous NO source trigger a signaling cascade involving PKC, Src kinase and Akt. Activation of this signaling cascade appears to be critical to the establishment of HIV-1 infection.Graphical abstract (Source: Nitric Oxide)
Source: Nitric Oxide - September 18, 2019 Category: Chemistry Source Type: research