• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

Effect of RAB31 silencing on osteosarcoma cell proliferation and migration through the Hedgehog signaling pathway
In this study, we aimed to explore the functions of Ras-related protein Rab-31 (RAB31) in OS-cell proliferation, migration, and invasion as well as its roles in the Hedgehog signaling pathway for better understanding of the mechanism. To assess the detailed regulatory mechanism of RAB31 silencing on OS, both RT-qPCR and Western blot analysis were employed to evaluate the expressions of RAB31 as well as the Hedgehog signaling pathway-related genes. Besides, we also investigated the effects of silenced RAB31 both in vitro and in vivo. First, we found that in OS tissues, both mRNA and protein expressions of RAB31 and PCNA had...
Source: Journal of Bone and Mineral Metabolism - November 23, 2018 Category: Orthopaedics Source Type: research

CYR61/CCN1 Regulates Sclerostin Levels and Bone Maintenance
This article is protected by copyright. All rights reserved
Source: Journal of Bone and Mineral Research - January 1, 2018 Category: Orthopaedics Authors: Gexin Zhao, Bau ‐Lin Huang, Diana Rigueur, Weiguang Wang, Chimay Bhoot, Kemberly R Charles, Jongseung Baek, Subburaman Mohan, Jie Jiang, Karen M. Lyons Tags: Original Article Source Type: research

Effects of SOST Gene Silencing on Proliferation, Apoptosis, Invasion, and Migration of Human Osteosarcoma Cells Through the Wnt/ β-Catenin Signaling Pathway
AbstractOur study explored the effects ofSOST gene silencing on the proliferation, apoptosis, invasion, and migration of human osteosarcoma cells through Wnt/ β-catenin signaling pathway. Fresh tissues were obtained from 108 patients with osteosarcoma and 46 patients with osteochondroma. Human osteosarcoma cells (MG-63, U2-OS, HOS, and Saos-2) and normal osteoblast (hFoB1.19) were selected and cultured. Osteosarcoma cells were grouped randomly into the b lank group, the scrambled control group, and theSOST-siRNA group. Cell proliferation was determined by MTT assay. Cell cycle and apoptosis were tested by flow cytometry. ...
Source: Calcified Tissue International - February 27, 2017 Category: Orthopaedics Source Type: research

Clinical and biological significance of PIM1 kinase in osteosarcoma
This article is protected by copyright. All rights reserved
Source: Journal of Orthopaedic Research - December 19, 2015 Category: Orthopaedics Authors: Yunfei Liao, Yong Feng, Jacson Shen, Yan Gao, Gregory Cote, Edwin Choy, David Harmon, Henry Mankin, Francis Hornicek, Zhenfeng Duan Tags: Research Article Source Type: research

Long noncoding RNA MALAT1 as a potential therapeutic target in osteosarcoma
This article is protected by copyright. All rights reserved
Source: Journal of Orthopaedic Research - November 17, 2015 Category: Orthopaedics Authors: Xianyi Cai, Yunlu Liu, Wen Yang, Yun Xia, Cao Yang, Shuhua Yang, Xianzhe Liu Tags: Research Article Source Type: research

Proteomic Identification of 14‐3‐3ϵ as a Linker Protein Between pERK1/2 Inhibition and BIM Upregulation in Human Osteosarcoma Cells
We examined the expression of Bim after silencing 14‐3‐3ϵ using siRNA. The 14‐3‐3ϵ gene silencing resulted in downregulation of Bim expression after PD98059 treatment. These data indicate that 14‐3‐3ϵ is required for Bim expression and that it has an anti‐cancer effect under pERK1/2 inhibition in 143B cells. By playing an essential role upstream of Bim, 14‐3‐3ϵ may potentially be a coadjuvant factor synergizing the effect of pERK1/2 inhibitors in addition to conventional cytotoxic agents for more effective osteosarcoma treatments. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res
Source: Journal of Orthopaedic Research - February 17, 2014 Category: Orthopaedics Authors: Kyung Ok Kim, Anny C. Hsu, Heon Goo Lee, Neel Patel, Chandhanarat Chandhanayingyong, Thomas Hickernell, Francis Young‐In Lee Tags: Research Article Source Type: research

Role of integrin‐linked kinase in osteosarcoma progression
ABSTRACT Although integrin‐linked kinase (ILK) has been suggested to play a role in the tumorigenesis of a number of human epithelial carcinomas, little is known of its role in musculoskeletal sarcoma. The authors studied ILK expression by immunohistochemistry using osteosarcoma prechemotherapy specimens from 56 patients, and investigated the prognostic implications of the findings obtained. It was found that ILK overexpression was significantly correlated with the presence of distant metastasis (p = 0.008) and that it was an independent prognostic factor for both poor overall survival and poor event‐free survival ...
Source: Journal of Orthopaedic Research - June 19, 2013 Category: Orthopaedics Authors: Seung Hwan Rhee, Ilkyu Han, Mi Ra Lee, Hwan Seong Cho, Joo Han Oh, Han‐Soo Kim Tags: Research Article Source Type: research