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1166 Kinome siRNA screening for the treatment of the XPC cancer-prone disease
Xeroderma Pigmentosium C is a rare autosomal recessive genodermatosis. Patients with this disorder carry a mutation in DNA damage recognition protein XPC belonging to the nucleotide excision repair (NER) pathway. This generates a phenotype characterized by extreme photosensitivity and accumulation of UV-induced DNA damage without a repair potential. XP-C and normal patient-derived cells were used to screen a library of siRNAs aimed at decreasing the expression of all human kinases, given their involvement in different DNA repair pathways.
Source: Journal of Investigative Dermatology - April 17, 2023 Category: Dermatology Authors: F. Kobaisi, E. Sulpice, A. Nasrallah, X. Gidrol, W. Rachidi Source Type: research

622 Regulation of XPC binding dynamics and global nucleotide excision repair by p63 and vitamin D receptor
p63 and the vitamin D receptor (VDR) play important roles in epidermal development and differentiation, but their roles and relationship in the response to ultraviolet (UV) radiation are unclear. Using TERT-immortalized human keratinocytes expressing shRNA targeting p63 in concert with exogenously applied siRNA targeting VDR, we assessed p63 and VDR ’s separate and combined effect on nucleotide excision repair (NER) of UV-induced 6-4 photoproducts (6-4PP) with a focus on the XPC DNA damage recognition protein.
Source: Journal of Investigative Dermatology - July 20, 2022 Category: Dermatology Authors: C. Wong, D.H. Oh Source Type: research

Vitamin D receptor promotes global nucleotide excision repair by facilitating XPC dissociation from damaged DNA
Vitamin D receptor (VDR) is important for normal DNA repair though the mechanism by which it acts is unclear. Following focal ultraviolet irradiation to create subnuclear spots of DNA damage, epidermal keratinocytes from VDR-null mice as well as human epidermal keratinocytes depleted of VDR with siRNA removed pyrimidine(6-4)pyrimidone photoproducts more slowly than control cells. Co-staining with antibodies to XPC, the DNA damage recognition sensor which initiates nucleotide excision repair, revealed that XPC rapidly accumulated at spots of damage and gradually faded in control human keratinocytes.
Source: Journal of Investigative Dermatology - January 28, 2021 Category: Dermatology Authors: Christian T. Wong, Dennis H. Oh Tags: Original Article Source Type: research

643 XPC dissociation from damaged DNA and efficient global nucleotide excision repair depend on vitamin D receptor
Vitamin D and its receptor, VDR, together and independently, have been associated with DNA repair, though the mechanism by which they act is unclear. Upon ultraviolet irradiation through 3 mm pores in otherwise opaque filters to create focal spots of DNA damage, epidermal keratinocytes from both VDR-null mice and human keratinocytes depleted of VDR with siRNA exhibited slower removal of 6-4 photoproducts than normal control cells over 90 minutes. Co-staining with antibodies to XPC, the initial UV-induced DNA damage recognition sensor, revealed that XPC rapidly accumulated at DNA damage foci and gradually faded over 90 minu...
Source: Journal of Investigative Dermatology - June 17, 2020 Category: Dermatology Authors: C. Wong, D.H. Oh Tags: Photobiology Source Type: research

1173 Inducible DNA repair of UV photoproducts depends on vitamin D receptor
Ultraviolet radiation (UV) initiates vitamin D synthesis by converting 7-dehydrocholesterol to pre-vitamin D3 that is converted to 25-hydroxyvitamin D3 (25D3) and then 1,25-dihydroxyvitamin D3 (1,25D3). However, UV also generates DNA damage that is repaired by nucleotide excision repair (NER). We tested the hypothesis that vitamin D signaling elicits compensatory responses to the DNA damage incurred during vitamin D synthesis. Treatment of human keratinocytes with either UVB or with 1,25D3 or 25D3 induced the DNA damage recognition protein, XPC, and induction was suppressed by either siRNA targeting the vitamin D receptor ...
Source: Journal of Investigative Dermatology - April 27, 2018 Category: Dermatology Authors: A. Scandurra, C. Wong, T. Kaur Oberoi, D. Oh Tags: Photobiology Source Type: research

Sirtuin‐6 deficiency exacerbates diabetes‐induced impairment of wound healing
Abstract Delayed wound healing is one of the major complications in diabetes and is characterized by chronic proinflammatory response, and abnormalities in angiogenesis and collagen deposition. Sirtuin family proteins regulate numerous pathophysiological processes, including those involved in promotion of longevity, DNA repair, glycolysis and inflammation. However, the role of sirtuin 6 (SIRT6), a NAD+‐dependent nuclear deacetylase, in wound healing specifically under diabetic condition remains unclear. To analyse the role of SIRT6 in cutaneous wound healing, paired 6‐mm stented wound was created in diabetic db/db mice...
Source: Experimental Dermatology - August 18, 2015 Category: Dermatology Authors: Rajarajan A. Thandavarayan, Venkata Naga Srikanth Garikipati, Darukeshwara Joladarashi, Sahana Suresh Babu, Prince Jeyabal, Suresh K. Verma, Alexander R. Mackie, Mohsin Khan, Somasundaram Arumugam, Kenichi Watanabe, Raj Kishore, Prasanna Krishnamurthy Tags: Original Article Source Type: research

Sirtuin‐6 deficiency exacerbates diabetes induced impairment of wound healing
This article is protected by copyright. All rights reserved.
Source: Experimental Dermatology - May 23, 2015 Category: Dermatology Authors: Rajarajan A Thandavarayan, Venkata Naga Srikanth Garikipati, Joladarashi Darukeshwara, Sahana Suresh Babu, Prince Jeyabal, Suresh K Verma, Alexander R Mackie, Mohsin Khan, Somasundaram Arumugam, Kenichi Watanabe, Raj Kishore, Prasanna Krishnamurthy Tags: Regular Article Source Type: research

A purified Feverfew extract protects from oxidative damage by inducing DNA repair in skin cells via a PI3-kinase-dependent Nrf2/ARE pathway
Conclusions: Therefore, by increasing endogenous defense mechanisms and aid in DNA repair of damaged skin cells via activation of a PI3K-dependent Nrf2/ARE pathway, PD-Feverfew may help protect the skin from numerous environmental aggressors.
Source: Journal of Dermatological Science - September 16, 2013 Category: Dermatology Authors: Karien J. Rodriguez, Heng-Kuan Wong, Thierry Oddos, Michael Southall, Balz Frei, Simarna Kaur Tags: Regular Articles Source Type: research

Thy-1 knockdown retards wound repair in mouse skin
Conclusion: These data suggest that blocking Thy-1 at wound areas using siRNA reduces repair and affects the re-epithelialization and over-expression of TGF-β1 of the wound during the skin healing process.
Source: Journal of Dermatological Science - January 11, 2013 Category: Dermatology Authors: Min-Jung Lee, Jeong-Oh Shin, Han-Sung Jung Tags: Regular articles Source Type: research