Vitamin D receptor promotes global nucleotide excision repair by facilitating XPC dissociation from damaged DNA

Vitamin D receptor (VDR) is important for normal DNA repair though the mechanism by which it acts is unclear. Following focal ultraviolet irradiation to create subnuclear spots of DNA damage, epidermal keratinocytes from VDR-null mice as well as human epidermal keratinocytes depleted of VDR with siRNA removed pyrimidine(6-4)pyrimidone photoproducts more slowly than control cells. Co-staining with antibodies to XPC, the DNA damage recognition sensor which initiates nucleotide excision repair, revealed that XPC rapidly accumulated at spots of damage and gradually faded in control human keratinocytes.
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Tags: Original Article Source Type: research