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Specialty: Dermatology
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Total 16 results found since Jan 2013.

1166 Kinome siRNA screening for the treatment of the XPC cancer-prone disease
Xeroderma Pigmentosium C is a rare autosomal recessive genodermatosis. Patients with this disorder carry a mutation in DNA damage recognition protein XPC belonging to the nucleotide excision repair (NER) pathway. This generates a phenotype characterized by extreme photosensitivity and accumulation of UV-induced DNA damage without a repair potential. XP-C and normal patient-derived cells were used to screen a library of siRNAs aimed at decreasing the expression of all human kinases, given their involvement in different DNA repair pathways.
Source: Journal of Investigative Dermatology - April 17, 2023 Category: Dermatology Authors: F. Kobaisi, E. Sulpice, A. Nasrallah, X. Gidrol, W. Rachidi Source Type: research

The paranuclear dot in Merkel cell carcinoma, how does it form and what does it do?
Background and aims: Staining for Cytokeratin 20 (CK20) and other intermediate filaments in a paranuclear dot pattern is a histological feature used to diagnose the neuroendocrine skin cancer Merkel cell carcinoma (MCC). The functional significance of these paranuclear protein aggregates is unknown. Therefore, we sought to investigate the structure and function of the paranuclear dot in MCC. Methods: In native tumors and MCC cell lines we used: immunostaning, microscopy, chemical inhibition, siRNA knockdown, and cell death modulators to study the paranuclear dot.
Source: Journal of Investigative Dermatology - September 21, 2022 Category: Dermatology Authors: Natasha T. Hill, Loren Collado, Tyler Kellenberger, Kunio Nagashima, Serena Vilasi, Paul W. Harms, Isaac Brownell Source Type: research

Discovering the role of FZD4 Gene in human cutaneous squamous cell carcinoma
Conclusion: The results indicated that FZD4 may play as a tumor suppressor gene in the pathogenesis of CSCC.
Source: Indian Journal of Dermatology - December 9, 2021 Category: Dermatology Authors: Ke Zhang Qun Lv Liming Li Mingjun Jiang Fang Fang Source Type: research

Allele-specific sirna corrects aberrant cellular phenotype in keratitis-ichthyosis-deafness syndrome keratinocytes
Keratitis-ichthyosis-deafness (KID) syndrome is a severe, untreatable condition characterized by ocular, auditory and cutaneous abnormalities, with major complications of infection and skin cancer. 86% of cases are caused by a heterozygous missense mutation (c.148G>A, p.D50N) in the GJB2 gene, encoding gap junction protein connexin 26 (Cx26), which alters gating properties of Cx26 channels in a dominant manner. We hypothesized that a mutant-allele-specific siRNA (AS-siRNA) could rescue the cellular phenotype in patient keratinocytes.
Source: Journal of Investigative Dermatology - November 5, 2019 Category: Dermatology Authors: Ming Yang Lee, Hong-Zhan Wang, Thomas W. White, Tony Brooks, Alan Pittman, Heerni Halai, Anastasia Petrova, Diane Xu, Stephen L. Hart, Veronica A. Kinsler, Wei-Li Di Tags: Original Article Source Type: research

812 Type VII collagen and Nesprin 2, LINCing the basement membrane to altered cell cycle and increased DNA damage
Epidermolysis Bullosa is a severe blistering disease caused by mutations in basement membrane genes and is characterised by severe blistering of the skin and increased predisposition to cancer. To investigate the role of the skin basement membrane, siRNA knockdown of type IV, VII and XVII collagens was performed in primary keratinocytes. Global transcriptomic analysis was carried out using RNA-Seq. Dysregulation of genes involved in DNA damage and cell cycle control was seen in cells with Col7 and Col17 knockdown.
Source: Journal of Investigative Dermatology - April 27, 2018 Category: Dermatology Authors: S. Marsh, M.P. Caley, V. Martins, M. Chen, J. McGrath, M. Barnes, E.A. O'Toole Tags: Genetic Disease, Gene Regulation, and Gene Therapy Source Type: research

192 Loss of keratinocyte type VII collagen induces increased DNA damage in vitro and in vivo
Epidermolysis Bullosa is a severe blistering disease caused by mutations in basement membrane genes and is characterised by severe blistering of the skin and increased predisposition to cancer. To investigate the function of the skin basement membrane, siRNA knockdown of type IV, VII (Col7) and XVII (Col17) collagens was performed in primary neonatal foreskin keratinocytes. Global transcriptomic analysis was carried out using RNA-Seq. Pathway analysis revealed dysregulation of genes involved in DNA damage and cell cycle control in cells with Col7 and Col17 knockdown predicting reduced proliferation of siCol7 cells and incr...
Source: Journal of Investigative Dermatology - September 8, 2017 Category: Dermatology Authors: S. Marsh, M. Caley, V. Martins, M.R. Barnes, M. Chen, E.A. O ’Toole Tags: Genetics and Cell Based Therapy Source Type: research

103 Blockade of hippo pathway effector YAP1 with shRNA attenuates vascular development in Hemangioma Bend3 cells in vivo
Angiopoietin-2 (Ang2) has been demonstrated to the primary driver of Infantile hemangioma (IH), the most common tumor of infancy and occurs in 4% –12% of infants. YAP1, the Yes-associated Protein, is implicated in the regulation of Ang2. We assessed the presence of YAP1 in human hemangiomas and the functional significance of YAP1 in bend3 cells, a validated model of human hemangioma that is dependent on Ang2. YAP1 is highly expressed in all human hemangiomas in the endothelial compartment. Using siRNA lentivirus to YAP1, we downregulated YAP1 in Bend3.
Source: Journal of Investigative Dermatology - April 12, 2017 Category: Dermatology Authors: M. Bonner, R. Pankove, S. Rao, A. Costa, B. Shetata, J. Elsey, J.L. Arbiser Tags: Carcinogenesis and Cancer Genetics Source Type: research

Thick melanoma in Tuscany.
CONCLUSIONS: If we want to obtain better results on diagnosis of skin melanoma we have to think a new strategy. At least to think over the educational messages discriminating people more at risk of incidence of melanoma from people more at risk to die from melanoma, and to renewed active involvement of the Gen- eral Practitioners . PMID: 28290624 [PubMed - as supplied by publisher]
Source: Giornale Italiano di Dermatologia e Venereologia - March 17, 2017 Category: Dermatology Tags: G Ital Dermatol Venereol Source Type: research

Inhibition of HSP90 exerts anti-tumor effect on angiosarcoma: Involvement of VEGF signaling pathway.
CONCLUSIONS: HSP90 could be a novel therapeutic target for angiosarcoma. This article is protected by copyright. All rights reserved. PMID: 28078663 [PubMed - as supplied by publisher]
Source: The British Journal of Dermatology - January 11, 2017 Category: Dermatology Authors: Yamada-Kanazawa S, Kajihara I, Fukushima S, Jinnin M, Masuzawa M, Masuzawa M, Amoh Y, Hoshina D, Abe R, Ihn H Tags: Br J Dermatol Source Type: research

043 Mechanism of action of propranolol in Infantile Hemangioma: New insights from a xenograft model
8 years after propranolol was found efficacious in infantile hemangioma (IH), therapeutic mechanisms remain elusive. It has been shown, in an ovarian cancer model, that ADRB2 signaling is key for chronic stress induced tumor growth. In this model, tumor promotion is abolished by propranolol or ADRB2 siRNA but not by ADRB1 siRNA. In IH patients, after oral administration of 3 mg/kg/day of propranolol, plasma Cmax is below 1 μM, whereas propranolol has been used in vitro at 100 μM and up to 50 mg/kg in mouse models.
Source: Journal of Investigative Dermatology - August 16, 2016 Category: Dermatology Authors: F. Moisan, J. Nissen, P. Kaulanjan-Checkmodine, S. Prey, P. Dufourcq, T. Couffinhal, H. Rezvani, A. Taieb Tags: Clinical Outcomes Source Type: research

Cell division cycle‐associated protein 1 as a new melanoma‐associated antigen
Abstract Immune checkpoint inhibitors have increased the median survival of melanoma patients. To improve their effects, antigen‐specific therapies utilizing melanoma‐associated antigens should be developed. Cell division cycle‐associated protein 1 (CDCA1), which has a specific function at the kinetochores for stabilizing microtubule attachment, is overexpressed in various cancers. CDCA1, which is a member of cancer–testis antigens, does not show detectable expression levels in normal tissues. Quantitative reverse transcription polymerase chain reaction and immunoblotting analyses revealed that CDCA1 was expressed ...
Source: The Journal of Dermatology - May 29, 2016 Category: Dermatology Authors: Aki Tokuzumi, Satoshi Fukushima, Azusa Miyashita, Satoshi Nakahara, Yosuke Kubo, Junji Yamashita, Miho Harada, Kayo Nakamura, Ikko Kajihara, Masatoshi Jinnin, Hironobu Ihn Tags: Original Article Source Type: research

Cell division cycle ‐associated protein 1 as a new melanoma‐associated antigen
Abstract Immune checkpoint inhibitors have increased the median survival of melanoma patients. To improve their effects, antigen‐specific therapies utilizing melanoma‐associated antigens should be developed. Cell division cycle‐associated protein 1 (CDCA1), which has a specific function at the kinetochores for stabilizing microtubule attachment, is overexpressed in various cancers. CDCA1, which is a member of cancer–testis antigens, does not show detectable expression levels in normal tissues. Quantitative reverse transcription polymerase chain reaction and immunoblotting analyses revealed that CDCA1 was expressed ...
Source: The Journal of Dermatology - May 29, 2016 Category: Dermatology Authors: Aki Tokuzumi, Satoshi Fukushima, Azusa Miyashita, Satoshi Nakahara, Yosuke Kubo, Junji Yamashita, Miho Harada, Kayo Nakamura, Ikko Kajihara, Masatoshi Jinnin, Hironobu Ihn Tags: Original Article Source Type: research

Aldo‐keto reductase 1C3 is overexpressed in skin squamous cell carcinoma (SCC) and affects SCC growth via prostaglandin metabolism
Abstract Aldo‐keto reductase 1C3 (AKR1C3) is an enzyme involved in metabolizing prostaglandins (PGs) and sex hormones. It metabolizes PGD2 to 9α11β‐PGF2, diverting the spontaneous conversion of PGD2 to the PPARγ agonist, 15‐Deoxy‐Delta‐12, 14‐prostaglandin J2 (15d‐PGJ2). AKR1C3 is overexpressed in various malignancies, suggesting a tumor promoting function. This work investigates AKR1C3 expression in human non‐melanoma skin cancers, revealing overexpression in squamous cell carcinoma (SCC). Effects of AKR1C3 overexpression were then evaluated using three SCC cell lines. AKR1C3 was detected in all SCC cel...
Source: Experimental Dermatology - July 16, 2014 Category: Dermatology Authors: Alon Mantel, Amanda Carpenter‐Mendini, JoAnne VanBuskirk, Alice P. Pentland Tags: Original Article Source Type: research

Aldo‐keto Reductase 1C3 (AKR1C3) is overexpressed in skin squamous cell carcinoma (SCC) and affects SCC growth via prostaglandin metabolism
This article is protected by copyright. All rights reserved.
Source: Experimental Dermatology - June 10, 2014 Category: Dermatology Authors: Alon Mantel, Amanda Carpenter‐Mendini, JoAnne VanBuskirk, Alice P. Pentland Tags: Regular Article Source Type: research