• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

Delivering siRNA Compounds During HOPE to Modulate Organ Function: A Proof-of-concept Study in a Rat Liver Transplant Model
Conclusions. FAS inhibition through siRNA therapy decreases the severity of IRI after LT in a SCS protocol; however the association of siRNA therapy with a HOPE perfusion model is very challenging. Future studies using better designed siRNA compounds and appropriate doses are required to prove the siRNA therapy effectiveness during liver HOPE liver perfusion.
Source: Transplantation - August 1, 2022 Category: Transplant Surgery Tags: Original Basic Science Source Type: research

Review of Current Machine Perfusion Therapeutics for Organ Preservation
Because of the high demand of organs, the usage of marginal grafts has increased. These marginal organs have a higher risk of developing ischemia-reperfusion injury, which can lead to posttransplant complications. Ex situ machine perfusion (MP), compared with the traditional static cold storage, may better protect these organs from ischemia-reperfusion injury. In addition, MP can also act as a platform for dynamic administration of pharmacological agents or gene therapy to further improve transplant outcomes. Numerous therapeutic agents have been studied under both hypothermic (1–8°C) and normothermic settings. Here, we...
Source: Transplantation - August 26, 2020 Category: Transplant Surgery Tags: Reviews Source Type: research

The role of machine perfusion in liver xenotransplantation.
Abstract PURPOSE OF REVIEW: To review the role of machine perfusion in advancing the study and clinical application of liver xenotransplantation to liver transplantation. RECENT FINDINGS: Recent multicenter trial has shown the benefits of normothermic machine perfusion (NMP) in the assessment and selection of suitable allografts for liver transplantation, especially marginal liver allografts. Advances in ex-vivo therapeutic intervention with proof-of-concept studies demonstrating successful ex-vivo genetic modification of donor allografts and blockade of gene expression with siRNA. SUMMARY: Xenotransplan...
Source: Current Opinion in Organ Transplantation - August 20, 2020 Category: Transplant Surgery Authors: MacConmara M, Feizpour CA, Shubin A, Vagefi PA Tags: Curr Opin Organ Transplant Source Type: research

First Report of siRNA Uptake (for RNA Interference) During Ex Vivo Hypothermic and Normothermic Liver Machine Perfusion
No abstract available
Source: Transplantation - February 27, 2019 Category: Transplant Surgery Tags: In Brief Source Type: research

Sirtuin 1 Attenuates Inflammation and Hepatocellular Damage in Liver Transplant Ischemia ‐Reperfusion: From Mouse‐to‐Human
In conclusion, our translational study is the first to identify SIRT1 as a regulator of hepatocellular function in human liver transplant recipients under IR‐stress. By targeting innate and adaptive immune activation, manipulation of SIRT1 signaling should be considered as novel means to combat inflammation in liver transplantation. This article is protected by copyright. All rights reserved.
Source: Liver Transplantation - July 1, 2017 Category: Transplant Surgery Authors: Kojiro Nakamura, Shoichi Kageyama, Bibo Ke, Takehiro Fujii, Rebecca A. Sosa, Elaine F. Reed, Nakul Datta, Ali Zarrinpar, Ronald W. Busuttil, Jerzy W. Kupiec ‐Weglinski Tags: Original Articles Source Type: research

Calcineurin Inhibitors Downregulate HNF-1β and May Affect the Outcome of HNF1B Patients After Renal Transplantation
Conclusions: Because HNF1B-related disease is a heterozygous condition, CNIs used to prevent rejection may induce reduced expression of the nonmutated allele of HNF1B leading to a superimposed defect of HNF-1β transcriptional activity. Taking into account the specific risk of posttransplantation diabetes mellitus and liver disorders in HNF1B patients, these findings advocate for in-depth characterization of pathways that regulate HNF1B and plead for considering individually tailored graft management that may include a CNI-free immunosuppressive regimen. Interventional studies will have to confirm this individualized approach.
Source: Transplantation - August 24, 2016 Category: Transplant Surgery Tags: Original Clinical Science-General Source Type: research

ATF6 Mediates a Pro‐Inflammatory Synergy Between ER Stress and TLR Activation in the Pathogenesis of Liver Ischemia‐Reperfusion Injury
Although the roles of the metabolic stress in organ ischemia‐reperfusion injury (IRI) have been well recognized, the question of whether and how these stress responses regulate innate immune activation against IR remains unclear. In a murine liver partial warm ischemia mode, we showed that prolonged ischemia triggered endoplasmic reticulum (ER) stress response, particularly, the activating transcription factor 6 (ATF6) branch, in liver Kupffer cells (KCs) and altered their responsiveness against Toll‐like receptor (TLR) stimulation. Ischemia‐primed cells increased pro‐, but decreased anti‐, inflammatory cytokine ...
Source: American Journal of Transplantation - June 1, 2014 Category: Transplant Surgery Authors: J. Rao, S. Yue, Y. Fu, J. Zhu, X. Wang, R. W. Busuttil, J. W. Kupiec‐Weglinski, L. Lu, Y. Zhai Tags: Original Article Source Type: research

Regulation of Heme Oxygenase 1 Expression by miR-27b With Stem Cell Therapy for Liver Regeneration in Rats
In this study, we studied the role of miR-27b in ASCs and their regenerative potential after partial liver resection in rats. ASCs transfected with control siRNA or miR-27b were intravenously injected into autologous rats undergoing 70% partial hepatectomy (PH). Our data showed that the regenerative capacities of ASCs with overexpressed miR-27b were significantly higher compared with control ASCs. However, the enhanced regeneration, hepatic differentiation, and suppressed liver inflammation, as well as fibrotic activity, were significantly reverted by ZnPP coadministration (heme oxygenase-1 [HO-1] inhibitor) indicating an ...
Source: Transplantation Proceedings - May 1, 2014 Category: Transplant Surgery Authors: K.-D. Chen, L.-W. Hsu, S. Goto, K.-T. Huang, T. Nakano, W.-T. Weng, C.-Y. Lai, Y.-R. Kuo, K.-W. Chiu, C.-C. Wang, Y.-F. Cheng, C.-C. Lin, Y.-Y. Ma, C.-L. Chen Tags: Experimental Transplantation Source Type: research