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Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing
In conclusion, the method of NK cell transfection described in our present study is highly efficient, does not require expensive dedicated structures necessary for viral transduction and avoids possible risks associated with the use of viral vectors. Importantly, it may be applied to NK cells or NK-92 cell line, greatly improving their anti-tumor activity and providing a new NK cell-based platform for new protocols of adoptive immuno-therapy of cancer. Ethics Statement The Ethical Committee of IRCCS Bambino Gesù Pediatric Hospital approved the study (825/2014). Author Contributions TI designed and performed res...
Source: Frontiers in Immunology - April 29, 2019 Category: Allergy & Immunology Source Type: research

Phosphatase of regenerating liver maintains cellular magnesium homeostasis
Phosphatase of regenerating liver (PRL) is highly expressed in malignant cancers and promotes cancer progression. Recent studies have suggested its functional relationship with Mg2+, but the importance and molecular details of this relationship remain unknown. Here, we report that PRL expression is regulated by Mg2+ and PRL protects cells from apoptosis under Mg2+-depleted conditions. When cultured cells were subjected to Mg2+ depletion, endogenous PRL protein levels increased significantly. siRNA-mediated knockdown of endogenous PRL did not significantly affect cell proliferation under normal culture conditions, but it in...
Source: Biochemical Journal - March 26, 2018 Category: Biochemistry Authors: Yoshida, A., Funato, Y., Miki, H. Tags: Research Articles Source Type: research

Phosphatase of regenerating liver maintains cellular magnesium homeostasis.
Abstract Phosphatase of regenerating liver (PRL) is highly expressed in malignant cancers and promotes cancer progression. Recent studies have suggested its functional relationship with Mg2+, but the importance and molecular details of this relationship remain unknown. Here, we report that PRL expression is regulated by Mg2+ and PRL protects cells from apoptosis under Mg2+-depleted conditions. When cultured cells were subjected to Mg2+ depletion, endogenous PRL protein levels increased significantly. siRNA-mediated knockdown of endogenous PRL did not significantly affect cell proliferation under normal culture con...
Source: The Biochemical Journal - February 27, 2018 Category: Biochemistry Authors: Yoshida A, Funato Y, Miki H Tags: Biochem J Source Type: research

Regulation of Chromatin Assembly and Cell Transformation by Formaldehyde Exposure in Human Cells
Conclusions: We propose that the inhibition of chromatin assembly represents a novel mechanism of cell transformation induced by the environmental and occupational chemical carcinogen FA. https://doi.org/10.1289/EHP1275 Received: 25 October 2016 Revised: 19 May 2017 Accepted: 23 May 2017 Published: 21 September 2017 Address correspondence to C. Jin, Dept. of Environmental Medicine, New York University School of Medicine, 57 Old Forge Rd., Tuxedo Park, NY 10987 USA. Telephone: (845) 731-3602. Email: Chunyuan.jin@nyumc.org *Current affiliation: Medical School of Nanjing University, Nanjing, China. †Current affiliatio...
Source: EHP Research - September 21, 2017 Category: Environmental Health Authors: Daniil Lyalko Tags: Research Source Type: research