• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

The Exocyst Complex is Required for the Trafficking and Delivery of KCa3.1 to the Basolateral Membrane of Polarized Epithelia
Am J Physiol Cell Physiol. 2023 May 1. doi: 10.1152/ajpcell.00374.2022. Online ahead of print.ABSTRACTControl of the movement of ions and water across epithelia is essential for homeostasis. Changing the number or activity of ion channels at the plasma membrane is a significant regulator of epithelial transport. In polarized epithelia, the intermediate-conductance calcium-activated potassium channel, KCa3.1 is delivered to the basolateral membrane where it generates and maintains the electrochemical gradients required for epithelial transport. The mechanisms that control the delivery of KCa3.1 to the basolateral membrane a...
Source: Am J Physiol Cell Ph... - May 1, 2023 Category: Cytology Authors: Matthew J E Logue Rachel E Farquhar Yoakim Eckhoff-Bj örngard Tanya T Cheung Daniel C Devor Fiona J McDonald Kirk L Hamilton Source Type: research

p38 MAPK activation and STIM1-Orai3 association mediate TRPC6 externalization
Am J Physiol Cell Physiol. 2023 Apr 24. doi: 10.1152/ajpcell.00425.2022. Online ahead of print.ABSTRACTEndothelial cell (EC) migration is critical for the repair of monolayer disruption following angioplasties, but migration is inhibited by lipid oxidation products, including lysophosphatidylcholine (lysoPC), which open canonical transient receptor potential 6 (TRPC6) channels. TRPC6 activation requires an increase in intracellular Ca2+ concentration ([Ca2+]i), the source of which is unknown. LysoPC can activate phospholipase A2 to release arachidonic acid (ArA). ArA can activate arachidonic acid-regulated calcium (ARC) ch...
Source: Am J Physiol Cell Ph... - April 24, 2023 Category: Cytology Authors: Pinaki Chaudhuri Priya Putta Michael A Rosenbaum Linda M Graham Source Type: research

Activation of the PKA Signaling Pathway Stimulates Oxalate Transport by Human Intestinal Caco2-BBE Cells.
Abstract Most kidney stones are composed of calcium oxalate, and small increases in urine oxalate enhance the stone risk. The mammalian intestine plays a crucial role in oxalate homeostasis and we had recently reported that Oxalobacter-derived factors stimulate oxalate transport by human intestinal Caco2-BBE (C2) cells through PKA activation. We therefore evaluated whether intestinal oxalate transport is directly regulated by PKA activation. To this end, PKA is activated with forskolin and IBMX (F/I). F/I significantly stimulated (3.7-fold) 14C-oxalate transport by C2 cells (≥ 49% of which is mediated by the oxa...
Source: Am J Physiol Cell Ph... - December 10, 2019 Category: Cytology Authors: Arvans D, Alshaikh A, Bashir M, Weber C, Hassan H Tags: Am J Physiol Cell Physiol Source Type: research

Adenosinergic Signaling Inhibits Oxalate Transport by Human Intestinal Caco2-BBE Cells Through the A2B Adenosine Receptor.
Abstract Most kidney stones (KS) are composed of calcium oxalate, and small increases in urine oxalate affect the stone risk. Intestinal oxalate secretion mediated by anion exchanger SLC26A6 (PAT1) plays a crucial role in limiting net absorption of ingested oxalate; thereby preventing hyperoxaluria and related KS, reflecting the importance of understanding regulation of intestinal oxalate transport. We previously showed that ATP and UTP inhibit oxalate transport by human intestinal Caco2-BBE cells (C2). Since ATP is rapidly degraded to adenosine (ADO), we examined whether intestinal oxalate transport is regulated ...
Source: Am J Physiol Cell Ph... - July 18, 2018 Category: Cytology Authors: Jung D, Alshaikh A, Ratakonda S, Bashir M, Amin MR, Jeon S, Stevens J, Sharma S, Ahmed W, Musch M, Hassan H Tags: Am J Physiol Cell Physiol Source Type: research

Integration of TRPC6 and NADPH Oxidase Activation in Lysophosphatidylcholine-induced TRPC5 Externalization.
Abstract Lipid oxidation products, including lysophosphatidylcholine (lysoPC), activate TRPC6, and the subsequent increase in intracellular Ca(2+) leads to TRPC5 activation. The goal of this study is to elucidate the steps in the pathway between TRPC6 activation and TRPC5 externalization. Following TRPC6 activation by lysoPC, extracellular regulated kinase (ERK) is phosphorylated. This leads to phosphorylation of p47(phox) and subsequent NADPH oxidase activation with increased production of reactive oxygen species. ERK activation requires TRPC6 opening and influx of Ca(2+) as evidenced by the failure of lysoPC to ...
Source: Am J Physiol Cell Ph... - August 23, 2017 Category: Cytology Authors: Chaudhuri P, Rosenbaum MA, Birnbaumer L, Graham LM Tags: Am J Physiol Cell Physiol Source Type: research