Activation of the PKA Signaling Pathway Stimulates Oxalate Transport by Human Intestinal Caco2-BBE Cells.

Activation of the PKA Signaling Pathway Stimulates Oxalate Transport by Human Intestinal Caco2-BBE Cells. Am J Physiol Cell Physiol. 2019 Dec 11;: Authors: Arvans D, Alshaikh A, Bashir M, Weber C, Hassan H Abstract Most kidney stones are composed of calcium oxalate, and small increases in urine oxalate enhance the stone risk. The mammalian intestine plays a crucial role in oxalate homeostasis and we had recently reported that Oxalobacter-derived factors stimulate oxalate transport by human intestinal Caco2-BBE (C2) cells through PKA activation. We therefore evaluated whether intestinal oxalate transport is directly regulated by PKA activation. To this end, PKA is activated with forskolin and IBMX (F/I). F/I significantly stimulated (3.7-fold) 14C-oxalate transport by C2 cells (≥ 49% of which is mediated by the oxalate transporter SLC26A6 [A6]), an effect completely blocked by the PKA inhibitor H89, indicating that it is PKA-dependent. PKA stimulation of intestinal oxalate transport is not cell-line specific since F/I similarly stimulated oxalate transport by the human intestinal T84 cells. F/I significantly increased (2.5-fold) A6 surface protein expression using immunocytochemistry. Assessing 14C-oxalate transport as a function of increasing 14C-oxalate concentration in the flux medium showed that that the observed stimulation is due to F/I-induced increase (1.8-fold) in Vmax and reduction (2-fold) in Km. siRNA knockdown studies showed that significa...
Source: Am J Physiol Cell Ph... - Category: Cytology Authors: Tags: Am J Physiol Cell Physiol Source Type: research

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ConclusionThe results of this randomized, double-blind, placebo-controlled trial suggest that PVB should be considered an effective strategy to reduce opioid requirement and improve pain control for patients undergoing PCNL.
Source: World Journal of Urology - Category: Urology & Nephrology Source Type: research
LIFESTYLE RISK FACTORS AND BONE MASS IN RECURRENT STONE-FORMING PATIENTS: A CROSS-SECTIONAL STUDY IN 144 SUBJECTS. Acta Clin Croat. 2019 Sep;58(3):439-445 Authors: Marić I, Kizivat T, Smolić M, Smolić R, Opačak-Bernardi T, Šolić K, Roguljić H, Milas Ahić J, Tucak A, Mihaljević I Abstract Patients with urolithiasis, particularly hypercalciuria, may have reduced bone mineral density (BMD). There are numerous risk factors contributing to reduction of BMD such as advanced age, sedentary lifestyle, smoking, low calcium intake, etc. The aim of our study was to investigate the association of li...
Source: Acta Clinica Croatica - Category: General Medicine Tags: Acta Clin Croat Source Type: research
Phys. Chem. Chem. Phys., 2020, Accepted Manuscript DOI: 10.1039/C9CP06499K, PaperShanthil Madhavan, Sandeep K, P K Sajith Precipitation and dissolution of calcium oxalate monohydrate (CaOx) crystals are relevant due to their major role in kidney stone diseases. To such an extent, small molecules and ions can act... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Phys. Chem. Chem. Phys. latest articles - Category: Chemistry Authors: Source Type: research
In conclusion, urinary SS varies throughout the day, being higher for CaOx and CaP at night, and in the early morning for UA. For CaOx and UA, the overnight and 12–4 pm urine samples, respectively, contribute most to the variability ob served in the SS of 24-h urine.
Source: Urolithiasis - Category: Urology & Nephrology Source Type: research
Conditions:   Shared Decision-making;   Kidney Stone;   Emergencies;   Radiation Exposure;   Communication Interventions:   Behavioral: Decision Aid;   Other: Standardized Educational Material (informational pamphlet) Sponsors:   Baystate Medical Center;   Agency for Healthcare Research and Quality (AHRQ) Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
PMID: 31958838 [PubMed - in process]
Source: Annals of Internal Medicine - Category: Internal Medicine Authors: Tags: Ann Intern Med Source Type: research
Ureteritis cystica is rare, benign entity that associates with chronic urothelial irritation such as recurrent urinary tract infection or nephrolithiasis. It is often diagnosed incidentally on routine imaging or ureteroscopy in asymptomatic individuals. In this case report, we present the retrograde pyelogram and ureteroscopy images of a rare case of extensive unilateral ureteritis cystica in a 78-year-old female presenting for elective stone surgery.
Source: Urology - Category: Urology & Nephrology Authors: Source Type: research
Conditions:   Shared Decision-making;   Kidney Stone;   Emergencies;   Radiation Exposure;   Communication Interventions:   Behavioral: Decision Aid;   Other: Standardized Educational Material (informational pamphlet) Sponsors:   Baystate Medical Center;   Agency for Healthcare Research and Quality (AHRQ) Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
CONCLUSIONS: Although preoperative use of silodosin facilitated only an insignificant positive effect on UAS placement failure, it eased the F-URS procedure by reducing the ETBT, ETUOT, and AAST in seconds. More studies are needed to make an exact conclusion. PMID: 31950923 [PubMed - in process]
Source: Archivos Espanoles de Urologia - Category: Urology & Nephrology Tags: Arch Esp Urol Source Type: research
Authors: Çam İ, Şengül E, Kale A, Ulukaradag E, Öztürk O, Yalnız A, Çakır Ö PMID: 31941398 [PubMed - as supplied by publisher]
Source: Journal of Obstetrics and Gynaecology - Category: OBGYN Tags: J Obstet Gynaecol Source Type: research
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