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Source: Journal of Cellular Biochemistry

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Total 134 results found since Jan 2013.

Mechanisms of ceramide‐induced COX‐2‐dependent apoptosis in human ovarian cancer OVCAR‐3 cells partially overlapped with resveratrol
Abstract Ceramide is a member of the sphingolipid family of bioactive molecules demonstrated to have profound, diverse biological activities. Ceramide is a potential chemotherapeutic agent via the induction of apoptosis. Exposure to ceramide activates extracellular‐signal‐regulated kinases (ERK)1/2‐ and p38 kinase‐dependent apoptosis in human ovarian cancer OVCAR‐3 cells, concomitant with an increase in the expression of COX‐2 and p53 phosphorylation. Blockade of cyclooxygenase‐2 (COX‐2) activity by siRNA or NS398 correspondingly inhibited ceramide‐induced p53 Ser‐15 phosphorylation and apoptosis; thus ...
Source: Journal of Cellular Biochemistry - June 11, 2013 Category: Biochemistry Authors: Hung‐Yun Lin, Dominique Delmas, Ole Vang, Tze‐Chen Hsieh, Sharon Lin, Guei‐Yun Cheng, Hsiao‐Ling Chiang, Chiao En Chen, Heng‐Yuan Tang, Dana R. Crawford, Jacqueline Whang‐Peng, Jaulang Hwang, Leroy F. Liu, Joseph M. Wu Tags: Article Source Type: research

Knockdown of PU.1 as lncRNA inhibits adipogenesis through enhancing PU.1 1 mRNA translation
Abstract PU.1 is an Ets family transcription factor involved in the myelo‐lymphoid differentiation. We have previously demonstrated that PU.1 is also expressed in the adipocyte lineage. However, the expression levels of PU.1 mRNA and protein in preadipocytes do not match the levels in mature adipocytes. PU.1 mRNA level is higher in preadipocytes, whereas its protein is expressed in the adipocytes but not in the preadipocytes. The underlying mechanism remains elusive. Here, we find that miR‐155 knockdown or overexpression has no effect on the levels of PU.1 mRNA and protein in preadipocytes or adipocytes. MiR‐155 regu...
Source: Journal of Cellular Biochemistry - June 7, 2013 Category: Biochemistry Authors: Wei‐Jun Pang, Li‐Gen Lin, Yan Xiong, Ning Wei, Yu Wang, Qing‐Wu Shen, Gong‐She Yang Tags: Article Source Type: research

L‐cysteine and hydrogen sulfide increase PIP3 and AMPK/PPARγ expression and decrease ROS and vascular inflammation markers in high glucose treated human U937 monocytes
This study examined whether impaired LC or H2S levels affect vascular inflammation markers in diabetes. Human U937 monocytic cells were treated with high‐glucose (HG, 25 mM, 20 h) in the presence or absence of LC (100, 500, or 1000 µM, an endogenous precursor of H2S) or Na2S (5 or 25 µM, an exogenous source of H2S). Both LC and Na2S supplementation decreased intracellular ROS production and increased cellular PIP3 (phosphatidylinositol‐3,4,5‐trisphosphate) in HG‐exposed cells. The effect of LC on PIP3 was prevented by propargylglycine, an inhibitor of cystathionine‐γ‐lyase (CSE) that catalyzes H2S fo...
Source: Journal of Cellular Biochemistry - June 4, 2013 Category: Biochemistry Authors: Prasenjit Manna, Sushil K. Jain Tags: Article Source Type: research

The protein ERp57 contributes to EGF receptor signaling and internalization in MDA‐MB‐468 breast cancer cells
Abstract The disulfide isomerase ERp57 is a soluble protein mainly located in the endoplasmic reticulum, where it acts in the quality control of newly synthesized glycoproteins, in association with calreticulin and calnexin. It has been also detected in other cell compartments, such as the cytosol, the plasma membrane and the nucleus. In these locations it is implicated in various processes, participating in the rapid response to calcitriol, modulating the activity of STAT3 and being requested for the pre‐apoptotic exposure of calreticulin on the plasma membrane. In the present work, the involvement of ERp57 in the activ...
Source: Journal of Cellular Biochemistry - May 20, 2013 Category: Biochemistry Authors: Gaucci Elisa, Altieri Fabio, Carlo Turano, Chichiarelli Silvia Tags: Article Source Type: research

Helicobacter pylori activates NF‐κB by inducing Ubc13‐mediated ubiquitination of lysine 158 of TAK1
Abstract The Helicobacter pylori virulence factor CagA targets a variety of host proteins to alter different cellular responses, including the induction of pro‐inflammatory cytokines. We have previously shown that CagA‐facilitated lysine 63‐linked ubiquitination of TAK1 is essential for the H. pylori‐induced NF‐κB activation and the expression of proinflammatory cytokines. However, the molecular mechanism for TAK1 ubiquitination and activation in H. pylori‐mediated NF‐κB activation remains elusive. Here, we identify lysine 158 of TAK1 as the key residue undergoing lysine 63‐linked ubiquitination in respon...
Source: Journal of Cellular Biochemistry - April 19, 2013 Category: Biochemistry Authors: Acacia Lamb, JinJing Chen, Steven R. Blanke, Lin‐Feng Chen Tags: Article Source Type: research

Role of JAK2–STAT3 in TLR2‐mediated tissue factor expression
This study investigated the mechanism regulating TF expression in macrophages using Pam3CSK4, a TLR2 ligand. Pam3CSK4 induced TF expression in two types of macrophages (Raw264.7 and BMDM), but not in TLR2 KO mice derived BMDM. Pam3CSK4 induced TF expression was inhibited by pretreatment with pan‐JAK inhibitor or JAK2 inhibitor AG490. JAK2 knock‐down by siRNA inhibited Pam3CSK4 induced TF expression. Pam3CSK4 stimulated STAT3 phosphorylation (S727), while STAT3 knock‐down by siRNA reduced Pam3CSK4 induced TF expression. These results suggest that Pam3CSK4 induced TF expression is regulated by the JAK2–STAT3 signalin...
Source: Journal of Cellular Biochemistry - April 16, 2013 Category: Biochemistry Authors: Dae‐Weon Park, Ji Hyo Lyu, Jin‐Sik Kim, Haemin Chin, Yoe‐Sik Bae, Suk‐Hwan Baek Tags: Article Source Type: research

The RhoA pathway mediates MMP‐2 and MMP‐9‐independent invasive behavior in a triple‐negative breast cancer cell line
Abstract Breast cancer is a heterogeneous disease that varies in its biology and response to therapy. A foremost threat to patients is tumor invasion and metastasis, with the greatest risk among patients diagnosed with triple‐negative and/or basal‐like breast cancers. A greater understanding of the molecular mechanisms underlying cancer cell spreading is needed as 90% of cancer‐associated deaths result from metastasis. We previously demonstrated that the Tamoxifen‐selected, MCF‐7 derivative, TMX2‐28, lacks expression of estrogen receptor α (ERα) and is highly invasive, yet maintains an epithelial morphology. ...
Source: Journal of Cellular Biochemistry - April 16, 2013 Category: Biochemistry Authors: Katerina D. Fagan‐Solis, Sallie Smith Schneider, Brian T. Pentecost, Brook A. Bentley, Christopher N. Otis, John F. Gierthy, Kathleen F. Arcaro Tags: Article Source Type: research

S6 Kinase 2 is bound to chromatin‐nuclear matrix cellular fractions and is able to phosphorylate histone H3 at threonine 45 in vitro and in vivo
Abstract The activity of S6 kinasesis highly induced in cancer cells highlighting an essential role in carcinogenesis. The S6K family has two members: S6K1 and S6K2 which bear common as well as distinct features. In an attempt to identify S6K2 unique sequence features compared to S6K1, we applied extensive bioinformatic analysis and motif search approaches. Interestingly, we identified a number of protein signatures which are present in proteins directly connected to chromatin and/or involved in transcription regulation including three high mobility group proteins signatures. Using chromatin binding assay, we biochemically...
Source: Journal of Cellular Biochemistry - April 5, 2013 Category: Biochemistry Authors: Heba M.S. Ismail, Mahmoud Khalil, Marwa Dawoud Tags: Article Source Type: research

Dysregulation of BAG‐1 in hepatocellular carcinoma predicts patient outcome and mediates increased resistance to doxorubicin‐induced apoptosis via NF‐κB pathway
ABSTRACT Bcl‐2‐associated athanogene‐1 (BAG‐1) is a multifunctional anti‐apoptotic protein which regulates an array of cellular processes, including apoptosis, signaling, proliferation, transcription, and cell motility and has been reported to be over‐expressed in a number of human malignancies. To investigate the possible involvement of BAG‐1 in tumorigenesis of hepatocellular carcinoma (HCC), we performed Western blot analysis in eight paired samples of HCC and adjacent peritumoral tissues and immunohistochemistry in 65 paraffin sections of HCC, which both showed an enhanced expression of nuclear BAG‐1 is...
Source: Journal of Cellular Biochemistry - April 3, 2013 Category: Biochemistry Authors: Wenkai Ni, Buyou Chen, Guoxiong Zhou, Cuihua Lu, Mingbing Xiao, Chengqi Guan, Yixing Zhang, Song He, Aiguo Shen, Runzhou Ni Tags: Article Source Type: research

Gremlin Aggravates Hyperglycemia‐Induced Podocyte Injury by a TGFβ/Smad Dependent Signaling Pathway
In conclusion, gremlin was clearly elevated in high glucose cultured mouse podocytes, and likely employed endogenous canonical TGFβ1/Smad signaling to induce podocyte injury. Knockdown gremlin1 by siRNA may be clinically useful in the attenuation of podocyte injury. J. Cell. Biochem. © 2013 Wiley Periodicals, Inc.
Source: Journal of Cellular Biochemistry - March 31, 2013 Category: Biochemistry Authors: Guiying Li, Ying Li, Shuxia Liu, Yonghong Shi, Yanqing Chi, Guijing Liu, Tieying Shan Tags: Article Source Type: research

SOCS‐1/3 participation in FGF‐2 signaling to modulate RANK ligand expression in Paget's disease of bone
In this study, we hypothesized that FGF‐2 modulates suppressors of cytokine signaling (SOCS) to induce RANKL expression in PDB. We identified increased levels of SOCS‐1/3 mRNA expression in bone marrow mononuclear cells derived from patients with PDB compared to normal subjects. Interestingly, conditioned media obtained from MVNP transduced osteoclast progenitor cells significantly increased SOCS‐1/3 mRNA expression in stromal/preosteoblast cells. We next examined if SOCS participates in FGF‐2 signaling to modulate RANKL gene expression. We showed that FGF‐2 stimulation significantly increased SOCS‐1/3 expressi...
Source: Journal of Cellular Biochemistry - March 28, 2013 Category: Biochemistry Authors: Kumaran Sundaram, Joseph Senn, Sakamuri V. Reddy Tags: Article Source Type: research

Ceramide induces COX‐2‐dependent induction of apoptosis in human ovarian cancer OVCAR‐3 cells by mechanisms that partially overlap with actions of resveratrol
Abstract Ceramide is a member of the sphingolipid family of bioactive molecules demonstrated to have profound, diverse biological activities. Ceramide is a potential chemotherapeutic agent via the induction of apoptosis. Exposure to ceramide activates extracellular‐signal‐regulated kinases (ERK)1/2‐ and p38 kinase‐dependent apoptosis in human ovarian cancer OVCAR‐3 cells, concomitant with an increase in the expression of COX‐2 and p53 phosphorylation. Blockade of cyclooxygenase‐2 (COX‐2) activity by siRNA or NS398 correspondingly inhibited ceramide‐induced p53 Ser‐15 phosphorylation and apoptosis; thus ...
Source: Journal of Cellular Biochemistry - March 13, 2013 Category: Biochemistry Authors: Hung‐Yun Lin, Dominique Delmas, Ole Vang, Tze‐Chen Hsieh, Sharon Lin, Guei‐Yun Cheng, Hsiao‐Ling Chiang, Chiao En Chen, Heng‐Yuan Tang, Dana R. Crawford, Jacqueline Whang‐Peng, Jaulang Hwang, Leroy F. Liu, Joseph M. Wu Tags: Article Source Type: research

3,4‐dimethoxystilbene, a resveratrol derivative with anti‐angiogenic effect, induces both macroautophagy and apoptosis in endothelial cells
Abstract Angiogenesis plays an important role in many pathological processes. Identification of novel anti‐angiogenic agents will provide new insights into the mechanisms for angiogenesis as well as potential lead compounds for developing new drugs. In the present study, a series of resveratrol methylated derivatives have been synthesized and screened. We found trans‐3,4‐dimethoxystilbene (3,4‐DMS) with the fullest potential to develop as an anti‐angiogenic agent. In vitro and in vivo analyses suggested that 3,4‐DMS could effectively inhibit endothelial cell proliferation, migration, tube formation, and endogen...
Source: Journal of Cellular Biochemistry - January 22, 2013 Category: Biochemistry Authors: Lu Zhang, HongJuan Jing, LiuQing Cui, HuanQing Li, Bo Zhou, GuangZhou Zhou, Fang Dai Tags: Article Source Type: research

HDAC inhibitor DWP0016 activates p53 transcription and acetylation to inhibit cell growth in U251 glioblastoma cells
Abstract Here we report a hydroacid named DWP0016, which exhibited HDAC inhibition and induced p53 acetylation in U251 glioblastoma cells. DWP0016 effectively inhibited the cell growth of U251 cells and other 4 carcinoma cell lines but did not affect the normal cells. Cell cycle distribution analysis showed DWP0016 arrested at G1 phase cell cycle dose‐dependently in U251 cells. DWP0016 induced caspase‐dependent and independent apoptosis in U251 cells, which was identified by flow cytometry analysis, caspases activity analysis, western blotting assay and caspases inhibition. Mechanisms research suggested that DWP0016 ac...
Source: Journal of Cellular Biochemistry - January 7, 2013 Category: Biochemistry Authors: Hui Jin, Lei Liang, Li‐Feng Liu, Wei‐Ping Deng, Jian‐Wen Liu Tags: Article Source Type: research