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siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells
Methods Mol Biol. 2022;2550:113-121. doi: 10.1007/978-1-0716-2593-4_15.ABSTRACTSuspension primary cultures of rat pineal cells have been used for decades to determine biochemical regulatory mechanisms of pineal melatonin synthesis, but more recently, RNA interference technology has made the study of the role of specific genes in this melatonin-proficient model system possible. We here present a protocol for preparing rat pineal cell cultures and efficiently knock down gene expression by use of synthetic siRNA.PMID:36180684 | DOI:10.1007/978-1-0716-2593-4_15
Source: Mol Biol Cell - September 30, 2022 Category: Molecular Biology Authors: Aurea Susana Blancas-Velazquez Martin Fredensborg Rath Source Type: research

Melatonin Sensitizes OVCAR-3 Cells to Cisplatin through Suppression of PI3K/Akt Pathway
This study examined the effect of melatonin on oxidative stress, expression of pro-apoptotic protein, anti-apoptotic proteins, and the activity of the PI3K/Akt signaling pathway in the human ovarian cancer cell line (OVCAR3). OVCAR3 cells were treated with cisplatin, melatonin, cisplatin + melatonin, and siRNA Akt. Reactive oxygen species levels were assessed. The expression of the proteins was determined by Western blot. Melatonin administration significantly increased intracellular ROS generation, the cleavage of caspase 3 and decreased phosphorylation of Akt. Combination therapy of cisplatin and melatonin increases apop...
Source: Cellular and Molecular Biology - August 21, 2022 Category: Molecular Biology Authors: Sahar Baghal-Sadriforoush Morteza Bagheri Isa Abdi Rad Fattah Sotoodehnejadnematalahi Source Type: research

Melatonin ‑induced miR‑181c‑5p enhances osteogenic differentiation and mineralization of human jawbone‑derived osteoblastic cells.
In conclusion, MEL‑induced expression of miR‑181c‑5p enhanced osteogenic differentiation and calcification of hOB cells. PMID: 32945514 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - September 20, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Melatonin attenuates doxorubicin-induced cardiotoxicity through preservation of YAP expression.
In this study, we examined the possible protective effects of melatonin on doxorubicin-induced cardiotoxicity and explored the underlying mechanisms related to this process. We found that in vitro doxorubicin treatment significantly decreased H9c2 cell viability and induced apoptosis as manifested by increased TUNEL-positive cells, down-regulation of anti-apoptotic protein Bcl-2, as well as up-regulation of pro-apoptotic protein Bax. This was associated with increased reactive oxygen species (ROS) levels and decreased mitochondrial membrane potentials (MMP). In vivo, five weeks of doxorubicin treatment significantly decrea...
Source: J Cell Mol Med - February 17, 2020 Category: Molecular Biology Authors: Li HR, Wang C, Sun P, Liu DD, Du GQ, Tian JW Tags: J Cell Mol Med Source Type: research

Melatonin protects H9c2 cells against ischemia/reperfusion ‑induced apoptosis and oxidative stress via activation of the Nrf2 signaling pathway.
Melatonin protects H9c2 cells against ischemia/reperfusion‑induced apoptosis and oxidative stress via activation of the Nrf2 signaling pathway. Mol Med Rep. 2018 Jul 24;: Authors: Zhang Y, Qiao B, Gao F, Wang H, Miao S, Zhao H Abstract Melatonin can protect against cardiac ischemia/reperfusion (I/R) injury in models in vitro and in vivo by regulating oxidative stress and apoptosis; however, the precise molecular mechanisms involved remain unclear. Nuclear factor erythroid 2‑related factor 2 (Nrf2) is a transcription factor, which has been associated with the regulation of oxidative stress by tran...
Source: Molecular Medicine Reports - August 3, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Melatonin protects diabetic heart against ischemia-reperfusion injury, role of membrane receptor-dependent cGMP-PKG activation
This study was designed to determine the role of melatonin membrane receptors in melatonin's cardioprotective actions against diabetic MI/R injury with a focus on cGMP and its downstream effector PKG. Streptozotocin-induced diabetic Sprague-Dawley rats and high-glucose medium-incubated H9c2 cardiomyoblasts were utilized to determine the effects of melatonin against MI/R injury. Melatonin treatment preserved cardiac function and reduced oxidative damage and apoptosis. Additionally, melatonin increased intracellular cGMP level, PKGIα expression, p-VASP/VASP ratio and further modulated myocardial Nrf-2-HO-1 and MAPK signalin...
Source: Biochimica et Biophysica Acta (BBA) Molecular Basis of Disease - December 6, 2017 Category: Molecular Biology Source Type: research