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Specialty: Genetics & Stem Cells
Cancer: Melanoma

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Total 18 results found since Jan 2013.

GSE160638 MYC induces immunotherapy and interferon- γ resistance through downregulation of JAK2
Contributors : Ettai Markovits ; Ran Elkon ; Michal J Besser ; Gal MarkelSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensImmunotherapy revolutionized the treatment of advanced melanoma. As the pathways mediating resistance to immunotherapy are largely unknown, we conducted transcriptome profiling of pre-immunotherapy tumor biopsies from melanoma patients that received PD-1 blockade (n=36) or adoptive cell therapy with tumor infiltrating lymphocytes (n=37). We identified two melanoma-intrinsic mutually exclusive gene programs, which are controlled by interferon- γ and MYC, and determi...
Source: GEO: Gene Expression Omnibus - April 24, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Gallic Acid has an inhibitory effect on skin squamous cell carcinoma and acts on the heat shock protein HSP90AB1
Gene. 2022 Nov 11:147041. doi: 10.1016/j.gene.2022.147041. Online ahead of print.ABSTRACTDifferences in the features of aggressiveness of non-melanoma skin cancer (NMSC) subtypes, between basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) are relevant characteristics. Comparing the characteristics between NMSC subtypes might help identify molecules associated with cancer metastasis and invasion. Considering these facts, the current study aimed to identify a molecular target for inhibiting skin cancer metastasis and invasion. Proteomic analysis suggested that heat shock protein 90 kDa, alpha, class B member 1 (HSP...
Source: Gene - November 14, 2022 Category: Genetics & Stem Cells Authors: Sabrina Ferreira de Jesus Marcela Gon çalves de Souza Lorena Dos Reis Pereira Queiroz Daniela Paola Santos de Paula Angeliny Tamiarana Lima Tabosa Wislene Sarajane Moreira Alves Luiz Henrique da Silveira Andr é Teixeira da Silva Ferreira Ozires Jos é D Source Type: research

GSE131048 PERK signaling through C/EBP δ contributes to ER stress-induced expression of immunomodulatory and tumor promoting chemokines by cancer cells.
Contributors : Namratha Sheshadri ; Esta SterneckSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensExpression of the transcription factor CEBPD is induced at early stages of the endoplasmic reticulum (ER) stress response. In order to identify the genes modulated by CEBPD during the ER stress response, we transiently silenced CEBPD expression in MDA-MB-435 melanoma cells and isolated mRNA at 6 h of treatment with Thapsigargin or DMSO control. As control, cells were transfected with two different control siRNA oligonucleotides. Results provide insights into which genes are modulated by CE...
Source: GEO: Gene Expression Omnibus - November 1, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE115791 Transcriptome of primary and metastatic human melanoma with silenced STAT3
Contributors : Bozena Kaminska ; Dorota W Kulesza ; Kavita Ramji ; Jakub MieczkowskiSeries Type : Expression profiling by arrayOrganism : Homo sapiensPrimary and metastatic human melanoma cells were transfected with control siRNA or siSTAT3 to silence its expression. The global gene expression was evaluated after 48 h and related to gene levels in cells transfected with siRNA control.
Source: GEO: Gene Expression Omnibus - June 14, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

GSE167945 IRF4 genome wide binding and transciptomic profile in 501Mel melanoma cell line
Contributors : Ramile Dilshat ; Berglind O Einarsd óttirSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensTo map genome wide binding sites of IRF4, we performed ChIP-seq against eGFP tagged IRF4 in human melanoma cells. To assess whether IRF4 binding influences its target gene expression, RNA-seq was performed after siRNA mediated depletion of IRF4 in 501Mel cells. Subsequently, target genes of IRF4 obtained from ChIP-seq and RNA-seq studies were used to interrogate melanocyte meQTL and identified 131 candidate downstre...
Source: GEO: Gene Expression Omnibus - March 2, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Homo sapiens Source Type: research

GSE165112 Interferon-induced transmembrane protein 1 (IFITM1) inhibits  Varicella-Zoster Virus Replication
Contributors : Seong K Kim ; Akhalesh K Shakya ; Dennis J O'CallaghanSeries Type : Expression profiling by arrayOrganism : Homo sapiensHosts have evolved numerous mechanisms to prevent primary viral infections. Interferon signaling is an important host defense mechanism against primary infection. Interferon gamma (IFN- γ) is a potent cytokine produced following primary varicella-zoster virus (VZV) infection. Furthermore, VZV reactivation correlates with a decline in IFN-γ-producing immune cells. Our previous results showed that pretreatment with 20 ng/ml of IFN-γ completely inhibited VZV replication in lung fib roblast ...
Source: GEO: Gene Expression Omnibus - January 20, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

GSE163646 MITF reprograms the extracellular matrix and focal adhesion in melanoma
Contributor : Ramile DilshatSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo assess the effects of permanent loss of MITF in melanoma cells, we used the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 technique to generate MITF knockout (KO) cell lines in the human hypo-tetraploid SkMel28 melanoma cell line (containing four copies of MITF). We targeted exon 6 (containing the DNA binding domain) of MITF the resulting isogenic cell line is hereafter referred to as ΔMITF-X6. We also performed siRNA mediated transient knock down of MITF along with control siCTRL...
Source: GEO: Gene Expression Omnibus - December 22, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE145101 Transcriptome profiling of WM3248 melanoma cells following PGC1-alpha knockdown
Contributors : Iman Osman ; Keith GilesSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensPGC1-alpha is a transcriptional coactivator that controls diverse cellular processes, including metabolism. PGC1-alpha expression is increased in invasive primary melanoma. To understand the role of PGC1-alpha in the invasive growth of primary melanoma, we generated transcriptional (RNA-Seq) profiles of WM3248 primary melanoma cells that were transfected with either negative control or PGC1-alpha siRNA.
Source: GEO: Gene Expression Omnibus - February 12, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE115385 Transcriptomal analysis of the impact of ASAH1 inhibition on skin metastatic melanoma cells
Contributors : J Leclerc ; N Nottet ; C Bertolotto ; R BallottiSeries Type : Expression profiling by arrayOrganism : Homo sapiensTo assess the impact of ASAH1 inhibition skin metastatic melanoma, we transfected three different human skin metastatic melanoma cell lines (501Mel, WM3912 and WM8) with a siRNA designed against ASAH1 (50nM) or a siRNA control (50nM) for 48h.
Source: GEO: Gene Expression Omnibus - December 3, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

GSE92764 LPAR signaling activated in neural crest stem cells promotes melanoma cell growth, invasion and therapy resistance HumanHT-12 V4.0
Contributor : Liu JianglanSeries Type : Expression profiling by arrayOrganism : Homo sapiensLPAR1 (lysophosphatidic acid receptor 1) is identified in targeted siRNA screens which is required for the survival and maintenance of nueral crest stem cells (NCSC) as well as the growth and invasion of melanoma cells. To gain mechanistic insights into how LPAR signaling modulates melanoma cell growth, We conducted an Illumina genome-wide gene expression microarray experiment to profile melanoma cells treated with the autotaxin inhibitor, HA130. Melanoma cells treated with vehicle control,DMSO is included as a control.
Source: GEO: Gene Expression Omnibus - December 1, 2017 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

GSE79112 Gene expression profile using RNA-seq in WC00060 or SR-0788 cells transfected with siRNA for KPC1 or control
Contributors : Yuuki Iida ; Sandy Hsu ; Nellie Nelson ; Matthew P Salomon ; Dave S HoonSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensMelanoma is a highly aggressive cancer with increasing incidence rates and a poor survival, particularly in patients with AJCC stage IV and advanced stage III. Deregulation of NF-kB is linked to different pathological states, including melanoma. To identify the involvement of NF-kB pathway regulation in melanoma progression, we manipulated NF-kB pathway activation and profiled gene expression using RNA-sequencing.
Source: GEO: Gene Expression Omnibus - September 1, 2017 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

A ‘synthetic-sickness’ screen for senescence re-engagement targets in mutant cancer backgrounds
by Claire J. Cairney, Lauren S. Godwin, Alan E. Bilsland, Sharon Burns, Katrina H. Stevenson, Lynn McGarry, John Revie, Jon D. Moore, Ceri M. Wiggins, Rebecca S. Collinson, Clare Mudd, Elpida Tsonou, Mahito Sadaie, Dorothy C. Bennett, Masashi Narita, Christopher J. Torrance, W. Nicol Keith Senescence is a universal barrier to immortalisation and tumorigenesis. As such, interest in the use of senescence-induction in a therapeutic context has been gaining momentum in the past few years; however, senescence and immortalisation remain underserved areas for drug discovery owing to a lack of robust senescence inducing agents an...
Source: PLoS Genetics - August 14, 2017 Category: Genetics & Stem Cells Authors: Claire J. Cairney Source Type: research

GSE58199 Sema6A and Mical1 control cell growth and survival of BRAFV600E human melanomas
Contributors : L De Cecco ; A AnichiniSeries Type : Expression profiling by arrayOrganism : Homo sapiensTherapeutic targeting of BRAFV600Eand of MEK has shown a significant impact on progression-free and overall survival in advanced melanoma, but only a fraction of patients benefit from these treatments, suggesting that additional signaling pathways involved in melanoma growth/survival need to be identified. To this end, we used whole genome microarray analysis to identify differentially expressed genes in a set of neoplastic clones, isolated from a single melanoma metastasis, and characterized by mututally exclusive expre...
Source: GEO: Gene Expression Omnibus - April 19, 2016 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

High expression of Mcl‐1L via the MEK‐ERK‐phospho‐STAT3 (Ser727) pathway protects melanocytes and melanoma from UVB‐induced apoptosis
In this study, we characterized the involvement of Mcl‐1L in the regulation of UVB‐induced apoptosis in melanocytes and in melanoma cells. In melanocytes, apoptosis was not evident at 24 h after UVB irradiation. The Mcl‐1L expression increased after UVB irradiation, and the high Mcl‐1L expression continued for at least 24 h. This UVB‐dependent increase in Mcl‐1L was mediated by the MEK‐ERK‐pS‐STAT3 (STAT3 phosphorylated at Ser727) pathway. The Ser727 phosphorylation facilitated nuclear localization of STAT3. In melanoma cells, the expression levels of Mcl‐1L varied depending on the cell line. WM39 mel...
Source: Genes to Cells - January 1, 2016 Category: Genetics & Stem Cells Authors: Takeshi Fukumoto, Tetsushi Iwasaki, Taro Okada, Takanori Hashimoto, Youbin Moon, Masanobu Sakaguchi, Yasuo Fukami, Chikako Nishigori, Masahiro Oka Tags: Original Article Source Type: research