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Kmt2c modulates migration and invasion processes in osteosarcoma cell lines
In this study we investigated the role of KMT2C (a chromatin-modifying and remodelling protein) in osteosarcoma progression through cell migration and invasion assays in osteosarcoma primary and metastatic cell lines. Wound healing and transwell assays were used to detect changes of cell migration and matrigel assay was used to evaluate changes of cell invasion in primary and metastatic osteosarcoma cell lines after KMT2C siRNA transfection. We found that primary osteosarcoma cell lines showed the highest capacity of migration before mRNA KMT2C silencing and the highest capacity of invasion after mRNA KMT2C silencing; on t...
Source: Pathology Research and Practice - July 13, 2019 Category: Pathology Source Type: research

Upregulation of miR‑95-3p inhibits growth of osteosarcoma by targeting HDGF
Publication date: Available online 10 June 2019Source: Pathology - Research and PracticeAuthor(s): Xiwei Liu, Wei Ma, Jianbing Ma, Lin Xiao, Dingjun HaoAbstractOsteosarcoma is the most common bone malignancy and miR-95-3p plays an important role in multiple cancers. The purpose of this study was to explore the effect and potential mechanism of miR-95-3p on the growth of osteosarcoma. In vitro, the osteosarcoma cell lines, SAOS-2 and U2OS cells, were transfected with miR-95-agomir to assess the role of miR-95-3p in proliferation and apoptosis of osteosarcoma cells. We determined that overexpression of miR-95-3p significantl...
Source: Pathology Research and Practice - June 11, 2019 Category: Pathology Source Type: research

Sox9 regulates hyperexpression of Wnt1 and Fzd1 in human osteosarcoma tissues and cells.
This study investigates the expression of Sox9, Wnt1 and Fzd1 in human osteosarcoma tissues and cells and the role of Sox9 in the proliferation of human osteosarcoma cells. Immunohistochemical analyses for Sox9, Wnt1, Fzd1, and Ki-67 proteins were performed in human primary osteosarcoma tissues from 48 patients. The small interfering RNA (siRNA) of Sox9 was transfected into human osteosarcoma MG63 cells. At 24 and 48 h after transfection with Sox9 siRNA, the expression of Wnt1 and Fzd1 was analyzed by RT-qPCR, Western blot, and immunofluorescence techniques. Cell proliferation was assayed by CCK-8 method, and Ki-67 protein...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Liu H, Chen Y, Zhou F, Jie L, Pu L, Ju J, Li F, Dai Z, Wang X, Zhou S Tags: Int J Clin Exp Pathol Source Type: research

Modulation of insulin /IGFs pathways by sirtuin-7 inhibition in drug- induced chemoreistance
Conclusion: Our data demonstrate that stress-induced Sirt7 inhibition significantly increases stress resistance and modulates insulin/IGF-1 signaling pathways. More importantly, this study links Sir2 family proteins to insulin/IGF signaling in drug-induced stress resistance in neoplasia.Virtual Slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1135426681234493
Source: Diagnostic Pathology - May 22, 2014 Category: Pathology Authors: Ahmad AljadaAyman SalehSalem Al Suwaidan Source Type: research