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Total 135484 results found since Jan 2013.

Regulation of Pro-Inflammatory Macrophage Polarization via Lipid Nanoparticles Mediated Delivery of Anti-Prostaglandin-E2 siRNA
Curr Issues Mol Biol. 2022 Dec 20;45(1):1-11. doi: 10.3390/cimb45010001.ABSTRACTPro-inflammatory macrophage polarization is crucial in acute inflammatory diseases like Acute lung injury (ALI), and acute respiratory distress syndrome (ARDS). Prostaglandin E2 (PGE2) is believed to promote inflammation in such cases. Therefore, our study aimed to deliver anti-prostaglandin E synthase 2 small interfering RNA antibodies (anti-PGE2-siRNA) through lipid nanoparticles (LNPs) in RAW264.7 (The murine macrophage cell line) to find a possible cure to the acute inflammatory diseases. LNPs were synthesized by using thin layer evaporatio...
Source: Mol Biol Cell - January 20, 2023 Category: Molecular Biology Authors: Ahmad Almatroudi Mohammed A Alsahli Mansoor Ali Syed Amjad Ali Khan Arshad Husain Rahmani Source Type: research

Effect of the combination of cationic lipid and phospholipid on gene-knockdown using siRNA lipoplexes in breast tumor cells and mouse lungs
Mol Med Rep. 2023 Oct;28(4):180. doi: 10.3892/mmr.2023.13067. Epub 2023 Aug 18.ABSTRACTPreviously, using three types of cationic lipids, the effect of phospholipids in liposomal formulations on gene-knockdown efficacy was determined after in vitro and in vivo transfection with small interfering RNA (siRNA)/cationic liposome complexes (siRNA lipoplexes) containing various cationic lipids and phospholipids. In the present study, six other types of cationic lipids, namely N,N-dimethyl-N-tetradecyltetradecan-1-aminium bromide, N-hexadecyl-N,N-dimethylhexadecan-1-aminium bromide (DC-1-16), 2-[bis{2-(tetradecanoyloxy)ethyl}amino...
Source: Molecular Medicine - August 18, 2023 Category: Molecular Biology Authors: Yoshiyuki Hattori Min Tang Aya Aoki Momoka Ezaki Hana Sakai Kei-Ichi Ozaki Source Type: research

Effects of sterol derivatives in cationic liposomes on  biodistribution and gene-knockdown in the lungs of mice systemically injected with siRNA lipoplexes
Mol Med Rep. 2021 Aug;24(2):598. doi: 10.3892/mmr.2021.12237. Epub 2021 Jun 24.ABSTRACTCationic liposomes can be intravenously injected to deliver short interfering (si)RNAs into the lungs. The present study investigated the effects of sterol derivatives in systemically injected siRNA/cationic liposome complexes (siRNA lipoplexes) on gene‑knockdown in the lungs of mice. Cationic liposomes composed of 1,2‑dioleoyl‑3‑trimethylammonium‑propane or dimethyldioctadecylammonium bromide (DDAB) were prepared as a cationic lipid, with sterol derivatives such as cholesterol (Chol), β‑sitosterol, ergosterol (Ergo) or stig...
Source: Molecular Medicine - June 24, 2021 Category: Molecular Biology Authors: Yoshiyuki Hattori Hiromu Saito Teruaki Oku Kei-Ichi Ozaki Source Type: research

A New Approach of Delivering siRNA to the Cornea and its Application for Inhibiting Herpes Simplex Keratitis.
Abstract Small interfering RNA (siRNA) is a potential agent for the treatment of ocular surface diseases. Previous studies delivered siRNA by directly injecting siRNA into cornea or conjunctiva. In the present study we sought to explore an alternative approach to deliver siRNA into mouse cornea via eye drops that contains cy3-labeled siRNA (cy3-siRNA) and different cationic complexing agents and to evaluate the effects of siRNA targeting HSV-1 ICP4 gene (ICP4-siRNA) on mouse herpes simplex keratitis (HSK). Cy3-siRNA was mixed with Lipofectamine 2000, EntransterTM-in vivo, polyethyleneimine (PEI) or PEO-PPO-PEO pol...
Source: Current Molecular Medicine - October 21, 2014 Category: Molecular Biology Authors: Li Z, Duan F, Lin L, Huang Q, Wu K Tags: Curr Mol Med Source Type: research

Co-delivery of Doxorubicin and Bmi1 siRNA by Folate Receptor Targeted Liposomes Exhibits Enhanced Anti-Tumor Effects in vitro and in vivo
Bmi1 gene overexpression is found in various human tumors and has been shown as a potential target for gene treatment. However, siRNA-based treatments targeting Bmi1 gene have been restricted to limited delivery, low bioavailability and hence relatively reduced efficacy. To overcome these barriers, we developed a folate receptor targeted co-delivery system folate-doxorubicin/Bmi1 siRNA liposome (FA-DOX/siRNA-L). The FA-DOX/siRNA-L was prepared through electrostatic interaction between folate doxorubicin liposome (FA-DOX-L) and Bmi1 siRNA. In vitro and in vivo studies showed that FA-DOX/siRNA-L inhibited tumor growth by com...
Source: Theranostics - November 15, 2014 Category: Molecular Biology Authors: Tan Yang, Bin Li, Shibo Qi, Yong Liu, Yongkang Gai, Peng Ye, Guang Yang, Wendian Zhang, Peng Zhang, Xingxing He, Weijie Li, Zhiping Zhang, Guangya Xiang, Chuanrui Xu Tags: Research Paper Source Type: research

Intrathecal injection of phosphodiesterase 4B-specific siRNA attenuates neuropathic pain in rats with L5 spinal nerve ligation.
Authors: Ji Q, Di Y, He X, Liu Q, Liu J, Li W, Zhang L Abstract Phosphodiesterase 4 (PDE4) is an adenosine cyclic 3,5-monophosphate-specific degradative enzyme, which is closely associated with the inflammatory response. Among its four subtypes (A‑D), it remains unclear which one exerts suppressive effects on inflammation and reduces neuropathic pain. The present study aimed to examine the modulation of neuroinflammation by PDE4 subtypes in the spinal cord of a rat model of L5 spinal nerve ligation (SNL)‑induced neuropathic pain. The expression levels of PDE4A‑D were measured in the lumbar spinal cords of naÃ...
Source: Molecular Medicine Reports - December 29, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Effect of pre ‑freezing and saccharide types in freeze‑drying of siRNA lipoplexes on gene‑silencing effects in the cells by reverse transfection.
Effect of pre‑freezing and saccharide types in freeze‑drying of siRNA lipoplexes on gene‑silencing effects in the cells by reverse transfection. Mol Med Rep. 2020 Aug 05;: Authors: Tang M, Hu S, Hattori Y Abstract Our previous study reported that reverse (Rev)‑transfection with small interfering RNA (siRNA)/cationic liposome complexes (siRNA lipoplexes) freeze‑dried in trehalose or sucrose solution resulted in high gene‑silencing activity in cells. The current study investigated whether pre‑freezing or saccharide types present during the freeze‑drying of siRNA lipoplexes affected geneâ€...
Source: Molecular Medicine Reports - September 20, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Effect of the combination of cationic lipid and phospholipid on gene-knockdown using siRNA lipoplexes in breast tumor cells and mouse lungs
Mol Med Rep. 2023 Oct;28(4):180. doi: 10.3892/mmr.2023.13067. Epub 2023 Aug 18.ABSTRACTPreviously, using three types of cationic lipids, the effect of phospholipids in liposomal formulations on gene-knockdown efficacy was determined after in vitro and in vivo transfection with small interfering RNA (siRNA)/cationic liposome complexes (siRNA lipoplexes) containing various cationic lipids and phospholipids. In the present study, six other types of cationic lipids, namely N,N-dimethyl-N-tetradecyltetradecan-1-aminium bromide, N-hexadecyl-N,N-dimethylhexadecan-1-aminium bromide (DC-1-16), 2-[bis{2-(tetradecanoyloxy)ethyl}amino...
Source: Molecular Medicine Reports - August 18, 2023 Category: Molecular Biology Authors: Yoshiyuki Hattori Min Tang Aya Aoki Momoka Ezaki Hana Sakai Kei-Ichi Ozaki Source Type: research