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Wharton's Jelly Mesenchymal Stem Cells-derived Exosomes and Imipenem in Combination Reduce Apoptosis and Inflammatory Responses in E.coli-infected HepG2 Cells
In this study, we investigated the immunomodulatory effect of Wharton's jelly MSC-derived exosomes in combination with imipenem on HepG2 cells infected with Escherichia col i.MSC-derived exosomes were separated from MSCs, which were isolated by flow cytometry. Scanning electron microscopy and dynamic light scanning were used to confirm the presence of exosomes. Quantitative real-time PCR, ELISA, and nitric oxide assay were used to assess the inflammatory response in the infected cells. Annexin-PI was used to measure the extent of apoptosis. The results showed that the combination of imipenem and MSC-derived exosomes were m...
Source: Iranian Journal of Allergy, Asthma and Immunology - July 13, 2022 Category: Allergy & Immunology Authors: Ali Hazrati Sara Soudi Seyed Mahmoud Hashemi Source Type: research
