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In-depth profiling of carbohydrate isomers in biological tissues by chemical derivatization-assisted mass spectrometry imaging
In this study, we propose a high-sensitive and isomer-specific imaging approach to visualize the spatial distributions of monosaccharide and disaccharide isomers by integrating chemical derivatization and matrix-assisted laser desorption/ionization tandem mass spectrometry imaging (MALDI-MS2I). 2-Pyridinecarbohydrazide (PYD) is developed as a novel derivatization reagent which can not only improves the MS sensitivity of carbohydrates, but also enables the identification and visualization of ketose and aldose monosaccharide isomers, as well as linkage pattern, monosaccharide composition and anomeric configuration disacchari...
Source: Analytica Chimica Acta - September 14, 2023 Category: Chemistry Authors: Lei Wang Yuhao Han Yaqi Zhang Haoyuan Geng Zihan Zhu Panpan Chen Xiaoqing Cui Xiao Wang Chenglong Sun Source Type: research
Total Energy Expenditure and Nutritional Intake in Continuous Multiday Ultramarathon Events
Int J Sport Nutr Exerc Metab. 2023 Sep 14:1-7. doi: 10.1123/ijsnem.2023-0063. Online ahead of print.ABSTRACTContinuous multiday ultramarathon competitions are increasingly popular and impose extreme energetic and nutritional demands on competitors. However, few data have been published on energy expenditure during these events. Here, we report doubly labeled water-derived measures of total energy expenditure (in kilocalories per day) and estimated physical activity level (PAL: total energy expenditure/basal metabolic rate) collected from five elite and subelite finishers (four males and one female, age 34.6 ± 4.9 years)-a...
Source: International Journal of Sport Nutrition and Exercise Metabolism - September 14, 2023 Category: Sports Medicine Authors: Andrew W Best Amanda McGrosky Zane Swanson Rebecca Rimbach Katie McConaughy Joe McConaughy Cara Ocobock Herman Pontzer Source Type: research
In-depth profiling of carbohydrate isomers in biological tissues by chemical derivatization-assisted mass spectrometry imaging
In this study, we propose a high-sensitive and isomer-specific imaging approach to visualize the spatial distributions of monosaccharide and disaccharide isomers by integrating chemical derivatization and matrix-assisted laser desorption/ionization tandem mass spectrometry imaging (MALDI-MS2I). 2-Pyridinecarbohydrazide (PYD) is developed as a novel derivatization reagent which can not only improves the MS sensitivity of carbohydrates, but also enables the identification and visualization of ketose and aldose monosaccharide isomers, as well as linkage pattern, monosaccharide composition and anomeric configuration disacchari...
Source: Analytica Chimica Acta - September 14, 2023 Category: Chemistry Authors: Lei Wang Yuhao Han Yaqi Zhang Haoyuan Geng Zihan Zhu Panpan Chen Xiaoqing Cui Xiao Wang Chenglong Sun Source Type: research
Optimizing carbohydrate intake from drugs in epileptic patients on a ketogenic diet
Nutr Hosp. 2023 Sep 1. doi: 10.20960/nh.04798. Online ahead of print.NO ABSTRACTPMID:37705443 | DOI:10.20960/nh.04798
Source: Nutricion Hospitalaria - September 14, 2023 Category: Nutrition Authors: Javier Coraz ón Villanueva Mar ía de la Torre Ortíz Virg ínia Puebla García Jos é Manuel Martínez Sesmero Source Type: research
The origins of the obesity epidemic may be further back than we thought
Over the past 50 years, worldwide obesity rates have tripled, creating a public health crisis so widespread and damaging that it is sometimes referred to as an epidemic. Most accounts put the roots of the problem firmly in the modern age. But could it have been brewing since before World War II?
That’s one provocative conclusion of a study published today in
Science Advances
that purports to
push the obesity epidemic’s origin back to as early as the 1930s
. Historical measurements from hundreds of thousands of Danish youth show that in the decades before the problem was officially recognized, the heavi...
Source: Science of Aging Knowledge Environment - September 13, 2023 Category: Geriatrics Source Type: research
The < em > Ustilago maydis < /em > AA10 LPMO is active on fungal cell wall chitin
In this study, we successfully produced the catalytic domain of this LPMO (UmAA10_cd) in Pichia pastoris and carried out its biochemical characterization. Our results show that UmAA10_cd oxidatively cleaves α- and β-chitin with C1 regioselectivity and boosts chitin hydrolysis by a GH18 chitinase from U. maydis (UmGH18A). Using a biologically relevant substrate, we show that UmAA10_cd exhibits enzymatic activity on U. maydis fungal cell wall chitin and promotes its hydrolysis by UmGH18A. These results represent an important step toward the understanding of the role of LPMOs in the fungal cell wall remodeling process durin...
Source: Applied and Environmental Microbiology - September 13, 2023 Category: Microbiology Authors: Roseline Assiah Yao Jean-Lou Reyre Ketty C Tamburrini Mireille Haon Olivier Tranquet Akshay Nalubothula Saumashish Mukherjee Sophie Le Gall Sacha Grisel Sonia Longhi Jogi Madhuprakash Bastien Bissaro Jean-Guy Berrin Source Type: research
Linseed oil attenuates fatty liver disease in mice fed a high-carbohydrate diet
In conclusion, linseed oil impedes or reduces the liver injury progression induced by an HCD.PMID:37703111 | DOI:10.1590/1414-431X2023e12927
Source: Braz J Med Biol Res - September 13, 2023 Category: Research Authors: G Godoy C C O Bernardo L Casagrande M L M S érgio J N Zanoni J V C M Perles R Curi R B Bazotte Source Type: research
Contribution of school meals to the recommended nutrient and energy intake of children enrolled in the National Homegrown School Feeding Program in Zaria, Nigeria
CONCLUSION: The meals served through the NHSFP contributed at least 33% of the RNI for energy, protein, iron, calcium, sodium, vitamin A, and zinc. However, they could not meet the 33% of the RNI for carbohydrates, fiber, potassium, and vitamin C. Increasing the portion sizes and the diversity of the meals can address the suboptimal contribution of the meals to the RNI for carbohydrates, fiber, potassium, and vitamin C.PMID:37704525 | DOI:10.1016/j.arcped.2023.07.004
Source: Archives de Pediatrie - September 13, 2023 Category: Pediatrics Authors: Halliru Zailani Olumuyiwa Adeyemi Owolabi Abdullahi Balarabe Sallau Source Type: research
Linseed oil attenuates fatty liver disease in mice fed a high-carbohydrate diet
In conclusion, linseed oil impedes or reduces the liver injury progression induced by an HCD.PMID:37703111 | PMC:PMC10496762 | DOI:10.1590/1414-431X2023e12927
Source: Braz J Med Biol Res - September 13, 2023 Category: Research Authors: G Godoy C C O Bernardo L Casagrande M L M S érgio J N Zanoni J V C M Perles R Curi R B Bazotte Source Type: research
Contribution of school meals to the recommended nutrient and energy intake of children enrolled in the National Homegrown School Feeding Program in Zaria, Nigeria
CONCLUSION: The meals served through the NHSFP contributed at least 33% of the RNI for energy, protein, iron, calcium, sodium, vitamin A, and zinc. However, they could not meet the 33% of the RNI for carbohydrates, fiber, potassium, and vitamin C. Increasing the portion sizes and the diversity of the meals can address the suboptimal contribution of the meals to the RNI for carbohydrates, fiber, potassium, and vitamin C.PMID:37704525 | DOI:10.1016/j.arcped.2023.07.004
Source: Archives de Pediatrie - September 13, 2023 Category: Pediatrics Authors: Halliru Zailani Olumuyiwa Adeyemi Owolabi Abdullahi Balarabe Sallau Source Type: research
Biosolids application enhances the growth of Aloe vera plants and provides a sustainable practice for nutrient recirculation in agricultural soils
Environ Sci Pollut Res Int. 2023 Sep 13. doi: 10.1007/s11356-023-29763-4. Online ahead of print.ABSTRACTIn the present study, the fertilization potential of biosolids (sewage sludge; SS) for the cultivation of Aloe vera plants was investigated using block design. Pot experiments were conducted in this study using 50, 100, 150, and 200 g/kg of SS. Results showed that SS-fertilized soils significantly (p < 0.05) affected the proximate, biochemical, and heavy metal parameters of A. vera plants. In particular, the T4 treatment gave the best results with maximum plant height 62.21 ± 0.10 cm, number of leaves per plant 18.00...
Source: Environmental Science and Pollution Research International - September 13, 2023 Category: Environmental Health Authors: Arwa A Al-Huqail Pankaj Kumar Sonika Kumari Ebrahem M Eid Source Type: research
The < em > Ustilago maydis < /em > AA10 LPMO is active on fungal cell wall chitin
In this study, we successfully produced the catalytic domain of this LPMO (UmAA10_cd) in Pichia pastoris and carried out its biochemical characterization. Our results show that UmAA10_cd oxidatively cleaves α- and β-chitin with C1 regioselectivity and boosts chitin hydrolysis by a GH18 chitinase from U. maydis (UmGH18A). Using a biologically relevant substrate, we show that UmAA10_cd exhibits enzymatic activity on U. maydis fungal cell wall chitin and promotes its hydrolysis by UmGH18A. These results represent an important step toward the understanding of the role of LPMOs in the fungal cell wall remodeling process durin...
Source: Applied and Environmental Microbiology - September 13, 2023 Category: Microbiology Authors: Roseline Assiah Yao Jean-Lou Reyre Ketty C Tamburrini Mireille Haon Olivier Tranquet Akshay Nalubothula Saumashish Mukherjee Sophie Le Gall Sacha Grisel Sonia Longhi Jogi Madhuprakash Bastien Bissaro Jean-Guy Berrin Source Type: research
Biosolids application enhances the growth of Aloe vera plants and provides a sustainable practice for nutrient recirculation in agricultural soils
Environ Sci Pollut Res Int. 2023 Sep 13. doi: 10.1007/s11356-023-29763-4. Online ahead of print.ABSTRACTIn the present study, the fertilization potential of biosolids (sewage sludge; SS) for the cultivation of Aloe vera plants was investigated using block design. Pot experiments were conducted in this study using 50, 100, 150, and 200 g/kg of SS. Results showed that SS-fertilized soils significantly (p < 0.05) affected the proximate, biochemical, and heavy metal parameters of A. vera plants. In particular, the T4 treatment gave the best results with maximum plant height 62.21 ± 0.10 cm, number of leaves per plant 18.00...
Source: Environmental Science and Pollution Research International - September 13, 2023 Category: Environmental Health Authors: Arwa A Al-Huqail Pankaj Kumar Sonika Kumari Ebrahem M Eid Source Type: research
The < em > Ustilago maydis < /em > AA10 LPMO is active on fungal cell wall chitin
In this study, we successfully produced the catalytic domain of this LPMO (UmAA10_cd) in Pichia pastoris and carried out its biochemical characterization. Our results show that UmAA10_cd oxidatively cleaves α- and β-chitin with C1 regioselectivity and boosts chitin hydrolysis by a GH18 chitinase from U. maydis (UmGH18A). Using a biologically relevant substrate, we show that UmAA10_cd exhibits enzymatic activity on U. maydis fungal cell wall chitin and promotes its hydrolysis by UmGH18A. These results represent an important step toward the understanding of the role of LPMOs in the fungal cell wall remodeling process durin...
Source: Applied and Environmental Microbiology - September 13, 2023 Category: Microbiology Authors: Roseline Assiah Yao Jean-Lou Reyre Ketty C Tamburrini Mireille Haon Olivier Tranquet Akshay Nalubothula Saumashish Mukherjee Sophie Le Gall Sacha Grisel Sonia Longhi Jogi Madhuprakash Bastien Bissaro Jean-Guy Berrin Source Type: research
