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Specialty: Parasitology

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Total 22 results found since Jan 2013.

Development of siRNA mediated RNA interference and functional analysis of novel parasitic nematode-specific protein of Setaria digitata.
In this study, we developed an effective siRNA delivery method by microinjection and utilized the siRNAi tool to knockdown SDNP to study the phenotypic and cellular changes associated with the interference. qPCR analysis revealed, a significant reduction of SDNP transcript levels following siRNA microinjection into S. digitata adult worms. Similarly, immunohistochemical staining indicated a reduction of SDNP protein expression. Furthermore, worms treated with siRNA showed a significant reduction of microfilariae release together with embryonic lethality by arresting an early developmental stage compared to non-treated worm...
Source: Experimental Parasitology - February 12, 2018 Category: Parasitology Authors: Somarathne MBCL, Gunawardena YINS, Chandrasekharan NV, Dassanayake RS Tags: Exp Parasitol Source Type: research

RNA interference in Fasciola hepatica newly excysted juveniles: Long dsRNA induces more persistent silencing than siRNA
Publication date: October 2014 Source:Molecular and Biochemical Parasitology, Volume 197, Issues 1–2 Author(s): Nicolás Dell’Oca , Tatiana Basika , Ileana Corvo , Estela Castillo , Paul J. Brindley , Gabriel Rinaldi , Jose F. Tort In trematodes RNA interference is the current tool of choice for functional analysis of genes since classical reverse genetic approaches remain unavailable. Whereas this approach has been optimized in schistosomes, few reports are available for other trematodes, likely reflecting the difficulties in the establishment of the technology. Here we standardized conditions for RNAi in the liver ...
Source: Molecular and Biochemical Parasitology - November 17, 2014 Category: Parasitology Source Type: research

Inhibitory effects and analysis of RNA interference on thioredoxin glutathione reductase expression in Schistosoma japonicum.
Abstract Abstract Schistosomes infect around 280 million people worldwide. The worms survive in the veins of the final host, where thioredoxin glutathione reductase (TGR) activity helps the parasites to survive in the aerobic environment. In the present study, we synthesized 2 small interfering RNAs (siRNA S1 and S2) targeting the Schistosoma japonicum (Sj) TGR gene, and used them to knockdown the TGR gene. The knockdown effects of the siRNAs on SjTGR and the thioredoxin reductase (TrxR) activity of SjTGR were evaluated in vitro and in mice infected with schistosomes. The results of transfection with the siRNAs vi...
Source: The Journal of Parasitology - March 15, 2014 Category: Parasitology Authors: Han Y, Fu Z, Hong Y, Zhang M, Han H, Lu K, Yang J, Xiangrui LI, Lin J Tags: J Parasitol Source Type: research

Knocking down the expression of the molecular motors, myosin A, C and F genes in Toxoplasma gondii to decrease the parasite virulence
In conclusion, myosin proteins knock down proposes a promising therapeutic strategy to combat toxoplasmosis.PMID:37331576 | DOI:10.1016/j.exppara.2023.108565
Source: Experimental Parasitology - June 18, 2023 Category: Parasitology Authors: Tayebeh Zohrabi Mojtaba Azimi-Resketi Fereshteh Talaei Maryam Yaghoubi Mohamad Reza Ganjalikhany Farzaneh Mohamadi Farsani Abbasali Eskandarian Source Type: research

In vitro effects of Harmine against Echinococcus granulosus protoscoleces by stimulating DNA damage
In this study, we aim to evaluate the anti-parasitic efficacy and the mechanism of DNA damage of HM against E. granulosus. In vitro, the results indicated that, within two and three days of treatment, ABZ killed 30.4% and 35.3% of protoscoleces, whereas HM killed 52.7% and 100% of protoscoleces, respectively. Furthermore, the presence of abnormalities in the internal structure of protoscoleces was examined by ultrastructural images of TEM, and the result showed that there were scattered nucleoli and heterochromatin margination phenomenon by HM treatment. DNA damage of protoscoleces was examined by using the comet assay, an...
Source: Experimental Parasitology - June 7, 2021 Category: Parasitology Authors: Shuai Lu Limei Wen Yuehong Gong Chunyan Tian Huijing Gao Bei Chen Guodong L ü Jun Zhao Jianhua Wang Source Type: research

In vitro effects of harmine against Echinococcus granulosus protoscoleces by stimulating DNA damage
In this study, we aim to evaluate the anti-parasitic efficacy and the mechanism of DNA damage of HM against E. granulosus. In vitro, the results indicated that, within two and three days of treatment, ABZ killed 30.4% and 35.3% of protoscoleces, whereas HM killed 52.7% and 100% of protoscoleces, respectively. Furthermore, the presence of abnormalities in the internal structure of protoscoleces was examined by ultrastructural images of TEM, and the result showed that there were scattered nucleoli and heterochromatin margination phenomenon by HM treatment. DNA damage of protoscoleces was examined by using the comet assay, an...
Source: Experimental Parasitology - June 7, 2021 Category: Parasitology Authors: Shuai Lu Limei Wen Yuehong Gong Chunyan Tian Huijing Gao Bei Chen Guodong L ü Jun Zhao Jianhua Wang Source Type: research

Schistosoma japonicum translationally controlled tumor protein, which is associated with the development of female worms, as a target for control of schistosomiasis
In this study, the proteomic profiles of single-sex infected female (SF) worms and bisexual infected mature female (MF) worms of Schistosoma japonicum at 18, 21, 23 and 25 days p.i. were identified with isobaric tags for relative quantitation-coupled liquid chromatography-tandem mass spectrometry. Differentially expressed proteins (DEPs) were subsequently used for bioinformatic analysis. Six highly expressed DEPs in MF worms were selected and long-term interference with small interfering RNA (siRNA) was conducted to determine biological functions. SiRNA against S. japonicum translationally controlled tumor protein (SjTCTP)...
Source: International Journal for Parasitology - March 23, 2022 Category: Parasitology Authors: Haoran Zhong Yuqi Ren Fanglin Qin Xiaochun Li Ling Hou Shaopeng Gu Yamei Jin Source Type: research

Comparative proteomic profiles of Schistosoma japonicum male worms derived from single-sex and bisexual infections
In this study, the proteomic profiles of single-sex infected male (SM) worms and bisexual infected mated male (MM) worms of Schistosoma japonicum at 18, 21, 23, and 25 days p.i. were identified through data-independent acquisition. In total, 674 differentially expressed proteins (DEPs) were identified for the SM and MM worms at all four timepoints. Bioinformatic analysis demonstrated that most of the DEPs were involved in biosynthetic processes including locomotion, cell growth and death, cell motility, and metabolic processes such as protein metabolism and glucose metabolism. Schistosoma japonicum glycosyltransferase (SjG...
Source: International Journal for Parasitology - October 20, 2022 Category: Parasitology Authors: Haoran Zhong Luobin Wu Yuqi Ren Fanglin Qin Yamei Jin Source Type: research

The involvement of FAK and Src in the invasion of cardiomyocytes by Trypanosoma cruzi.
Abstract The activation of signaling pathways involving protein tyrosine kinases (PTKs) has been demonstrated during Trypanosoma cruzi invasion. Herein, we describe the participation of FAK/Src in the invasion of cardiomyocytes by T. cruzi. The treatment of cardiomyocytes with genistein, a PTK inhibitor, significantly reduced T. cruzi invasion. Also, PP1, a potent Src-family protein inhibitor, and PF573228, a specific FAK inhibitor, also inhibited T. cruzi entry; maximal inhibition was achieved at concentrations of 25μM PP1 (53% inhibition) and 40μM PF573228 (50% inhibition). The suppression of FAK expression in...
Source: Experimental Parasitology - February 25, 2014 Category: Parasitology Authors: de Melo TG, Tucci AR, Nogueira AR, Meirelles MD, Pereira MC Tags: Exp Parasitol Source Type: research

RNA interference, growth and differentiation appear normal in African trypanosomes lacking Tudor staphylococcal nuclease
Publication date: November 2010 Source:Molecular and Biochemical Parasitology, Volume 174, Issue 1 Author(s): Sam Alsford , Louise E. Kemp , Taemi Kawahara , David Horn Ribonucleases play important roles in the RNA interference (RNAi) pathway. The Dicer endonuclease converts double-stranded (ds)RNA into small interfering (si)RNA and the Slicer endonuclease, as a component of the RNA induced silencing complex (RISC), cleaves mRNA. Tudor staphylococcal nuclease (Tudor-SN) is another component of RISC in humans, flies and nematodes and is therefore implicated in the RNAi pathway. Here, we explore the potential role of Afric...
Source: Molecular and Biochemical Parasitology - November 3, 2014 Category: Parasitology Source Type: research