Proteomic analysis of Ascocotyle longa (Trematoda: Heterophyidae) metacercariae
Publication date: Available online 1 August 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Karina M. Rebello, Juliana N. Borges, André Teixeira, Jonas Perales, Cláudia P. Santos (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - August 1, 2020 Category: Parasitology Source Type: research

Editorial Board
Publication date: July 2020Source: Molecular and Biochemical Parasitology, Volume 238Author(s): (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - July 31, 2020 Category: Parasitology Source Type: research

A lysosomal hydrolase receptor, CPBF2, is associated with motility and invasion of the enteric protozoan parasite Entamoeba histolytica
Publication date: September 2020Source: Molecular and Biochemical Parasitology, Volume 239Author(s): Kumiko Nakada-Tsukui, Konomi Marumo, Tomoyoshi Nozaki (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - July 31, 2020 Category: Parasitology Source Type: research

p97 Inhibitor CB-5083 Blocks ERAD in Trypanosoma brucei
Publication date: Available online 28 July 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Paige Garrison, James D. Bangs (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - July 29, 2020 Category: Parasitology Source Type: research

A lysosomal hydrolase receptor, CPBF2, is associated motility and invasion of the enteric protozoan parasite Entamoeba histolytica
Publication date: Available online 21 July 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Kumiko Nakada-Tsukui, Konomi Marumo, Tomoyoshi Nozaki (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - July 22, 2020 Category: Parasitology Source Type: research

Direct and High-Throughput Assays for Human Cell Killing through Trogocytosis by Entamoeba histolytica
Publication date: Available online 17 July 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Akhila Bettadapur, Katherine S. Ralston (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - July 19, 2020 Category: Parasitology Source Type: research

A rapid approach for in locus overexpression of Trypanosoma brucei proteins
Publication date: Available online 16 July 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Hana Vachova, Glenda Alquicer, Miroslava Sedinova, Jana Sachova, Miluse Hradilova, Vladimir Varga (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - July 18, 2020 Category: Parasitology Source Type: research

Bar-seq strategies for the LeishGEdit toolbox
Publication date: Available online 10 July 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Tom Beneke, Eva Gluenz (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - July 12, 2020 Category: Parasitology Source Type: research

Nematode endosymbiont competition: Fortune favors the fittest
Publication date: Available online 1 July 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Christa Heryanto, Ioannis Eleftherianos (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - July 3, 2020 Category: Parasitology Source Type: research

Senna plant generates reactive oxygen species (ROS) and induces apoptosis in Hymenolepis diminuta
Publication date: Available online 30 June 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Saptarshi Roy, Nikhilesh Joardar, Santi P. Sinha Babu, Larisha M. Lyndem (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - July 1, 2020 Category: Parasitology Source Type: research

Schistosoma mansoni infection affects the proteome and lipidome of circulating extracellular vesicles in the host
Publication date: Available online 27 June 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Michiel L. Bexkens, Renske A. van Gestel, Bas van Breukelen, Rolf T. Urbanus, Jos F. Brouwers, Rienk Nieuwland, Aloysius G.M. Tielens, Jaap J. van Hellemond (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - June 28, 2020 Category: Parasitology Source Type: research

Analysis of codon usage pattern of mitochondrial ND genes in Platyhelminthes
Publication date: Available online 24 June 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Gulshana A. Mazumder, Arif Uddin, Supriyo Chakraborty (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - June 25, 2020 Category: Parasitology Source Type: research

Effect of Posaconazole in an in vitro model of cardiac fibrosis induced by Trypanosoma cruzi
Publication date: Available online 18 June 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Lindice Mitie Nisimura, Patrícia Mello Ferrão, Alanderson da Rocha Nogueira, Mariana Caldas Waghabi, Marcelo Meuser-Batista, Otacílio C. Moreira, Julio A. Urbina, Luciana Ribeiro Garzoni (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - June 20, 2020 Category: Parasitology Source Type: research

The Entamoeba lysine and glutamic acid rich protein (KERP1) virulence factor gene is present in the genomes of Entamoeba nuttalli, Entamoeba dispar and Entamoeba moshkovskii
Publication date: Available online 11 June 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Gareth D. Weedall (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - June 12, 2020 Category: Parasitology Source Type: research

Use of split-dihydrofolate reductase for the detection of protein-protein interactions and simultaneous selection of multiple plasmids in Plasmodium falciparum
Publication date: Available online 4 June 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Yvette S. Levray, Anne D. Berhe, Andrew R. Osborne (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - June 6, 2020 Category: Parasitology Source Type: research

Structural and biochemical analysis of phosphoethanolamine methyltransferase from the pine wilt nematode Bursaphelenchus xylophilus
Publication date: Available online 30 May 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Soon Goo Lee, Michelle S. Chung, Antea J. DeMarsilis, Cynthia K. Holland, Rohit V. Jaswaney, Cherry Jiang, Jakob H.P. Kroboth, Kevin Kulshrestha, Raymundo Z.W. Marcelo, Vidhya M. Meyyappa, Grant B. Nelson, Janki K. Patel, Alex J. Petronio, Samantha K. Powers, Peter R. Qin, Mythili Ramachandran, Divya Rayapati, John A. Rincon, Andreia Rocha, Joan Gabriel Rodinho Nunes Ferreira (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - May 31, 2020 Category: Parasitology Source Type: research

Comparative study of transcription profiles of the P-glycoprotein transporters of two Haemonchus contortus isolates: susceptible and resistant to ivermectin
Publication date: Available online 17 May 2020Source: Molecular and Biochemical ParasitologyAuthor(s): David E. Reyes-Guerrero, Marilyn Cedillo-Borda, Rogelio Alonso-Morales, Miguel Alonso-Díaz, Agustín Olmedo-Juárez, Pedro Mendoza-de-Gives, Ma. Eugenia López-Arellano (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - May 18, 2020 Category: Parasitology Source Type: research

Editorial Board
Publication date: May 2020Source: Molecular and Biochemical Parasitology, Volume 237Author(s): (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - May 17, 2020 Category: Parasitology Source Type: research

Actin sequestering protein, profilin, regulates intracellular vesicle transport in Leishmania
Publication date: Available online 12 May 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Bindu Ambaru, Anupriya Gopalsamy, T.V.Satish Tammana, Hosahalli S. Subramanya, Chhitar M. Gupta (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - May 13, 2020 Category: Parasitology Source Type: research

Orientation of FtsH protease homologs in Trypanosoma brucei inner mitochondrial membrane and its evolutionary implications
Publication date: Available online 11 May 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Tomáš Kovalinka, Tomáš Pánek, Bianka Kováčová, Anton Horváth (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - May 11, 2020 Category: Parasitology Source Type: research

Vancomycin improves Plasmodium yoelii malaria parasite in vitro liver stage cultures by controlling Elizabethkingia anophelis, a bacterium in the microbiome of lab-reared Anopheles mosquitoes
Publication date: Available online 30 April 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Melanie J. Shears, Sean C. Murphy (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - May 1, 2020 Category: Parasitology Source Type: research

Cryptosporidium parvum cyclic GMP-dependent protein kinase (PKG): An essential mediator of merozoite egress
Publication date: Available online 26 April 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Samantha Nava, Aygul Sadigova, Alejandro Castellanos-Gonzalez, A. Clinton White (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - April 28, 2020 Category: Parasitology Source Type: research

The mitochondrial phosphate carrier TbMCP11 is essential for mitochondrial function in the procyclic form of Trypanosoma brucei
Publication date: Available online 27 April 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Fei Gao, Frank Voncken, Claudia Colasante (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - April 28, 2020 Category: Parasitology Source Type: research

The complexity and diversity of the actin cytoskeleton of trypanosomatids
Publication date: Available online 28 April 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Andrea Vizcaíno-Castillo, Juan Felipe Osorio-Méndez, Javier R. Ambrosio, Roberto Hernández, Ana María Cevallos (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - April 28, 2020 Category: Parasitology Source Type: research

Isolation and characterization of a novel member of the ACC ligand-gated chloride channel family, Hco-LCG-46, from the parasitic nematode Haemonchus contortus
Publication date: Available online 6 April 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Sarah A. Habibi, Stephen M. Blazie, Yishi Jin, Sean G. Forrester (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - April 7, 2020 Category: Parasitology Source Type: research

Characterization of the complete mitogenome of Centrorhynchus clitorideus (Meyer, 1931) (Palaeacanthocephala: Centrorhynchidae), the largest mitochondrial genome in Acanthocephala, and its phylogenetic implications
Publication date: Available online 31 March 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Nehaz Muhammad, Suleman, Mian Sayed Khan, Liang Li, Qing Zhao, Hanif Ullah, Xing-Quan Zhu, Jun Ma (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - April 1, 2020 Category: Parasitology Source Type: research

Editorial Board
Publication date: March 2020Source: Molecular and Biochemical Parasitology, Volume 236Author(s): (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - March 7, 2020 Category: Parasitology Source Type: research

Structural and kinetic characterization of Trypanosoma congolense pyruvate kinase
Publication date: March 2020Source: Molecular and Biochemical Parasitology, Volume 236Author(s): Joar Esteban Pinto Torres, Meng Yuan, Julie Goossens, Wim Versées, Guy Caljon, Paul A. Michels, Malcolm D. Walkinshaw, Stefan Magez, Yann G.-J. Sterckx (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - February 27, 2020 Category: Parasitology Source Type: research

Structural and kinetic characterisation of Trypanosoma congolense pyruvate kinase
Publication date: Available online 19 February 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Joar Esteban Pinto Torres, Meng Yuan, Julie Goossens, Wim Versées, Guy Caljon, Paul A. Michels, Malcolm D. Walkinshaw, Stefan Magez, Yann G.-J. SterckxAbstractTrypanosoma are blood-borne parasites and are the causative agents of neglected tropical diseases (NTDs) affecting both humans and animals. These parasites mainly rely on glycolysis for their energy production within the mammalian host, which is why trypanosomal glycolytic enzymes have been pursued as interesting targets for the development of trypanocid...
Source: Molecular and Biochemical Parasitology - February 19, 2020 Category: Parasitology Source Type: research

YihA GTPases localize to the apicoplast and mitochondrion of the malaria parasite and interact with LSU of organellar ribosomes
Publication date: Available online 11 February 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Ankit Gupta, Kirti Gupta, Saman HabibAbstractThe YihA TRAFAC GTPases are critical for late-stage assembly of the ribosomal large subunit (LSU). In order to explore biogenesis of the reduced organellar ribosomes of the malaria parasite, we identified three nuclear-encoded homologs of YihA in Plasmodium falciparum. PfYihA1 targeted to the parasite apicoplast, PfYihA2 to the mitochondrion, and PfYihA3 was found in both the apicoplast and cytosol. The three PfYihA, expressed as recombinant proteins, were active GTPases a...
Source: Molecular and Biochemical Parasitology - February 13, 2020 Category: Parasitology Source Type: research

The recognition of proteasomal receptors by Plasmodium falciparum DSK2
In this study, we have demonstrated how PfDsk2 and its recognition by proteasomal receptors differ from the mammalian system. Our crystal structure of unbound PfDsk2 UBL domain at 1.30 Å revealed an additional 310-helix compared to the human homolog, as well as a few significant differences in its putative binding interface with the proteasome receptors, PfRpn10 and PfRpn13. Moreover, the non-binding face of UBL showed a reversal of surface charge compared to HsDsk2 shuttle protein, instead resembling HOIL-like E3 ligase UBL domain. The affinity of the interaction with the proteasomal receptors remained simila...
Source: Molecular and Biochemical Parasitology - February 13, 2020 Category: Parasitology Source Type: research

Molecular Basis for Hycanthone Drug Action in Schistosome Parasites
Publication date: Available online 3 February 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Meghan Guzman, Anastasia Rugel, Reid S. Tarpley, Xiaohang Cao, Stanton F. McHardy, Philip T. LoVerde, Alexander B. TaylorAbstractHycanthone (HYC) is a retired drug formerly used to treat schistosomiasis caused by infection from Schistosoma mansoni and S. haematobium. Resistance to HYC was first observed in S. mansoni laboratory strains and in patients in the 1970s and the use of this drug was subsequently discontinued with the substitution of praziquantel (PZQ) as the single antischistosomal drug in the worldwide form...
Source: Molecular and Biochemical Parasitology - February 4, 2020 Category: Parasitology Source Type: research

Corrigendum to “Shotgun proteomics of Strongyloides venezuelensis infective third stage larvae: Insights into host-parasite interaction and novel targets for diagnostic” [Mol. Biochem. Parasitol. 235 (2020) 111249]
Publication date: Available online 3 February 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Priscilla D.M. Fonseca, Marcelo A. Corral, Miguel Cosenza-Contreras, Dirce M.C.L. Meisel, Gessica B. Melo, Milena M.S. Antunes, Maria C.E. Santo, Ronaldo C.B. Gryschek, Julia M. Costa-Cruz, William Castro-Borges, Fabiana M. Paula (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - February 4, 2020 Category: Parasitology Source Type: research

Proteomic analysis of two populations of Schistosoma mansoni-derived extracellular vesicles: 15k pellet and 120k pellet vesicles
Publication date: Available online 31 January 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Desalegn Woldeyohannes Kifle, Mark S. Pearson, Luke Becker, Darren Pickering, Alex Loukas, Javier SotilloAbstractHelminth parasites secrete extracellular vesicles (EVs) into their environment that have potential roles in host-parasite communication, and thus represent potentially useful targets for novel control strategies. Here, we carried out a comprehensive proteomic analysis of two different populations of EVs – 15k pellet and 120k pellet EVs – from Schistosoma mansoni adult worms. We characterised the...
Source: Molecular and Biochemical Parasitology - February 1, 2020 Category: Parasitology Source Type: research

Observation of morphological changes of female osmiophilic bodies prior to Plasmodium gametocyte egress from erythrocytes
In this study, we investigate the dynamics of OBs in activated gametocytes of Plasmodium falciparum and Plasmodium yoelii using the female OB-specific marker protein, G377. After activation, female gametocyte OBs migrate to the parasite surface and fuse to form large vesicles beneath the parasite plasma membrane. At the marginal region of female gametocytes, fused vesicles secrete contents by exocytosis into the parasitophorous vacuole space, prior to parasite egress via the break-down of the erythrocyte membrane. This is the first detailed description of how proteins are transported through osmiophilic bodies. (Source: Mo...
Source: Molecular and Biochemical Parasitology - January 23, 2020 Category: Parasitology Source Type: research

Translation initiation factors GleIF4E2 and GleIF4A can interact directly with the components of the pre-initiation complex to facilitate translation initiation in Giardia lamblia
Publication date: Available online 20 January 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Adebanjo Najeem Adedoja, Timothy McMahan, John Patrick Neal, Siddhartha Hamal Dhakal, Seetharama Jois, Daniel Romo, Ken Hull, Srinivas GarlapatiAbstractTranslation initiation factor eIF4F is essential for cap-dependent translation initiation in eukaryotes. eIF4F is a trimeric complex consisting of a scaffold protein eIF4G, cap-binding protein eIF4E and DEAD-box RNA helicase eIF4A. eIF4F binds to the 5’ cap structure of the mRNA through eIF4E and facilitates the binding of the preinitiation complex (PIC) via prot...
Source: Molecular and Biochemical Parasitology - January 20, 2020 Category: Parasitology Source Type: research

Functional analysis of vasa/PL10-like genes in the ovary of Schistosoma mansoni
Publication date: Available online 17 January 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Danielle E. Skinner, Anastas Popratiloff, Yousef N. Alrefaei, Victoria H. Mann, Gabriel Rinaldi, Paul J. BrindleyAbstractThe RNA helicase Vasa plays a pivotal role in the development of the germ line. To decipher the functional roles of vasa/PL10-like genes in the human blood fluke Schistosoma mansoni, we performed RNA interference followed by the analysis of the ovary in the adult female. Doubled stranded RNA targeting the schistosome vasa-like gene Smvlg1 reduced the volume of the ovary. Changes in morphology of the...
Source: Molecular and Biochemical Parasitology - January 17, 2020 Category: Parasitology Source Type: research

Molecular Detection of Trichostrongylus Species through PCR Followed by High Resolution Melt Analysis of ITS-2 rDNA Sequences
The objective of the present study was, for the first time, to develop a PCR-HRM assay for the detection of various animal Trichostrongylus spp. A pair of primers targeting the ITS-2 rDNA region of the Trichostrongylus spp. was designed for the development of the HRM assay. DNA samples were extracted from 30 adult worms of Trichostrongylus spp., the ITS-2-rDNA region was amplified using PCR, and the resultant products were sequenced and characterized. Afterwards, the PCR-HRM analysis was conducted to detect and discriminate Trichostrongylus spp. Molecular sequence analysis revealed that 24, 4, and 1 of the samples were T. ...
Source: Molecular and Biochemical Parasitology - January 17, 2020 Category: Parasitology Source Type: research

Editorial Board
Publication date: January 2020Source: Molecular and Biochemical Parasitology, Volume 235Author(s): (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - January 10, 2020 Category: Parasitology Source Type: research

Shotgun proteomics of Strongyloides venezuelensis infective third stage larvae: Insights into host–parasite interaction and novel targets for diagnostics
This study presents an overview of the protein iL3 S. venezuelensis focusing on the diagnosis of strongyloidiasis. A total of 877 proteins were identified by shotgun proteomics. Many of these proteins are involved in different cellular processes, metabolic as well as structural maintenance. Our results point to a catalog of possible diagnostic targets for human strongyloidiasis and highlight the need for evaluation of uncharacterized proteins, especially the proteins within the CAP domain, transthyretin, and BTPI inhibitor domains, as a repertoire as yet unexplored in the context of strongyloidiasis diagnostic markers. We ...
Source: Molecular and Biochemical Parasitology - December 25, 2019 Category: Parasitology Source Type: research

Polarization of MTIP is a signature of gliding locomotion in Plasmodium ookinetes and sporozoites
Publication date: Available online 23 December 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Inga Siden-Kiamos, Christian Goosmann, Carlos A. Buscaglia, Volker Brinkmann, Kai Matuschewski, Georgina N. MontagnaAbstractGliding motility and cell invasion are essential for the successful transmission of Plasmodium parasites. These processes rely on an acto-myosin motor located underneath the parasite plasma membrane. The Myosin A-tail interacting protein (MTIP) connects the class XIV myosin A (MyoA) to the gliding-associated proteins and is essential for assembly of the motor at the inner membrane complex. Here,...
Source: Molecular and Biochemical Parasitology - December 25, 2019 Category: Parasitology Source Type: research

Effect of cysteine peptidase inhibitor of Eudiplozoon nipponicum (Monogenea) on cytokine expression of macrophages in vitro
Publication date: Available online 23 December 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Jana Ilgová, Lenka Kavanová, Katarína Matiašková, Jiří Salát, Martin KašnýAbstractThe gills of the common carp, whose mucosal surface belongs to the key defence mechanisms of piscine immunity, can be infested with both the larval and adult stage of Eudiplozoon nipponicum (Monogenea). Although on their own, monogeneans do not considerably compromise their hosts’ health status, fish with epithelial barriers damaged in parasite feeding and attachment ...
Source: Molecular and Biochemical Parasitology - December 25, 2019 Category: Parasitology Source Type: research

Expression and copper binding properties of the N-terminal domain of copper P-type ATPases of African trypanosomes
In conclusion, Trypanosoma species express a copper-transporting P1B-1-type ATPase and together with other copper-binding proteins identified in the genomes of kinetoplastid parasites may constitute potential targets for anti-trypanosomal drug discovery.Graphical abstract (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - November 19, 2019 Category: Parasitology Source Type: research

Trypanosoma brucei: inhibition of cathepsin L is sufficient to kill bloodstream forms
Publication date: Available online 16 November 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Dietmar Steverding, Stuart A. Rushworth, Bogdan I. Florea, Herman S. OverkleeftAbstractThe lysosomal cysteine protease activity of Trypanosoma brucei comprises a cathepsin B enzyme (TbCATB) and a cathepsin L enzyme (TbCATL). Inhibition of the cysteine protease activity is lethal to bloodstream-form trypanosomes but it was not entirely clear which of the two enzymes are essential for survival of the parasites. Here we show that the vinyl sulfone compound LU-102 selectively inhibits TbCATL without affecting TbCATB and ...
Source: Molecular and Biochemical Parasitology - November 18, 2019 Category: Parasitology Source Type: research

Brugia malayi galectin 2 is a tandem-repeat type galectin capable of binding mammalian polysaccharides
Publication date: Available online 15 November 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Marla I Hertz, Philip M Glaessner, Amy Rush, Philip J BudgeAbstractGalectins are among the most abundant excretory/secretory (ES) products of filarial worms, but their role in filarial biology is poorly understood. Galectin-2 (Lec-2), a major component of Brugia malayi extracellular vesicles, is released by filarial worms, and was recently identified in the serum of persons with loiasis. We therefore sought to clone and characterize Lec-2, and to develop reagents to examine its potential as a biomarker and its role i...
Source: Molecular and Biochemical Parasitology - November 16, 2019 Category: Parasitology Source Type: research

Editorial Board
Publication date: December 2019Source: Molecular and Biochemical Parasitology, Volume 234Author(s): (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - November 16, 2019 Category: Parasitology Source Type: research

Genome-wide identification of ABC transporters in monogeneans
Publication date: Available online 9 November 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Víctor Caña-Bozada, F. Neptalí Morales-Serna, Alejandra García-Gasca, Raúl Llera-Herrera, Emma J. Fajer-ÁvilaAbstractATP-Binding Cassette (ABC) transporters are proteins that actively mediate the transport of a wide variety of molecules, including drugs. Thus, in parasitology, ABC transporters have gained attention as potential targets for therapeutic drugs. Among the parasitic Platyhelminthes, ABC transporters have been identified and classified in a few species of Trematoda ...
Source: Molecular and Biochemical Parasitology - November 9, 2019 Category: Parasitology Source Type: research

Synthetic small molecule analogues of the immunomodulatory Acanthocheilonema viteae product ES-62 promote metabolic homeostasis during obesity in a mouse model
Publication date: Available online 18 October 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Felicity E. Lumb, Jenny Crowe, James Doonan, Colin J. Suckling, Colin Selman, Margaret M. Harnett, William HarnettAbstractOne of the most rapidly increasing human public health problems is obesity, whose sequelae like type-2 diabetes, represent continuously worsening, life-long conditions. Over the last 15 years, data have begun to emerge from human and more frequently, mouse studies, that support the idea that parasitic worm infection can protect against this condition. We have therefore investigated the potential of...
Source: Molecular and Biochemical Parasitology - October 19, 2019 Category: Parasitology Source Type: research

Molecular characterization of Schistosoma mansoni tegument annexins and comparative analysis of antibody responses following parasite infection
In conclusion, the overall results present the molecular characterization of annexin B30, annexin B5a, annexin B7a and annexin B5b from S. mansoni in host-parasite interactions and strongly suggest that the molecules could be useful candidates for vaccine or diagnostic development. (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - October 19, 2019 Category: Parasitology Source Type: research

Phosphorylation of eukaryotic initiation factor-2α in response to endoplasmic reticulum and nitrosative stress in the human protozoan parasite, Entamoeba histolytica
We examined the morphology of the ER, tracked phosphorylation of EheIF-2α, and assessed protein translation in control and stressed cells. While all four stress-inducing reagents caused a global reduction in protein translation, only DTT was capable of also inducing changes in the morphology of the ER (consistent with ER stress) and phosphorylation of EheIF-2α. This suggests that DTT authentically induces ER stress in E. histolytica and that this stress is managed by the eIF-2α-based system. This was supported by the observation that cells expressing a non-phosphorylatable version of eIF-2α were als...
Source: Molecular and Biochemical Parasitology - October 10, 2019 Category: Parasitology Source Type: research