Observation of morphological changes of female osmiophilic bodies prior to Plasmodium gametocyte egress from erythrocytes
In this study, we investigate the dynamics of OBs in activated gametocytes of Plasmodium falciparum and Plasmodium yoelii using the female OB-specific marker protein, G377. After activation, female gametocyte OBs migrate to the parasite surface and fuse to form large vesicles beneath the parasite plasma membrane. At the marginal region of female gametocytes, fused vesicles secrete contents by exocytosis into the parasitophorous vacuole space, prior to parasite egress via the break-down of the erythrocyte membrane. This is the first detailed description of how proteins are transported through osmiophilic bodies. (Source: Mo...
Source: Molecular and Biochemical Parasitology - January 23, 2020 Category: Parasitology Source Type: research

Translation initiation factors GleIF4E2 and GleIF4A can interact directly with the components of the pre-initiation complex to facilitate translation initiation in Giardia lamblia
Publication date: Available online 20 January 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Adebanjo Najeem Adedoja, Timothy McMahan, John Patrick Neal, Siddhartha Hamal Dhakal, Seetharama Jois, Daniel Romo, Ken Hull, Srinivas GarlapatiAbstractTranslation initiation factor eIF4F is essential for cap-dependent translation initiation in eukaryotes. eIF4F is a trimeric complex consisting of a scaffold protein eIF4G, cap-binding protein eIF4E and DEAD-box RNA helicase eIF4A. eIF4F binds to the 5’ cap structure of the mRNA through eIF4E and facilitates the binding of the preinitiation complex (PIC) via prot...
Source: Molecular and Biochemical Parasitology - January 20, 2020 Category: Parasitology Source Type: research

Functional analysis of vasa/PL10-like genes in the ovary of Schistosoma mansoni
Publication date: Available online 17 January 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Danielle E. Skinner, Anastas Popratiloff, Yousef N. Alrefaei, Victoria H. Mann, Gabriel Rinaldi, Paul J. BrindleyAbstractThe RNA helicase Vasa plays a pivotal role in the development of the germ line. To decipher the functional roles of vasa/PL10-like genes in the human blood fluke Schistosoma mansoni, we performed RNA interference followed by the analysis of the ovary in the adult female. Doubled stranded RNA targeting the schistosome vasa-like gene Smvlg1 reduced the volume of the ovary. Changes in morphology of the...
Source: Molecular and Biochemical Parasitology - January 17, 2020 Category: Parasitology Source Type: research

Molecular Detection of Trichostrongylus Species through PCR Followed by High Resolution Melt Analysis of ITS-2 rDNA Sequences
The objective of the present study was, for the first time, to develop a PCR-HRM assay for the detection of various animal Trichostrongylus spp. A pair of primers targeting the ITS-2 rDNA region of the Trichostrongylus spp. was designed for the development of the HRM assay. DNA samples were extracted from 30 adult worms of Trichostrongylus spp., the ITS-2-rDNA region was amplified using PCR, and the resultant products were sequenced and characterized. Afterwards, the PCR-HRM analysis was conducted to detect and discriminate Trichostrongylus spp. Molecular sequence analysis revealed that 24, 4, and 1 of the samples were T. ...
Source: Molecular and Biochemical Parasitology - January 17, 2020 Category: Parasitology Source Type: research

Editorial Board
Publication date: January 2020Source: Molecular and Biochemical Parasitology, Volume 235Author(s): (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - January 10, 2020 Category: Parasitology Source Type: research

Shotgun proteomics of Strongyloides venezuelensis infective third stage larvae: Insights into host–parasite interaction and novel targets for diagnostics
This study presents an overview of the protein iL3 S. venezuelensis focusing on the diagnosis of strongyloidiasis. A total of 877 proteins were identified by shotgun proteomics. Many of these proteins are involved in different cellular processes, metabolic as well as structural maintenance. Our results point to a catalog of possible diagnostic targets for human strongyloidiasis and highlight the need for evaluation of uncharacterized proteins, especially the proteins within the CAP domain, transthyretin, and BTPI inhibitor domains, as a repertoire as yet unexplored in the context of strongyloidiasis diagnostic markers. We ...
Source: Molecular and Biochemical Parasitology - December 25, 2019 Category: Parasitology Source Type: research

Polarization of MTIP is a signature of gliding locomotion in Plasmodium ookinetes and sporozoites
Publication date: Available online 23 December 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Inga Siden-Kiamos, Christian Goosmann, Carlos A. Buscaglia, Volker Brinkmann, Kai Matuschewski, Georgina N. MontagnaAbstractGliding motility and cell invasion are essential for the successful transmission of Plasmodium parasites. These processes rely on an acto-myosin motor located underneath the parasite plasma membrane. The Myosin A-tail interacting protein (MTIP) connects the class XIV myosin A (MyoA) to the gliding-associated proteins and is essential for assembly of the motor at the inner membrane complex. Here,...
Source: Molecular and Biochemical Parasitology - December 25, 2019 Category: Parasitology Source Type: research

Effect of cysteine peptidase inhibitor of Eudiplozoon nipponicum (Monogenea) on cytokine expression of macrophages in vitro
Publication date: Available online 23 December 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Jana Ilgová, Lenka Kavanová, Katarína Matiašková, Jiří Salát, Martin KašnýAbstractThe gills of the common carp, whose mucosal surface belongs to the key defence mechanisms of piscine immunity, can be infested with both the larval and adult stage of Eudiplozoon nipponicum (Monogenea). Although on their own, monogeneans do not considerably compromise their hosts’ health status, fish with epithelial barriers damaged in parasite feeding and attachment ...
Source: Molecular and Biochemical Parasitology - December 25, 2019 Category: Parasitology Source Type: research

Expression and copper binding properties of the N-terminal domain of copper P-type ATPases of African trypanosomes
In conclusion, Trypanosoma species express a copper-transporting P1B-1-type ATPase and together with other copper-binding proteins identified in the genomes of kinetoplastid parasites may constitute potential targets for anti-trypanosomal drug discovery.Graphical abstract (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - November 19, 2019 Category: Parasitology Source Type: research

Trypanosoma brucei: inhibition of cathepsin L is sufficient to kill bloodstream forms
Publication date: Available online 16 November 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Dietmar Steverding, Stuart A. Rushworth, Bogdan I. Florea, Herman S. OverkleeftAbstractThe lysosomal cysteine protease activity of Trypanosoma brucei comprises a cathepsin B enzyme (TbCATB) and a cathepsin L enzyme (TbCATL). Inhibition of the cysteine protease activity is lethal to bloodstream-form trypanosomes but it was not entirely clear which of the two enzymes are essential for survival of the parasites. Here we show that the vinyl sulfone compound LU-102 selectively inhibits TbCATL without affecting TbCATB and ...
Source: Molecular and Biochemical Parasitology - November 18, 2019 Category: Parasitology Source Type: research

Brugia malayi galectin 2 is a tandem-repeat type galectin capable of binding mammalian polysaccharides
Publication date: Available online 15 November 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Marla I Hertz, Philip M Glaessner, Amy Rush, Philip J BudgeAbstractGalectins are among the most abundant excretory/secretory (ES) products of filarial worms, but their role in filarial biology is poorly understood. Galectin-2 (Lec-2), a major component of Brugia malayi extracellular vesicles, is released by filarial worms, and was recently identified in the serum of persons with loiasis. We therefore sought to clone and characterize Lec-2, and to develop reagents to examine its potential as a biomarker and its role i...
Source: Molecular and Biochemical Parasitology - November 16, 2019 Category: Parasitology Source Type: research

Editorial Board
Publication date: December 2019Source: Molecular and Biochemical Parasitology, Volume 234Author(s): (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - November 16, 2019 Category: Parasitology Source Type: research

Genome-wide identification of ABC transporters in monogeneans
Publication date: Available online 9 November 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Víctor Caña-Bozada, F. Neptalí Morales-Serna, Alejandra García-Gasca, Raúl Llera-Herrera, Emma J. Fajer-ÁvilaAbstractATP-Binding Cassette (ABC) transporters are proteins that actively mediate the transport of a wide variety of molecules, including drugs. Thus, in parasitology, ABC transporters have gained attention as potential targets for therapeutic drugs. Among the parasitic Platyhelminthes, ABC transporters have been identified and classified in a few species of Trematoda ...
Source: Molecular and Biochemical Parasitology - November 9, 2019 Category: Parasitology Source Type: research

Synthetic small molecule analogues of the immunomodulatory Acanthocheilonema viteae product ES-62 promote metabolic homeostasis during obesity in a mouse model
Publication date: Available online 18 October 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Felicity E. Lumb, Jenny Crowe, James Doonan, Colin J. Suckling, Colin Selman, Margaret M. Harnett, William HarnettAbstractOne of the most rapidly increasing human public health problems is obesity, whose sequelae like type-2 diabetes, represent continuously worsening, life-long conditions. Over the last 15 years, data have begun to emerge from human and more frequently, mouse studies, that support the idea that parasitic worm infection can protect against this condition. We have therefore investigated the potential of...
Source: Molecular and Biochemical Parasitology - October 19, 2019 Category: Parasitology Source Type: research

Molecular characterization of Schistosoma mansoni tegument annexins and comparative analysis of antibody responses following parasite infection
In conclusion, the overall results present the molecular characterization of annexin B30, annexin B5a, annexin B7a and annexin B5b from S. mansoni in host-parasite interactions and strongly suggest that the molecules could be useful candidates for vaccine or diagnostic development. (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - October 19, 2019 Category: Parasitology Source Type: research

Phosphorylation of eukaryotic initiation factor-2α in response to endoplasmic reticulum and nitrosative stress in the human protozoan parasite, Entamoeba histolytica
We examined the morphology of the ER, tracked phosphorylation of EheIF-2α, and assessed protein translation in control and stressed cells. While all four stress-inducing reagents caused a global reduction in protein translation, only DTT was capable of also inducing changes in the morphology of the ER (consistent with ER stress) and phosphorylation of EheIF-2α. This suggests that DTT authentically induces ER stress in E. histolytica and that this stress is managed by the eIF-2α-based system. This was supported by the observation that cells expressing a non-phosphorylatable version of eIF-2α were als...
Source: Molecular and Biochemical Parasitology - October 10, 2019 Category: Parasitology Source Type: research

Editorial Board
Publication date: October 2019Source: Molecular and Biochemical Parasitology, Volume 233Author(s): (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - October 4, 2019 Category: Parasitology Source Type: research

Nucleotide sugar transporters of Entamoeba histolytica and Entamoeba invadens involved in chitin synthesis
In this study, we have identified the putative UDP-GlcNAc transporter (EiNst5) of Ei by BLASTP analysis using the amino acid sequence of EhNst3, the UDP-GlcNAc transporter of Eh. Heterologous expression of both EhNst3 and EiNst5 was found to complement the function of Yea4p (UDP-GlcNAc transporter of S. cerevisiae) in YEA4 null mutant and increased the cell wall chitin content. Like Yea4p in S. cerevisiae, Myc-epitope tagged EhNst3 and EiNst5 were localized to the endoplasmic reticulum in Δyea4 cells. The EiNST5 transcript was up-regulated during the in vitro encystation and oxidative stress in E. invad...
Source: Molecular and Biochemical Parasitology - October 3, 2019 Category: Parasitology Source Type: research

Phosphorylation of Eukaryotic Initiation Factor 2-α in Response to Endoplasmic Reticulum and Nitrosative Stress in the human protozoan parasite, Entamoeba histolytica
We examined the morphology of the ER, tracked phosphorylation of EheIF2α, and assessed protein translation in control and stressed cells. While all four stress-inducing reagents caused a global reduction in protein translation, only DTT was capable of also inducing changes in the morphology of the ER (consistent with ER stress) and phosphorylation of EheIF-2α. This suggests that DTT authentically induces ER stress in E. histolytica and that this stress is managed by the eIF2α-based system. This was supported by the observation that cells expressing a non-phosphorylatable version of eIF-2α were also ...
Source: Molecular and Biochemical Parasitology - September 29, 2019 Category: Parasitology Source Type: research

A membrane-associated metalloprotease of Schistosoma japonicum structurally related to the FACE-1/Ste24p protease family
In this study, a full-length cDNA putatively encoding a FACE-1/Ste24p CaaX protease (type I) of the Schistosoma japonicum was isolated. The cDNA, named SjSte24p, composed of 1,646 bp and encoded 473 amino acids with predicted Mr/pI as 54.77 kDa/8.04. SjSte24p is a monoexonic gene constantly expressed in the parasite from cercariae to adult stages. It contained the characteristic of CaaX protease topology, including seven trans-membrane domains and a metallo-protease segment with a zinc-binding motif (HEXXH). SjSte24p shared a considerable degree of sequence identity with the type I CaaX proteases. A phylogenetic ana...
Source: Molecular and Biochemical Parasitology - September 21, 2019 Category: Parasitology Source Type: research

Characterisation of a mitochondrial iron transporter of the pathogen Trypanosoma brucei
Publication date: Available online 19 September 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Fuli Zheng, Claudia Colasante, Frank VonckenAbstractSimilar to higher eukaryotes, the protist parasite T. brucei harbours several iron-containing proteins that regulate DNA and protein processing, oxidative stress defence and mitochondrial respiration. The synthesis of these proteins occurs either in the cytoplasm or within the mitochondrion. For mitochondrial iron cluster protein synthesis, iron needs to be transported across the solute impermeable mitochondrial membrane. In T. brucei we previously identified 24 mi...
Source: Molecular and Biochemical Parasitology - September 21, 2019 Category: Parasitology Source Type: research

Helminth-microbiota cross-talk – a journey through the vertebrate digestive system
Publication date: Available online 18 September 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Alba Cortés, Laura Peachey, Riccardo Scotti, Timothy P Jenkins, Cinzia CantacessiAbstractThe gastrointestinal (GI) tract of vertebrates is inhabited by a vast array of organisms, that is, the microbiota and macrobiota. The former is composed largely of commensal microorganisms, which play vital roles in host nutrition and maintenance of energy balance, in addition to supporting the development and function of the vertebrate immune system. By contrast, the macrobiota includes parasitic helminths, which are mos...
Source: Molecular and Biochemical Parasitology - September 20, 2019 Category: Parasitology Source Type: research

Excystation of Eimeria acervulina, E. maxima, and E. tenella differs in response to trypsin and chymotrypsin and the presence of reducing agents DTT and TCEP
In this study, it was found that adding a reducing agent, either dithiothreitol (DTT) or Tris(2-carboxyethyl)phosphine hydrochloride (TCEP), increased the lifespan of sporozoites released from Eimeria maxima. While the addition of DTT or TCEP affected the apparent molecular weight of trypsin, it did not interfere with excystation of E. maxima, but rather had a positive effect on the number of viable sporozoites present after release. This effect was time-dependent in that the number of intact sporozoites at 15 and 30 min after excystation was similar between untreated and DTT- or TCEP-treated sporocysts. However, by...
Source: Molecular and Biochemical Parasitology - September 12, 2019 Category: Parasitology Source Type: research

Nuclear Hormone Receptors in Parasitic Platyhelminths
Publication date: Available online 27 August 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Wenjie Wu, Philip T. LoVerdeAbstractNuclear receptors (NRs) belong to a large protein superfamily which includes intracellular receptors for secreted hydrophobic signal molecules, such as steroid hormones and thyroid hormones. They regulate development and reproduction in metazoans by binding to the promoter region of their target gene to activate or repress mRNA synthesis. Isolation and characterization of NRs in the parasitic trematode Schistosoma mansoni identified two homologues of mammalian thyroid receptor (TR). ...
Source: Molecular and Biochemical Parasitology - August 28, 2019 Category: Parasitology Source Type: research

Editorial Board
Publication date: September 2019Source: Molecular and Biochemical Parasitology, Volume 232Author(s): (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - August 28, 2019 Category: Parasitology Source Type: research

Isolation and partial structural characterization of new Kunitz-type trypsin inhibitors from the pike cestode Triaenophorus nodulosus
Publication date: Available online 24 August 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Eugene A. Rogozhin, Mikhail M. Solovyev, Tatiana V. Frolova, Galina I. IzvekovaAbstractThe inhibitors produced by the parasitic worms successfully protect them from the host’s proteases and are supposed to underlie the host-parasite specificity. Our previous study has shown that the extracts from the pike tapeworm Triaenophorus nodulosus inhibit host proteinases and commercial trypsin. We aimed to isolate and identify the components responsible for trypsin inactivation. After a two-step separation the molecular m...
Source: Molecular and Biochemical Parasitology - August 25, 2019 Category: Parasitology Source Type: research

Leishmania amazonensis inorganic phosphate transporter system is increased in the proliferative forms
Publication date: Available online 21 August 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Luiz Fernando Carvalho-Kelly, André Luiz Gomes-Vieira, Lisvane Paes-Vieira, Amanda Durval Zeferino da Silva, José Roberto Meyer-FernandesAbstractHere we characterize a high-affinity Pi transport system energized by a H+ gradient in Leishmania amazonensis. Pi uptake and transcription of LamPho84 gene are differentially regulated during parasite life cycle. Our data suggest that Pi acquisition could be a pivotal task for the success of the parasite throughout its life cycle.Graphical abstract (Source: Molec...
Source: Molecular and Biochemical Parasitology - August 22, 2019 Category: Parasitology Source Type: research

A novel GCN5b lysine acetyltransferase complex associates with distinct transcription factors in the protozoan parasite Toxoplasma gondii
We report the greatest resolution of the GCN5b complex to date under these various culture conditions. Moreover, reciprocal co-IPs were performed with distinct GCN5b-interacting AP2 factors (AP2IX-7 and AP2XII-4) to delineate the interactomes of each putative transcription factor. Our findings suggest that GCN5b is associated with at least two distinct complexes that are characterized by two different pairs of AP2 factors, and implicate up to four AP2 proteins to be involved with GCN5b-mediated gene regulation.Graphical abstract (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - August 3, 2019 Category: Parasitology Source Type: research

Same same, but different: uncovering unique features of the mitochondrial respiratory chain of apicomplexans
Publication date: Available online 2 August 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Jenni A. Hayward, Giel G. van DoorenAbstractMitochondrial respiration is a critical process for the survival of many eukaryotes, including parasites in the phylum Apicomplexa. These intracellular parasites include the causative agents of numerous serious diseases in humans and animals, including toxoplasmosis (Toxoplasma gondii) and malaria (Plasmodium species). Emerging evidence indicates that the mitochondrial respiratory chain of apicomplexans has notable differences to that of the host cells they infect. These diffe...
Source: Molecular and Biochemical Parasitology - August 3, 2019 Category: Parasitology Source Type: research

Chorismate synthase from malaria parasites is bifunctional enzyme
Publication date: Available online 2 August 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Harvinder Kour Khera, Susheel Kumar Singh, Subhash SinghAbstractChorismate synthase (Cs) is the last enzyme of the main trunk of shikimate pathway and catalyzes formation of chorismate, a major aromatic metabolite precursor. We have previously reported that Cs is highly conserved across different Plasmodium sp. Here we report that Cs from malaria parasites are bifunctional enzymes through expression and functional studies of two recombinant proteins rPfCs (Cs from P. falciparum) and rPvCs (Cs from P. vivax). We confirm ...
Source: Molecular and Biochemical Parasitology - August 3, 2019 Category: Parasitology Source Type: research

Profiling circulating microRNAs in serum of Fasciola gigantica-infected buffalo
In this study, a total of 121 host circulating miRNAs were differentially expressed (2 ≥ fold change, p 
Source: Molecular and Biochemical Parasitology - August 2, 2019 Category: Parasitology Source Type: research

Hookworm infection aggravates metabolic disorder in obesity
Publication date: Available online 12 July 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Vivian Jordania da Silva, Sílvia Regina Costa Dias, William Pereira Alves, Luis Fernando Viana Furtado, Luciana Ribeiro Serafim, Thayse Batista Moreira, Pedro Henrique Nascimento de Aguiar, Ana Maria Caetano de Faria, Élida Mara Leite RabeloAbstractObesity and ancylostomiasis are considered public health problems. Recent studies have shown that infection by intestinal helminths in obese individuals can ameliorate metabolic disorder and improve glucose tolerance by decreasing both insulin resistance and low-...
Source: Molecular and Biochemical Parasitology - July 14, 2019 Category: Parasitology Source Type: research

An enigmatic catalase of Blastocrithidia
Publication date: Available online 2 July 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Claretta Bianchi, Alexei Yu. Kostygov, Natalya Kraeva, Kristína Záhonová, Eva Horáková, Roman Sobotka, Julius Lukeš, Vyacheslav YurchenkoAbstractHere we report that trypanosomatid flagellates of the genus Blastocrithidia possess catalase. This enzyme is not phylogenetically related to the previously characterized catalases in other monoxenous trypanosomatids, suggesting that their genes have been acquired independently. Surprisingly, Blastocrithidia catalase is less enzymatically ...
Source: Molecular and Biochemical Parasitology - July 3, 2019 Category: Parasitology Source Type: research

Heavy and light chain homologs of ferritin are essential for blood-feeding and egg production of the ectoparasitic copepod Lepeophtheirus salmonis
Publication date: Available online 25 June 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Erna Irene Heggland, Christiane Tröße, Christiane Eichner, Frank NilsenAbstract•The salmon louse, Lepeophtheirus salmonis, is a hematophagous ectoparasite of salmonid fish. Due to its blood-feeding activity, the louse is exposed to great amounts of iron, which is an essential, yet potentially toxic mineral. The major known iron storage protein is ferritin, which the salmon louse encodes four genes of (LsFer1-4). Two of the ferritins are predicted to be secreted. These are one of the heavy chain homologs (...
Source: Molecular and Biochemical Parasitology - June 27, 2019 Category: Parasitology Source Type: research

Plasmodium berghei sporozoite specific genes- PbS10 and PbS23/SSP3 are required for the development of Exo-erythrocytic Forms
Publication date: Available online 26 June 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Jyothi Togiri, Rameswara Reddy Segireddy, Babu S. Mastan, Dipti Singh, Surendra Kumar Kolli, Ankit Ghosh, Faisal Mohammed Abdul Al-Nihmi, Mulaka Maruthi, Hadi Hasan Choudhary, Sandeep Dey, Satish Mishra, Kota Arun KumarAbstractPlasmodium sporozoites are infective forms of the parasite to mammalian hepatocytes. Sporozoite surface or secreted proteins likely play an important role in recognition, invasion and successful establishment of hepatocyte infection. By approaches of reverse genetics, we report the functional analy...
Source: Molecular and Biochemical Parasitology - June 27, 2019 Category: Parasitology Source Type: research

Editorial Board
Publication date: July 2019Source: Molecular and Biochemical Parasitology, Volume 231Author(s): (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - June 15, 2019 Category: Parasitology Source Type: research

Schistosomes can hydrolyze proinflammatory and prothrombotic polyphosphate (polyP) via tegumental alkaline phosphatase, SmAP
Publication date: Available online 30 May 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Manal Elzoheiry, Akram A. Da’dara, Catherine S. Nation, Samar N. El-Beshbishi, Patrick J. SkellyAbstractSchistosoma mansoni is a long-lived intravascular trematode parasite that can infect humans causing the chronic debilitating disease, schistosomiasis. We hypothesize that the action of host-interactive proteins found at the schistosome surface allows the worms to maintain a safe, anti-thrombotic and anti-inflammatory environment around them in the bloodstream. One such protein is the ˜60 kDa alkaline ...
Source: Molecular and Biochemical Parasitology - May 31, 2019 Category: Parasitology Source Type: research

The Plasmodium falciparum MESA erythrocyte cytoskeleton-binding (MEC) motif binds to erythrocyte ankyrin
In conclusion, we have provided multiple lines of evidence that the MEC motif binds to erythrocyte ANK1. (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - May 23, 2019 Category: Parasitology Source Type: research

Yield improvement and enzymatic dissection of Plasmodium falciparum plasmepsin V
In this study, we employed a multipurpose fusion tag to improve the production of PfPMV in E. coli. Recombinant PfPMVm, comprising residues 84 to 521, was substantially obtained in soluble form and could be purified in a single step, yielding a 3.7-fold increase in purified PfPMVm compared to previous reports. Additionally, we have mutated the catalytic residues (D118 N and D365 N), individually and together, and the unpaired cysteine residue C178 to evaluate the effects on catalytic efficiency. Mutation of D365 had more pronounced effects on the catalytic efficiency than that of D118, suggesting that the D36...
Source: Molecular and Biochemical Parasitology - May 18, 2019 Category: Parasitology Source Type: research

Characterization of a Schistosoma mansoni NDPK expressed in sexual and digestive organs
Publication date: Available online 16 May 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Juliana Roberta Torini, Adriano de Freitas Fernandes, Vitor Hugo Balasco Serrão, Larissa Romanello, Louise E Bird, Joanne E Nettleship, Raymond J. Owens, José Brandão-Neto, Ana Eliza Zeraik, Ricardo DeMarco, Humberto D'Muniz PereiraAbstractNucleoside diphosphate kinases (NDPKs) are crucial to keep the high triphosphate nucleotide levels in the biological process. The enzymatic mechanism has been extensively described; however, the structural characteristics and kinetic parameters have never been fully...
Source: Molecular and Biochemical Parasitology - May 17, 2019 Category: Parasitology Source Type: research

Editorial Board
Publication date: June 2019Source: Molecular and Biochemical Parasitology, Volume 230Author(s): (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - May 15, 2019 Category: Parasitology Source Type: research

Palmitoylation in apicomplexan parasites: from established regulatory roles to putative new functions
Publication date: Available online 9 April 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Maria M. Corvi, Valeria R. TurowskiAbstractThis minireview aims to provide a comprehensive synthesis on protein palmitoylation in apicomplexan parasites and higher eukaryotes where most of the data is available. Apicomplexan parasites encompass numerous obligate intracellular parasites with significant health risk to animals and humans. Protein palmitoylation is a widespread post-translational modification that plays important regulatory roles in several physiological and pathological states. Functional studies demonstra...
Source: Molecular and Biochemical Parasitology - April 10, 2019 Category: Parasitology Source Type: research

Editorial Board
Publication date: April 2019Source: Molecular and Biochemical Parasitology, Volume 229Author(s): (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - March 29, 2019 Category: Parasitology Source Type: research

Farnesyl pyrophosphate synthase is essential for the promastigote and amastigote stages in Leishmania major
Publication date: Available online 26 March 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Sumit Mukherjee, Somrita Basu, Kai ZhangAbstractIsoprenoid synthesis provides a diverse class of biomolecules including sterols, dolichols, ubiquinones and prenyl groups. The enzyme farnesyl pyrophosphate synthase (FPPS) catalyzes the formation of farnesyl pyrophosphate, a key intermediate for the biosynthesis of all isoprenoids. In Leishmania, FPPS is considered the main target of nitrogen containing bisphosphonates, yet the essentiality of this enzyme remains untested. Using a facilitated knockout approach, we carried...
Source: Molecular and Biochemical Parasitology - March 27, 2019 Category: Parasitology Source Type: research

In-solution behavior and protective potential of asparagine synthetase A from Trypanosoma cruzi
Publication date: Available online 15 March 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Flávia Souza Morini, Emanuella de Castro, Stephanie Bath de Morais, Cesar Lancheros Contreras, Ana Valéria Pereira Weiler, Mario Tyago Murakami, Phileno Pinge-Filho, Sueli Fumie Yamada-Ogatta, Tatiana de Arruda Campos Brasil de SouzaAbstractL-Asparagine synthetase (AS) acts in asparagine formation and can be classified into two families: AS-A or AS-B. AS-A is mainly found in prokaryotes and can synthetize asparagine from ammonia. Distinct from other eukaryotes, Trypanosoma cruzi produces an AS-A. AS-A from...
Source: Molecular and Biochemical Parasitology - March 17, 2019 Category: Parasitology Source Type: research

Characterization of strain-specific phenotypes associated with knockout of dense granule protein 9 in Toxoplasma gondii
Publication date: Available online 5 March 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Huanping Guo, Yang Gao, Honglin Jia, Paul Franck Adjou Moumouni, Tatsunori Masatani, Mingming Liu, Seung-Hun Lee, Eloiza May Galon, Jixu Li, Yongchang Li, Maria Agnes Tumwebaze, Byamukama Benedicto, Xuenan XuanAbstractToxoplasma gondii is an obligate intracellular parasite that can invade any nucleated cell of mammals and cause toxoplasmosis. Dense granule proteins play major structural functions within the parasitophorous vacuole (PV) and the cyst wall of T. gondii. Moreover, their particular location within the PV allo...
Source: Molecular and Biochemical Parasitology - March 6, 2019 Category: Parasitology Source Type: research

Immunofluorescence staining protocol for STED nanoscopy of Plasmodium-infected red blood cells
Publication date: Available online 1 March 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Ann-Kathrin Mehnert, Caroline Sophie Simon, Julien GuizettiAbstractImmunofluorescence staining is the key technique for visualizing organization of endogenous cellular structures in single cells. Labeling and imaging of blood stage Plasmodium falciparum has always been challenging since it is a small intracellular parasite. A widely-used standard for parasite immunofluorescence is fixation in suspension with addition of minute amounts of glutaraldehyde to the paraformaldehyde-based solution. While this maintains red bloo...
Source: Molecular and Biochemical Parasitology - March 5, 2019 Category: Parasitology Source Type: research

PROTEOMIC ANALYSIS OF GLYCOSOMES FROM Trypanosoma cruzi EPIMASTIGOTES
In this study, we have purified glycosomes from T. cruzi epimastigotes, collected the soluble and membrane fractions of these organelles, and separated peripheral and integral membrane proteins by Na2CO3 treatment and osmotic shock. Proteomic analysis was performed on each of these fractions, allowing us to confirm the presence of enzymes involved in various metabolic pathways as well as identify new components of this parasite’s glycosomes.Graphical abstract (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - March 5, 2019 Category: Parasitology Source Type: research

Transcriptomic profile of two developmental stages of the cestode parasite Mesocestoides corti
Publication date: Available online 21 February 2019Source: Molecular and Biochemical ParasitologyAuthor(s): T. Basika, G.P. Paludo, F.M. Araujo, A.C. Salim, F. Pais, L. Maldonado, N. Macchiaroli, J. Camargo de Lima, M. Rosenzvit, G. Oliveira, L. Kamenetzky, H.B. FerreiraAbstractCestode development involves complex morphological and physiological changes. Here, we performed a differential expression analysis of gene transcripts between two developmental stages of the model cestode Mesocestoides corti. A RNA-seq-based approach was used to compare the transcriptomes of the tetrathyridium (larval, TT) and strobilated worm (ST)...
Source: Molecular and Biochemical Parasitology - February 22, 2019 Category: Parasitology Source Type: research

Cloning, expression and purification of 3'-nucleotidase/nuclease, an enzyme responsible for the Leishmania escape from neutrophil extracellular traps
This study aims to deepen the knowledge about 3’NT/NU, mainly with respect to its nuclease activity that is little studied in the current literature. For this, we cloned, expressed and purified the recombinant La3'NT/NU and have confirmed its contribution to the parasite escape from NET-mediated killing.Graphical abstract (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - February 15, 2019 Category: Parasitology Source Type: research