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Infectious Disease: Gastroenteritis

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Total 621 results found since Jan 2013.

Wheat - Dependent Exercise-Induced Anaphylaxis Occurred With a Delayed Onset of 10 to 24 hours After Wheat Ingestion: A Case Report.
We report a case of a 51-year-old man who experienced 5 anaphylaxis attacks; two of which occurred 10 to 24 hours after wheat ingestion and exercise. He has a history of chronic gastroenteritis that responds well to antihistamine drugs but not proton pump inhibitors (PPIs) and prokinetic agents. Abdominal CT results implied the possibility of superior mesenteric artery syndrome. We suggest that WDEIA occurs 6 hours after wheat ingestion in cases compounded by obstructive gastrointestinal diseases. PMID: 24991463 [PubMed]
Source: Allergy, Asthma and Immunology Research - November 16, 2014 Category: Allergy & Immunology Tags: Allergy Asthma Immunol Res Source Type: research

Immunomodulatory Effects of Lactobacillus salivarius LS01 and Bifidobacterium breve BR03, Alone and in Combination, on Peripheral Blood Mononuclear Cells of Allergic Asthmatics.
Authors: Drago L, De Vecchi E, Gabrieli A, De Grandi R, Toscano M Abstract The aim of this study was to evaluate probiotic characteristics of Lactobacillus salivarius LS01 and Bifidobacterium breve BR03 alone and in combination and their immunomodulatory activity in asthmatic subjects. Subjects affected by allergic asthma were recruited. Initially, LS01 and BR03 were analyzed for their growth compatibility by a broth compatibility assay. To study the antimicrobial activity of probiotic strains, an agar diffusion assay was performed. Finally, cytokine production by peripheral blood mononuclear cells (PBMCs) stimulat...
Source: Allergy, Asthma and Immunology Research - March 12, 2015 Category: Allergy & Immunology Tags: Allergy Asthma Immunol Res Source Type: research

Expression and Purification of a Novel Computationally Designed Antigen for Simultaneously Detection of HTLV-1 and HBV Antibodies.
In this study, a chimeric antigen was designed using computational techniques for simultaneous detection of anti-HTLV-I and anti-HBV in infected sera. Databases were searched for amino acid sequences of HBV/HLV-I diagnostic antigens. The immunodominant fragments were selected based on propensity scales. The diagnostic antigen was designed using these fragments. Secondary and tertiary structures were predicted and the B-cell epitopes were mapped on the surface of built model. The synthetic DNA coding antigen was sub-cloned into pGS21a expression vector. SDS-PAGE analysis showed that glutathione fused antigen was highly expr...
Source: Iranian Journal of Allergy, Asthma and Immunology - March 18, 2015 Category: Allergy & Immunology Authors: Heydari Zarnagh H, Ravanshad M, Pourfatollah AA, Rasaee MJ Tags: Iran J Allergy Asthma Immunol Source Type: research

Constructing Chimeric Antigen for Precise Screening of HTLV-I Infection.
Abstract  Individual preparation of two human T-cell lymphotropic virus type I (HTLV-I) diagnostic GST fused peptides (MTA-1 and GD21) is time-consuming and expensive. The aim of this study was to design a novel single chimeric antigen (SCA) to obviate separate expression of proteins and reduce the cost of reagent preparation.Structural protein fragments, including immunodominant B cell linear epitopes, wereselected and different SCAs were designed. Tertiary structure, epitope exposure, solubility and stability were calculated for each SCA and compared with each other. The synthetic DNA  encoding  the  interes...
Source: Iranian Journal of Allergy, Asthma and Immunology - August 1, 2015 Category: Allergy & Immunology Authors: Heydari-Zarnagh H, Hassanpour K, Rasaee M Tags: Iran J Allergy Asthma Immunol Source Type: research

High-Level Soluble Expression and One-step Purification of HTLV-I P19 Protein in Escherichia coli by Fusion Expression.
This study aimed to express and purify a soluble form of recombinant HTLV-I p19 protein in an E. coli expression system. The synthetic DNA encoding the p19 was subcloned into a pGS21a vector along with a His-GST solubility/purification tag. The recombinant pGS21a-p19 vector was then transformed into chemically competent E. coli BL21 (DE3) cells, and expression of the recombinant His-GST-p19 protein was induced by IPTG. Expression and distribution of the His-GST-p19 protein in soluble and insoluble fractions were evaluated using SDS-PAGE. Antigenicity of the His-GST-p19 protein was evaluated using ELISA after purifying the ...
Source: Iranian Journal of Allergy, Asthma and Immunology - November 1, 2015 Category: Allergy & Immunology Authors: Mosadeghi P, Heydari Zarnagh H, Mohammad-Zadeh M, Salehi Moghaddam M Tags: Iran J Allergy Asthma Immunol Source Type: research

Functional and Key Gene Expression Analyses of Chicken Monocyte-derived Dendritic Cells with Recombinant Interleukin 4.
In this study full length of chicken IL-4 (cIL-4) cDNA was cloned, characterized and expressed in Escherichia coli. Subsequently, the expressed IL-4 was used to induce monocytes- derived DCs (MDDC). Typical features of DCs such as long membrane protrusions, apparently was dominant only four days after cytokine induction. Analyses of selected key genes' expression also confirmed that most of the monocytes shifted to DCs. The findings of the present study strongly suggest that the cloning and expression of cIL-4 in the bacterial host without any codon optimization or other modifications could produce mature MDDC in six to ...
Source: Iranian Journal of Allergy, Asthma and Immunology - November 30, 2016 Category: Allergy & Immunology Authors: Khajeh H, Bahari A, Lagzian M, Sabbagh SK Tags: Iran J Allergy Asthma Immunol Source Type: research

Production and Characterization of Monoclonal Antibody against Recombinant Virus Coat Protein CP42.
In this study, recombinant GFLV coat protein gene related to selected antigenic determinants was inserted into pET-28a bacterial expression vector and the construct (pET-28a CP42) was cloned into E. coli strain (DE3). Expressed protein was verified with western blotting assay by the use of commercially available anti-GFLV antibody. The recombinant protein was purified using nickel-nitrilotriacetic acid (Ni-NTA) resin. Balb/c mice were immunized with purified protein and splenocytes of hyperimmunized mice were fused with murine myeloma Sp2/0 cells. Positive hybridomas were selected by ELISA using CP42 as coating antigen. Th...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2017 Category: Allergy & Immunology Authors: Shibaei N, Majidi J, Razavi K, Karkhane AA, Sokhandan-Bashir N, Aghebati-Maleki L Tags: Iran J Allergy Asthma Immunol Source Type: research

CCR3 Monoclonal Antibody Inhibits Eosinophilic Inflammation and Mucosal Injury in a Mouse Model of Eosinophilic Gastroenteritis.
CONCLUSIONS: Anti-CCR3 antibody significantly reduced the severity of eosinophilic inflammation, mucosal injury, and diarrhea in a mouse model of food allergen-induced GI eosinophilic inflammation. CCR3 may be a novel therapeutic target for treatment of EGE and other GI eosinophil-mediated diseases. PMID: 28497923 [PubMed]
Source: Allergy, Asthma and Immunology Research - May 13, 2017 Category: Allergy & Immunology Tags: Allergy Asthma Immunol Res Source Type: research

House dust mites as potential carriers for IgE sensitisation to bacterial antigens
ConclusionHDM may serve as carriers of bacteria responsible for the induction of IgE sensitisation to microbial antigens.This article is protected by copyright. All rights reserved.
Source: Allergy - July 25, 2017 Category: Allergy & Immunology Authors: Sheron Dzoro, Irene Mittermann, Yvonne Resch, Susanne Vrtala, Marion Nehr, Alexander M. Hirschl, Gustav Wikberg, Lena Lundeberg, Catharina Johansson, Annika Scheynius, Rudolf Valenta Tags: Original Article: Experimental Allergy and Immunology Source Type: research

Lactose intolerance and gastrointestinal cow ’s milk allergy in infants and children – common misconceptions revisited
AbstractLactose is the main carbohydrate in human and mammalian milk. Lactose requires enzymatic hydrolysis by lactase into D-glucose and D-galactose before it can be absorbed. Term infants express sufficient lactase to digest about one liter of breast milk daily. Physiological lactose malabsorption in infancy confers beneficial prebiotic effects, including the establishment of Bifidobacterium-rich fecal microbiota. In many populations, lactase levels decline after weaning (lactase non-persistence; LNP). LNP affects about 70% of the world ’s population and is the physiological basis for primary lactose intolerance (LI). ...
Source: World Allergy Organization Journal - December 12, 2017 Category: Allergy & Immunology Source Type: research

Fecal microbiota profile in atopic asthmatic adult patients.
Conclusion. Atopic asthma is significantly associated with gut microbiota Lactobacilli and E. coli. It is important to determine the organism involved, to focus on microbiome-driven disease and therapies. PMID: 29479926 [PubMed - as supplied by publisher]
Source: European Annals of Allergy and Clinical Immunology - February 27, 2018 Category: Allergy & Immunology Tags: Eur Ann Allergy Clin Immunol Source Type: research

Production of Recombinant Protein of Salsola Kali (Sal k1) Pollen Allergen in Lactococcus Lactis.
This study demonstrated that L. lactis may be used as a promising live delivery system for recombinant Sal k1 protein without altering its immunoreactivity; however, its efficacy in the context of the immune system is suggested to be pursued in future studies. PMID: 29757586 [PubMed - in process]
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2018 Category: Allergy & Immunology Authors: Ghasemi Z, Varasteh AR, Moghadam M, Sedghi F, Jabbariazad F, Sankian M Tags: Iran J Allergy Asthma Immunol Source Type: research

Eosinophilic Gastrointestinal Diseases (EGIDs) beyond Eosinophilic Esophagitis (EoE).
CONCLUSIONS: With the seeming increase in all types of EGIDs, phenotypic descriptions are emerging that will likely promote better understanding of the pathogenesis and identification of novel therapeutic targets. PMID: 29940308 [PubMed - as supplied by publisher]
Source: Annals of Allergy, Asthma and Immunology - June 22, 2018 Category: Allergy & Immunology Authors: Egan M, Furuta GT Tags: Ann Allergy Asthma Immunol Source Type: research

Severe Asthma With Eosinophilic Gastroenteritis Effectively Managed by Mepolizumab and Omalizumab.
PMID: 30056148 [PubMed - as supplied by publisher]
Source: Annals of Allergy, Asthma and Immunology - July 26, 2018 Category: Allergy & Immunology Authors: Han D, Lee JK Tags: Ann Allergy Asthma Immunol Source Type: research

Infectious etiology of chronic diarrhea in patients with primary immunodeficiency diseases.
Conclusion. The current study suggests considering both usual and unusual pathogens in laboratory investigation and in the empiric treatment of chronic diarrhea. Opportunistic pathogens should be taken into account when no other pathogen is identified, especially in patients on long-term treatment or prophylaxis with antifungals/antibiotics and in those from geographical locations that favor pathogenicity of these organisms. PMID: 30417635 [PubMed - as supplied by publisher]
Source: European Annals of Allergy and Clinical Immunology - November 14, 2018 Category: Allergy & Immunology Tags: Eur Ann Allergy Clin Immunol Source Type: research