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Specialty: Orthopaedics
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Total 64 results found since Jan 2013.

Small Players Ruling the Hard Game: siRNA in Bone Regeneration
This article is protected by copyright. All rights reserved
Source: Journal of Bone and Mineral Research - February 1, 2016 Category: Orthopaedics Authors: S Ghadakzadeh, M Mekhail, A Aoude, R Hamdy, M Tabrizian Tags: Review Source Type: research

Effective knock down of matrix metalloproteinase‐13 by an intra‐articular injection of small interfering RNA (siRNA) in a murine surgically‐induced osteoarthritis model
This study investigated the effect of MMP‐13 gene knock down on cartilage degradation by injecting small interfering RNA (siRNA) into knee joints in a mouse model of osteoarthritis (OA). OA was induced in male C57BL/6 mice by destabilization of medial meniscus (DMM) surgery. Change of Mmp13 expression over time was determined by qPCR analysis from 3 days to 6 weeks after surgery. Mmp13 and control chemically modified siRNA were injected into the knee joint 1 week after surgery and expression levels were assessed in synovium by qPCR 48 h later. Cartilage degradation was histologically assessed 8 weeks after DMM surgery ...
Source: Journal of Orthopaedic Research - May 23, 2014 Category: Orthopaedics Authors: Ryuichiro Akagi, Takahisa Sasho, Masahiko Saito, Jun Endo, Satoshi Yamaguchi, Yuta Muramatsu, Shunsuke Mukoyama, Yorikazu Akatsu, Joe Katsuragi, Taisuke Fukawa, Kazuhisa Takahashi Tags: Research Article Source Type: research

Blockage of Src by Specific siRNA as a Novel Therapeutic Strategy to Prevent Destructive Repair in Steroid‐Associated Osteonecrosis in Rabbits
This study was designed to prove our hypothesis that blocking VEGF‐Src signaling pathway by specific Src siRNA was able to prevent destructive repair in a SAON rabbit model. Destructive repair in SAON was induced in rabbits. At 2, 4, 6 weeks after SAON induction, VEGF, anti‐VEGF, Src siRNA, Src siRNA + VEGF, control siRNA and saline were intramedullary injected into proximal femora for each group, respectively. Vascularization and permeability were quantified by dynamic contrast‐enhanced (DCE) MRI. At week 6 after SAON induction, proximal femora were dissected for micro‐CT‐based trabecular architecture with f...
Source: Journal of Bone and Mineral Research - April 27, 2015 Category: Orthopaedics Authors: Li‐zhen Zheng, Hui‐juan Cao, Shi‐hui Chen, Tao Tang, Wei‐min Fu, Le Huang, Dick Ho Kiu Chow, Yi‐xiang Wang, James Francis Griffith, Wei He, Hong Zhou, De‐wei Zhao, Ge Zhang, Xin‐luan Wang, Ling Qin Tags: Original Article Source Type: research

Src siRNA prevents corticosteroid-associated osteoporosis in a rabbit model
In an established steroid-associated osteonecrosis (SAON) rabbit model we found recently that blockage Src by siRNA could improve reconstructive repair of osteonecrosis via enhancing osteogenesis and inhibiting bone resorption. The current study investigated if blocking Src was able to prevent steroid-associated osteoporosis (SAOP) in the same SAON animal model. Rabbits were treated with pulsed lipopolysaccharide (LPS) and corticosteroid methylprednisolone (MPS). At 2, 4, 6weeks after induction, Src siRNA, control siRNA and saline were intramedullary injected into proximal femur, respectively.
Source: Bone - November 17, 2015 Category: Orthopaedics Authors: Li-Zhen Zheng, Xin-Luan Wang, Hui-Juan Cao, Shi-Hui Chen, Le Huang, Ling Qin Tags: Original Full Length Article Source Type: research

Small Interfering RNA-Mediated Suppression of Fas Modulate Apoptosis and Proliferation in Rat Intervertebral Disc Cells.
Conclusions: The observed dual positive effect of Fas siRNA might be a powerful therapeutic approach for disc degeneration by suppression of harmful gene expression. PMID: 29093776 [PubMed]
Source: Asian Spine Journal - November 3, 2017 Category: Orthopaedics Tags: Asian Spine J Source Type: research

Effect of RNA Interference-Mediated Suppression of p75 on the Viability of Rat Notochordal Cells.
CONCLUSIONS: siRNA-mediated suppression of p75 inhibited apoptosis and increased proliferation of notochordal cells under conditions of serum deprivation, suggesting that RNAi might serve as a novel therapeutic approach for disc degeneration caused by insufficient viability of disc cells through the suppression of the expression of harmful genes. PMID: 27994772 [PubMed]
Source: Asian Spine Journal - December 21, 2016 Category: Orthopaedics Tags: Asian Spine J Source Type: research

The effect of noggin interference in a rabbit posterolateral spinal fusion model
Conclusions Early noggin suppression does not appear to enhance the BMP activity sufficiently to significantly affect final fusion rates in our model.
Source: European Spine Journal - October 30, 2014 Category: Orthopaedics Source Type: research

Evaluation of the Effect of (S)-3,4-Dicarboxyphenylglycine as a Metabotropic Glutamate Receptors Subtype 8 Agonist on Thermal Nociception Following Central Neuropathic Pain.
Conclusions: The results revealed that activation of mGluR8 in PAG is not capable of improving the thermal hyperalgesia threshold. Based on the decreased expression of mGluR8 after SCI induced by clip compression injury and its significant increase after treatment of siRNA against mGluR8, this method might still hold promise as an effective treatment of neuropathic pain. It can be concluded that increased expression of mGluR8 is due to the fact that DCPG prevents the death of neurons that express these receptors. PMID: 32460469 [PubMed - as supplied by publisher]
Source: Asian Spine Journal - May 29, 2020 Category: Orthopaedics Tags: Asian Spine J Source Type: research

Expression of Caveolin-1 in Periodontal Tissue and Its Role in Osteoblastic and Cementoblastic Differentiation In Vitro
This study demonstrates, for the first time, that Cav-1 is expressed in developing periodontal tissue and in vitro in periodontal-related cells. Cav-1 inhibition positively regulates osteoblastic differentiation in hPDLCs, cementoblasts, and osteoblasts via BMP, AMPK, MAPK, and NF-κB pathway. Thus, Cav-1 inhibition may be a novel molecular target for therapeutic approaches in periodontitis or osteolytic disease.
Source: Calcified Tissue International - December 19, 2015 Category: Orthopaedics Source Type: research

Delta-like Factor 1 as a Possible Therapeutic Target for Sarcomas.
Conclusions: Our study showed that the DLK1 gene induces tumorigenesis in various sarcomas and is associated with invasive mechanism in sarcoma. These results suggest DLK1 could serve as a possible therapeutic target in a variety of sarcomas. PMID: 32904069 [PubMed - in process]
Source: Clinics in Orthopedic Surgery - September 11, 2020 Category: Orthopaedics Tags: Clin Orthop Surg Source Type: research

Connexin 43 is a potential regulator in fluid shear stress‐induced signal transduction in osteocytes
ABSTRACT Connexin 43 (Cx43), a gap junctional protein, regulates osteocyte viability, and modulates mechanical stimulation‐induced bone remodeling. However, the underlying mechanisms of its action remain unclear. In the current study, osteocyte‐like MLO‐Y4 cells were exposed to fluid shear stress (FSS) of 16 (physiological) or 30 (high) dyne/cm2 for the indicated time points. Cx43 gene (Gja1) was silenced using siRNA or the protein was blocked chemically. The signaling molecules related to osteocyte apoptosis, osteogenesis, or osteoclastogenesis were detected at mRNA or protein levels. The results showed that physiol...
Source: Journal of Orthopaedic Research - July 22, 2013 Category: Orthopaedics Authors: Xiaoting Li, Chenglin Liu, Ping Li, Shengnan Li, Zhihe Zhao, Yangxi Chen, Bo Huo, Ding Zhang Tags: Research Article Source Type: research

Involvement of ERCC1 in the pathogenesis of osteoarthritis through the modulation of apoptosis and cellular senescence
In this study we investigated the function of ERCC1 in chondrocytes and its association with the pathophysiology of OA. ERCC1 expression in normal and osteoarthritic cartilage was assessed, as were changes in ERCC1 expression in chondrocytes under catabolic stress. Inhibiting ERCC1 in chondrocytes under interleukin‐1β stimulation using small interfering RNA (siRNA) was also evaluated. Finally, cellular senescence and apoptosis were examined in relation to ERCC1 function. ERCC1 expression was decreased in OA cartilage and increased within 4 h of exposure to interleukin (IL)‐1β, but decreased after 12 h. The inhibi...
Source: Journal of Orthopaedic Research - June 25, 2014 Category: Orthopaedics Authors: Koji Takayama, Yohei Kawakami, Sahnghoon Lee, Nick Greco, Mitra Lavasani, Yutaka Mifune, James H. Cummins, Takashi Yurube, Ryosuke Kuroda, Masahiro Kurosaka, Freddie H. Fu, Johnny Huard Tags: Research Article Source Type: research

Down-regulated expression of vimentin induced by mechanical stress in fibroblasts derived from patients with ossification of the posterior longitudinal ligament
Conclusion The down-regulation of vimentin induced by mechanical stress plays an important role in the progression of OPLL through the induction of osteogenic differentiation in OPLL cells.
Source: European Spine Journal - October 30, 2014 Category: Orthopaedics Source Type: research

Connexin 43 and ERK Regulate Tension‐Induced Signal Transduction in Human Periodontal Ligament Fibroblasts
This article is protected by copyright. All rights reserved
Source: Journal of Orthopaedic Research - March 2, 2015 Category: Orthopaedics Authors: Shengnan Li, Huajing Zhang, Shuna Li, Yanqi Yang, Bo Huo, Ding Zhang Tags: Research Article Source Type: research

Transmission of ER stress response by ATF6 promotes endochondral bone growth
Conclusions: Our observations demonstrate that ATF6 positively regulates chondrocyte hypertrophy and endochondral bone formation through activating Runx2-mediated hypertrophic chondrocyte differentiation.
Source: Journal of Orthopaedic Surgery and Research - September 15, 2015 Category: Orthopaedics Authors: Zhangyuan XiongRong JiangPeng ZhangXiaofeng HanFeng-Jin Guo Source Type: research