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Total 146 results found since Jan 2013.
Budding uninhibited by benzimidazoles-1 (BUB1) regulates EGFR signaling by reducing EGFR internalization
Aging (Albany NY). 2023 Jul 3;15. doi: 10.18632/aging.204820. Online ahead of print.ABSTRACTEGFR signaling initiates upon ligand binding which leads to activation and internalization of the receptor-ligand complex. Here, we evaluated if BUB1 impacted EGFR signaling by regulating EGFR receptor internalization and activation. BUB1 was ablated genomically (siRNA) or biochemically (2OH-BNPP1) in cells. EGF ligand was used to initiate EGFR signaling while disuccinimidyl suberate (DSS) was used for cross linking cellular proteins. EGFR signaling was measured by western immunoblotting and receptor internalization was evaluated by...
Source: Aging - July 3, 2023 Category: Biomedical Science Authors: Shyam Nyati Grant Young Corey Speers Mukesh K Nyati Alnawaz Rehemtulla Source Type: research
Conserved NIMA kinases regulate multiple steps of endocytic trafficking
by Braveen B. Joseph, Naava Naslavsky, Shaonil Binti, Sylvia Conquest, Lexi Robison, Ge Bai, Rafael O. Homer, Barth D. Grant, Steve Caplan, David S. Fay
Human NIMA-related kinases have primarily been studied for their roles in cell cycle progression (NEK1/2/6/7/9), checkpoint –DNA-damage control (NEK1/2/4/5/10/11), and ciliogenesis (NEK1/4/8). We previously showed thatCaenorhabditis elegans NEKL-2 (NEK8/9 homolog) and NEKL-3 (NEK6/7 homolog) regulate apical clathrin-mediated endocytosis (CME) in the worm epidermis and are essential for molting. Here we show that NEKL-2 and NEKL-3 also have distinct roles in controlling ...
Source: PLoS Genetics - April 26, 2023 Category: Genetics & Stem Cells Authors: Braveen B. Joseph Source Type: research
Abstracts of Presentations at the Association of Clinical Scientists 143 < sup > rd < /sup > Meeting Louisville, KY May 11-14,2022
Conclusion: These assays are suitable for routine diagnostic. The UltraFast NextGenPCR is the fastest with average time (30mins), followed by Agilent (2 hrs) and MassArray (6hrs). Upon completion of this activity, participants should be able to examine, measure and compare results from different assays for SARS detection, evaluate and diagnose accurately, as well as being able to plan, organize and recommend a diagnostic procedure for diagnostic laboratory. Key words: SARS-CoV-2, RNA extraction, RT-PCR, limit of detection, quantification cycle, COVID-19, in vitro diagnostic tests, Agilent, Massarray, Ultrafast. [20] From t...
Source: Annals of Clinical and Laboratory Science - July 1, 2022 Category: Laboratory Medicine Source Type: research
Transcriptomic and small RNA response to Mayaro virus infection in < i > Anopheles stephensi < /i > mosquitoes
In this study we detail the transcriptomic and small RNA responses ofAn.stephensi to infection with MAYV via infectious bloodmeal at 2, 7, and 14 days post infection (dpi). 487 unique transcripts were significantly regulated, 78 putative novel miRNAs were identified, and an siRNA response is observed targeting the MAYV genome. Gene ontology analysis of transcripts regulated at each timepoint shows a number of proteases regulated at 2 and 7 dpi, potentially representative of Toll or melanization pathway activation, and repression of pathways related to autophagy and apoptosis at 14 dpi. These findings provide a basic unders...
Source: PLoS Neglected Tropical Diseases - June 28, 2022 Category: Tropical Medicine Authors: Cory Henderson Source Type: research
Silencing of NILCO by Small Interfering RNA Effects MDR1 in Colon Cancer Cells
In conclusion, knockdown of NILCO genes is effective in regulating MDR1 expression, which is a hallmark in chemoresistance. The present study was supported by Eskisehir Osmangazi University Scientific Research Projects (BAP) Coordination Unit (grant no. 202011D11).PMID:35557372 | DOI:10.1096/fasebj.2022.36.S1.L7435
Source: Cancer Control - May 13, 2022 Category: Cancer & Oncology Authors: Nilufer Erkasap Aysel Gultekin Rumeysa Ozyurt Mete Ozkurt Source Type: research
The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Plays an Important Role in Fetal Human Colon Cell Migration and Proliferation
CONCLUSION-: Our results indicate that CFTR plays an important role in FHC colon cell migration and EGFR signaling, which could lead to a better understanding as to why CF patients have increased risk for colon cancer.PMID:35556394 | DOI:10.1096/fasebj.2022.36.S1.R6139
Source: Cancer Control - May 13, 2022 Category: Cancer & Oncology Authors: Cara A Mogan Garett J Grant Kaylee LeBaron Theodore G Liou My N Helms Source Type: research
