Transcriptomic and small RNA response to Mayaro virus infection in < i > Anopheles stephensi < /i > mosquitoes

In this study we detail the transcriptomic and small RNA responses ofAn.stephensi to infection with MAYV via infectious bloodmeal at 2, 7, and 14 days post infection (dpi). 487 unique transcripts were significantly regulated, 78 putative novel miRNAs were identified, and an siRNA response is observed targeting the MAYV genome. Gene ontology analysis of transcripts regulated at each timepoint shows a number of proteases regulated at 2 and 7 dpi, potentially representative of Toll or melanization pathway activation, and repression of pathways related to autophagy and apoptosis at 14 dpi. These findings provide a basic understanding of the infection response ofAn.stephensi to MAYV and help to identify host factors which might be useful to target to inhibit viral replication inAnopheles mosquitoes.

Source: PLoS Neglected Tropical Diseases - June 28, 2022 Category: Tropical Medicine Authors: Cory Henderson Source Type: research