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Specialty: Biomedical Science
Drug: Insulin

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Total 20 results found since Jan 2013.

Doxorubicin induced ROS-dependent HIF1 α activation mediates blockage of IGF1R survival signaling by IGFBP3 promotes cardiac apoptosis
In this study, we investigated the role of IGFBP3, HIF1A, and PHD in Dox-induced cardiac apoptosis. Cells challenged with 1 μM Dox for 24 h increased ROS generation, augmented intracellular and secreted IGFBP3 levels, and reduced IGF1 signaling. Further, we showed that Dox enhanced the extracellular association of IGF1 with IGFBP3. Moreover, echocardiography parameters, especially ejection fraction (EF) and fractional shortening (FS) were significantly reduced in ventricle tissue of Dox challenged rats. Notably, siRNA approach against IGFBP3 or an anti-IGFBP3 antibody rescued Dox-induced cardiac apoptosis, mitochondrial R...
Source: Aging - January 5, 2023 Category: Biomedical Science Authors: Su-Ying Wen Ayaz Ali I-Chieh Huang Jian-Sheng Liu Po-Yuan Chen Vijaya Padma Viswanadha Chih-Yang Huang Wei-Wen Kuo Source Type: research

Downregulation of IGFBP5 contributes to replicative senescence via ERK2 activation in mouse embryonic fibroblasts
In this study, we investigated the role of IGFBP5 in replicative senescence in embryonic mouse fibroblasts (MEFs). During passages according to the 3T3 method, MEFs underwent senescence after the 5th passage (P5) based on cell growth arrest, an increase in the number of cells positive for senescence-associated β-galactosidase (SA-β-GAL) staining, and upregulation of p16 and p19. In P8 MEFs, IGFBP5 mRNA level was markedly reduced compared with that in P2 MEFs. Downregulation of IGFBP5 via siRNA in P2 MEFs increased the number of SA-β-GAL-positive cells, upregulated p16 and p19, and inhibited cell growth. Incubation of ME...
Source: Aging - April 4, 2022 Category: Biomedical Science Authors: Iyori Nojima Ryusuke Hosoda Yuki Toda Yoshiki Saito Naohiro Ueda Kouhei Horimoto Naotoshi Iwahara Yoshiyuki Horio Atsushi Kuno Source Type: research

High dose insulin promotes the proliferation of vascular smooth muscle cells via AP-1/ SM- α pathway
This study aims to investigate the effects of insulin on VSMC. Insulin was used to stimulate rat VSMCs, and the effects on cell cycle and proliferation were subsequently analyzed using flow cytometry. Furthermore, AP-1 and SM-α overexpression vectors were constructed and transfected into VSMCs. AP-1 and SM-α were inhibited by SR11302 and SM-α siRNA, respectively. The mRNA and protein expression levels were subsequently detected using the reversetranscription quantitative polymerase chain reaction and western blotting, respectively. AP-1 and SM-α gene promoter binding sites were determined using luciferase and chromatin...
Source: Journal of Biological Regulators and Homeostatic Agents - June 22, 2021 Category: Biomedical Science Authors: Y Peng P Cai S F Zou M Jia W T Zhong Y Wang X K Wang Source Type: research

Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3 α.
Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3α. Biosci Rep. 2019 Oct 30;39(10): Authors: Wang Y, Gong W, Lv S, Qu H, He Y Abstract Methylation of hypoxia-inducible factor-3α (HIF3A) was previously demonstrated to be highly associated with insulin resistance (IR) in patients with gestational diabetes mellitus (GDM). We aimed to study the therapeutic effects of Berberine (BBR) on GDM and the possible mechanisms. The expressions and methylated states of HIF3A in pregnant women with GDM were compared with that in healthy controls. T...
Source: Bioscience Reports - October 29, 2019 Category: Biomedical Science Authors: Wang Y, Gong W, Lv S, Qu H, He Y Tags: Biosci Rep Source Type: research

Integrated proximal proteomics reveals IRS2 as a determinant of cell survival in ALK-driven neuroblastoma.
Abstract Oncogenic anaplastic lymphoma kinase (ALK) is one of the few druggable targets in neuroblastoma, and therapy resistance to ALK-targeting tyrosine kinase inhibitors (TKIs) comprises an inevitable clinical challenge. Therefore, a better understanding of the oncogenic signaling network rewiring driven by ALK is necessary to improve and guide future therapies. Here, we performed quantitative mass spectrometry-based proteomics on neuroblastoma cells treated with one of three clinically relevant ALK TKIs (crizotinib, LDK378, or lorlatinib) or an experimentally used ALK TKI (TAE684) to unravel aberrant ALK signa...
Source: Science Signaling - November 20, 2018 Category: Biomedical Science Authors: Emdal KB, Pedersen AK, Bekker-Jensen DB, Lundby A, Claeys S, De Preter K, Speleman F, Francavilla C, Olsen JV Tags: Sci Signal Source Type: research

Transcriptomic analyses reveal rhythmic and CLOCK-driven pathways in human skeletal muscle
Circadian regulation of transcriptional processes has a broad impact on cell metabolism. Here, we compared the diurnal transcriptome of human skeletal muscle conducted on serial muscle biopsies in vivo with profiles of human skeletal myotubes synchronized in vitro. More extensive rhythmic transcription was observed in human skeletal muscle compared to in vitro cell culture as a large part of the in vivo mRNA rhythmicity was lost in vitro. siRNA-mediated clock disruption in primary myotubes significantly affected the expression of ~8% of all genes, with impact on glucose homeostasis and lipid metabolism. Genes involved in G...
Source: eLife - April 16, 2018 Category: Biomedical Science Tags: Human Biology and Medicine Source Type: research

CREG1 heterozygous mice are susceptible to high fat diet-induced obesity and insulin resistance
This study uncovered a novel function of CREG1 in metabolic disorders.
Source: PLoS One - May 1, 2017 Category: Biomedical Science Authors: Xiaoxiang Tian Source Type: research

Dual effects of fructose on ChREBP and FoxO1/3{alpha} are responsible for AldoB upregulation and vascular remodeling
Increased production of methylglyoxal (MG) in vascular tissues is one of the causative factors for vascular remodeling in different subtypes of metabolic syndrome, including hypertension and insulin resistance. Fructose-induced upregulation of aldolase B (AldoB) contributes to increased vascular MG production but the underlying mechanisms have been unclear. Serum levels of MG and fructose were determined in diabetic patients with hypertension. MG level had significant positive correlations with blood pressure and fructose level respectively. C57Bl/6 mice were fed with control or fructose-enriched diet for 3 months and ultr...
Source: Clinical Science - December 21, 2016 Category: Biomedical Science Authors: Cao, W., Chang, T., Li, X.-q., Wang, R., Wu, L. Tags: PublishAheadOfPrint Source Type: research

Reserpine requires the D2-type receptor, dop-3, and the exoribonuclease, eri-1, to extend the lifespan in C. elegans.
Abstract Lifespan extension is an all systems encompassing event. Involvement of reduced insulin/IGF1 signalling is well worked out, first in the model organism Caenorhbaditis elegans followed by other systems including humans. But the role of neuronal component in lifespan extension is not well understood due to the refractory nature of neurons to small RNA interference (sRNAi) in C. elegans. Earlier, we have demonstrated that an antihypertensive drug, reserpine, extends lifespan through modulation of neurotransmitter release, especially, acetylcholine, in C. elegans. Intriguingly, the reserpine mediated lifespan...
Source: Journal of Biosciences - November 30, 2016 Category: Biomedical Science Authors: Saharia K, Kumar R, Gupta K, Mishra S, Subramaniam JR Tags: J Biosci Source Type: research

A Convenient < i > In Vivo < /i > Model Using Small Interfering RNA Silencing to Rapidly Assess Skeletal Gene Function
by Wen Zhang, Can Liu, Bao Hai, Guohong Du, Hong Wang, Huijie Leng, Yingsheng Xu, Chunli Song It is difficult to study bone in vitro because it contains various cell types that engage in cross-talk. Bone biologically links various organs, and it has thus become increasingly evident that skeletal physiology must be studied in an integrative manner in an intact animal. We developed a model u sing local intraosseous small interfering RNA (siRNA) injection to rapidly assess the effects of a target gene on the local skeletal environment. In this model, 160-g male Sprague-Dawley rats were treated for 1–2 weeks. The left tibia...
Source: PLoS One - November 27, 2016 Category: Biomedical Science Authors: Wen Zhang Source Type: research

Ampelopsin Improves Insulin Resistance by Activating PPARγ and Subsequently Up-Regulating FGF21-AMPK Signaling Pathway
by Yong Zhou, Ying Wu, Yu Qin, Lei Liu, Jing Wan, Lingyun Zou, Qianyong Zhang, Jundong Zhu, Mantian Mi Ampelopsin (APL), a major bioactive constituent of Ampelopsis grossedentata, exerts a number of biological effects. Here, we explored the anti-diabetic activity of APL and elucidate the underlying mechanism of this action. In palmitate-induced insulin resistance of L6 myotubes, APL treatment markedly up- regulated phosphorylated insulin receptor substrate-1 and protein kinase B, along with a corresponding increase of glucose uptake capacity. APL treatment also increased expressions of fibroblast growth factor (FGF21) and...
Source: PLoS One - July 7, 2016 Category: Biomedical Science Authors: Yong Zhou Source Type: research