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Specialty: Reproduction Medicine
Condition: Pregnancy
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Total 71 results found since Jan 2013.
High concentraction of taurocholic acid induced apoptosis in HTR-8/SVneo cells via overexpression of ERp29 and activation of p38
Abstract: Introduction: Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific disease associated with a significant risk of fetal complications. Our previous study using an iTRAQ-based proteomics approach showed that ERp29 was overexpressed in the placenta tissue of ICP patients, which was an apoptosis-related protein and has not been investigated in the pathogenesis of ICP. The aim of this study was to explore the role of ERp29 in the mechanism of apoptosis in the placenta of ICP.Methods: HTR-8/SVneo cells were cultured and treated with different concentrations of taurocholic acid (TCA) (0, 10, 50 and 100Â Î...
Source: Placenta - April 28, 2014 Category: Reproduction Medicine Authors: T. Zhang, C. Zhao, L. Luo, J. Xiang, J. Cheng, T. Wang, D. Chen Tags: Signaling Source Type: research
Extravillous Trophoblast-Associated ADAM12 Exerts Pro-Invasive Properties, Including Induction of Integrin Beta 1-Mediated Cellular Spreading.
Abstract
ADAM12, consisting of a membrane-bound (ADAM12L) and secreted (ADAM12S) form, is exclusively expressed in regenerating and developing tissue as well as in certain cancer types. Strong ADAM12 expression levels have been noticed in the human placenta and deregulated ADAM12S levels were associated with various pregnancy-related disorders including preeclampsia and intrauterine growth restriction. However, the role of ADAM12 in trophoblast motility has not been investigated so far. Hence, the present study aimed to investigate the specific function of the protease using different primary trophoblast cell mode...
Source: Reproductive Biology - April 2, 2014 Category: Reproduction Medicine Authors: Biadasiewicz K, Fock V, Dekan S, Proestling K, Velicky P, Haider S, Knöfler M, Fröhlich C, Pollheimer J Tags: Biol Reprod Source Type: research
MicroRNA Let-7a and dicer are important in the activation and implantation of delayed implanting mouse embryos
STUDY QUESTION
Does Let-7a have a functional role in modulating dicer expression to activate dormant mouse blastocysts for implantation?
SUMMARY ANSWER
Let-7a post-transcriptionally regulates dicer expression altering microRNA expression to affect the implantation competency of the activated blastocysts.
WHAT IS KNOWN ALREADY
The Let-7a microRNA is up-regulated during blastocyst dormancy and its forced-expression suppresses embryo implantation in vitro and in vivo. Dicer is a Let-7 target, which processes pre-microRNA to mature microRNA.
STUDY DESIGN, SIZE, DURATION
The effects on the expression of Let-7a and dicer in d...
Source: Human Reproduction - March 11, 2014 Category: Reproduction Medicine Authors: Cheong, A. W. Y., Pang, R. T. K., Liu, W.-M., Kottawatta, K. S. A., Lee, K.-F., Yeung, W. S. B. Tags: Reproductive biology Source Type: research
Leptin-Promoted Human Extravillous Trophoblasts Invasion Is MMP14 Dependent and Requires the Crosstalk Between Notch1 and PI3K/Akt Signaling.
Abstract
The over-expression of leptin is a crucial feature for the maintenance of pregnancy. The effects of leptin on trophoblasts invasion are important to its reproductive function, but the underlying mechanisms remain poorly understood. MMP14 is a member of matrix metalloproteinase (MMP) family that is closely involved in the invasion process. Here, we characterized the importance of MMP14 in the pro-invasion effect of leptin on EVT cells and elucidated its molecular mechanisms. Transwell assay revealed that leptin promoted invasion of the immortalized EVT cell line HTR-8/SVneo in a dose- and time-related fash...
Source: Biology of Reproduction - February 26, 2014 Category: Reproduction Medicine Authors: Wang H, Cheng H, Shao Q, Dong Z, Xie Q, Zhao L, Wang Q, Kong B, Qu X Tags: Biol Reprod Source Type: research
Extravillous trophoblast cell invasion is promoted by the CD44âhyaluronic acid interaction
Conclusions: The discovery of a CD44-HA interaction between EVT cells and the extracellular matrix contributes to our understanding of the mechanism underlying invasion by EVT cells.
Source: Placenta - January 17, 2014 Category: Reproduction Medicine Authors: H. Takahashi, T. Takizawa, S. Matsubara, A. Ohkuchi, T. Kuwata, R. Usui, H. Matsumoto, Y. Sato, H. Fujiwara, A. Okamoto, M. Suzuki, T. Takizawa Tags: Invasion Source Type: research
Identification of CD147 (basigin) as a mediator of trophoblast functions
STUDY QUESTION
Does CD147 regulate trophoblast functions in vitro?
SUMMARY ANSWER
CD147 exists as a receptor complex on human trophoblast and regulates the implantation, invasion and differentiation of trophoblast.
WHAT IS KNOWN ALREADY
CD147 is a membrane protein implicated in a variety of physiological and pathological conditions due to its regulation of cell–cell recognition, cell differentiation and tissue remodeling. Reduced placental CD147 expression is associated with pre-eclampsia, but the mechanism of actions remains unclear.
STUDY DESIGN, SIZE, DURATION
A loss of function approach or functional blocking ...
Source: Human Reproduction - October 11, 2013 Category: Reproduction Medicine Authors: Lee, C.-L., Lam, M. P. Y., Lam, K. K. W., Leung, C. O. N., Pang, R. T. K., Chu, I. K., Wan, T. H. L., Chai, J., Yeung, W. S. B., Chiu, P. C. N. Tags: Early pregnancy Source Type: research
Small RNA regulation of reproductive function
Abstract
Postâtranscriptional gene regulation is one mechanism that occurs âabove the genomeâ, allowing the cells of an organism to have dramatically different phenotypes and functions. Nonâcoding ribonucleic acid (ncRNA) molecules regulate transcript and protein abundance above the level of transcription, and appear to play substantial roles in regulation of reproductive tissues. Three primary classes of small ncRNA are microRNA (miRNA), endogenous small interfering RNA (endoâsiRNA), and PIWIâinteracting RNA (piRNA). These RNA classes have similarities and clear distinctions between their biogenesis and in the...
Source: Molecular Reproduction and Development - October 1, 2013 Category: Reproduction Medicine Authors: Benjamin J. Hale, CaiâXia Yang, Jason W. Ross Tags: Review Article Source Type: research
Oncostatin M stimulates cell migration and proliferation by down-regulating E-cadherin in HTR8/SVneo cell line through STAT3 activation.
CONCLUSIONS: This study suggests that OSM stimulates the migration and proliferation of EVTs during the first trimester of pregnancy through the down-regulation of E-cadherin. In addition, this study suggests that the effects of OSM on migration and proliferation are related to STAT3 activation, which is important in trophoblast invasiveness.
PMID: 24060241 [PubMed - as supplied by publisher]
Source: Reproductive Biology - September 24, 2013 Category: Reproduction Medicine Authors: Ko HS, Choi SK, Kang HK, Kim HS, Jeon JH, Park IY, Shin JC Tags: Reprod Biol Endocrinol Source Type: research
Sphingosine-1-phosphate (S1P)ïŒS1P receptor signaling regulates EVT cell invasion
Sphingosine-1-phosphate (S1P) is known as a sphingolipid involved in a cell invasion and proliferation in the several tissues. S1P has been reported to play a role in the labor onset through the COX-2 induction. However, S1P-S1PR functions during the pregnancy remains largely unknown. We aimed to study the role of S1P and it receptors in the extravillous trophoblast cells (EVT). S1P receptor (S1PR) 1-5 and sphingosine kinase 1 (SPHK1) expressed in the EVT cell lines which were HTR8/SVneo, Hch-Epc and Swan71. ATP-binding cassette ABCC1 which involved in the release of S1P, expressed in these EVT cell lines. These result ind...
Source: Placenta - September 18, 2013 Category: Reproduction Medicine Authors: Rika Miki, Tomoni Kotani, Seiji Sumigama, Hiroyuki Tsuda, Yukio Mano, Fumitaka Kikkawa Tags: Abstracts Source Type: research
A Novel Role for FOXO3 in Human Labor: Increased Expression in Laboring Myometrium, and Regulation of Pro-Inflammatory and Pro-Labor Mediators in Pregnant Human Myometrial Cells.
Abstract
Preterm birth is the leading factor causing neonatal mortality and morbidity. Inflammation plays a central role in stimulating uterine contractility, which is responsible for approximately one-third of all preterm births. Recent studies have shown that the transcription factor Forkhead box O3 (FOXO3) regulates inflammation in non-gestational tissues such as adipocytes and hepatocytes. Thus, in this study, we sought to determine the effect of (i) human term labor on myometrial FOXO3 expression, and (ii) FOXO3 inhibition and FOXO3 overexpression on pro-inflammatory and pro-labor mediators in human myometria...
Source: Biology of Reproduction - May 1, 2013 Category: Reproduction Medicine Authors: Lim R, Barker G, Lappas M Tags: Biol Reprod Source Type: research
