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The lncRNA HNF1A-AS1 is a negative prognostic factor and promotes tumorigenesis in osteosarcoma.
In this study, we detected the HNF1A-AS1 levels in human osteosarcoma tissues and cell lines by quantitative real-time polymerase chain reaction (qRT-PCR), and investigated its role in osteosarcoma by using in vitro assays. Our study showed that HNF1A-AS1 expression was significantly up-regulated in human osteosarcoma tissues and cell lines compared with their normal counterparts, and its expression level was positively correlated with the distance metastasis (P = 0.009) and tumour stage (P = 0.019). Moreover, Kaplan-Meier curves with the log-rank test showed that higher expression of HNF1A-AS1 conferred a significantly po...
Source: J Cell Mol Med - September 2, 2017 Category: Molecular Biology Authors: Cai L, Lv J, Zhang Y, Li J, Wang Y, Yang H Tags: J Cell Mol Med Source Type: research

The lncRNA HNF1A ‐AS1 is a negative prognostic factor and promotes tumorigenesis in osteosarcoma
In this study, we detected the HNF1A‐AS1 levels in human osteosarcoma tissues and cell lines by quantitative real‐time polymerase chain reaction (qRT‐PCR), and investigated its role in osteosarcoma by using in vitro assays. Our study showed that HNF1A‐AS1 expression was significantly up‐regulated in human osteosarcoma tissues and cell lines compared with their normal counterparts, and its expression level was positively correlated with the distance metastasis (P = 0.009) and tumour stage (P = 0.019). Moreover, Kaplan–Meier curves with the log‐rank test showed that higher expression of HNF1A‐AS1 conferred a ...
Source: Journal of Cellular and Molecular Medicine - September 1, 2017 Category: Molecular Biology Authors: Lijun Cai, Jinhan Lv, Yinquan Zhang, Junhong Li, Yinong Wang, Huilin Yang Tags: Original Article Source Type: research

A single nucleotide polymorphism in the 3'-untranslated region of the KRAS gene disrupts the interaction with let-7a and enhances the metastatic potential of osteosarcoma cells.
In this study, we confirmed that KRAS is a target of let-7a in OS cells, and the introduction of rs61764370 minor allele into KRAS 3'-UTR significantly compromised the microRNA (miRNA)/mRNA interaction using a luciferase reporter system. Additionally, a total of 36 OS tissue samples of three different genotypes (TT,22; TG,10; GG,4) were obtained, and the expression of let-7a and KRAS was determined. We showed that let-7a mRNA expression was similar between each group whereas the mRNA and protein expression of KRAS in the TT genotype group was significantly lower than that in the GT or GG genotype groups. Moreover, we ide...
Source: International Journal of Molecular Medicine - July 3, 2016 Category: Molecular Biology Authors: Zhang S, Hou C, Li G, Zhong Y, Zhang J, Guo X, Li B, Bi Z, Shao M Tags: Int J Mol Med Source Type: research

TRIM59 is upregulated and promotes cell proliferation and migration in human osteosarcoma.
Authors: Liang J, Xing D, Li Z, Shen J, Zhao H, Li S Abstract Osteosarcoma is a prevalent type of cancer and has a high metastatic ability, particularly for metastasis to the lungs. Effective treatment strategies have improved, however, the detailed molecular mechanism underlying the onset of this malignancy remains to be fully elucidated. The current study investigated the role of the tripartite motif (TRIM) family protein TRIM59 in osteosarcoma growth and metastasis. It was identified that TRIM59 was overexpressed in clinical osteosarcoma tissues and cultured osteosarcoma cell lines. In addition, the MTT assay de...
Source: Molecular Medicine Reports - April 30, 2016 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

CREB-regulated transcription coactivator 1 enhances CREB-dependent gene expression in spinal cord to maintain the bone cancer pain in mice
Conclusions Upregulation of CRTC1 enhancing CREB-dependent gene transcription in spinal cord may play an important role in bone cancer pain. Inhibition of spinal CRTC1 expression reduced bone cancer pain. Interruption to the positive feedback circuit between CREB/CRTC1 and its targets may contribute to the analgesic effects. These findings may provide further insight into the mechanisms and treatment of bone cancer pain.
Source: Molecular Pain - April 7, 2016 Category: Molecular Biology Authors: Liang, Y., Liu, Y., Hou, B., Zhang, W., Liu, M., Sun, Y.-E., Ma, Z., Gu, X. Tags: Research Article Source Type: research

Resveratrol abrogates the effects of hypoxia on cell proliferation, invasion and EMT in osteosarcoma cells through downregulation of the HIF-1α protein.
In this study, we investigated the role of resveratrol on regulating proliferation and invasion of osteosarcoma cells under hypoxic conditions. Saos-2 cells were cultured under controlled hypoxic conditions (3% O2) or normoxic conditions. Resveratrol (50 µM) was added in the medium, and hypoxia inducible factor-1α (HIF-1α) siRNA was used to inhibit HIF-1α transcription. Proliferation of Saos-2 cells was evaluated by the methabenzthiazuron (MTT) assay. The invasive ability of Saos-2 cells was determined by a Transwell assay. HIF-1α, E-cadherin and vimentin protein levels were detected by western blot analysis. HIF-1�...
Source: Molecular Medicine - November 10, 2014 Category: Molecular Biology Authors: Sun Y, Wang H, Liu M, Lin F, Hua J Tags: Mol Med Rep Source Type: research

Silencing of Barkor/ATG14 sensitizes osteosarcoma cells to cisplatin‑induced apoptosis.
Abstract Although surgical excision following neoadjuvant chemotherapy has contributed to the long-term survival of osteosarcoma patients, patients that do not respond to commonly used drugs including cisplatin, have a poor prognosis. Autophagy is important in the inhibition of chemotherapeutic apoptosis. Therefore, we investigated whether knockdown of Beclin1-associated autophagy-related key regulator (Barkor/ATG14) promoted cisplatin-induced apoptosis in a drug-resistant osteosarcoma cell line in vitro. Saos-2 cells were transfected with Barkor siRNA. Sensitivity of the Barkor siRNA-transfected cell line to ci...
Source: International Journal of Molecular Medicine - December 9, 2013 Category: Molecular Biology Authors: Zhao Z, Tao L, Shen C, Liu B, Yang Z, Tao H Tags: Int J Mol Med Source Type: research

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