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HOXB5 induces invasion and migration through direct transcriptional up-regulation of {beta}-catenin in human gastric carcinoma
HOX (homeobox) genes encode a family of transcriptional regulators, which have an important role in morphogenesis and differentiation during embryonic development. Their deregulated expression is involved in the carcinogenesis of many human solid tumours. In the present study, we show that HOXB5 mRNA was significantly overexpressed in gastric cancer tissues compared with adjacent normal tissues. HOXB5-up-regulated cancer cells showed increased invasion and migration activity, but no change in proliferation activity, whereas HOXB5-down-regulated cells showed decreased invasion and migration activity. Up-regulation of HOXB5 ...
Source: Biochemical Journal - November 27, 2015 Category: Biochemistry Authors: Hong, C.-S., Jeong, O., Piao, Z., Guo, C., Jung, M.-R., Choi, C., Park, Y.-K. Tags: Cell Research articles Source Type: research

Silencing Estrogen Receptor-β with siRNA in Cultured Cells
Estrogen receptors α and β (ERα and ERβ) are the two genomic estrogen receptors. ERβ was the second of these receptors to be discovered; its structure is similar to that of ERα but they are different in histological distribution. However, the functions of ERα versus ERβ are still unclear. The ability of small interfering RNAs (siRNAs) to silence gene expression has proven to be invaluable for studying gene function in cultured mammalian cells. This chapter describes the use of siRNA to inhibit the expression of ERβ in renal cell carcinoma (RCC) and to further the understandi...
Source: Springer protocols feed by Biochemistry - October 12, 2015 Category: Biochemistry Source Type: news

Silencing Estrogen Receptor- & beta; with siRNA in Cultured Cells
Estrogen receptors & alpha; and & beta; (ER & alpha; and ER & beta;) are the two genomic estrogen receptors. ER & beta; was the second of these receptors to be discovered; its structure is similar to that of ER & alpha; but they are different in histological distribution. However, the functions of ER & alpha; versus ER & beta; are still unclear. The ability of small interfering RNAs (siRNAs) to silence gene expression has proven to be invaluable for studying gene function in cultured mammalian cells. This chapter describes the use of siRNA to inhibit the expression of ER & beta; in renal cell carcinoma (RCC) and to further...
Source: Springer protocols feed by Biochemistry - October 11, 2015 Category: Biochemistry Source Type: news

Endoplasmic reticulum stress mediates the arsenic trioxide-induced apoptosis in human hepatocellular carcinoma cells.
Abstract Arsenic trioxide has been proven to trigger apoptosis in human hepatocellular carcinoma cells. And endoplasmic reticulum stress has been known to be involved in apoptosis through the induction of CCAAT/enhancer-binding protein homologous protein. However, it is unknown whether endoplasmic reticulum stress mediates arsenic trioxide-induced apoptosis in human hepatocellular carcinoma cells. Our data showed that arsenic trioxide significantly induced apoptosis in human hepatocellular carcinoma cells. Furthermore, arsenic trioxide triggered endoplasmic reticulum stress, as indicated by endoplasmic reticulum d...
Source: The International Journal of Biochemistry and Cell Biology - September 24, 2015 Category: Biochemistry Authors: Zhang XY, Yang SM, Zhang HP, Yang Y, Sun SB, Chang JP, Tao XC, Yang TY, Liu C, Yang YM Tags: Int J Biochem Cell Biol Source Type: research

HtrA1 regulates Epithelial-mesenchymal transition in hepatocellular carcinoma.
CONCLUSION: HtrA1 expression was closely related to EMT, which might be a potential mechanism underlying metastasis of HCC. PMID: 26403966 [PubMed - as supplied by publisher]
Source: Biochemical and Biophysical Research communications - September 21, 2015 Category: Biochemistry Authors: Zhu F, Duan YF, Bao WY, Liu WS, Yang Y, Cai HH Tags: Biochem Biophys Res Commun Source Type: research

HCV core protein promotes hepatocyte proliferation and chemoresistance by inhibiting NR4A1.
This study investigated the effect of HCV core protein on the proliferation of hepatocytes and hepatocellular carcinoma cells (HCC), the influence of HCV core protein on HCC apoptosis induced by the chemotherapeutic agent cisplatin, and the mechanism through which HCV core protein acts as a potential oncoprotein in HCV-related HCC by measuring the levels of NR4A1 and Runt-related transcription factor 3 (RUNX3), which are associated with tumor suppression and chemotherapy resistance. In the present study, PcDNA3.1-core and RUNX3 siRNA were transfected into LO2 and HepG2 cells using Lipofectamine 2000. LO2-core, HepG2-core, ...
Source: Biochemical and Biophysical Research communications - September 18, 2015 Category: Biochemistry Authors: Tan Y, Li Y Tags: Biochem Biophys Res Commun Source Type: research

Stanniocalcin 2 promotes cell proliferation and cisplatin resistance in cervical cancer.
Abstract Cervical cancer is one of the most common carcinomas in the female reproductive system. Treatment of cervical cancer involves surgical removal and chemotherapy. Resistance to platinum-based chemotherapy drugs including cisplatin has increasingly become an important problem in the treatment of cervical cancer patients. We found in this study that stanniocalcin 2 (STC2) expression was upregulated in both cervical cancer tissues and cell lines. The levels of STC2 expression in cervical cancer cell lines were positively correlated with the rate of cell proliferation. Furthermore, in cisplatin resistant cervic...
Source: Biochemical and Biophysical Research communications - September 7, 2015 Category: Biochemistry Authors: Wang Y, Gao Y, Cheng H, Yang G, Tan W Tags: Biochem Biophys Res Commun Source Type: research

Silencing SOX2 Expression by RNA Interference Inhibits Proliferation, Invasion and Metastasis, and Induces Apoptosis through MAP4K4/JNK Signaling Pathway in Human Laryngeal Cancer TU212 Cells
SRY (sex determining region Y)-box 2 (SOX2) plays an important role in tumor cell metastasis and apoptosis. Laryngeal squamous cell carcinoma (LSCC), responsible for 1.5% of all cancers, is one of the most common head and neck malignancies. Accumulating evidence shows that SOX2 is overexpressed in several human tumors, including lung cancer, esophageal carcinoma, pancreatic carcinoma, breast cancer, ovarian carcinoma and glioma. Our study aimed to investigate the silencing effects of SOX2 expression using RNA interference (RNAi) on various biological processes in laryngeal cancer TU212 cells, including proliferation, apopt...
Source: Journal of Histochemistry and Cytochemistry - August 25, 2015 Category: Biochemistry Authors: Yang, N., Wang, Y., Hui, L., Li, X., Jiang, X. Tags: Articles Source Type: research

TRAIL‐Induced Caspase Activation is a Prerequisite for Activation of the Endoplasmic Reticulum Stress‐Induced Signal Transduction Pathways
This article is protected by copyright. All rights reserved
Source: Journal of Cellular Biochemistry - July 24, 2015 Category: Biochemistry Authors: Dae‐Hee Lee, Ki Sa Sung, Zong Sheng Guo, William Taehyung Kwon, David L. Bartlett, Sang Cheul Oh, Yong Tae Kwon, Yong J. Lee Tags: Article Source Type: research

Knockdown of NF‐κB p65 subunit expression suppresses growth of nude mouse lung tumour cell xenografts by inhibition of Bcl‐2 apoptotic pathway
This study aimed to assess the effects of NF‐κB p65 subunit knockdown in suppression of nude mouse lung tumour cell xenografts and understands the underlying molecular events. A nude mouse Lewis lung carcinoma cell xenograft model was established, and the mice were intraperitoneally injected with NF‐κB p65 small interfering RNA (siRNA) and sacrificed after 2weeks of tumour cell injection. Tumour xenografts were harvested for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, Western blot and quantitative reverse transcription‐polymerase chain reaction analyses. Results found that compared t...
Source: Cell Biochemistry and Function - July 15, 2015 Category: Biochemistry Authors: Yun Qu, Bo Qu, Xiaofang Wang, Rong Wu, Xiaoye Zhang Tags: Research Article Source Type: research

RU486 Induces Pro‐Apoptotic Endoplasmic Reticulum Stress Through the Induction of CHOP Expression by Enhancing C/EBPδ Expression in Human Renal Carcinoma Caki Cells
This article is protected by copyright. All rights reserved
Source: Journal of Cellular Biochemistry - July 14, 2015 Category: Biochemistry Authors: Ji Hoon Jang, Kyoung‐jin Min, Shin Kim, Jong‐Wook Park, Taeg Kyu Kwon Tags: Article Source Type: research

AP4 activates cell migration and EMT mediated by p53 in MDA-MB-231 breast carcinoma cells.
Abstract Tumor metastasis is the primary cause of mortality in most cancer patients. Before disassociation from the tumors, most of malignant tumor cells undergo the epithelial-mesenchymal transition to break away from the adhesions between the cells and the surrounding extracellular matrix. Recently, activating enhancer-binding protein (AP4) has been shown to be a mediator of EMT in colorectal cancer and high level of AP4 correlates with poor prognosis in cancer patients. It has been found that AP4 upregulates the genes involved in EMT and cell proliferation in colorectal cancer cells and that the aggressive hum...
Source: Molecular and Cellular Biochemistry - June 3, 2015 Category: Biochemistry Authors: Chen S, Chiu SK Tags: Mol Cell Biochem Source Type: research

Deletion of phospholipase A2 group IV c induces apoptosis in rat mammary tumor cells by the nuclear factor-{kappa}B/lipocalin 2 pathway
Although some phospholipase A2 forms, the initiator of the arachidonic acid cascade, contribute to carcinogenesis in many organs, the contribution of phospholipase A2 group IVc (Pla2g4c) remains to be clarified and the function of the enzyme in cancer development are unknown. The Hirosaki hairless rat (HHR), a mutant rat strain with autosomal recessive inheritance, spontaneously derived from the Sprague-Dawley rat (SDR). The HHR showed the low incidence, volume of mammary tumors induced by 7,12-dimethylbenz[a]anthracene were much smaller, and markedly increased TUNEL-positive apoptotic cells were detected. Array comparativ...
Source: BJ Disease - May 27, 2015 Category: Biochemistry Authors: N Nanashima, T Yamada, T Shimizu, S Tsuchida Tags: BJ Signal Source Type: research

Hyper-dependence of breast cancer cell types on the nuclear transporter Importin β1.
Abstract We previously reported that overexpression of members of the Importin (Imp) superfamily of nuclear transporters results in increased nuclear trafficking through conventional transport pathways in tumour cells. Here we show for the first time that the extent of overexpression of Impβ1 correlates with disease state in the MCF10 human breast tumour progression system. Excitingly, we find that targeting Impβ1 activity through siRNA is>30 times more efficient in decreasing the viability of malignant ductal carcinoma cells compared to isogenic non-transformed counterparts, and is highly potent and tumour s...
Source: Biochimica et Biophysica Acta - May 7, 2015 Category: Biochemistry Authors: Kuusisto HV, Jans DA Tags: Biochim Biophys Acta Source Type: research

Elevated GAPDH Expression Is Associated with the Proliferation and Invasion of Lung and Esophageal Squamous Cell Carcinomas
This article is protected by copyright. All rights reserved
Source: Proteomics - May 6, 2015 Category: Biochemistry Authors: Lihong Hao, Xin Zhou, Shuqing Liu, Mingzhong Sun, Yang Song, Sha Du, Bing Sun, Chunmei Guo, Linlin Gong, Jun Hu, Hongwei Guan, Shujuan Shao Tags: Research Article Source Type: research

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