Optimization of extraction, antioxidant activity and functional properties of quince seed mucilage by RSM.
Abstract
The effect of extraction temperature (25-65°C), extraction temperature (5-25min) and water to seed ratio (10:1-50:1) on yield, protein content, turbidity, viscosity, antioxidant activity, emulsion stability and foam stability of quince seed mucilage was investigated using response surface methodology (RSM). Experiments were designed according to Central Composite Face Center Design with these seven factors, including central and axial points. Numerical optimization determined the optimum extraction conditions based on the highest yield, viscosity, antioxidant activity, emulsion stability and foam...
Source: International Journal of Biological Macromolecules - February 17, 2014 Category: Biochemistry Authors: Jouki M, Mortazavi SA, Yazdi FT, Koocheki A Tags: Int J Biol Macromol Source Type: research
RNA isolation from loquat and other recalcitrant woody plants with high quality and yield.
Abstract
RNA isolation is difficult in plants that contain large amounts of polysaccharides and polyphenol compounds. To date, no commercial kit has been developed for the isolation of high-quality RNA from tissues with these characteristics, especially for fruit. The common protocols for RNA isolation are tedious and usually result in poor yields when applied to recalcitrant plant tissues. Here an efficient RNA isolation protocol based on cetyltrimethylammonium bromide (CTAB) and two successive precipitations with 10 M lithium chloride (LiCl) was developed specifically for loquat fruits but it was proved ...
Source: Analytical Biochemistry - February 17, 2014 Category: Biochemistry Authors: Morante-Carriel J, Sellés-Marchart S, Martínez-Márquez A, Martínez-Esteso MJ, Luque I, Bru-Martínez R Tags: Anal Biochem Source Type: research
Changes in Plasma Phenolic Metabolites of Rats Administered Different Molecular‐Weight Polyphenol Fractions from Chinese Quince Fruit Extracts
Abstract
Chinese quince is a medicinal fruit rich in polyphenols. We investigated the effects of oral administration of Chinese quince polyphenols extracted with boiling water on plasma phenolic metabolites in rats. Plasma vanillic acid concentration increased for 6 h after administration of both low molecular‐weight polyphenolic fraction (LMW‐F) and high molecular‐weight polyphenolic fraction (HMW‐F). The increase in plasma vanillic acid in the HMW‐F group was 2.1 times greater than that in the LMW‐F group. (–)‐Epicatechin and 3‐hydroxyphenylpropionic acid were detected only in the plasma of the LMW‐...
Source: Journal of Food Biochemistry - February 1, 2014 Category: Food Science Authors: Yasunori Hamauzu, Kazutoshi Nakamura Tags: Full Article Source Type: research
Physical, barrier and antioxidant properties of a novel plasticized edible film from quince seed mucilage.
In this study, we investigated the potential of quince seed mucilage (QSM) as a new source for preparation of edible films and determined the physical, mechanical, barrier, antioxidant, microstructural and thermal properties. QSM films were prepared by incorporation of three levels of glycerol (25-50%, w/w). As glycerol concentration increased, water vapor permeability (WVP), oxygen permeability (O2P), elongation at break (EB), water solubility and moisture content of QSM films increased while, tensile strength (TS), density and surface hydrophobicity decreased significantly. The measurement of color values showed that by ...
Source: International Journal of Biological Macromolecules - September 26, 2013 Category: Biochemistry Authors: Jouki M, Tabatabaei Yazdi F, Mortazavi SA, Koocheki A Tags: Int J Biol Macromol Source Type: research
Drug release from nanoparticles embedded in four different nanofibrillar cellulose aerogels.
Abstract
Highly porous nanocellulose aerogels prepared by freeze-drying from various nanofibrillar cellulose (NFC) hydrogels are introduced as nanoparticle reservoirs for oral drug delivery systems. Here we show that beclomethasone dipropionate (BDP) nanoparticles coated with amphiphilic hydrophobin proteins can be well integrated into the NFC aerogels. NFCs from four different origins are introduced and compared to microcrystalline cellulose (MCC). The nanocellulose aerogel scaffolds made from red pepper (RC) and MCC release the drug immediately, while bacterial cellulose (BC), quince seed (QC) and TEMPO-...
Source: European Journal of Pharmaceutical Sciences - March 14, 2013 Category: Drugs & Pharmacology Authors: Valo H, Arola S, Laaksonen P, Torkkeli M, Peltonen L, Linder MB, Serimaa R, Kuga S, Hirvonen J, Laaksonen T Tags: Eur J Pharm Sci Source Type: research
Flavanols from Japanese quince (chaenomeles japonica) fruit inhibit human prostate and breast cancer cell line invasiveness and cause favorable changes in bax/bcl-2 mRNA ratio.
In conclusion, JQFFP exhibited a potent antiproliferative effect against cancer cells, inhibited their invasiveness, and decreased expression level of several genes involved in apoptosis, angiogenesis, and metastasis.
PMID: 23441615 [PubMed - in process] (Source: Nutrition and Cancer)
Source: Nutrition and Cancer - February 1, 2013 Category: Cancer & Oncology Authors: Lewandowska U, Szewczyk K, Owczarek K, Hrabec Z, Podsędek A, Koziołkiewicz M, Hrabec E Tags: Nutr Cancer Source Type: research
