Utility of VerifyNow to assess the immediate pharmacodynamic response of chewed and swallowed aspirin: comparison with aggregometry
Platelets. 2024 Dec;35(1):2298352. doi: 10.1080/09537104.2023.2298352. Epub 2024 Jan 3.ABSTRACTVerifyNow (VN) test is a less laborious method to assess pharmacodynamics (PD) compared to light transmittance aggregometry (LTA). VN assay has not been used to study the immediate PD effects of acetylsalicylic acid (ASA). Ten healthy volunteers were randomly assigned to a single 162 or 650 mg dose of chewed and swallowed ASA. Pharmacodynamic and pharmacokinetic measurements were performed at baseline and serially up to 60 min after ASA administration. Onset by VN was 20 ± 7 min with 162 mg and 13 ± 7 min with 650 mg ASA (p = ....
Source: Platelets - January 3, 2024 Category: Hematology Authors: Kevin P Bliden Parshotam Kundan Danielle Kraft Rueshil Parekh Sahib Singh Aravind D Babu Anika P Shah Udaya S Tantry Paul A Gurbel Source Type: research
The rapid change of shear rate gradient is beneficial to platelet activation
In this study, we developed a set of microfluidic chips capable of generating different shear gradients and simulated the shear rate distribution in the flow field by COMSOL Multiphysics software. Molecular markers of platelet activation (P-selectin, activated GPIIb/IIIa, phosphatidylserine exposure, and monocyte-platelet aggregate formation) were analyzed by flow cytometry. Platelet aggregation induced by shear gradient was studied by a microfluidic experimental platform, and plasma vWF ristocetin cofactor (vWF: RCO) activity was investigated by flow cytometry. The expression of p-Akt was studied by Western blotting. The ...
Source: Platelets - December 15, 2023 Category: Hematology Authors: Tiancong Zhang Ling Liu Xiaojing Huang Xuemei Gao Xuanrong Huan Cui He Yuan Li Source Type: research
A case-control study of bleeding risk in children with 22q11.2 deletion syndrome undergoing cardiac surgery
This study sought to identify specific risk factors for bleeding in this patient population. Examine the factors determining bleeding and transfusion requirements in patients with 22q11.2DS undergoing cardiac surgery. This was a single center review of patients who underwent cardiac surgery at the Children's Hospital of Philadelphia from 2000 to 2016. Data was extracted from the medical record. Frequency of bleeding events, laboratory values, and transfusion requirements were compared. We included 226 patients with 22q11.2DS and 506 controls. Bleeding events were identified in 13 patients with 22q11.2DS (5.8%) and 27 contr...
Source: Platelets - December 15, 2023 Category: Hematology Authors: T Blaine Crowley Ian Campbell Abinaya Arulselvan David Friedman Elaine H Zackai Tracy R Geoffrion Char Witmer J William Gaynor Donna M McDonald-McGinn Michele P Lambert Source Type: research
Role of platelet count in a murine stasis model of deep vein thrombosis
We examined the role of thrombopoietin-altered platelet count on thrombus burden in a murine stasis model of DVT. To modulate platelet count compared to baseline, CD1 mice were pretreated with thrombopoietin antisense oligonucleotide (THPO-ASO, 56% decrease), thrombopoietin mimetic (TPO-mimetic, 36% increase), or saline (within 1%). Thrombi and vein walls were examined on postoperative days (POD) 3 and 7. Thrombus weights on POD 3 were not different between treatment groups (p = .84). The mean thrombus weights on POD 7 were significantly increased in the TPO-mimetic cohort compared to the THPO-ASO (p = .005) and the saline...
Source: Platelets - December 15, 2023 Category: Hematology Authors: Rick Mathews Naly Setthavongsack Anh Le-Cook Andy Kaempf Jennifer M Loftis Randall L Woltjer Christina U Lorentz Alexey Revenko Monica T Hinds Khanh P Nguyen Source Type: research
The rapid change of shear rate gradient is beneficial to platelet activation
In this study, we developed a set of microfluidic chips capable of generating different shear gradients and simulated the shear rate distribution in the flow field by COMSOL Multiphysics software. Molecular markers of platelet activation (P-selectin, activated GPIIb/IIIa, phosphatidylserine exposure, and monocyte-platelet aggregate formation) were analyzed by flow cytometry. Platelet aggregation induced by shear gradient was studied by a microfluidic experimental platform, and plasma vWF ristocetin cofactor (vWF: RCO) activity was investigated by flow cytometry. The expression of p-Akt was studied by Western blotting. The ...
Source: Platelets - December 15, 2023 Category: Hematology Authors: Tiancong Zhang Ling Liu Xiaojing Huang Xuemei Gao Xuanrong Huan Cui He Yuan Li Source Type: research
A case-control study of bleeding risk in children with 22q11.2 deletion syndrome undergoing cardiac surgery
This study sought to identify specific risk factors for bleeding in this patient population. Examine the factors determining bleeding and transfusion requirements in patients with 22q11.2DS undergoing cardiac surgery. This was a single center review of patients who underwent cardiac surgery at the Children's Hospital of Philadelphia from 2000 to 2016. Data was extracted from the medical record. Frequency of bleeding events, laboratory values, and transfusion requirements were compared. We included 226 patients with 22q11.2DS and 506 controls. Bleeding events were identified in 13 patients with 22q11.2DS (5.8%) and 27 contr...
Source: Platelets - December 15, 2023 Category: Hematology Authors: T Blaine Crowley Ian Campbell Abinaya Arulselvan David Friedman Elaine H Zackai Tracy R Geoffrion Char Witmer J William Gaynor Donna M McDonald-McGinn Michele P Lambert Source Type: research
Role of platelet count in a murine stasis model of deep vein thrombosis
We examined the role of thrombopoietin-altered platelet count on thrombus burden in a murine stasis model of DVT. To modulate platelet count compared to baseline, CD1 mice were pretreated with thrombopoietin antisense oligonucleotide (THPO-ASO, 56% decrease), thrombopoietin mimetic (TPO-mimetic, 36% increase), or saline (within 1%). Thrombi and vein walls were examined on postoperative days (POD) 3 and 7. Thrombus weights on POD 3 were not different between treatment groups (p = .84). The mean thrombus weights on POD 7 were significantly increased in the TPO-mimetic cohort compared to the THPO-ASO (p = .005) and the saline...
Source: Platelets - December 15, 2023 Category: Hematology Authors: Rick Mathews Naly Setthavongsack Anh Le-Cook Andy Kaempf Jennifer M Loftis Randall L Woltjer Christina U Lorentz Alexey Revenko Monica T Hinds Khanh P Nguyen Source Type: research
The rapid change of shear rate gradient is beneficial to platelet activation
In this study, we developed a set of microfluidic chips capable of generating different shear gradients and simulated the shear rate distribution in the flow field by COMSOL Multiphysics software. Molecular markers of platelet activation (P-selectin, activated GPIIb/IIIa, phosphatidylserine exposure, and monocyte-platelet aggregate formation) were analyzed by flow cytometry. Platelet aggregation induced by shear gradient was studied by a microfluidic experimental platform, and plasma vWF ristocetin cofactor (vWF: RCO) activity was investigated by flow cytometry. The expression of p-Akt was studied by Western blotting. The ...
Source: Platelets - December 15, 2023 Category: Hematology Authors: Tiancong Zhang Ling Liu Xiaojing Huang Xuemei Gao Xuanrong Huan Cui He Yuan Li Source Type: research
A case-control study of bleeding risk in children with 22q11.2 deletion syndrome undergoing cardiac surgery
This study sought to identify specific risk factors for bleeding in this patient population. Examine the factors determining bleeding and transfusion requirements in patients with 22q11.2DS undergoing cardiac surgery. This was a single center review of patients who underwent cardiac surgery at the Children's Hospital of Philadelphia from 2000 to 2016. Data was extracted from the medical record. Frequency of bleeding events, laboratory values, and transfusion requirements were compared. We included 226 patients with 22q11.2DS and 506 controls. Bleeding events were identified in 13 patients with 22q11.2DS (5.8%) and 27 contr...
Source: Platelets - December 15, 2023 Category: Hematology Authors: T Blaine Crowley Ian Campbell Abinaya Arulselvan David Friedman Elaine H Zackai Tracy R Geoffrion Char Witmer J William Gaynor Donna M McDonald-McGinn Michele P Lambert Source Type: research
Role of platelet count in a murine stasis model of deep vein thrombosis
We examined the role of thrombopoietin-altered platelet count on thrombus burden in a murine stasis model of DVT. To modulate platelet count compared to baseline, CD1 mice were pretreated with thrombopoietin antisense oligonucleotide (THPO-ASO, 56% decrease), thrombopoietin mimetic (TPO-mimetic, 36% increase), or saline (within 1%). Thrombi and vein walls were examined on postoperative days (POD) 3 and 7. Thrombus weights on POD 3 were not different between treatment groups (p = .84). The mean thrombus weights on POD 7 were significantly increased in the TPO-mimetic cohort compared to the THPO-ASO (p = .005) and the saline...
Source: Platelets - December 15, 2023 Category: Hematology Authors: Rick Mathews Naly Setthavongsack Anh Le-Cook Andy Kaempf Jennifer M Loftis Randall L Woltjer Christina U Lorentz Alexey Revenko Monica T Hinds Khanh P Nguyen Source Type: research
The rapid change of shear rate gradient is beneficial to platelet activation
In this study, we developed a set of microfluidic chips capable of generating different shear gradients and simulated the shear rate distribution in the flow field by COMSOL Multiphysics software. Molecular markers of platelet activation (P-selectin, activated GPIIb/IIIa, phosphatidylserine exposure, and monocyte-platelet aggregate formation) were analyzed by flow cytometry. Platelet aggregation induced by shear gradient was studied by a microfluidic experimental platform, and plasma vWF ristocetin cofactor (vWF: RCO) activity was investigated by flow cytometry. The expression of p-Akt was studied by Western blotting. The ...
Source: Platelets - December 15, 2023 Category: Hematology Authors: Tiancong Zhang Ling Liu Xiaojing Huang Xuemei Gao Xuanrong Huan Cui He Yuan Li Source Type: research
A case-control study of bleeding risk in children with 22q11.2 deletion syndrome undergoing cardiac surgery
This study sought to identify specific risk factors for bleeding in this patient population. Examine the factors determining bleeding and transfusion requirements in patients with 22q11.2DS undergoing cardiac surgery. This was a single center review of patients who underwent cardiac surgery at the Children's Hospital of Philadelphia from 2000 to 2016. Data was extracted from the medical record. Frequency of bleeding events, laboratory values, and transfusion requirements were compared. We included 226 patients with 22q11.2DS and 506 controls. Bleeding events were identified in 13 patients with 22q11.2DS (5.8%) and 27 contr...
Source: Platelets - December 15, 2023 Category: Hematology Authors: T Blaine Crowley Ian Campbell Abinaya Arulselvan David Friedman Elaine H Zackai Tracy R Geoffrion Char Witmer J William Gaynor Donna M McDonald-McGinn Michele P Lambert Source Type: research
