Platelet count and disease - editorial policy.
Authors: Harrison P, Lordkipanidzé M, Frelinger Iii AL, Thomas MR, Watson SP PMID: 32762569 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - August 13, 2020 Category: Hematology Tags: Platelets Source Type: research

Immature cell fractions after cessation of chronic P2Y12-inhibition in patients with coronary artery diseases.
Authors: Jäger B, Vargas KG, Haller PM, Stojkovic S, Kaufmann CC, Freynhofer M, Quehenberger P, Wagner O, Wojta J, Huber K Abstract Changes in circulating cell populations may promote ischemic events that occur soon after discontinuation of P2Y12-inhibition. The aim of the study was to track the course of thrombopoietic and erythropoietic cells in patients with coronary artery diseases (CAD) after planned and physician-driven cessation of chronic P2Y12-inhibition (clopidogrel 75 mg OD, or prasugrel 10 mg OD, or ticagrelor 90 mg BID). Cell fractions were determined in 62 patients at baseline (the last day of P2...
Source: Platelets - August 13, 2020 Category: Hematology Tags: Platelets Source Type: research

Catastrophic antiphospholipid syndrome refractory to high-dose intravenous immunoglobulin responsive to therapeutic plasma exchange.
Authors: James TE, Martin LJ, Warkentin TE, Crowther MA Abstract Catastrophic antiphospholipid syndrome (CAPS) involves sudden multiorgan dysfunction from thrombosis due to antibodies that cause platelet activation and endothelial dysfunction. Treatment variably combines anticoagulation, corticosteroid use, therapeutic plasma exchange (TPE), and high-dose intravenous immunoglobulin (IVIG). A 42-year-old male with antiphospholipid syndrome (APS) presented with severe thrombocytopenia, encephalopathy, cardiac ischemia, and acral purpuric cutaneous lesions. CAPS was identified and he received heparin infusion, methylp...
Source: Platelets - August 13, 2020 Category: Hematology Tags: Platelets Source Type: research

Factor Xa and thrombin induce endothelial progenitor cell activation. The effect of direct oral anticoagulants.
Authors: Papadaki S, Sidiropoulou S, Moschonas IC, Tselepis AD Abstract Factor Xa (FXa) and thrombin exert non-hemostatic cellular actions primarily mediated through protease-activated receptors (PARs). We investigated the effect of FXa and thrombin on human late-outgrowth endothelial cells (OECs), a type of endothelial progenitor cells (EPCs), and on human umbilical vein endothelial cells (HUVECs). The effect of direct oral anticoagulants (DOACs), rivaroxaban and dabigatran, was also studied. The membrane expression of intercellular adhesion molecule-1 (ICAM-1) and the secretion of monocyte chemoattractant protein...
Source: Platelets - August 13, 2020 Category: Hematology Tags: Platelets Source Type: research

Factors Involved in the onset of infection following bacterially contaminated platelet transfusions.
Authors: Wilson-Nieuwenhuis J, El-Mohtadi M, Edwards K, Whitehead K, Dempsey-Hibbert N Abstract Transfusion of platelet concentrates (PCs) is associated with several adverse patient reactions, the most common of which are febrile non-hemolytic transfusion reactions (FNHTRs) and transfusion-associated bacterial-infection/transfusion-associated sepsis (T-ABI/TA-S). Diagnosis of T-ABI/T-AS requires a positive blood culture (BC) result from the transfusion recipient and also a positive identification of bacterial contamination within a test aliquot of the transfused PC. In a significant number of cases, clinical sympto...
Source: Platelets - August 13, 2020 Category: Hematology Tags: Platelets Source Type: research

Isolation of murine bone marrow by centrifugation or flushing for the analysis of hematopoietic cells - a comparative study.
Authors: Heib T, Gross C, Müller ML, Stegner D, Pleines I Abstract Investigation of the bone marrow as the main compartment of hematopoiesis is critical in many research fields. Here, we adapted a centrifugation-based method for the isolation of murine bone marrow and compared it to the traditional flushing method. Analysis of primary hematopoietic stem cells, immune cells, and megakaryocytes revealed a comparable distribution of cellular (sub)populations. Furthermore, in vitro differentiated megakaryocytes displayed unaltered proplatelet formation. Strikingly, bone marrow isolation by centrifugation was consi...
Source: Platelets - July 31, 2020 Category: Hematology Tags: Platelets Source Type: research

Editorial.
Authors: Ghevaert C PMID: 32697612 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - July 24, 2020 Category: Hematology Tags: Platelets Source Type: research

Effect of differently coated silver nanoparticles on hemostasis.
This study aimed to evaluate comprehensively AgNPs effects on hemostasis in human blood by exploiting combination of affordable and clinically relevant techniques. Differently stabilized AgNPs were prepared using sodium bis(2-ethylhexyl)sulphosuccinate (AOT), polyvinylpyrrolidone (PVP), poly-L-lysine (PLL), and bovine serum albumin (BSA) as coating agents. They were tested for hemolytic activity, induction of platelet aggregation, plasmatic coagulation, thrombin generation, and hemostasis in whole blood. All AgNPs were found to cause dose-dependent hemolysis. The BSA-, AOT-, and PVP-coated AgNPs delayed plasmatic coagulati...
Source: Platelets - July 18, 2020 Category: Hematology Tags: Platelets Source Type: research

Aspirin after PCI: in the twilight of its years?
Authors: Parker WAE PMID: 32669035 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - July 18, 2020 Category: Hematology Tags: Platelets Source Type: research

Potential contrasting effects of platelets on the migration and invasion of sarcomas versus carcinomas.
This study was undertaken to simultaneously compare the effects of platelets on murine and human sarcomas and carcinomas. In contrast to their effect on carcinomas, platelets inhibited the invasion of some murine- and all human sarcomas tested in vitro. Further invasion studies with TGFβ treatment only partially recapitulated the results seen with whole platelets. In a spontaneous tumor growth and lung metastasis model, platelets promoted 4T1 mammary carcinoma metastasis but not MCA-1 fibrosarcoma metastasis. Gene expression analysis of the platelet-promoted MDA-MB-231 breast carcinoma, and the platelet-inhibited HT10...
Source: Platelets - July 17, 2020 Category: Hematology Tags: Platelets Source Type: research

Risk of major adverse cardiovascular events of CYP2C19 loss-of-function genotype guided prasugrel/ticagrelor vs clopidogrel therapy for acute coronary syndrome patients undergoing percutaneous coronary intervention: a meta-analysis.
Authors: Biswas M, Kali MSK, Biswas TK, Ibrahim B Abstract The most effective antiplatelet treatments for acute coronary syndrome (ACS) patients carrying CYP2C19 loss-of-function (LoF) alleles undergoing percutaneous coronary intervention (PCI) is still debating and conflicting. It was aimed to compare the efficacy and safety endpoints for these patients treated with alternative P2Y12 receptor blockers (e.g. prasugrel or ticagrelor) against clopidogrel. Literature was searched in PubMed, Cochrane library, Synapse and 1000 Genomes databases following PRISMA guidelines for identifying relevant studies. Aggregated ris...
Source: Platelets - July 17, 2020 Category: Hematology Tags: Platelets Source Type: research

Statins potentiate the antibacterial effect of platelets on Staphylococcus aureus.
Authors: Hannachi N, Fournier PE, Martel H, Habib G, Camoin-Jau L Abstract Platelets have largely demonstrated their implication in anti-infectious immunity. This effect is ensured by the secreted molecules stored mostly in platelet alpha granules. Previous studies have reported that Staphylococcus aureus showed sensitivity to this antibacterial effect of platelets. Statins, for their part, have shown a modulating effect on platelet activation. Furthermore, several studies have reported a protective effect of statins in staphylococcal endocarditis. The aim of this study was to investigate the influence of statins o...
Source: Platelets - July 17, 2020 Category: Hematology Tags: Platelets Source Type: research

Diagnostic challenges in von Willebrand disease. Report of two cases with emphasis on multimeric and molecular analysis.
We report two cases of infrequent inherited variants of VWD with unclear preliminary results with the test panel available at the time of first consultation and that were finally diagnosed as a VWD type 2A/IID with a c.8318 G > C, p.Cys2773Ser mutation and a VWD type 2M with c.4225 T > G, p.Val1409Phe mutation, respectively. The description of these two cases highlights that despite the limited diagnostic panel for the evaluation of von Willebrand Factor (VWF) functionality, the multimeric analysis and genetic family studies were fundamental tools to achieve the final diagnosis. PMID...
Source: Platelets - July 17, 2020 Category: Hematology Tags: Platelets Source Type: research

Relation between mutations in the 5' UTR of ANKRD26 gene and inherited thrombocytopenia with predisposition to myeloid malignancies. An Egyptian study.
Authors: Zidan NI, AbdElmonem DM, Elsheikh HM, Metwally EA, Mokhtar WA, Osman GM Abstract Inherited thrombocytopenias are a heterogeneous group of diseases characterized by a reduced number of platelets and a bleeding tendency that ranges from very mild to life threatening especially in surgery. Mutations in the 5' untranslated region (UTR) of Ankirin repeat domain 26 (ANKRD26) are responsible for autosomal-dominant form of thrombocytopenia, that is known as ANKRD26-related thrombocytopenia (ANKRD26 RT), characterized by a moderate thrombocytopenia with mild propensity to bleeding and predisposition to hematologica...
Source: Platelets - July 15, 2020 Category: Hematology Tags: Platelets Source Type: research

Efficacy and safety of short-term 1-3 months versus standard 12 months dual antiplatelet therapy in patients undergoing percutaneous coronary intervention: a meta-analysis of randomized clinical trials.
In conclusion, the short-term DAPT can reduce the risk of bleeding compared with standard DAPT, without increasing the risk of death or ischemia (Registered by PROSPERO, CRD42020153881). PMID: 32627616 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - July 8, 2020 Category: Hematology Tags: Platelets Source Type: research

Absence of bleeding upon dual antiplatelet therapy in a patient with a immune GPVI deficiency.
We report the case of a patient presenting an acquired GPVI deficiency different from those previously described: (i) he is male whereas all previous cases were female, (ii) he is heterozygous for a mutation in α (alpha)-actinin-1 gene and (iii) he was treated with dual antiplatelet therapy with no haemorrhagic manifestation. PMID: 32627625 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - July 8, 2020 Category: Hematology Tags: Platelets Source Type: research

Platelet count and function in umbilical cord blood versus peripheral blood in term neonates.
Authors: Grevsen AK, Hviid CVB, Hansen AK, Hvas AM Abstract Platelet function in neonates is sparsely investigated. The majority of previous studies investigated platelets in umbilical cord (UC) blood rather than in peripheral blood. We included 20 term neonates and sampled UC blood and peripheral blood within 20 min and 24 h after birth. Platelet count and mean platelet volume (MPV) were measured. Platelet surface glycoproteins (GP) and platelet activation (bound fibrinogen, CD63 and p-selectin) after agonist stimulation were examined by flow cytometry. Platelet aggregation was evaluated by impedance agg...
Source: Platelets - July 8, 2020 Category: Hematology Tags: Platelets Source Type: research

A novel homozygous GFI1B variant in 2 sisters with thrombocytopenia and severe bleeding tendency.
We report a novel homozygous GFI1B (Thr174Ile) variant located in the first Zinc finger domain of GFI1B in two sisters of Palestinian ancestry born to consanguineous parents. They experienced severe bleeding tendency at moderately reduced platelet counts. Flow cytometry and immunofluorescent microscopy confirmed the diagnostic features of GFI1B associated IT: a reduced content of alpha granules and aberrant expression of the stem cell marker CD34 on platelets. Transcription factor GFI1B is differentially expressed during hemato- and lymphopoiesis. In addition, to platelet function investigations, we present results of lymp...
Source: Platelets - July 8, 2020 Category: Hematology Tags: Platelets Source Type: research

Expression of ADP receptor P2Y12, thromboxane A2 receptor and C-type lectin-like receptor 2 in cord blood-derived megakaryopoiesis.
Authors: Gerhards C, Uhlig S, Etemad M, Christodoulou F, Bieback K, Klüter H, Bugert P Abstract The ADP receptor P2Y12, the thromboxane A2 receptor (TXA2R) and the C-type lectin-like receptor 2 (CLEC-2) mediate platelet activation by different mechanisms. Only little is known about the expression of the receptors in human megakaryopoiesis. Our study aimed to establish a flow cytometry (FC) method for the measurement of P2Y12, TXA2R, and CLEC-2 on platelets of healthy donors and to monitor receptor expression in ex vivo megakaryopoiesis. We determined mean fluorescence intensity (MFI) values of FITC, PE, or APC...
Source: Platelets - July 5, 2020 Category: Hematology Tags: Platelets Source Type: research

Novel antiplatelet strategies targeting VWF and GPIb.
Authors: Prasannan N, Scully M Abstract Von Willebrand factor has a pivotal role in primary hemostasis. Its role in thrombotic microangiopathies (TMA), as well as cardiovascular disease, has been demonstrated. Thrombotic thrombocytopenic purpura (TTP), a thrombotic microangiopathy, is a life-threatening condition with a high mortality rate if untreated. Current management strategies comprise plasma exchange to remove autoantibodies and replenish ADAMTS13, along with immunosuppressive agents in immune TTP. This review focuses on novel antiplatelet strategies that target VWF and GPIb. The benefits of the nanobody cap...
Source: Platelets - July 4, 2020 Category: Hematology Tags: Platelets Source Type: research

Risk stratification and outcomes of intracranial hemorrhage in patients with immune thrombocytopenia under 60 years of age.
Authors: Zhao P, Hou M, Liu Y, Liu HX, Huang RB, Yao HX, Niu T, Peng J, Jiang M, Han YQ, Hu JD, Zhou H, Zhou ZP, Qiu L, Zhang LS, Wang X, Wang HQ, Feng R, Yang LH, Ma LM, Wang SQ, Kong PY, Wang WS, Sun HP, Sun J, Zhou HB, Zhu TN, Wang LR, Zhang JY, Huang QS, Liu X, Fu HX, Li YY, Wang QF, Jiang Q, Jiang H, Lu J, Zhang XH Abstract Intracranial hemorrhage (ICH) is a devastating complication of immune thrombocytopenia (ITP). However, information on ICH in ITP patients under the age of 60 years is limited, and no predictive tools are available in clinical practice. A total of 93 adult patients with ITP who develope...
Source: Platelets - July 4, 2020 Category: Hematology Tags: Platelets Source Type: research

Role of platelets and megakaryocytes in adaptive immunity.
Authors: Marcoux G, Laroche A, Espinoza Romero J, Boilard E Abstract The immune system is comprised of two principal interconnected components called innate and adaptive immunity. While the innate immune system mounts a nonspecific response that provides protection against the spread of foreign pathogens, the adaptive immune system has developed to specifically recognize a given pathogen and lead to immunological memory. Platelets are small fragments produced from megakaryocytes in bone marrow and lungs. They circulate throughout the blood to monitor the integrity of the vasculature and to prevent bleeding. Given t...
Source: Platelets - July 2, 2020 Category: Hematology Tags: Platelets Source Type: research

A novel missense variant in the RASGRP2 gene in patients with moderate to severe bleeding disorder.
Authors: Alharby E, Bakhsh MA, Albalawi AM, Almutairi SO, Hashmi JA, Basit S Abstract Inherited platelet function disorder-18 (IPD-18) is a relatively new non-syndromic autosomal recessive bleeding disorder. It is characterized by deficient or dysfunctional CalDAG-GEFI protein. The distinctive feature of the disease is impaired platelet aggregation in response to multiple physiologic agonists. We here report a family with a platelet-type bleeding disorder and a novel mutation in the RASGRP2 gene. The overall bleeding score for the affected individuals was 15 and 12. Based on the initial diagnosis of Glanzmann throm...
Source: Platelets - July 2, 2020 Category: Hematology Tags: Platelets Source Type: research

PTGS1 gene variations associated with bleeding and platelet dysfunction.
Authors: Palma-Barqueros V, Bohdan N, Revilla N, Vicente V, Bastida JM, Rivera J PMID: 32584621 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - June 27, 2020 Category: Hematology Tags: Platelets Source Type: research

Platelet removal by single-step centrifugation.
The objective of this study is to develop an easy, fast and clinically applicable centrifugation protocol to produce essentially platelet-free plasma with a high yield for EV research. We compared the two-step centrifugation protocol to a single-step protocol at 5,000 g for 20 minutes. The removal of platelets was computationally predicted and experimentally validated. Flow cytometry was used to detect residual platelets and platelet-derived (CD61+) EVs. The single-step protocol at 5,000 g (i) is less laborious and approximately ten minutes faster, (ii) removes platelets as effective as the two-step centrifu...
Source: Platelets - June 21, 2020 Category: Hematology Tags: Platelets Source Type: research

A rationale for blocking thromboinflammation in COVID-19 with Btk inhibitors.
Authors: Nicolson PL, Welsh JD, Chauhan A, Thomas MR, Kahn ML, Watson SP PMID: 32552307 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - June 21, 2020 Category: Hematology Tags: Platelets Source Type: research

Prolonged enoxaparin therapy compared with standard-of-care antithrombotic therapy in opiate-treated patients undergoing primary percutaneous coronary intervention.
Authors: Sumaya W, Parker WAE, Judge HM, Hall IR, Orme RC, Adam Z, Richardson JD, Rothman AMK, Morgan KP, Gunn JP, Storey RF Abstract A novel enoxaparin regimen consisting of intra-arterial bolus (0.75 mg/kg) followed by intravenous infusion (0.75 mg/kg/6 hours) has been developed as a possible solution to the delayed absorption of oral P2Y12 inhibitors in opiate-treated ST-elevation myocardial infarction (STEMI) patients undergoing primary angioplasty. We aimed to study the feasibility of this regimen as an alternative to standard-of-care treatment (SOC) with unfractionated heparin ± glycoprotein IIb/I...
Source: Platelets - June 18, 2020 Category: Hematology Tags: Platelets Source Type: research

The efficacy and safety of CYP2C19 genotype-guided antiplatelet therapy compared with conventional antiplatelet therapy in patients with acute coronary syndrome or undergoing percutaneous coronary intervention: A meta-analysis of randomized controlled trials.
This study aimed to evaluate the efficacy and safety of CYP2C19 genotype-guided antiplatelet therapy in patients with ACS or undergoing PCI. PubMed, EMBASE, the Cochrane Library and clinicaltrials.gov were searched to identify randomized controlled trials (RCTs) comparing CYP2C19 genotype-guided antiplatelet therapy with conventional therapy in patients with ACS or undergoing PCI. Eight RCTs involving 6708 patients were included in this meta-analysis. CYP2C19 genotype-guided antiplatelet therapy was slightly superior to the conventional antiplatelet therapy in reducing the risk of MACE [RR(95%CI): 0.71(0.51-0.98), p = ...
Source: Platelets - June 18, 2020 Category: Hematology Tags: Platelets Source Type: research

ABCG5 and ABCG8 gene variations associated with sitosterolemia and platelet dysfunction.
Authors: Bastida JM, Benito R, González-Porras JR, Rivera J PMID: 32546081 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - June 18, 2020 Category: Hematology Tags: Platelets Source Type: research

MicroRNA-126 is a regulator of platelet-supported thrombin generation.
Authors: Zapilko V, Fish RJ, Garcia A, Reny JL, Dunoyer-Geindre S, Lecompte T, Neerman-Arbez M, Fontana P Abstract Circulating microRNA (miRNA) expression profiles correlate with platelet reactivity. MiR-126 is a promising candidates in this regard. We generated a transgenic zebrafish line with thrombocyte-specific overexpression of miR-126. Laser injury of the posterior cardinal vein of 5 day-old larvae was performed with or without antithrombotic pre-treatment. Platelet-like structures (PLS) derived from human megakaryocytes transfected with miR-126 were also evaluated for procoagulant activity. Finally, we studi...
Source: Platelets - June 14, 2020 Category: Hematology Tags: Platelets Source Type: research

Novel antiplatelet targets in the treatment of acute coronary syndromes.
Authors: Alenazy FO, Thomas MR Abstract Acute coronary syndromes (ACS) are a global cause of mortality and morbidity that affect millions of lives worldwide. Following atherosclerotic plaque rupture, platelet activation and aggregation are the two major elements that initiate thrombus formation inside a coronary artery, which can obstruct blood flow and cause myocardial ischemia; ergo, antiplatelet therapy forms a major part of the treatment strategy for ACS. Patients with ACS routinely receive dual antiplatelet therapy (DAPT), which consists of aspirin and a platelet P2Y12 inhibitor to both treat and prevent ather...
Source: Platelets - June 14, 2020 Category: Hematology Tags: Platelets Source Type: research

In vivo evaluation of the biocompatibility and biodegradation of a new denatured plasma membrane combined with liquid PRF (Alb-PRF).
This study demonstrates-for the first time, to the best of our knowledge-a marked improvement in the membrane stability of Alb-PRF. This indicates its future potential for use as a biological barrier membrane for GBR procedures with a long-lasting half-life, or as a biological filler material in esthetic medicine applications. Thus, further studies are warranted to explore future clinical applications in various fields of medicine. PMID: 32531175 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - June 14, 2020 Category: Hematology Tags: Platelets Source Type: research

Mechanisms of platelet release: in vivo studies and in vitro modeling.
Authors: Abbonante V, Di Buduo CA, Malara A, Laurent PA, Balduini A Abstract Mechanisms related to platelet release in the context of the bone marrow niche are not completely known. In this review we discuss what has been discovered about four critical aspects of this process: 1) the bone marrow niche organization, 2) the role of the extracellular matrix components, 3) the mechanisms by which megakaryocytes release platelets and 4) the novel approaches to mimic the bone marrow environment and produce platelets ex vivo. PMID: 32522064 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - June 12, 2020 Category: Hematology Tags: Platelets Source Type: research

Harmonizing light transmission aggregometry in the Netherlands by implementation of the SSC-ISTH guideline.
Authors: Munnix ICA, Van Oerle R, Verhezen P, Kuijper P, Hackeng CM, Hopman-Kerkhoff HIJ, Hudig F, Van De Kerkhof D, Leyte A, De Maat MPM, Oude Elferink RFM, Ruinemans-Koerts J, Schoorl M, Slomp J, Soons H, Stroobants A, Van Wijk E, Henskens YMC Abstract Light transmission aggregometry (LTA) is considered the gold standard method for evaluation of platelet function. However, there are a lot of variation in protocols (pre-analytical procedures and agonist concentrations) and results. The aim of our study was to establish a national LTA protocol, to investigate the effect of standardization and to define national ref...
Source: Platelets - June 12, 2020 Category: Hematology Tags: Platelets Source Type: research

Benefit-risk profile of extended dual antiplatelet therapy beyond 1 year in patients with high risk of ischemic or bleeding events after PCI.
Authors: Wang HY, Gao RL, Xu B, Yang YJ, Yin D, Wang Y, Dou KF Abstract The benefits and harms of dual antiplatelet therapy (DAPT) continuation with aspirin and clopidogrel beyond 1 year after percutaneous coronary intervention (PCI) with drug-eluting stent (DES) implantation for high ischemic or bleeding risk patients remain unclear. All consecutive patients undergoing PCI were prospectively included in the Fuwai PCI Registry from January 2013 to December 2013. We evaluated 7521 patients who were at high risk for thrombotic or hemorrhagic complications and were events free at 1 year after the index procedure. &quo...
Source: Platelets - June 7, 2020 Category: Hematology Tags: Platelets Source Type: research

Platelet count and risk of major bleeding in venous thromboembolism.
Authors: Johnsen HS, Braekkan SK, Morelli VM, Hansen JB Abstract The relationship between platelet count and risk of major bleeding in patients with venous thromboembolism (VTE) during anticoagulation remains unclear. We therefore investigated the association between platelet count, measured at VTE diagnosis and before the thrombotic event, and risk of major bleeding. Participants comprised 744 patients with incident VTE derived from the Tromsø Study. Major bleedings were recorded during the first year after VTE. Cox-regression was used to calculate hazard ratios (HRs) for major bleeding across platelet coun...
Source: Platelets - June 7, 2020 Category: Hematology Tags: Platelets Source Type: research

New insights into glycoprotein Ib α desialylation-mediated platelet clearance.
New insights into glycoprotein Ibα desialylation-mediated platelet clearance. Platelets. 2020 Jun 04;:1-3 Authors: Yule J, Morgan NV, Poulter NS PMID: 32498669 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - June 7, 2020 Category: Hematology Tags: Platelets Source Type: research

Inflammatory state does not affect the antiplatelet efficacy of potent P2Y12 inhibitors in ACS.
In conclusion, inflammatory state does not affect the antiplatelet efficacy of potent P2Y12 inhibitors in the acute phase of ACS, confirming the safety and efficacy of potent P2Y12 inhibitors in patients with a high inflammatory burden. PMID: 32501749 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - June 7, 2020 Category: Hematology Tags: Platelets Source Type: research

In vitro-derived platelets: the challenges we will have to face to assess quality and safety.
Authors: Mookerjee S, Foster HR, Waller AK, Ghevaert CJ Abstract Platelet transfusions are given to patients in hospital who have a low blood platelet count (thrombocytopenia) either because of major bleeding (following trauma or surgery) or because the bone marrow production of platelets is impaired often due to chemotherapy, infiltration with malignant cells, fibrosis or genetic disorders. We are currently entirely reliant on blood donors as a source of platelets in transfusion medicine. However, the demand for platelets continues to rise, driven by an aging population, advances in medical procedures and ever mor...
Source: Platelets - June 5, 2020 Category: Hematology Tags: Platelets Source Type: research

Sex differences in platelet reactivity in patients with myocardial infarction treated with triple antiplatelet therapy - results from assessing platelet activity in coronary heart disease (APACHE).
Authors: Holm A, Swahn E, Lawesson SS, Gustafsson KM, Janzon M, Jonasson L, Lindahl TL, Alfredsson J Abstract )Several earlier studies have reported increased risk of bleeding in women with myocardial infarction, (MI) compared to men. The reasons for the observed difference are incompletely understood, but one suggested explanation has been excess dosing of antithrombotic drugs in women. The aim of this prospective observational study was to assess sex differences in platelet activity in patients treated with three different platelet inhibitors. We recruited 125 patients (37 women and 88 men) with MI, scheduled for...
Source: Platelets - June 5, 2020 Category: Hematology Tags: Platelets Source Type: research

Hemostatic laboratory derangements in COVID-19 with a focus on platelet count.
Authors: Amgalan A, Othman M Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the coronavirus disease in 2019 (COVID-19) which rapidly evolved from an outbreak in Wuhan, China into a pandemic that has resulted in over millions of infections and over hundreds of thousands of mortalities worldwide. Various coagulopathies have been reported in association with COVID-19, including disseminated intravascular coagulation (DIC), sepsis-induced coagulopathy (SIC), local microthrombi, venous thromboembolism (VTE), arterial thrombotic complications, and thrombo-inflammation. There is a...
Source: Platelets - May 29, 2020 Category: Hematology Tags: Platelets Source Type: research

Effect of advanced glycation end products on platelet activation and aggregation: a comparative study of the role of glyoxal and methylglyoxal.
Authors: Arriagada-Petersen C, Fernandez P, Gomez M, Ravello N, Palomo I, Fuentes E, Ávila F Abstract Advanced glycation end products (AGEs) arising from dietary intake have been associated with numerous chronic diseases including cardiovascular diseases. The interaction between platelets and AGEs has been proposed to play a role in the etiology of cardiovascular diseases. However, the effects of the interaction between platelets and Maillard reaction products generated from glyoxal (Gly) or methylglyoxal (MG) are poorly understood. In this work, the effects of AGEs generated by the reaction between Gly or M...
Source: Platelets - May 26, 2020 Category: Hematology Tags: Platelets Source Type: research

A handshake between platelets and neutrophils might fuel deep vein thrombosis.
Authors: Kenny M, Schoen I PMID: 32449631 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - May 26, 2020 Category: Hematology Tags: Platelets Source Type: research

Genetic variants associated with Hermansky-Pudlak syndrome.
Authors: Merideth MA, Introne WJ, Wang JA, O'Brien KJ, Huizing M, Gochuico BR Abstract Hermansky-Pudlak syndrome (HPS) is a rare autosomal recessive disorder characterized by defective biogenesis of lysosome-related organelles. Clinical manifestations include a bleeding diathesis due to a platelet delta storage pool deficiency, oculocutaneous albinism, inflammatory bowel disease, neutropenia, and pulmonary fibrosis. Ten genes associated with HPS are identified to date, and each gene encodes a protein subunit of either Biogenesis of Lysosome-related Organelles Complex (BLOC)-1, BLOC-2, BLOC-3, or the Adaptor Protein...
Source: Platelets - May 23, 2020 Category: Hematology Tags: Platelets Source Type: research

Platelet integrin activation surfs the calcium waves.
Authors: Ngo ATP, Jongen M, Shatzel JJ, McCarty OJT PMID: 32441552 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - May 23, 2020 Category: Hematology Tags: Platelets Source Type: research

Use of electron microscopy to study platelets and thrombi.
Authors: Tomaiuolo M, Litvinov RI, Weisel JW, Stalker TJ Abstract Electron microscopy has been a valuable tool for the study of platelet biology and thrombosis for more than 70 years. Early studies using conventional transmission and scanning electron microscopy (EM) provided a foundation for our initial understanding of platelet structure and how it changes upon platelet activation. EM approaches have since been utilized to study platelets and thrombi in the context of basic, translational and clinical research, and they are instrumental in the diagnosis of multiple platelet function disorders. In this brief ...
Source: Platelets - May 21, 2020 Category: Hematology Tags: Platelets Source Type: research

Seeing is believing: use of advanced imaging to study platelets and megakaryocytes.
Authors: Thomas SG, Poulter NS PMID: 32427510 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - May 21, 2020 Category: Hematology Tags: Platelets Source Type: research

Highly impaired platelet ultrastructure in two families with novel IKZF5 variants.
Authors: Leinoe E, Kjaersgaard M, Zetterberg E, Ostrowski S, Greinacher A, Rossing M Abstract Heterozygous variants in the IKZF5 gene, encoding transcription factor Pegasus, were recently discovered to be causal of inherited thrombocytopenia (IT). We screened 90 patients suspected of inherited thrombocytopenia for variants in 101 genes associated with inherited bleeding disorders and report the clinical presentation of two Danish families with novel variants in IKZF5. Platelet ultrastructure and cytoskeleton were evaluated by immunofluorescent microscopy (IF) and found to be highly abnormal, demonstrating severe di...
Source: Platelets - May 19, 2020 Category: Hematology Tags: Platelets Source Type: research

Commissioned Review Series ONGOING SECTIONS.
Authors: PMID: 32406311 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - May 16, 2020 Category: Hematology Tags: Platelets Source Type: research

Flow-diverting stent and delayed intracranial bleeding: the case for discussing acquired von Willebrand disease.
Authors: Oran I Abstract A unique feature of the flow-diverting stent (FDS) has rendered it useful in the endovascular treatment of selected intracranial aneurysms for the last decade. Delayed aneurysmal rupture and intracranial parenchymal bleeding are two leading hemorrhagic complications after FDS. It has recently been shown for the first time that there is a relationship between FDS and reduced level of vWF activity in patients undergoing endovascular cerebral aneurysm treatment. Here, the current literature is reviewed in the context of this novel finding to propose an illustrative scenario that conceptually l...
Source: Platelets - May 16, 2020 Category: Hematology Tags: Platelets Source Type: research