Association between platelet counts and morbidity and mortality after endovascular repair for type B aortic dissection.
This study aimed to assess the association of postoperative platelet counts with early and late outcomes after thoracic endovascular aortic repair (TEVAR) for type B aortic dissection (TBAD). We retrospectively evaluated 892 patients with TBAD who underwent TEVAR from a prospectively maintained database. Postoperative nadir platelet counts were evaluated as a continuous variable, and a categorical variable (thrombocytopenia), which was defined as platelet count≤ the lowest 10% percentile (108 × 109/l). Multivariable logistic regression analyses were conducted to assess the impact of postoperative thrombo...
Source: Platelets - November 22, 2020 Category: Hematology Tags: Platelets Source Type: research

Zinc: an endogenous and exogenous regulator of platelet function during hemostasis and thrombosis.
Authors: Ahmed N, Lopes-Pires M, Pugh N Abstract Zinc (Zn2+) is an essential micronutrient and the second most abundant trace metal in the human body. The important role that Zn2+ plays in hemostasis is exemplified by platelet-related bleeding phenotypes coinciding with dietary Zn2+ deficiency. These phenotypes are rectified upon Zn2+ supplementation. Labile (unbound) Zn2+ is present in the plasma at micromolar levels, but is also detected in atherosclerotic plaques, and released from platelet α granules. Therefore, it is likely that localized Zn2+ concentrations are higher at sites of thrombosis and hemostas...
Source: Platelets - November 18, 2020 Category: Hematology Tags: Platelets Source Type: research

Technical and biological review of authorized medical devices for platelets-rich plasma preparation in the field of regenerative medicine.
Authors: Magalon J, Brandin T, Francois P, Degioanni C, De Maria L, Grimaud F, Veran J, Dignat-George F, Sabatier F Abstract Platelet-rich plasma (PRP) has seen increased interest and utilization over the past decade, particularly in the field of musculoskeletal disease. This growth has been accompanied by the development of medical devices to realize PRP preparation which includes blood collection, centrifugation, and PRP isolation. The final PRP composition is directly influenced by this preparation step and absence of biological quality control led to a lack of comparability between PRP products that could expla...
Source: Platelets - November 11, 2020 Category: Hematology Tags: Platelets Source Type: research

Validation of the DAPT score in large-scale consecutive and contemporary patients population in the real world.
This study enrolled 9,114 patients who had undergone PCI at Fuwai Hospital in 2013, adhered to DAPT and were event-free within the first 12 months following PCI. The endpoints included primary ischemic endpoints (major adverse cardiovascular and cerebrovascular events, and myocardial infarction and/or stent thrombosis), and bleeding endpoint from 12 through 24 months after PCI. Patients were classified into low (score
Source: Platelets - November 5, 2020 Category: Hematology Tags: Platelets Source Type: research

Pro-apoptotic properties and mitochondrial functionality in platelet-like-particles generated from low Aspirin-incubated Meg-01 cells.
Authors: Freixer G, Zekri-Nechar K, Zamorano-León JJ, Hugo-Martínez C, Butta NV, Monzón E, Recio MJ, Giner M, López-Farré A Abstract Long-term therapy with low Aspirin (ASA) dose is basis to prevent thrombotic acute events. However, the anti-platelet mechanisms of ASA remain not completely known. The aim was to analyze if in vitro exposure of human megakaryocytes to low ASA concentration may alter the apoptotic features of the newly formed platelets. Cultured Meg-01 cells, a human megakaryoblastic cell line, were stimulated to form platelets with 10 nmol/L phorbol 12-myristat...
Source: Platelets - October 29, 2020 Category: Hematology Tags: Platelets Source Type: research

Cerebral venous sinus thrombosis associated with spontaneous heparin-induced thrombocytopenia syndrome after total knee arthroplasty.
Authors: Hwang SR, Wang Y, Weil EL, Padmanabhan A, Warkentin TE, Pruthi RK Abstract Spontaneous heparin-induced thrombocytopenia (HIT) syndrome, characterized by clinical and serologic features of HIT despite the absence of proximate heparin exposure, can be triggered by total knee arthroplasty (TKA). A 56-year-old female receiving aspirin thromboprophylaxis post-TKA presented with aphasia and thrombocytopenia on post-operative day 11. Imaging studies revealed cerebral venous sinus thrombosis (CVST) and intravenous bivalirudin was initiated. Her serum tested strong-positive for IgG anti-PF4/polyanion complexes and ...
Source: Platelets - October 3, 2020 Category: Hematology Tags: Platelets Source Type: research

Loss of mDia1 and Fhod1 impacts platelet formation but not platelet function.
Authors: Zuidscherwoude M, Haining EJ, Simms VA, Watson S, Grygielska B, Hardy AT, Bacon A, Watson SP, Thomas SG Abstract An organized and dynamic cytoskeleton is required for platelet formation and function. Formins are a large family of actin regulatory proteins which are also able to regulate microtubule dynamics. There are four formin family members expressed in human and mouse megakaryocytes and platelets. We have previously shown that the actin polymerization activity of formin proteins is required for cytoskeletal dynamics and platelet spreading using a small molecule inhibitor. In the current study, we anal...
Source: Platelets - September 30, 2020 Category: Hematology Tags: Platelets Source Type: research

Comparison of antiplatelet antibody profiles between hepatitis C virus-associated immune thrombocytopenia and primary immune thrombocytopenia.
In conclusion, HCV-ITP patients had a high rate of positive antiplatelet antibody. The antibodies were associated with not only lower platelets but also leukopenia and splenomegaly. PMID: 32967492 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - September 26, 2020 Category: Hematology Tags: Platelets Source Type: research

Determination of fibrin clot growth and spatial thrombin propagation in the presence of different types of phospholipid surfaces.
Authors: Koltsova EM, Kuprash AD, Dashkevich NM, Vardanyan DM, Chernyakov AV, Kumskova MA, Nair SC, Srivastava A, Ataullakhanov FI, Panteleev MA, Balandina AN Abstract In this work, we present a new method-Thrombodynamics-4D-for the assessment of both plasma and platelet contributions to clotting. Thrombodynamics-4D potentially allows for the determination of plasma or platelet disorders and the effects of various drugs on plasma clotting or on platelet procoagulant function. In this assay, clot formation in platelet-rich plasma or platelet-free plasma supplemented with phospholipids is activated with tissue factor...
Source: Platelets - September 26, 2020 Category: Hematology Tags: Platelets Source Type: research

Treatment of immune thrombocytopenia (ITP) secondary to malignancy: a systematic review.
Authors: Podda GM, Fiorelli EM, Birocchi S, Rambaldi B, Di Chio MC, Casazza G, Cattaneo M Abstract Immune thrombocytopenia (ITP) can be associated with lymphoproliferative diseases (LPD) or solid tumors. A systematic review of published literature was conducted to evaluate response to treatment of ITP secondary to malignancy. Primary outcome was overall response (complete response+response) to first-line treatments [steroids alone or in combination with intravenous immunoglobulins (IVIg)]. Among secondary outcomes, overall response to second-line treatments [splenectomy, rituximab or thrombopoietin receptor agonist...
Source: Platelets - September 26, 2020 Category: Hematology Tags: Platelets Source Type: research

High-molecular-weight fucosylated glycosaminoglycan induces human platelet aggregation depending on αIIbβ3 and platelet secretion.
High-molecular-weight fucosylated glycosaminoglycan induces human platelet aggregation depending on αIIbβ3 and platelet secretion. Platelets. 2020 Sep 24;:1-9 Authors: Lin L, Yang L, Chen J, Zhou L, Li S, Gao N, Zhao J Abstract Fucosylated glycosaminoglycan (FG) from sea cucumbers has been reported to have anticoagulant effects via targeting intrinsic tenase. However, FG from natural source also potentially poses risks due to its FXIIa activation and platelet aggregating effects. Here, we found that the effect of FG on human platelet aggregation depended on both the sulfation pattern and cha...
Source: Platelets - September 26, 2020 Category: Hematology Tags: Platelets Source Type: research

Flow cytometry for platelets, EDTA or not EDTA: what is the answer?
Authors: Babuty A, Debord C, Béné MC, Fouassier M PMID: 32951494 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - September 23, 2020 Category: Hematology Tags: Platelets Source Type: research

In vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic tool.
Authors: Butov KR, Osipova EY, Mikhalkin NB, Trubina NM, Panteleev MA, Machlus KR Abstract Megakaryocytes (MKs) are relatively rare in bone marrow, comprising
Source: Platelets - September 17, 2020 Category: Hematology Tags: Platelets Source Type: research

Immunothrombosis and thromboinflammation in host defense and disease.
Authors: Martinod K, Deppermann C Abstract Platelets are increasingly being recognized for playing roles beyond thrombosis and hemostasis. Today we know that they mediate inflammation by direct interactions with innate immune cells or secretion of cytokines/chemokines. Here we review their interactions with neutrophils and monocytes/macrophages in infection and sepsis, stroke, myocardial infarction and venous thromboembolism. We discuss new roles for platelet surface receptors like GPVI or GPIb and also look at platelet contributions to the formation of neutrophil extracellular traps (NETs) as well as to deep vein ...
Source: Platelets - September 10, 2020 Category: Hematology Tags: Platelets Source Type: research

Immature platelet indices alongside procalcitonin for sensitive and specific identification of bacteremia in the intensive care unit.
Authors: Jones N, Tridente A, Dempsey-Hibbert NC Abstract Hematological markers that can be rapidly analyzed and regularly monitored during a patient's stay on ICU, and that can identify bacterial causes of sepsis are being extensively sought. The significance of platelets in early immunological responses provides justification for assessing their usefulness in the identification of bacteremia amongst sepsis patients. In this preliminary study, the full blood count, including the platelet count by impedance (PLT-I), Immature Platelet Fraction (IPF%) and absolute immature platelet count (AIPC), were analyzed in eigh...
Source: Platelets - September 10, 2020 Category: Hematology Tags: Platelets Source Type: research

Whole blood dynamic platelet aggregation counting and 1-year clinical outcomes in patients with coronary heart diseases treated with clopidogrel.
In conclusion, ADP-induced whole blood PARs via DPAC is feasible, which can predict the incidence of 1-year CEEs in patients with CHDs on treatment with clopidogrel. CYP2C19 gene polymorphism was associated with clopidogrel on-treatment platelet hyperresponsiveness. PMID: 32892681 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - September 9, 2020 Category: Hematology Tags: Platelets Source Type: research

Prognostic significance of thrombocytosis in lung cancer: a systematic review and meta-analysis.
In conclusion, this meta-analysis result suggested that thrombocytosis is a predictor of poor prognosis in lung cancer. PMID: 32892682 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - September 9, 2020 Category: Hematology Tags: Platelets Source Type: research

Selective inhibition of thromboinflammation in COVID-19 by Btk inhibitors.
Authors: Siess W, Hundelshausen PV, Lorenz R PMID: 32892684 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - September 9, 2020 Category: Hematology Tags: Platelets Source Type: research

High extracellular phosphate increases platelet polyphosphate content.
Authors: Abbasian N, Harper MT Abstract Platelet-derived extracellular polyphosphate (PolyP) is a major mediator of thrombosis. PolyP is a linear chain of inorganic phosphate (P i ) and is stored in platelet dense granules. P i enters cells from the extracellular fluid through phosphate transporters and may be stored as PolyP. Here we show that high extracellular P i concentration in vitro increases platelet PolyP content, in a manner dependent on phosphate transporters, IP6K and V-type ATPases. The increased PolyP also enhanced PolyP-dependent coagulation in platelet-rich plasma. These data suggest a mechanistic l...
Source: Platelets - September 9, 2020 Category: Hematology Tags: Platelets Source Type: research

Platelets and renal failure in the SARS-CoV-2 syndrome.
Authors: Taha M, Sano D, Hanoudi S, Esber Z, Elahi M, Gabali A, Chopra T, Draghici S, Samavati L Abstract The coronavirus disease 19 (COVID-19) is a highly transmittable viral infection caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SARS-CoV-2 utilizes metallocarboxyl peptidase angiotensin receptor (ACE) 2 to gain entry into human cells. Activation of several proteases facilitates the interaction of viral spike proteins (S1) and ACE2 receptor. This leads to cleavage of host ACE2 receptors. ACE2 activity counterbalances the angiotensin II effect, its loss may lead to elevated angiotensin...
Source: Platelets - September 9, 2020 Category: Hematology Tags: Platelets Source Type: research

Whole blood flow cytometry protocol for the assessment of platelet phenotype, function, and cellular interactions.
Authors: Yaw HP, Van den Helm S, Linden M, Monagle P, Ignjatovic V Abstract Platelets are a key component of the hemostatic system and their roles in inflammation via interactions with leukocytes have also gained attention in recent years. Changes in platelet phenotype and function can cause bleeding and/or thrombosis and, as such, monitoring platelet-specific changes is crucial to assessing hemostasis in the clinical setting. Currently, available platelet function tests such as platelet aggregometry and thromboelastography require a large volume of blood, which is a major limitation for the pediatric population. W...
Source: Platelets - September 7, 2020 Category: Hematology Tags: Platelets Source Type: research

Contribution of platelet-derived microRNAs to serum microRNAs in healthy men.
Authors: Wakabayashi I, Sotoda Y, Eguchi R Abstract Platelets are a major source of microRNAs (miRNAs) in blood. Relationships between circulating platelet-derived miRNAs were investigated to elucidate their significance as biomarkers. Total miRNAs in serum were analyzed using the 3D-Gene miRNA Oligo chip. Among 22 miRNAs that are included in platelets and play functional roles, sufficient miRNA levels for comparison were detected for 11 miRNAs (let-7b-5p, miR-16-5p, miR-17-5p, miR-24-3p, miR-107, miR-126-3p, miR-150-3p, miR-191-5p, miR-197-3p, miR-223-3p, and miR-326). Among 55 pairs prepared by these miRNAs, rela...
Source: Platelets - September 2, 2020 Category: Hematology Tags: Platelets Source Type: research

Optimized tool for evaluation of platelet function measured by impedance aggregometry.
Authors: Schultz-Lebahn A, Skipper MT, Hvas AM, Larsen OH Abstract Platelet aggregation measured by impedance aggregometry is highly dependent on platelet count. We previously developed a tool to interpret impedance aggregometry based on the strong linear correlation between platelet counts and platelet aggregation at reduced platelet counts. The present study aimed to optimize the tool by expanding the model to include normal platelet counts. We combined data from three previous studies on 266 healthy individuals measuring impedance aggregometry with four agonists (collagen, adenosine diphosphate, thrombin recepto...
Source: Platelets - September 2, 2020 Category: Hematology Tags: Platelets Source Type: research

A proof of evidence supporting abnormal immunothrombosis in severe COVID-19: naked megakaryocyte nuclei increase in the bone marrow and lungs of critically ill patients.
Authors: Roncati L, Ligabue G, Nasillo V, Lusenti B, Gennari W, Fabbiani L, Malagoli C, Gallo G, Giovanella S, Lupi M, Salviato T, Paolini A, Costantini M, Trenti T, Maiorana A Abstract Coronavirus disease 2019 (COVID-19) is a global public health emergency with many clinical facets, and new knowledge about its pathogenetic mechanisms is deemed necessary; among these, there are certainly coagulation disorders. In the history of medicine, autopsies and tissue sampling have played a fundamental role in order to understand the pathogenesis of emerging diseases, including infectious ones; compared to the past, histopat...
Source: Platelets - August 30, 2020 Category: Hematology Tags: Platelets Source Type: research

Evening aspirin intake results in higher levels of platelet inhibition and a reduction in reticulated platelets - a window of opportunity for patients with cardiovascular disease?
Authors: van Diemen JJK, Madsen MC, Vrancken P, de Bie K, van der Bom JG, Veen G, Bonten TN, Fuijkschot WW, Smulders YM, Thijs A Abstract Cardiovascular events occur most frequently in the early morning. Similarly, the release of reticulated platelets (RP) by megakaryocytes has a peak in the late night and early morning. Which aspirin regimen most effectively inhibits platelets during these critical hours is unknown. Hence, the primary objective of this trial was to assess platelet function and RP levels at 8.00 AM, in stable cardiovascular (CVD) patients, during three different aspirin regimens. In this open-label...
Source: Platelets - August 27, 2020 Category: Hematology Tags: Platelets Source Type: research

High-efficiency unassisted transfection of platelets with naked double-stranded miRNAs modulates signal-activated translation and platelet function.
Authors: Lazar S, Wurtzel JGT, Chen X, Ma P, Goldfinger LE Abstract We sought novel approaches to improve transfection efficiencies of microRNAs (miRNAs) in platelets, and to apply these approaches to investigate the roles of miRNAs in regulating signal-activated protein translation and functional effects. We found that ex vivo human platelets support gymnosis---internalization of ectopic miRNAs following co-incubation in the absence of conventional transfection reagents or schemes---and subsequently incorporate transfected miRNA into ARGONAUTE2 (AGO2)-based RNA-induced silencing complexes (RISC). Thrombin/fibrinog...
Source: Platelets - August 27, 2020 Category: Hematology Tags: Platelets Source Type: research

Acquired platelet defect associated with gabapentin treatment: a case-report.
Authors: Baglo T, Pouplard C, Valentin JB, Guéry EA, Gruel Y, Vayne C Abstract Gabapentin, a structural analog of gamma-aminobutyric acid, is used to treat peripheral neuropathic pain. Here we report the first case of platelet function disorder associated with gabapentin treatment in a 44-year-old woman without a history of bleeding. She presented with mucocutaneous bleeding approximately 1 month after initiation of gabapentin and platelet function tests showed no aggregation to arachidonic acid and epinephrine, a defective response to ADP and a slightly decreased response to collagen. Gabapentin's impu...
Source: Platelets - August 25, 2020 Category: Hematology Tags: Platelets Source Type: research

Reversible cross-tolerance to platelet-activating factor signaling by bacterial toxins.
Authors: Abhilasha KV, Sumanth MS, Thyagarajan A, Sahu RP, Kemparaju K, Marathe GK Abstract Bacterial toxins signaling through Toll-like receptors (TLRs) are implicated in the pathogenesis of many inflammatory diseases. Among the toxins, lipopolysaccharide (LPS) exerts its action via TLR-4 while lipoteichoic acid (LTA) and bacterial lipoproteins such as Braun lipoprotein (BLP) or its synthetic analogue Pam3CSK4 act through TLR-2. Part of the TLR mediated pathogenicity is believed to stem from endogenously biosynthesized platelet-activating factor (PAF)- a potent inflammatory phospholipid acting through PAF-receptor...
Source: Platelets - August 25, 2020 Category: Hematology Tags: Platelets Source Type: research

Platelet-rich plasma promotes bone formation, restrains adipogenesis and accelerates vascularization to relieve steroids-induced osteonecrosis of the femoral head.
In this study, we investigated the effects and potential mechanisms of Platelet-rich plasma (PRP) on SANFH. Sixty Sprague-Dawley rats were randomly divided into the control, PRP donor, model, and PRP groups. Compared to the model group, PRP treatment significantly increased the hemorheological indexes and serum levels of bone gla-protein (BGP) and vascular endothelial growth factor (VEGF), while decreased the levels of triglyceride (TG) and total cholesterol (TC). Meanwhile, Micro-CT and histopathological stain (Hematoxylin-eosin and Alcian blue-hematoxylin/orange G staining) were performed on the femoral head for morpholo...
Source: Platelets - August 25, 2020 Category: Hematology Tags: Platelets Source Type: research

Myelofibrosis in ITP and with TPO-RA - time to rethink?
Authors: Thachil J PMID: 32835570 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - August 25, 2020 Category: Hematology Tags: Platelets Source Type: research

Protease-activated receptor 4 causes Akt phosphorylation independently of PI3 kinase pathways.
Authors: Dangelmaier C, Kunapuli SP Abstract PI-3 Kinase plays an important role in platelet activation mainly through regulation of RASA3. Akt phosphorylation is an indicator for the activity of PI3 kinase. The aim of this study is to characterize the pathways leading to Akt phosphorylation in platelets. We performed concentration response curves of LY294002, a pan-PI3 kinase inhibitor, on platelet aggregation and Akt phosphorylation, in washed human and mouse platelets. At concentrations as low as 3.12 µM, LY294002 abolished Akt phosphorylation induced by 2MeSADP and SFLLRN, but not by AYPGKF. It requi...
Source: Platelets - August 21, 2020 Category: Hematology Tags: Platelets Source Type: research

Investigation of fibrinogen in early bleeding of patients with newly diagnosed acute promyelocytic leukemia.
This study investigated the role of fibrinogen concentrations in early hemorrhage and overall survival (OS) of APL patients. Laboratory and clinical data, including fibrinogen concentrations and other coagulation indexes, bleeding events, and survival data, of 198 patients newly diagnosed with APL from February 2012 to December 2017 were extracted from patient records and retrospectively investigated. Patients with moderate/severe bleeding had significantly lower median fibrinogen concentrations (p = .023), higher Chinese disseminated intravascular coagulation scoring system (CDSS) (p 1.6 g/L may help to prevent ...
Source: Platelets - August 18, 2020 Category: Hematology Tags: Platelets Source Type: research

Association of lipoprotein(a) with platelet aggregation and thrombogenicity in patients undergoing percutaneous coronary intervention.
This study aimed to evaluate the association of lipoprotein(a) levels with platelet aggregation and thrombogenicity in patients undergoing percutaneous coronary intervention (PCI), and to investigate the ischemic outcome on this population. Lipoprotein(a) and modified thrombelastography were measured in 6601 consecutive patients underwent PCI on dual antiplatelet therapy. Cox proportional regression analysis was applied to illustrate the ischemic events in a 2-year follow up. The mean levels of lipoprotein(a) were 29.0 mg/dl. Patients with higher lipoprotein(a) levels had significantly accelerated fibrin generation (lower ...
Source: Platelets - August 14, 2020 Category: Hematology Tags: Platelets Source Type: research

Platelet count and disease - editorial policy.
Authors: Harrison P, Lordkipanidzé M, Frelinger Iii AL, Thomas MR, Watson SP PMID: 32762569 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - August 13, 2020 Category: Hematology Tags: Platelets Source Type: research

Immature cell fractions after cessation of chronic P2Y12-inhibition in patients with coronary artery diseases.
Authors: Jäger B, Vargas KG, Haller PM, Stojkovic S, Kaufmann CC, Freynhofer M, Quehenberger P, Wagner O, Wojta J, Huber K Abstract Changes in circulating cell populations may promote ischemic events that occur soon after discontinuation of P2Y12-inhibition. The aim of the study was to track the course of thrombopoietic and erythropoietic cells in patients with coronary artery diseases (CAD) after planned and physician-driven cessation of chronic P2Y12-inhibition (clopidogrel 75 mg OD, or prasugrel 10 mg OD, or ticagrelor 90 mg BID). Cell fractions were determined in 62 patients at baseline (the last day of P2...
Source: Platelets - August 13, 2020 Category: Hematology Tags: Platelets Source Type: research

Catastrophic antiphospholipid syndrome refractory to high-dose intravenous immunoglobulin responsive to therapeutic plasma exchange.
Authors: James TE, Martin LJ, Warkentin TE, Crowther MA Abstract Catastrophic antiphospholipid syndrome (CAPS) involves sudden multiorgan dysfunction from thrombosis due to antibodies that cause platelet activation and endothelial dysfunction. Treatment variably combines anticoagulation, corticosteroid use, therapeutic plasma exchange (TPE), and high-dose intravenous immunoglobulin (IVIG). A 42-year-old male with antiphospholipid syndrome (APS) presented with severe thrombocytopenia, encephalopathy, cardiac ischemia, and acral purpuric cutaneous lesions. CAPS was identified and he received heparin infusion, methylp...
Source: Platelets - August 13, 2020 Category: Hematology Tags: Platelets Source Type: research

Factor Xa and thrombin induce endothelial progenitor cell activation. The effect of direct oral anticoagulants.
Authors: Papadaki S, Sidiropoulou S, Moschonas IC, Tselepis AD Abstract Factor Xa (FXa) and thrombin exert non-hemostatic cellular actions primarily mediated through protease-activated receptors (PARs). We investigated the effect of FXa and thrombin on human late-outgrowth endothelial cells (OECs), a type of endothelial progenitor cells (EPCs), and on human umbilical vein endothelial cells (HUVECs). The effect of direct oral anticoagulants (DOACs), rivaroxaban and dabigatran, was also studied. The membrane expression of intercellular adhesion molecule-1 (ICAM-1) and the secretion of monocyte chemoattractant protein...
Source: Platelets - August 13, 2020 Category: Hematology Tags: Platelets Source Type: research

Factors Involved in the onset of infection following bacterially contaminated platelet transfusions.
Authors: Wilson-Nieuwenhuis J, El-Mohtadi M, Edwards K, Whitehead K, Dempsey-Hibbert N Abstract Transfusion of platelet concentrates (PCs) is associated with several adverse patient reactions, the most common of which are febrile non-hemolytic transfusion reactions (FNHTRs) and transfusion-associated bacterial-infection/transfusion-associated sepsis (T-ABI/TA-S). Diagnosis of T-ABI/T-AS requires a positive blood culture (BC) result from the transfusion recipient and also a positive identification of bacterial contamination within a test aliquot of the transfused PC. In a significant number of cases, clinical sympto...
Source: Platelets - August 13, 2020 Category: Hematology Tags: Platelets Source Type: research

Isolation of murine bone marrow by centrifugation or flushing for the analysis of hematopoietic cells - a comparative study.
Authors: Heib T, Gross C, Müller ML, Stegner D, Pleines I Abstract Investigation of the bone marrow as the main compartment of hematopoiesis is critical in many research fields. Here, we adapted a centrifugation-based method for the isolation of murine bone marrow and compared it to the traditional flushing method. Analysis of primary hematopoietic stem cells, immune cells, and megakaryocytes revealed a comparable distribution of cellular (sub)populations. Furthermore, in vitro differentiated megakaryocytes displayed unaltered proplatelet formation. Strikingly, bone marrow isolation by centrifugation was consi...
Source: Platelets - July 31, 2020 Category: Hematology Tags: Platelets Source Type: research

Editorial.
Authors: Ghevaert C PMID: 32697612 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - July 24, 2020 Category: Hematology Tags: Platelets Source Type: research

Effect of differently coated silver nanoparticles on hemostasis.
This study aimed to evaluate comprehensively AgNPs effects on hemostasis in human blood by exploiting combination of affordable and clinically relevant techniques. Differently stabilized AgNPs were prepared using sodium bis(2-ethylhexyl)sulphosuccinate (AOT), polyvinylpyrrolidone (PVP), poly-L-lysine (PLL), and bovine serum albumin (BSA) as coating agents. They were tested for hemolytic activity, induction of platelet aggregation, plasmatic coagulation, thrombin generation, and hemostasis in whole blood. All AgNPs were found to cause dose-dependent hemolysis. The BSA-, AOT-, and PVP-coated AgNPs delayed plasmatic coagulati...
Source: Platelets - July 18, 2020 Category: Hematology Tags: Platelets Source Type: research

Aspirin after PCI: in the twilight of its years?
Authors: Parker WAE PMID: 32669035 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - July 18, 2020 Category: Hematology Tags: Platelets Source Type: research

Potential contrasting effects of platelets on the migration and invasion of sarcomas versus carcinomas.
This study was undertaken to simultaneously compare the effects of platelets on murine and human sarcomas and carcinomas. In contrast to their effect on carcinomas, platelets inhibited the invasion of some murine- and all human sarcomas tested in vitro. Further invasion studies with TGFβ treatment only partially recapitulated the results seen with whole platelets. In a spontaneous tumor growth and lung metastasis model, platelets promoted 4T1 mammary carcinoma metastasis but not MCA-1 fibrosarcoma metastasis. Gene expression analysis of the platelet-promoted MDA-MB-231 breast carcinoma, and the platelet-inhibited HT10...
Source: Platelets - July 17, 2020 Category: Hematology Tags: Platelets Source Type: research

Risk of major adverse cardiovascular events of CYP2C19 loss-of-function genotype guided prasugrel/ticagrelor vs clopidogrel therapy for acute coronary syndrome patients undergoing percutaneous coronary intervention: a meta-analysis.
Authors: Biswas M, Kali MSK, Biswas TK, Ibrahim B Abstract The most effective antiplatelet treatments for acute coronary syndrome (ACS) patients carrying CYP2C19 loss-of-function (LoF) alleles undergoing percutaneous coronary intervention (PCI) is still debating and conflicting. It was aimed to compare the efficacy and safety endpoints for these patients treated with alternative P2Y12 receptor blockers (e.g. prasugrel or ticagrelor) against clopidogrel. Literature was searched in PubMed, Cochrane library, Synapse and 1000 Genomes databases following PRISMA guidelines for identifying relevant studies. Aggregated ris...
Source: Platelets - July 17, 2020 Category: Hematology Tags: Platelets Source Type: research

Statins potentiate the antibacterial effect of platelets on Staphylococcus aureus.
Authors: Hannachi N, Fournier PE, Martel H, Habib G, Camoin-Jau L Abstract Platelets have largely demonstrated their implication in anti-infectious immunity. This effect is ensured by the secreted molecules stored mostly in platelet alpha granules. Previous studies have reported that Staphylococcus aureus showed sensitivity to this antibacterial effect of platelets. Statins, for their part, have shown a modulating effect on platelet activation. Furthermore, several studies have reported a protective effect of statins in staphylococcal endocarditis. The aim of this study was to investigate the influence of statins o...
Source: Platelets - July 17, 2020 Category: Hematology Tags: Platelets Source Type: research

Diagnostic challenges in von Willebrand disease. Report of two cases with emphasis on multimeric and molecular analysis.
We report two cases of infrequent inherited variants of VWD with unclear preliminary results with the test panel available at the time of first consultation and that were finally diagnosed as a VWD type 2A/IID with a c.8318 G > C, p.Cys2773Ser mutation and a VWD type 2M with c.4225 T > G, p.Val1409Phe mutation, respectively. The description of these two cases highlights that despite the limited diagnostic panel for the evaluation of von Willebrand Factor (VWF) functionality, the multimeric analysis and genetic family studies were fundamental tools to achieve the final diagnosis. PMID...
Source: Platelets - July 17, 2020 Category: Hematology Tags: Platelets Source Type: research

Relation between mutations in the 5' UTR of ANKRD26 gene and inherited thrombocytopenia with predisposition to myeloid malignancies. An Egyptian study.
Authors: Zidan NI, AbdElmonem DM, Elsheikh HM, Metwally EA, Mokhtar WA, Osman GM Abstract Inherited thrombocytopenias are a heterogeneous group of diseases characterized by a reduced number of platelets and a bleeding tendency that ranges from very mild to life threatening especially in surgery. Mutations in the 5' untranslated region (UTR) of Ankirin repeat domain 26 (ANKRD26) are responsible for autosomal-dominant form of thrombocytopenia, that is known as ANKRD26-related thrombocytopenia (ANKRD26 RT), characterized by a moderate thrombocytopenia with mild propensity to bleeding and predisposition to hematologica...
Source: Platelets - July 15, 2020 Category: Hematology Tags: Platelets Source Type: research

Efficacy and safety of short-term 1-3 months versus standard 12 months dual antiplatelet therapy in patients undergoing percutaneous coronary intervention: a meta-analysis of randomized clinical trials.
In conclusion, the short-term DAPT can reduce the risk of bleeding compared with standard DAPT, without increasing the risk of death or ischemia (Registered by PROSPERO, CRD42020153881). PMID: 32627616 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - July 8, 2020 Category: Hematology Tags: Platelets Source Type: research

Absence of bleeding upon dual antiplatelet therapy in a patient with a immune GPVI deficiency.
We report the case of a patient presenting an acquired GPVI deficiency different from those previously described: (i) he is male whereas all previous cases were female, (ii) he is heterozygous for a mutation in α (alpha)-actinin-1 gene and (iii) he was treated with dual antiplatelet therapy with no haemorrhagic manifestation. PMID: 32627625 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - July 8, 2020 Category: Hematology Tags: Platelets Source Type: research

Platelet count and function in umbilical cord blood versus peripheral blood in term neonates.
Authors: Grevsen AK, Hviid CVB, Hansen AK, Hvas AM Abstract Platelet function in neonates is sparsely investigated. The majority of previous studies investigated platelets in umbilical cord (UC) blood rather than in peripheral blood. We included 20 term neonates and sampled UC blood and peripheral blood within 20 min and 24 h after birth. Platelet count and mean platelet volume (MPV) were measured. Platelet surface glycoproteins (GP) and platelet activation (bound fibrinogen, CD63 and p-selectin) after agonist stimulation were examined by flow cytometry. Platelet aggregation was evaluated by impedance agg...
Source: Platelets - July 8, 2020 Category: Hematology Tags: Platelets Source Type: research