Immature platelet fraction related parameters in the differential diagnosis of thrombocytopenia.
This study aimed to analyze the efficacy of immature platelet fraction (IPF) related parameters, including the IPF count (IPF#), IPF percentage (IPF%) and highly fluorescence IPF percentage (H-IPF%), measured by XN-9000, in the differential diagnosis of thrombocytopenia. One hundred and twenty healthy volunteers were enrolled in the healthy control (HC) group, and 180 thrombocytopenia patients were grouped into either the increased destruction (ID) group or the decreased production (DP) group according to their final diagnosis. IPF# was significantly lower in the DP group than in the ID and HC groups (P
Source: Platelets - October 18, 2019 Category: Hematology Tags: Platelets Source Type: research

Platelet consumption and hyperreactivity coexist in experimental traumatic hemorrhagic model.
Authors: Wannberg M, Miao X, Li N, Wikman A, Wahlgren CM Abstract Introduction Platelets are critical for hemostasis, and a low platelet count predicts mortality in trauma. The role of platelet dysfunction in severe traumatic hemorrhage and coagulopathy needs to be further defined. The aim of this study was to evaluate the platelet function in a new model of experimental traumatic hemorrhage. Material and methods New Zealand white rabbits (n = 10) were subjected to tracheostomy and trauma laparotomy, and then bilateral femur fractures with 40% hemorrhage of their estimated blood volume. Arterial blood gases, standa...
Source: Platelets - October 17, 2019 Category: Hematology Tags: Platelets Source Type: research

Fibrinogen levels are associated with bleeding in patients with primary immune thrombocytopenia.
Authors: Xu M, Luo L, Du M, Tang L, Hu Y, Mei H Abstract Bleeding is the most common clinical symptom and the leading cause of death in patients with primary immune thrombocytopenia (ITP). Our research intends to verify the role of fibrinogen levels as independent determinants of bleeding. We retrospectively analyzed the relationship between fibrinogen levels and bleeding events in 463 patients. Additionally, we confirmed the impact of fibrinogen level on clot firmness in 25 patients via thrombelastography (TEG). Fibrinogen levels (median and inter-quartile range, IQR) were significantly different (p
Source: Platelets - October 17, 2019 Category: Hematology Tags: Platelets Source Type: research

Recurrent stent thrombosis in a patient with acute coronary syndrome and ischemic colitis: between life-threatening thrombosis and life-threatening bleeding.
We present a challenging case of recurrent acute coronary syndrome, in the context of very high thrombotic risk due to concomitant inflammatory disease. Although no general guidelines exist for the switch between antiplatelet agents, particularly in the acute setting, in specific cases, the availability of different orally- and i.v.-acting agents and platelet function tests may allow to discriminate among multiple possible mechanisms of drug failure or side effects in the individual patient. PMID: 31607192 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - October 15, 2019 Category: Hematology Tags: Platelets Source Type: research

Successful management of a pregnant woman with severe ANKRD26-related thrombocytopenia and anti-HPA-5b alloimmunization.
Authors: Lazaro E, Houssin C, Sentilhes L, Blouin L, Fiore M PMID: 31607198 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - October 15, 2019 Category: Hematology Tags: Platelets Source Type: research

Influence of antiretroviral therapy and cardiovascular disease on the immature platelet fraction in patients living with HIV.
Authors: Goedel A, Müller S, Schwerdtfeger C, Zink A, Noe S, Bongiovanni D, Haller B, Spinner CD, Bernlochner I Abstract Cardiovascular disease is an important contributor to morbidity and mortality in people living with HIV . The immature platelet fraction (IPF) is increased in HIV-negative patients with cardiovascular disease and evidence suggests that an enlarged IPF is associated with adverse cardiovascular events. In this multi-center observational study, we aimed to investigate how the IPF in people living with HIV is influenced by antiretroviral therapy and cardiovascular disease. Subjects without cardi...
Source: Platelets - October 15, 2019 Category: Hematology Tags: Platelets Source Type: research

How useful are ferric chloride models of arterial thrombosis?
Authors: Grover SP, Mackman N Abstract The ferric chloride models of arterial thrombosis are useful tools with which to investigate the cellular and molecular mechanisms that contribute to arterial thrombosis. Recent insights have, however, revealed the complex and multifaceted mechanism by which ferric chloride induces thrombus formation. Here, we discuss the strengths and weaknesses of the ferric chloride models of arterial thrombosis. Particular focus is given to the phenotypes of different knockout mice in the ferric chloride models and how these compare to other models with independent modes of initiation. Fur...
Source: Platelets - October 15, 2019 Category: Hematology Tags: Platelets Source Type: research

Pharmacodynamic comparison of low-dose ticagrelor to low-dose prasugrel in patients with prior myocardial infarction: the ALTIC-2 study.
Authors: Alexopoulos D, Sfantou D, Lianos I, Pappas C, Revela I, Triantafyllidi H, Ikonomidis I Abstract Given that patients with prior myocardial infarction and features of high ischemic and low bleeding risk may benefit by extending dual antiplatelet therapy beyond 1 year, we aimed of assessing platelet reactivity provided by ticagrelor 60 mg bid versus prasugrel 5 mg od in 20 such patients participating in a randomized, crossover study. The primary end point of platelet reactivity at the end of the two treatment periods (by VerifyNow, in PRU) was significantly lower for ticagrelor (31.9 PRU [95% CI 12.3-51.4]) c...
Source: Platelets - October 15, 2019 Category: Hematology Tags: Platelets Source Type: research

More about the combination of rituximab, cyclosporine and dexamethasone in the treatment of chronic ITP. A useful option on an environment with limited resources.
Authors: Thabet AF, Moeen SM Abstract Treatment of chronic primary immune thrombocytopenia (ITP) is challenging especially with limited resources and therapy-related complications. This prospective interventional study assessed the efficacy and safety of triple therapy(TT4); a combination treatment of oral dexamethasone 40 mg for days1-4, oral cyclosporine A 2-3 mg/kg daily for 28 consecutive days and intravenous rituximab 100 mg for days7,14,21 and 28 among 40 patients with ITP who failed previous 2 or more treatment options. Our aim was to maintain platelet count ≥ 30 X 109/L without any obvious bleeding at an...
Source: Platelets - October 13, 2019 Category: Hematology Tags: Platelets Source Type: research

Effects of cigarette smoking on older chinese men treated with clopidogrel monotherapy or aspirin monotherapy: a prospective study.
Authors: Cai Y, Xu W, Liu H, Wang F, Duan L, Li H, Li M, Li Y, Han L, Xiao H Abstract We investigated the comparative effects of smoking status on outcomes in older Chinese men receiving aspirin or clopidogrel monotherapy. This was a prospective observational study of outcomes in 668 men aged ≥ 60 years undergoing annual health examination in the Chinese People's Liberation Army General Hospital from March-April 2017. All patients received regular treatment with aspirin or clopidogrel. Platelet aggregation and phenotyping for rs762551 were measured in all patients. We recorded all major adverse cardiovascul...
Source: Platelets - September 28, 2019 Category: Hematology Tags: Platelets Source Type: research

Platelet surface marker analysis by mass cytometry.
Authors: Blair TA, Frelinger AL Abstract Mass cytometry is a next generation flow cytometry technology which analyzes cells one at a time (up to 1000/sec) using mass spectrometry to detect probes labeled with rare-earth metals. Rare-earth metals detected by mass spectrometry have extremely low backgrounds and can be identified with high resolution enabling the routine simultaneous detection of more than 45 probes on each cell without the need for complex compensation matrices. Here we describe a panel of 14 platelet-specific metal-conjugated antibodies (targeting cluster of differentiation [CD] 9, CD29, CD31, CD36,...
Source: Platelets - September 25, 2019 Category: Hematology Tags: Platelets Source Type: research

Megakaryocyte polyploidization: role in platelet production.
Authors: Vainchenker W, Raslova H Abstract Mammal megakaryocytes (MK) undergo polyploidization during their differentiation. This process leads to a marked increase in the MK size and of their cytoplasm. Contrary to division by classical mitosis, ploidization allows an economical manner to produce platelets as they arise from the fragmentation of the MK cytoplasm. The platelet production in vivo correlates to the entire MK cytoplasm mass that depends both upon the number of MKs and their size. Polyploidization occurs by several rounds of DNA replication with at the end of each round an aborted mitosis at late phase...
Source: Platelets - September 25, 2019 Category: Hematology Tags: Platelets Source Type: research

Human platelet antigen polymorphisms and the risk of chronic Chagas disease cardiomyopathy.
In conclusion, HPA-3 variant could be a protection factor for CCC in the female patients. PMID: 31538834 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - September 21, 2019 Category: Hematology Tags: Platelets Source Type: research

Weight as an assay-independent predictor of poor response to enteric aspirin in cardiovascular patients.
In this study, we determine the effects of weight on different assays of platelet function. The response to aspirin was determined in 138 cardiology patients using serum thromboxane, arachidonic acid-induced platelet aggregation and VerifyNow©. Twenty-five percent of patients showed an inadequate response to aspirin in at least one assay on the initial test. After ensuring patient compliance only 5% of patients were considered to be non-responders. Only 9% of non-responders were non-responsive in all three assays. When switched to plain aspirin, only 2% of patients were non-responsive. All patients responded adequatel...
Source: Platelets - September 20, 2019 Category: Hematology Tags: Platelets Source Type: research

Modulators of platelet function in aging.
Authors: Iyer KS, Dayal S Abstract Platelets are small, anucleated effector cells that play an important role in linking the hemostatic and inflammatory processes in the body. Platelet function is known to be altered under various inflammatory conditions including aging. A gain in platelet function during aging can increase the risk of thrombotic events, such as stroke and acute myocardial infarction. Anti-platelet therapy is designed to reduce risk of serious cerebrovascular and cardiovascular events, but the adverse consequences of therapy, such as risk for bleeding increases with aging as well. Age-associated co...
Source: Platelets - September 18, 2019 Category: Hematology Tags: Platelets Source Type: research

Evaluation of the in vivo wound healing potential of the lipid fraction from activated platelet-rich plasma.
Authors: Cakin MC, Ozdemir B, Kaya-Dagistanli F, Arkan H, Bahtiyar N, Anapali M, Akbas F, Onaran I Abstract Previous in vitro studies suggest a direct relevance for the peptide-free lipid fraction (LF) of platelet-rich plasma (PRP) in biological mechanisms related to wound healing. However, there are no scientific reports to date on the wound healing activities of this lipid component in vivo. Thus, the present study provides a scientific evaluation for the wound healing potential of the lipid portion of the activated PRP. For the wound healing activity assessment, in vivo full-thickness excisional wounds were crea...
Source: Platelets - September 18, 2019 Category: Hematology Tags: Platelets Source Type: research

Pseudothrombocytopenia: a case of platelet satellitism and phagocytosis by neutrophils.
Authors: Sousa SM, Sousa TM, Silva CF, Mendes CC Abstract Thrombocytopenia is a clinically important condition that can lead to several problems when not correctly diagnosed. A decrease of platelet counts due to an in vitro phenomenon, Pseudothrombocytopenia, can be misunderstood and unnecessarily treated. The present case study describes a 57-year-old male with a history of pancreas adenocarcinoma and a current Staphylococcus aureus infection without any signs or symptoms that could explain the low levels of platelets obtained after blood analysis. Blood smear evaluation detected both platelet satellitism and phag...
Source: Platelets - September 14, 2019 Category: Hematology Tags: Platelets Source Type: research

Impaired platelet reactivity in patients with septic shock: a proof-of-concept study.
Authors: Fogagnolo A, Taccone FS, Campo G, Montanari G, Capatti B, Ferraro G, Erriquez A, Ragazzi R, Creteur J, Volta CA, Spadaro S Abstract Coagulation disorders and thrombocytopenia are common in patients with septic shock, but only few studies have focused on platelet variables beyond platelet count. The aim of this study was to evaluate whether platelets reactivity predicts sepsis-induced thrombocytopenia in patients with septic shock. We therefore enrolled consecutive patients with septic shock and platelets count>150*103/μL on the day of the diagnosis. Platelets reactivity tests were performed daily fro...
Source: Platelets - September 14, 2019 Category: Hematology Tags: Platelets Source Type: research

Platelet count and mortality of H7N9 infected patients in Guangdong, China.
This study demonstrates that using platelet count to predict the fatality of H7N9 is significant, and lower platelet counts of H7N9 patients were associated with higher risk of mortality of H7N9 patients, which may need to be taken into consideration when planning clinical treatment. PMID: 31509040 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - September 13, 2019 Category: Hematology Tags: Platelets Source Type: research

Inhibition of agonist-induced platelet aggregation by magnesium sulfate warrants its use as an alternative in vitro anticoagulant in pseudothrombocytopenia.
Authors: Mannuß S, Schuff-Werner P, Dreißiger K, Burstein C Abstract MgSO4 is effective in preventing spontaneous in vitro platelet agglutination in anticoagulant-induced pseudothrombocytopenia (PTCP). In order to learn more about its potential as an in vitro anticoagulant, platelets from MgSO4-anticoagulated blood were stimulated by several differentially-acting agonists (ADP, ARA, TRAP, epinephrine, collagen and ristocetin). Platelet aggregation in blood samples from 11 and 17 volunteers was measured by light-transmission aggregometry (LTA) according to Born and impedance aggregometry (MultiplateTM), ...
Source: Platelets - September 13, 2019 Category: Hematology Tags: Platelets Source Type: research

Stimulation of mTORC2 by integrin αIIbβ3 is required for PI3Kβ-dependent activation of Akt but is dispensable for platelet spreading on fibrinogen.
In this study we found that during integrin αIIbβ3 outside-in signaling PI3Kβ-dependent phosphorylation of Akt on Serine473 is mediated by the mammalian target of rapamycin complex 2 (mTORC2). The activity of mTORC2 is stimulated upon platelet adhesion to fibrinogen, as documented by increased autophosphorylation. However, mTORC2 activation downstream of integrin αIIbβ3 is PI3Kβ-independent. Inhibition of mTORC2, but not mTORC1, also prevents Akt phosphorylation of Threonine308 and affects Akt activity, resulting in the inhibition of GSK3α/β phosphorylation. Nevertheless, mTORC2 o...
Source: Platelets - September 13, 2019 Category: Hematology Tags: Platelets Source Type: research

Platelet yield and yield consistency for six single-spin methods of platelet rich plasma preparation.
Authors: Harrison TE, Bowler J, Levins TN, Cheng AL, Reeves KD Abstract Single-spin methods of preparation of platelet-rich plasma are used widely in private practice, yet they have not been extensively studied and compared. The use of platelet-rich plasma (PRP) by the private practitioner can be facilitated by efficient and predictable PRP preparation. The primary purpose of this study was to study common methods of single-spin PRP preparation to determine their efficiency and variability. Six single-spin methods of PRP production from whole blood were analyzed. The primary measures were mean yield and standard de...
Source: Platelets - September 11, 2019 Category: Hematology Tags: Platelets Source Type: research

Correction of Severe Myelofibrosis, Impaired Platelet Functions and Abnormalities in a Patient with Gray Platelet Syndrome Successfully Treated by Stem Cell Transplantation.
We report a male GPS patient with severe pancytopenia, splenomegaly and a secondary myelofibrosis needing red blood cells transfusion. He received an HSCT from a 10/10 matched HLA-unrelated donor after a myeloablative conditioning regimen. Transfusion independence occurred at day+21, with a documented neutrophil engraftment. At day+ 180, we added ruxolitinib to cyclosporine and steroids for a moderate chronic graft versus host disease (GVHD) and persistent splenomegaly. At day+240 GVHD was controlled and splenomegaly reduced. Complete donor chimesrism was documented in blood and marrow and platelets functions and morpholog...
Source: Platelets - September 11, 2019 Category: Hematology Tags: Platelets Source Type: research

Use of glycoprotein IIb/IIIa antagonists to prevent stent thrombosis in morphine-treated patients with ST-elevation myocardial infarction.
In conclusion, routine GPI use in morphine-treated STEMI patients undergoing PPCI appears to protect against stent thrombosis. Large-scale studies are needed to establish the overall risk-benefit of GPI therapy in morphine-treated PPCI patients and to assess alternative strategies for preventing acute stent thrombosis in these patients. PMID: 31502505 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - September 11, 2019 Category: Hematology Tags: Platelets Source Type: research

Percutaneous coronary intervention in patients with essential thrombocythemia: case reports and literature review.
In conclusion, PCI is an effective method of revascularization in patients with ET; but it may be associated with a higher rate of complications including stent thrombus and restenosis. PMID: 31502506 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - September 11, 2019 Category: Hematology Tags: Platelets Source Type: research

Inhibition of platelet activation using vitamins.
Authors: Kobzar G Abstract Some vitamins have beneficial effects on cardiovascular diseases, normalizing platelet function and preventing their excess activation. Anti-platelet vitamins can act directly through inhibitory biochemical pathways in platelets or indirectly by preventing damage to the endothelium or low-density lipoprotein from oxidation. As a rule, each vitamin alone is a weak inhibitor of platelet aggregation. However, in combination, they may act synergistically or enhance the effects of endogenous anti-platelet compounds, such as prostacyclin or nitric oxide, and appear to have a sufficient anti-thr...
Source: Platelets - August 21, 2019 Category: Hematology Tags: Platelets Source Type: research

Fondaparinux cross-reactivity in heparin-induced thrombocytopenia successfully treated with high-dose intravenous immunoglobulin and rivaroxaban.
Authors: Manji F, Warkentin TE, Sheppard JI, Lee A Abstract HIT, a prothrombotic disorder caused by heparin-dependent antibodies, is often treated with fondaparinux, usually with good outcomes. A 70-year-old female developed severe HIT (platelet count, 25 × 109/L) post-glioblastoma resection during heparin thromboprophylaxis, complicated by disseminated intravascular coagulation (DIC) and symptomatic lower-limb deep-vein thrombosis (DVT). Despite therapeutic-dose fondaparinux, thrombocytopenia/hypofibrinogenemia persisted, with new symptomatic catheter-associated upper-extremity DVT. This clinical picture cou...
Source: Platelets - August 11, 2019 Category: Hematology Tags: Platelets Source Type: research

Platelet dysfunction caused by a novel thromboxane A2 receptor mutation and congenital thrombocytopenia in a case of mild bleeding.
In conclusion, the bleeding phenotype of the index patient can be explained by a diminished platelet function caused by the TBXA2R*c.908T>C mutation inherited from the mother and a mild thrombocytopenia with unknown molecular basis that is inherited from the father. PMID: 31389738 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - August 9, 2019 Category: Hematology Tags: Platelets Source Type: research

The association of P2Y12 inhibitors with pro-coagulatory extracellular vesicles and microRNAs in stable coronary artery disease.
Authors: Haller PM, Stojkovic S, Piackova E, Andric T, Wisgrill L, Spittler A, Wojta J, Huber K, Jäger B Abstract Extracellular vesicles (EV) act as a cellular communication tool by carrying lipids, proteins and micro RNA (miR) between cells, thereby playing a pivotal role in thromboembolic processes. The effect of P2Y12 inhibitors on pro-coagulatory, phosphatidylserine (PS)-expressing EV has been investigated previously, but only in vitro or during confounding clinical conditions, such as acute coronary syndrome. Hence, we enrolled 62 consecutive patients 12 month after percutaneous coronary intervention and ...
Source: Platelets - August 9, 2019 Category: Hematology Tags: Platelets Source Type: research

Plasma levels of the soluble form of the Fc γRIIa receptor vary with receptor polymorphisms and are elevated in rheumatoid arthritis.
This study aimed to evaluate mechanisms of FcγRIIa release compared to GPVI release from platelets, and examine whether genetic polymorphisms at positions 27 and 131 within FcγRIIa correlate with platelet FcγRIIa stability and function. Enzyme-linked immunosorbent assays (ELISAs) were used to measure plasma sFcγRIIa and sGPVI levels. FcγRIIa genotype at positions 27 and 131 was evaluated. sFcγRIIa levels were not significantly different between non-131HH and 131HH but were significantly lower in 27W than non-27W. Treatment of platelets with aggregated immunoglobulin (Ig) G induced releas...
Source: Platelets - August 7, 2019 Category: Hematology Tags: Platelets Source Type: research

SLFN14 gene mutations associated with bleeding.
Authors: Stapley RJ, Pisareva VP, Pisarev AV, Morgan NV PMID: 31378119 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - August 7, 2019 Category: Hematology Tags: Platelets Source Type: research

Both the platelet count and the platelet: lymphocyte ratio are not increased in nascent metabolic syndrome.
Authors: Jialal G, Adams-Huet B, Jialal I PMID: 31373253 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - August 4, 2019 Category: Hematology Tags: Platelets Source Type: research

Experimental thrombocytopenia does not affect acute kidney injury 24 hours after renal ischemia reperfusion in mice.
Authors: Jansen MPB, Huisman A, Claessen N, Florquin S, Roelofs JJTH Abstract The pathophysiology of renal ischemia/reperfusion (I/R) injury is characterized by excessive activation of inflammation and coagulation processes followed by abnormal renal tissue repair, resulting in renal injury and function loss. Platelets are important actors in these processes, however to what extent platelets contribute to the pathophysiology of renal I/R injury still needs to be elucidated. In the current study, we treated wild-type mice with a platelet depleting antibody, which caused thrombocytopenia. We then investigated the rol...
Source: Platelets - August 2, 2019 Category: Hematology Tags: Platelets Source Type: research

Antiplatelet activity of deferiprone through cyclooxygenase-1 inhibition.
Authors: Tran NT, Akkawat B, Morales NP, Rojnuckarin P, Luechapudiporn R Abstract Thalassemia patients are susceptible to both iron overload and thromboembolism. Deferiprone is an iron chelator that shows an antiplatelet activity and thus may alleviate platelet hyperactivation in thalassemia. Therefore, this study aimed to characterize the inhibitory effects and mechanisms of deferiprone on normal human platelets. The results illustrated that deferiprone inhibited platelet aggregation at the iron chelating concentrations (0.08-0.25 mmol/l). Deferiprone inhibited human platelet aggregation stimulated by arachid...
Source: Platelets - August 2, 2019 Category: Hematology Tags: Platelets Source Type: research

Long-term and short-term duration of thienopyridine therapy after coronary stenting in patients with chronic kidney disease a meta-analysis of literature studies.
Authors: Wu Y, Song Y, Pan Y, Gong Y, Zhou Y Abstract The study aimed to compare the efficacy and safety outcome associated with a short and a prolonged duration of thienopyridine therapy in patients with chronic kidney disease (CKD) after coronary stenting. We systematically searched PubMed, EMBASE and the Cochrane Library from their inception to 1 January 2019 for studies comparing short and prolonged thienopyridine therapy in patients with CKD. Ischemic and bleeding events were considered as the clinical endpoints in this analysis. Odds Ratios (OR) with 95% confidence intervals (CIs) were used as estimates of ef...
Source: Platelets - August 1, 2019 Category: Hematology Tags: Platelets Source Type: research

Effect of leukocytes included in platelet concentrates on cell behaviour.
Authors: Puidokas T, Kubilius M, Stumbras A, Juodzbalys G Abstract This literature review's aim is to evaluate the function of white blood cells (WBCs) and to conclude whether leukocyte's inclusion in platelet concentrates promotes or inhibits tissue regeneration. An electronic literature search was performed on MEDLINE through its online site (PubMed) and on the EMBASE database for articles published between 1986 and 2019. Consecutive screenings at the title/abstract and full-text levels were performed. All in vitro studies that reported the comparison of leukocyte-rich and leukocyte-poor platelet concentrates wer...
Source: Platelets - July 26, 2019 Category: Hematology Tags: Platelets Source Type: research

Preeclampsia and platelets activation.
Authors: Ponzetto A, Turvani G PMID: 31340711 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - July 26, 2019 Category: Hematology Tags: Platelets Source Type: research

Validated model of platelet slip at stenosis and device surfaces.
The objective of this study was to explore whether microspheres-modeling platelets-slip at different vascular and device surfaces in an acrylic scaled-up model coronary artery. The microspheres (3.12 µm diameter) were suspended in a transparent glycerol/water experimental fluid, which flowed continuously at Reynolds numbers typical of coronary flow (200-400) through the model artery. We placed a series of axisymmetric acrylic stenoses (cross-sectional area reduction [CSAr], 20-90%) into the model artery, both without and with a central cylinder present (modeling a percutaneous interventional guide wire, and with a sc...
Source: Platelets - July 19, 2019 Category: Hematology Tags: Platelets Source Type: research

Managing ITP and thrombocytopenia in pregnancy.
Authors: Eslick R, McLintock C Abstract Around 1 in 10 pregnant women will develop thrombocytopenia during an otherwise unremarkable pregnancy. While the most frequent cause is gestational thrombocytopenia, a benign clinical entity which typically induces a mild platelet fall in late pregnancy, a number of important pregnancy-specific causes must be excluded, particularly pre-eclampsia and its severe form hemolysis with elevated liver enzymes and low platelets (HELLP). For women who do not have an identifiable pregnancy-related cause of thrombocytopenia, an underlying medical condition should be considered. The mos...
Source: Platelets - July 13, 2019 Category: Hematology Tags: Platelets Source Type: research

Increased microvesicle-associated thrombin generation in patients with immune thrombocytopenia after initiation of thrombopoietin receptor agonists.
In conclusion, TPO-RAs increase phospholipid-dependent MV-associated thrombin generation in ITP patients. This could contribute to or exacerbate a pre-existing hypercoagulable state. Phospholipid-dependent thrombin generation generated by isolated MVs, or measured directly in plasma, may be potential tools that could help in the risk-assessment of future thromboembolic events in ITP patients, both before and after initiation of TPO-RA-treatment. PMID: 31280643 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - July 10, 2019 Category: Hematology Tags: Platelets Source Type: research

The incidence and clinical burden of immune thrombocytopenia in pediatric patients in the United States.
Authors: Shaw J, Kilpatrick K, Eisen M, Tarantino M Abstract Immune thrombocytopenia (ITP) is the most common bleeding disorder diagnosed in children. Characterized by low platelet counts, it leads to reduced clotting abilities and an increased tendency to bleed. The disorder in children is often self-limiting. However, approximately 25% of children develop persistent or chronic ITP, and bleeding associated with thrombocytopenia can be life-threatening. The current incidence of ITP in the US and the characterization of the illness among children being managed in routine clinical practice are sparsely reported. This...
Source: Platelets - July 6, 2019 Category: Hematology Tags: Platelets Source Type: research

Second-line and beyond: treatment options for primary persistent and chronic immune thrombocytopenia.
Authors: Deshayes S, Godeau B Abstract Less than 40% of patients with newly diagnosed adult immune thrombocytopenia will show spontaneous recovery within 12 months. Therefore, second-line treatments are frequently used to maintain a hemostatic platelet count or at best to cure the disease, with as few adverse effects as possible. Nevertheless, we lack head-to-head comparison studies of the different available treatments. Moreover, physicians have no robust predictors of response to guide decision-making on an individual basis. Therefore, there is no consensus, and decisions when to treat and with which drug must be...
Source: Platelets - July 6, 2019 Category: Hematology Tags: Platelets Source Type: research

Platelet count in men with a habit of alcohol drinking.
Authors: Wakabayashi I Abstract Alcohol inhibits platelet function, and platelet count is often reduced in individuals with alcohol use disorder. However, the relation of habitual alcohol drinking with platelet count in a general population remains to be determined. The participants were 6508 men (30 ~ 69 years old) who had received annual health checkup examinations, and most of them (98.6%) were nondrinkers or drinkers with an average ethanol intake of less than 66 g per day. Relationships of platelet count with alcohol intake were investigated by using analysis of covariance and multivariate linear and logistic ...
Source: Platelets - July 5, 2019 Category: Hematology Tags: Platelets Source Type: research

How I approach new onset thrombocytopenia.
Authors: Swain F, Bird R Abstract Thrombocytopenia is a common reason for referral to hematologists in community and hospital practice. A broad differential diagnosis, combined with the potentially life-threatening nature of some presentations necessitates a rapid evaluation of the situation and potential need for emergency intervention; followed by further comprehensive investigation to confirm the diagnosis and institution of longer term management. This review offers an approach to the initial assessment, diagnosis, and referral. We then highlight aspects of the clinical history, examination and laboratory inves...
Source: Platelets - July 5, 2019 Category: Hematology Tags: Platelets Source Type: research

Acquired platelet dysfunction and overproduction of platelet cyclic AMP in two patients with myeloid malignancies.
In conclusion, these are the first patients with myeloid malignancies associated with acquired severe platelet dysfunction and overproduction of cAMP. PMID: 31240986 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - June 28, 2019 Category: Hematology Tags: Platelets Source Type: research

Platelet microRNA expression and association with platelet maturity and function in patients with essential thrombocythemia.
In this study, we included 22 ET patients and 19 healthy controls to investigate the expression of 12 platelet miRNAs previously reported to be dysregulated in ET. Further, we investigated the correlation between the expression of selected miRNAs and platelet maturity and platelet function. Total RNA was isolated from platelets, and expression analyses were performed using TaqMan quantitative PCR (qPCR). Mean platelet volume (MPV) and immature platelet count and -fraction (IPC and IPF) were measured using the Sysmex XE-5000 automated haematology system. Platelet function was investigated by multiple electrode aggregometry ...
Source: Platelets - June 28, 2019 Category: Hematology Tags: Platelets Source Type: research

De novo variant in tyrosine kinase SRC causes thrombocytopenia: case report of a second family.
This study strengthens our previous finding that hyperactive SRC kinase results in mild platelet dysfunction and thrombocytopenia with hypogranular platelets and further expands the clinical description of this syndrome to improve early recognition. PMID: 31204551 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - June 19, 2019 Category: Hematology Tags: Platelets Source Type: research

The efficacy and safety of low-dose rituximab in immune thrombocytopenia: a systematic review and meta-analysis.
Authors: Li Y, Shi Y, He Z, Chen Q, Liu Z, Yu L, Wang C Abstract Rituximab has been frequently used as a second-line treatment for patients with immune thrombocytopenia (ITP). Recently, several studies have proposed low-dose (100 mg or 100mg/m2 per week for 4 weeks) rituximab instead of the standard dose of 375mg/m2 per week for 4 weeks to treat ITP patients. The aim of this review was to systematically evaluate the efficacy and safety of low-dose rituximab for patients with ITP. Pubmed, Web of Science, Cochrane Library and Embase were searched to identify the clinical studies published in full text or abstract tha...
Source: Platelets - June 6, 2019 Category: Hematology Tags: Platelets Source Type: research

Thrombin-induced platelet aggregation -effect of dabigatran using automated platelet aggregometry.
Authors: Shimizu M, Natori T, Tsuda K, Yoshida M, Kamada A, Oi K, Ishigaku Y, Oura K, Narumi S, Yamamoto M, Terayama Y Abstract Dabigatran, a direct oral thrombin inhibitor, has two therapeutic effects: anticoagulation; and antiplatelet activity. In the clinical field, evaluation of the effect of dabigatran on thrombin-induced platelet aggregation is difficult because of fibrin clot formation and platelet aggregation. The aim of this study was to establish a new platelet aggregation method and to investigate the effects of dabigatran on thrombin-induced platelet aggregation. Platelet aggregation with thrombin was p...
Source: Platelets - June 6, 2019 Category: Hematology Tags: Platelets Source Type: research

Detection of tissue factor in platelets: why is it so troublesome?
This article discusses notable reports and possible reasons for erroneous detection of platelet TF antigen and activity including artificially hyper-stimulated platelets, suboptimal purity of cell preparations, flaws in study design and/or choice of reagents. PMID: 31146606 [PubMed - as supplied by publisher] (Source: Platelets)
Source: Platelets - May 31, 2019 Category: Hematology Tags: Platelets Source Type: research