Novel pathogenic variants in Tubulin Tyrosine Like 5 ( < em > TTLL5) < /em > associated with cone-dominant retinal dystrophies and an abnormal optical coherence tomography phenotype
CONCLUSION: Based on the protein domain location of novel TTLL5 variants and the localization of TTLL5 to the connecting cilium, we conclude that the CD/CRD disease phenotype is characterized as a ciliopathy caused by protein tracking dysfunction. This initially affects cone photoreceptors, where photoreceptor cilia express a high level of TTLL5, but extends to rod photoreceptors over time. Fundus photography correlated with SD-OCT imaging suggests that the macular sheen characteristically seen with TTLL5 mutations derives from the photoreceptor's outer segments at the posterior pole.PMID:38264610 | PMC:PMC10805330 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Olubayo U Kolawole Cheryl Y Gregory-Evans Riyaz Bikoo Albert Z Huang Kevin Gregory-Evans Source Type: research
Sulforaphane inhibits TGF- β-induced fibrogenesis and inflammation in human Tenon's fibroblasts
CONCLUSIONS: SFN inhibits HTF contractility, differentiation into myofibroblasts, and inflammation caused by TGF-β. These effects are mediated by both classic and non-classic signaling pathways. Our results indicate that SFN has potent anti-fibrotic and anti-inflammatory effects in HTFs and is a potential candidate for subconjunctival fibrosis therapy.PMID:38264611 | PMC:PMC10805336 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Yang Liu Yangbin Huang Zihan Guo Chengcheng Yang Yunzepeng Li Binhui Li Ye Liu Hui Zheng Source Type: research
Therapeutic e ffects of a novel venom abstract (ZK002) solution in an alkali-burned corneal wound-healing model
CONCLUSIONS: ZK002 decreased the fibrotic reaction and prevented neovascularization in the cornea after an alkali burn. Therefore, the novel ophthalmic solution ZK002 could be a potentially promising therapeutic clinical treatment for corneal wound healing.PMID:38264612 | PMC:PMC10805332 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Wen-Yan Peng Fei Wang Shuang-Jian Yang Qin-Yan Sun Heng-Shen Zhou Xiaoyi Li Zheng-Xuan Jiang Shi-You Zhou Source Type: research
Suppressor of cytokine signaling 3-derived peptide as a therapeutic for inflammatory and oxidative stress-induced damage to the retina
CONCLUSIONS: R9-SOCS3-KIR blocks the induction of inflammatory signaling in cell culture and reduces retinal damage in a widely used RPE/retinal oxidative injury model. As this peptide can be administered through corneal instillation, this treatment may offer a convenient way to slow down the progression of ocular diseases arising from inflammation and chronic oxidative stress.PMID:38264613 | PMC:PMC10805335 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Chulbul M Ahmed Anil P Patel Howard M Johnson Cristhian J Ildefonso Alfred S Lewin Source Type: research
Evaluation of the effects of latanoprost and benzalkonium chloride on the cell viability and nonpolar lipid profile produced by human meibomian gland epithelial cells in culture
CONCLUSIONS: The use of latanoprost and BAK in glaucoma may alter the viability of the meibomian glands and their lipid expression in vivo. Sublethal concentrations of BAK appear to modulate meibum lipid expression, particularly in relation to sterol biosynthesis. Non-preserved latanoprost had less cytotoxicity at lower doses and fewer lipidomic effects compared to BAK, further strengthening the argument in favor of BAK-free pharmaceutical preparations.PMID:38264609 | PMC:PMC10805331 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Jillian F Ziemanski Landon Wilson Stephen Barnes Kelly K Nichols Source Type: research
Novel pathogenic variants in Tubulin Tyrosine Like 5 ( < em > TTLL5) < /em > associated with cone-dominant retinal dystrophies and an abnormal optical coherence tomography phenotype
CONCLUSION: Based on the protein domain location of novel TTLL5 variants and the localization of TTLL5 to the connecting cilium, we conclude that the CD/CRD disease phenotype is characterized as a ciliopathy caused by protein tracking dysfunction. This initially affects cone photoreceptors, where photoreceptor cilia express a high level of TTLL5, but extends to rod photoreceptors over time. Fundus photography correlated with SD-OCT imaging suggests that the macular sheen characteristically seen with TTLL5 mutations derives from the photoreceptor's outer segments at the posterior pole.PMID:38264610 | PMC:PMC10805330 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Olubayo U Kolawole Cheryl Y Gregory-Evans Riyaz Bikoo Albert Z Huang Kevin Gregory-Evans Source Type: research
Sulforaphane inhibits TGF- β-induced fibrogenesis and inflammation in human Tenon's fibroblasts
CONCLUSIONS: SFN inhibits HTF contractility, differentiation into myofibroblasts, and inflammation caused by TGF-β. These effects are mediated by both classic and non-classic signaling pathways. Our results indicate that SFN has potent anti-fibrotic and anti-inflammatory effects in HTFs and is a potential candidate for subconjunctival fibrosis therapy.PMID:38264611 | PMC:PMC10805336 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Yang Liu Yangbin Huang Zihan Guo Chengcheng Yang Yunzepeng Li Binhui Li Ye Liu Hui Zheng Source Type: research
Therapeutic e ffects of a novel venom abstract (ZK002) solution in an alkali-burned corneal wound-healing model
CONCLUSIONS: ZK002 decreased the fibrotic reaction and prevented neovascularization in the cornea after an alkali burn. Therefore, the novel ophthalmic solution ZK002 could be a potentially promising therapeutic clinical treatment for corneal wound healing.PMID:38264612 | PMC:PMC10805332 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Wen-Yan Peng Fei Wang Shuang-Jian Yang Qin-Yan Sun Heng-Shen Zhou Xiaoyi Li Zheng-Xuan Jiang Shi-You Zhou Source Type: research
Suppressor of cytokine signaling 3-derived peptide as a therapeutic for inflammatory and oxidative stress-induced damage to the retina
CONCLUSIONS: R9-SOCS3-KIR blocks the induction of inflammatory signaling in cell culture and reduces retinal damage in a widely used RPE/retinal oxidative injury model. As this peptide can be administered through corneal instillation, this treatment may offer a convenient way to slow down the progression of ocular diseases arising from inflammation and chronic oxidative stress.PMID:38264613 | PMC:PMC10805335 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Chulbul M Ahmed Anil P Patel Howard M Johnson Cristhian J Ildefonso Alfred S Lewin Source Type: research
< em > GPR143 < /em > mutations in an X-linked infantile nystagmus syndrome cohort in Southeast China
CONCLUSIONS: We discovered seven novel mutations and four previously reported mutations of GPR143 in a cohort of families with X-linked INS and enlarged the Chinese genetic spectrum of INS. These findings offer new insights for developing genetic screening strategies and shed light on the importance of conducting genetic analysis in confirming the clinical diagnosis in unresolved patients and atypical phenotypes.PMID:38222445 | PMC:PMC10784212 (Source: Molecular Vision)
Source: Molecular Vision - January 15, 2024 Category: Molecular Biology Authors: Jingling Xu Yihan Zheng Lulu Cheng Huihui Sun Xinping Yu Feng Gu E Song Source Type: research
Investigation of the antioxidant effect of Chrysin in an experimental cataract model created in chick embryos
CONCLUSIONS: We think that anti-cataract effect of crhysin may be due to the antioxidant and antiapoptotic properties of chrysin. However, more research is needed to clarify the anti-cataract effects of chrysin.PMID:38222446 | PMC:PMC10784222 (Source: Molecular Vision)
Source: Molecular Vision - January 15, 2024 Category: Molecular Biology Authors: G ülan Albaş Kurt Tolga Ertekin Emre Atay Abd ülkadir Bilir Halit Bu ğra Koca Esra Aslan Alperen Sar ıtaş Source Type: research
Oxidative stress-induced temporal activation of ERK1/2 phosphorylates coreceptor of Wnt/ β-catenin for myofibroblast formation in human lens epithelial cells
CONCLUSIONS: ERK1/2 signaling plays a crucial role in the moderate level of oxidative stress-induced EMT in LECs. Pharmacologically blocking ERK1/2 signaling significantly inhibited LEC proliferation and EMT. Mechanistically, ERK1/2 signaling regulated Wnt/β-catenin cascade by phosphorylating Wnt coreceptor LRP6 at Ser 1490 in the plasma membrane. These results shed light on a potential molecular switch of ERK1/2 and Wnt/β-catenin crosstalk underlying the development of PCO.PMID:38222447 | PMC:PMC10784218 (Source: Molecular Vision)
Source: Molecular Vision - January 15, 2024 Category: Molecular Biology Authors: Zaoxia Guo Xiaopan Ma Xi Chen Rui Xue Zhang Hong Yan Source Type: research
Changes in glutamate and glutamine distributions in the retinas of cystine/glutamate antiporter knockout mice
CONCLUSION: Loss of xCT function results in glutamate metabolic disruption through the accumulation of glutamate in photoreceptors and a reduced uptake of glutamate by Müller cells, which in turn decreases glutamine production. These findings support the idea that xCT plays a role in the presynaptic metabolism of photoreceptors and postsynaptic levels of glutamate and derived neurotransmitters in the retina.PMID:38222448 | PMC:PMC10784226 (Source: Molecular Vision)
Source: Molecular Vision - January 15, 2024 Category: Molecular Biology Authors: Luis J Knight Renita M Martis Paul J Donaldson Monica L Acosta Julie C Lim Source Type: research
Evaluation of the Algerbrush II rotating burr as a tool for inducing ocular surface failure in a mouse model
CONCLUSIONS: Our findings suggest that the duration of injury is an important factor influencing the severity of LSCD in a murine model of injury. A 1-mm rotating burr was found to be more effective for keratectomy and pigment release, whereas a 0.5-mm burr was more suitable for corneal epithelial debridement.PMID:38222449 | PMC:PMC10784216 (Source: Molecular Vision)
Source: Molecular Vision - January 15, 2024 Category: Molecular Biology Authors: Athar Shadmani Trent Jarin Xiang Qi Meng Yugendran Rajaendran Salih Uzun Albert Y Wu Source Type: research
Addressing bias in manual segmentation of spheroid sprouting assays with U-Net
CONCLUSIONS: This method may prove useful for verifying positive findings in angiogenesis experiments with a manual analysis pipeline when full investigator masking has been neglected or is not feasible.PMID:38222450 | PMC:PMC10784213 (Source: Molecular Vision)
Source: Molecular Vision - January 15, 2024 Category: Molecular Biology Authors: Julian Rapp Daniel B öhringer G ünther Schlunck Hansj ürgen Agostini Thomas Reinhard Felicitas Bucher Source Type: research
