Evaluation of the effects of latanoprost and benzalkonium chloride on the cell viability and nonpolar lipid profile produced by human meibomian gland epithelial cells in culture
CONCLUSIONS: The use of latanoprost and BAK in glaucoma may alter the viability of the meibomian glands and their lipid expression in vivo. Sublethal concentrations of BAK appear to modulate meibum lipid expression, particularly in relation to sterol biosynthesis. Non-preserved latanoprost had less cytotoxicity at lower doses and fewer lipidomic effects compared to BAK, further strengthening the argument in favor of BAK-free pharmaceutical preparations.PMID:38264609 | PMC:PMC10805331 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Jillian F Ziemanski Landon Wilson Stephen Barnes Kelly K Nichols Source Type: research

Novel pathogenic variants in Tubulin Tyrosine Like 5 ( < em > TTLL5) < /em > associated with cone-dominant retinal dystrophies and an abnormal optical coherence tomography phenotype
CONCLUSION: Based on the protein domain location of novel TTLL5 variants and the localization of TTLL5 to the connecting cilium, we conclude that the CD/CRD disease phenotype is characterized as a ciliopathy caused by protein tracking dysfunction. This initially affects cone photoreceptors, where photoreceptor cilia express a high level of TTLL5, but extends to rod photoreceptors over time. Fundus photography correlated with SD-OCT imaging suggests that the macular sheen characteristically seen with TTLL5 mutations derives from the photoreceptor's outer segments at the posterior pole.PMID:38264610 | PMC:PMC10805330 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Olubayo U Kolawole Cheryl Y Gregory-Evans Riyaz Bikoo Albert Z Huang Kevin Gregory-Evans Source Type: research

Sulforaphane inhibits TGF- β-induced fibrogenesis and inflammation in human Tenon's fibroblasts
CONCLUSIONS: SFN inhibits HTF contractility, differentiation into myofibroblasts, and inflammation caused by TGF-β. These effects are mediated by both classic and non-classic signaling pathways. Our results indicate that SFN has potent anti-fibrotic and anti-inflammatory effects in HTFs and is a potential candidate for subconjunctival fibrosis therapy.PMID:38264611 | PMC:PMC10805336 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Yang Liu Yangbin Huang Zihan Guo Chengcheng Yang Yunzepeng Li Binhui Li Ye Liu Hui Zheng Source Type: research

Therapeutic e ffects of a novel venom abstract (ZK002) solution in an alkali-burned corneal wound-healing model
CONCLUSIONS: ZK002 decreased the fibrotic reaction and prevented neovascularization in the cornea after an alkali burn. Therefore, the novel ophthalmic solution ZK002 could be a potentially promising therapeutic clinical treatment for corneal wound healing.PMID:38264612 | PMC:PMC10805332 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Wen-Yan Peng Fei Wang Shuang-Jian Yang Qin-Yan Sun Heng-Shen Zhou Xiaoyi Li Zheng-Xuan Jiang Shi-You Zhou Source Type: research

Suppressor of cytokine signaling 3-derived peptide as a therapeutic for inflammatory and oxidative stress-induced damage to the retina
CONCLUSIONS: R9-SOCS3-KIR blocks the induction of inflammatory signaling in cell culture and reduces retinal damage in a widely used RPE/retinal oxidative injury model. As this peptide can be administered through corneal instillation, this treatment may offer a convenient way to slow down the progression of ocular diseases arising from inflammation and chronic oxidative stress.PMID:38264613 | PMC:PMC10805335 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Chulbul M Ahmed Anil P Patel Howard M Johnson Cristhian J Ildefonso Alfred S Lewin Source Type: research

Evaluation of the effects of latanoprost and benzalkonium chloride on the cell viability and nonpolar lipid profile produced by human meibomian gland epithelial cells in culture
CONCLUSIONS: The use of latanoprost and BAK in glaucoma may alter the viability of the meibomian glands and their lipid expression in vivo. Sublethal concentrations of BAK appear to modulate meibum lipid expression, particularly in relation to sterol biosynthesis. Non-preserved latanoprost had less cytotoxicity at lower doses and fewer lipidomic effects compared to BAK, further strengthening the argument in favor of BAK-free pharmaceutical preparations.PMID:38264609 | PMC:PMC10805331 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Jillian F Ziemanski Landon Wilson Stephen Barnes Kelly K Nichols Source Type: research

Novel pathogenic variants in Tubulin Tyrosine Like 5 ( < em > TTLL5) < /em > associated with cone-dominant retinal dystrophies and an abnormal optical coherence tomography phenotype
CONCLUSION: Based on the protein domain location of novel TTLL5 variants and the localization of TTLL5 to the connecting cilium, we conclude that the CD/CRD disease phenotype is characterized as a ciliopathy caused by protein tracking dysfunction. This initially affects cone photoreceptors, where photoreceptor cilia express a high level of TTLL5, but extends to rod photoreceptors over time. Fundus photography correlated with SD-OCT imaging suggests that the macular sheen characteristically seen with TTLL5 mutations derives from the photoreceptor's outer segments at the posterior pole.PMID:38264610 | PMC:PMC10805330 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Olubayo U Kolawole Cheryl Y Gregory-Evans Riyaz Bikoo Albert Z Huang Kevin Gregory-Evans Source Type: research

Sulforaphane inhibits TGF- β-induced fibrogenesis and inflammation in human Tenon's fibroblasts
CONCLUSIONS: SFN inhibits HTF contractility, differentiation into myofibroblasts, and inflammation caused by TGF-β. These effects are mediated by both classic and non-classic signaling pathways. Our results indicate that SFN has potent anti-fibrotic and anti-inflammatory effects in HTFs and is a potential candidate for subconjunctival fibrosis therapy.PMID:38264611 | PMC:PMC10805336 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Yang Liu Yangbin Huang Zihan Guo Chengcheng Yang Yunzepeng Li Binhui Li Ye Liu Hui Zheng Source Type: research

Therapeutic e ffects of a novel venom abstract (ZK002) solution in an alkali-burned corneal wound-healing model
CONCLUSIONS: ZK002 decreased the fibrotic reaction and prevented neovascularization in the cornea after an alkali burn. Therefore, the novel ophthalmic solution ZK002 could be a potentially promising therapeutic clinical treatment for corneal wound healing.PMID:38264612 | PMC:PMC10805332 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Wen-Yan Peng Fei Wang Shuang-Jian Yang Qin-Yan Sun Heng-Shen Zhou Xiaoyi Li Zheng-Xuan Jiang Shi-You Zhou Source Type: research

Suppressor of cytokine signaling 3-derived peptide as a therapeutic for inflammatory and oxidative stress-induced damage to the retina
CONCLUSIONS: R9-SOCS3-KIR blocks the induction of inflammatory signaling in cell culture and reduces retinal damage in a widely used RPE/retinal oxidative injury model. As this peptide can be administered through corneal instillation, this treatment may offer a convenient way to slow down the progression of ocular diseases arising from inflammation and chronic oxidative stress.PMID:38264613 | PMC:PMC10805335 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Chulbul M Ahmed Anil P Patel Howard M Johnson Cristhian J Ildefonso Alfred S Lewin Source Type: research

Evaluation of the effects of latanoprost and benzalkonium chloride on the cell viability and nonpolar lipid profile produced by human meibomian gland epithelial cells in culture
CONCLUSIONS: The use of latanoprost and BAK in glaucoma may alter the viability of the meibomian glands and their lipid expression in vivo. Sublethal concentrations of BAK appear to modulate meibum lipid expression, particularly in relation to sterol biosynthesis. Non-preserved latanoprost had less cytotoxicity at lower doses and fewer lipidomic effects compared to BAK, further strengthening the argument in favor of BAK-free pharmaceutical preparations.PMID:38264609 | PMC:PMC10805331 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Jillian F Ziemanski Landon Wilson Stephen Barnes Kelly K Nichols Source Type: research

Novel pathogenic variants in Tubulin Tyrosine Like 5 ( < em > TTLL5) < /em > associated with cone-dominant retinal dystrophies and an abnormal optical coherence tomography phenotype
CONCLUSION: Based on the protein domain location of novel TTLL5 variants and the localization of TTLL5 to the connecting cilium, we conclude that the CD/CRD disease phenotype is characterized as a ciliopathy caused by protein tracking dysfunction. This initially affects cone photoreceptors, where photoreceptor cilia express a high level of TTLL5, but extends to rod photoreceptors over time. Fundus photography correlated with SD-OCT imaging suggests that the macular sheen characteristically seen with TTLL5 mutations derives from the photoreceptor's outer segments at the posterior pole.PMID:38264610 | PMC:PMC10805330 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Olubayo U Kolawole Cheryl Y Gregory-Evans Riyaz Bikoo Albert Z Huang Kevin Gregory-Evans Source Type: research

Sulforaphane inhibits TGF- β-induced fibrogenesis and inflammation in human Tenon's fibroblasts
CONCLUSIONS: SFN inhibits HTF contractility, differentiation into myofibroblasts, and inflammation caused by TGF-β. These effects are mediated by both classic and non-classic signaling pathways. Our results indicate that SFN has potent anti-fibrotic and anti-inflammatory effects in HTFs and is a potential candidate for subconjunctival fibrosis therapy.PMID:38264611 | PMC:PMC10805336 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Yang Liu Yangbin Huang Zihan Guo Chengcheng Yang Yunzepeng Li Binhui Li Ye Liu Hui Zheng Source Type: research

Therapeutic e ffects of a novel venom abstract (ZK002) solution in an alkali-burned corneal wound-healing model
CONCLUSIONS: ZK002 decreased the fibrotic reaction and prevented neovascularization in the cornea after an alkali burn. Therefore, the novel ophthalmic solution ZK002 could be a potentially promising therapeutic clinical treatment for corneal wound healing.PMID:38264612 | PMC:PMC10805332 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Wen-Yan Peng Fei Wang Shuang-Jian Yang Qin-Yan Sun Heng-Shen Zhou Xiaoyi Li Zheng-Xuan Jiang Shi-You Zhou Source Type: research

Suppressor of cytokine signaling 3-derived peptide as a therapeutic for inflammatory and oxidative stress-induced damage to the retina
CONCLUSIONS: R9-SOCS3-KIR blocks the induction of inflammatory signaling in cell culture and reduces retinal damage in a widely used RPE/retinal oxidative injury model. As this peptide can be administered through corneal instillation, this treatment may offer a convenient way to slow down the progression of ocular diseases arising from inflammation and chronic oxidative stress.PMID:38264613 | PMC:PMC10805335 (Source: Molecular Vision)
Source: Molecular Vision - January 24, 2024 Category: Molecular Biology Authors: Chulbul M Ahmed Anil P Patel Howard M Johnson Cristhian J Ildefonso Alfred S Lewin Source Type: research