Reply: Methodological considerations in measuring different AMH splice forms using ELISA: validity of proAMH ELISA
(Source: Molecular Human Reproduction)
Source: Molecular Human Reproduction - April 26, 2016 Category: Molecular Biology Authors: Mamsen, L. S., Munthe-Fog, L., Petersen, T. S., Jeppesen, J. V., Mollgard, K., Grondahl, M. L., Larsen, A., Ernst, E., Oxvig, C., Kumar, A., Kalra, B., Andersen, C. Y. Tags: Letters to the Editor Source Type: research
Methodological considerations in measuring different AMH cleavage forms using ELISA: validity of proAMH ELISA
(Source: Molecular Human Reproduction)
Source: Molecular Human Reproduction - April 26, 2016 Category: Molecular Biology Authors: McLennan, I. S., Pankhurst, M. W. Tags: Letters to the Editor Source Type: research
Whole exome sequencing in recurrent early pregnancy loss
STUDY HYPOTHESIS
Exome sequencing can identify genetic causes of idiopathic recurrent pregnancy loss (RPL).
STUDY FINDING
We identified compound heterozygous deleterious mutations affecting DYNC2H1 and ALOX15 in two out of four families with RPL. Both genes have a role in early development. Bioinformatics analysis of all genes with rare and putatively pathogenic mutations in miscarriages and couples showed enrichment in pathways relevant to pregnancy loss, including the complement and coagulation cascades pathways.
WHAT IS KNOWN ALREADY
Next generation sequencing (NGS) is increasingly being used to identify known and nov...
Source: Molecular Human Reproduction - April 26, 2016 Category: Molecular Biology Authors: Qiao, Y., Wen, J., Tang, F., Martell, S., Shomer, N., Leung, P. C. K., Stephenson, M. D., Rajcan-Separovic, E. Tags: Articles Source Type: research
Statins inhibit blastocyst formation by preventing geranylgeranylation
This study was supported by grants from the George F. Straub Trust of the Hawaii Community Foundation (13ADVC-60315 to V.B.A.) and the National Institutes of Health, USA (P20GM103457 to V.B.A.). The authors have no conflict of interest to declare. (Source: Molecular Human Reproduction)
Source: Molecular Human Reproduction - April 26, 2016 Category: Molecular Biology Authors: Alarcon, V. B., Marikawa, Y. Tags: Articles Source Type: research
Inhibitors of c-Jun phosphorylation impede ovine primordial follicle activation
STUDY HYPOTHESIS
Is the c-Jun-N-terminal kinase (JNK) pathway implicated in primordial follicle activation?
STUDY FINDING
Culture of ovine ovarian cortex in the presence of two different c-Jun phosphorylation inhibitors impeded pre-antral follicle activation.
WHAT IS KNOWN ALREADY
Despite its importance for fertility preservation therapies, the mechanisms of primordial follicle activation are poorly understood. Amongst different signalling pathways potentially involved, the JNK pathway has been previously shown to be essential for cell cycle progression and pre-antral follicle development in mice.
STUDY DESIGN, SAMPLES/...
Source: Molecular Human Reproduction - April 26, 2016 Category: Molecular Biology Authors: Bertoldo, M. J., Bernard, J., Duffard, N., Tsikis, G., Alves, S., Calais, L., Uzbekova, S., Monniaux, D., Mermillod, P., Locatelli, Y. Tags: Articles Source Type: research
(Partial) Loss of BAF250a (ARID1A) in rectovaginal deep-infiltrating endometriosis, endometriomas and involved pelvic sentinel lymph nodes
This study was supported by an internal Charité grant to the Endometriosis Research Center and the authors declare no conflicts of interest. (Source: Molecular Human Reproduction)
Source: Molecular Human Reproduction - April 26, 2016 Category: Molecular Biology Authors: Borrelli, G. M., Abrao, M. S., Taube, E. T., Darb-Esfahani, S., Kohler, C., Chiantera, V., Mechsner, S. Tags: Articles Source Type: research
Germ stem cells are active in postnatal mouse ovary under physiological conditions
This study was conducted in mice. Whether or not the results are applicable to human remain unclear. The future work should aim at identifying the specific ovarian germ stem cell marker and evaluating the significance of these stem cells to normal ovarian function.
WIDER IMPLICATIONS OF THE FINDINGS
Clarifying the existence of active germ stem cells and their functional significance in postnatal mammalian ovaries could provide new insights in understanding the mechanism of ovarian aging and failure.
LARGE SCALE DATA
Not applicable.
STUDY FUNDING/COMPETING INTEREST(S)
This work was supported by the National Key Basic Res...
Source: Molecular Human Reproduction - April 26, 2016 Category: Molecular Biology Authors: Guo, K., Li, C.-h., Wang, X.-y., He, D.-j., Zheng, P. Tags: Articles Source Type: research
Replenishing the adult ovarian follicle population: a fresh look at dogma
(Source: Molecular Human Reproduction)
Source: Molecular Human Reproduction - April 26, 2016 Category: Molecular Biology Authors: Anderson, R. A., Telfer, E. E. Tags: Invited Commentary Source Type: research
The double-edged sword of the mammalian oocyte - advantages, drawbacks and approaches for basic and clinical analysis at the single cell level
(Source: Molecular Human Reproduction)
Source: Molecular Human Reproduction - March 31, 2016 Category: Molecular Biology Authors: Brayboy, L. M., Wessel, G. M. Tags: CORRIGENDUM Source Type: research
Copper metabolism domain-containing 1 represses the mediators involved in the terminal effector pathways of human labour and delivery
STUDY HYPOTHESIS
Does Copper Metabolism MURR1 Domain 1 (COMMD1) play a role in regulating the mediators involved in the terminal processes of human labour and delivery?
STUDY FINDING
COMMD1 plays a critical role in the termination of nuclear factor-B (NF-B) activity and the control of pro-inflammatory and pro-labour mediators.
WHAT IS KNOWN ALREADY
Inflammation and infection are the biggest aetiological factors associated with preterm birth. NF-B drives the transcription of pro-inflammatory mediators involved in the terminal effector pathways of human labour and delivery. In non-gestational tissues, COMMD1 is a negative ...
Source: Molecular Human Reproduction - March 31, 2016 Category: Molecular Biology Authors: Lappas, M. Tags: Articles Source Type: research
Female human pluripotent stem cells rapidly lose X chromosome inactivation marks and progress to a skewed methylation pattern during culture
STUDY HYPOTHESIS
Does a preferential X chromosome inactivation (XCI) pattern exist in female human pluripotent stem cells (hPSCs) and does the pattern change during long-term culture or upon differentiation?
STUDY FINDING
We identified two independent phenomena that lead to aberrant XCI patterns in female hPSC: a rapid loss of histone H3 lysine 27 trimethylation (H3K27me3) and long non-coding X-inactive specific transcript (XIST) expression during culture, often accompanied by erosion of XCI-specific methylation, and a frequent loss of random XCI in the cultures.
WHAT IS KNOWN ALREADY
Variable XCI patterns have been repo...
Source: Molecular Human Reproduction - March 31, 2016 Category: Molecular Biology Authors: Geens, M., Seriola, A., Barbe, L., Santalo, J., Veiga, A., Dee, K., Van Haute, L., Sermon, K., Spits, C. Tags: Articles Source Type: research
The mouse endometrium contains epithelial, endothelial and leucocyte populations expressing the stem cell marker telomerase reverse transcriptase
This study was funded by National Health and Medical Research Council (NHMRC) of Australia grants (1085435, C.E.G., J.A.D.), 1021127 (C.E.G.), NHMRC Senior Research Fellowship (1042298, C.E.G.), the Victorian Infrastructure Support Program, U.S. National Institutes of Health grant R01 DK084056 (D.T.B.) and the Harvard Stem Cell Institute (D.T.B.). The authors have no conflicts of interest to declare. (Source: Molecular Human Reproduction)
Source: Molecular Human Reproduction - March 31, 2016 Category: Molecular Biology Authors: Deane, J. A., Ong, Y. R., Cain, J. E., Jayasekara, W. S. N., Tiwari, A., Carlone, D. L., Watkins, D. N., Breault, D. T., Gargett, C. E. Tags: Articles Source Type: research
Oocyte mitochondrial deletions and heteroplasmy in a bovine model of ageing and ovarian stimulation
STUDY HYPOTHESIS
Maternal ageing and ovarian stimulation result in the accumulation of mitochondrial DNA (mtDNA) deletions and heteroplasmy in individual oocytes from a novel bovine model for human assisted reproductive technology (ART).
STUDY FINDING
The levels of mtDNA deletions detected in oocytes increased with ovarian ageing. Low levels of mtDNA heteroplasmy were apparent across oocytes and no relationship was identified with respect to ovarian ageing or ovarian stimulation.
WHAT IS KNOWN ALREADY
Oocyte quality decreases with ovarian ageing and it is postulated that the mtDNA may have a role in this decline. The imp...
Source: Molecular Human Reproduction - March 31, 2016 Category: Molecular Biology Authors: Hammond, E. R., Green, M. P., Shelling, A. N., Berg, M. C., Peek, J. C., Cree, L. M. Tags: Articles Source Type: research
Decrease in expression of maternal effect gene Mater is associated with maternal ageing in mice
This study was conducted in mice. Whether or not the results are applicable to human remains further elucidation. In addition, we were unable to confirm if the strain of mice (C57BL/6XSv129) at the age of 41–68 weeks old has the ‘cohesin-loss’ phenotype.
WIDER IMPLICATIONS OF THE FINDINGS
Investigating Mater's functional mechanisms could provide fresh insights into understanding how the ageing-related oocyte quality decline occurs.
LARGE SCALE DATA
N/A.
STUDY FUNDING AND COMPETING INTEREST(S)
This work was supported by the research grant from Chinese NSFC to P.Z. (31071274). We have no conflict of inte...
Source: Molecular Human Reproduction - March 31, 2016 Category: Molecular Biology Authors: Lu, Y.-q., He, X.-c., Zheng, P. Tags: Articles Source Type: research
Fertilization defects in sperm from Cysteine-rich secretory protein 2 (Crisp2) knockout mice: implications for fertility disorders
This study was supported by the World Health Organization (H9/TSA/037), the National Research Council of Argentina (PIP 2009-290), the National Agency for Scientific and Technological Promotion of Argentina (PICT 2011, 2023) and the Rene Baron Foundation to P.S.C. and by the MEXT of Japan to M.I. The authors declare that there are no conflicts of interest. (Source: Molecular Human Reproduction)
Source: Molecular Human Reproduction - March 31, 2016 Category: Molecular Biology Authors: Brukman, N. G., Miyata, H., Torres, P., Lombardo, D., Caramelo, J. J., Ikawa, M., Da Ros, V. G., Cuasnicu, P. S. Tags: Articles Source Type: research
