Isolation and gene expression analysis of single potential human spermatogonial stem cells
STUDY HYPOTHESIS
It is possible to isolate pure populations of single potential human spermatogonial stem cells without somatic contamination for down-stream applications, for example cell culture and gene expression analysis.
STUDY FINDING
We isolated pure populations of single potential human spermatogonial stem cells (hSSC) without contaminating somatic cells and analyzed gene expression of these cells via single-cell real-time RT–PCR.
WHAT IS KNOWN ALREADY
The isolation of a pure hSSC fraction could enable clinical applications such as fertility preservation for prepubertal boys and in vitro-spermatogenesis. By...
Source: Molecular Human Reproduction - March 31, 2016 Category: Molecular Biology Authors: von Kopylow, K., Schulze, W., Salzbrunn, A., Spiess, A.- N. Tags: Articles Source Type: research
Thank you to our reviewers
(Source: Molecular Human Reproduction)
Source: Molecular Human Reproduction - February 25, 2016 Category: Molecular Biology Tags: Thank you to our reviewers Source Type: research
Does mouse embryo primordial germ cell activation start before implantation as suggested by single-cell transcriptomics dynamics?
STUDY HYPOTHESIS
Does primordial germ cell (PGC) activation start before mouse embryo implantation, and does the possible regulation of the DNA (cytosine-5-)-methyltransferase 3-like (Dnmt3l) by transcription factor AP-2, gamma (TCFAP2C) have a role in this activation and in the primitive endoderm (PE)–epiblast (EPI) lineage specification?
STUDY FINDING
A burst of expression of PGC markers, such as Dppa3/Stella, Ifitm2/Fragilis, Fkbp6 and Prdm4, is observed from embryonic day (E) 3.25, and some of them, together with the late germ cell markers Zp3, Mcf2 and Morc1, become restricted to the EPI subpopulation at E4.5, ...
Source: Molecular Human Reproduction - February 25, 2016 Category: Molecular Biology Authors: Gerovska, D., Arauzo-Bravo, M. J. Tags: Article Source Type: research
The double-edged sword of the mammalian oocyte - advantages, drawbacks and approaches for basic and clinical analysis at the single cell level
Oocytes are usually the largest cells in the body and as such offer unique opportunities for single-cell analysis. Unfortunately, these cells are also some of the rarest in the mammalian female, usually necessitating single-cell analysis. In cases of infertility in humans, determining the quality of the oocyte is often restricted to a morphological analysis or to the study of cellular behaviors in the developing embryo. Minimally invasive approaches could greatly assist the clinician to prioritize oocytes for fertilization or following fertilization, which embryo to transfer back into the woman. Transcriptomics of human an...
Source: Molecular Human Reproduction - February 25, 2016 Category: Molecular Biology Authors: Brayboy, L. M., Wessel, G. M. Tags: New Research Horizon Reviews Source Type: research
Proteomics of Xenopus development
Modern mass spectrometry-based methods provide an exciting opportunity to characterize protein expression in the developing embryo. We have employed an isotopic labeling technology to quantify the expression dynamics of nearly 6000 proteins across six stages of development in Xenopus laevis from the single stage zygote through the mid-blastula transition and the onset of organogenesis. Approximately 40% of the proteins show significant changes in expression across the development stages. The expression changes for these proteins naturally falls into six clusters corresponding to major events that mark early Xenopus develop...
Source: Molecular Human Reproduction - February 25, 2016 Category: Molecular Biology Authors: Sun, L., Champion, M. M., Huber, P. W., Dovichi, N. J. Tags: New Research Horizon Reviews Source Type: research
