Comprehensive Analysis of the miRNA-mRNA Pathological Regulatory Network of Intestinal CD4 + T Cells in Parkinson’s Disease
AbstractInfiltration of CD4 + T cells was found in brain tissue samples from PD patients, suggesting their involvement in developing central nervous system (CNS) disease. The idea of the gut-brain axis further corroborates intestinal T cells’ activation as the central immune response initiation. However, the specific fa ctors and molecular pathways regulating intestinal T-cell activation are unclear. We used the GSE156287 and GSE145814 datasets from the GEO database to analyze and obtain the miRNAs, which are aberrantly expressed in intestinal CD4 + T cells in PD patients and predict their regulatory target mRN ...
Source: Journal of Molecular Neuroscience - June 24, 2023 Category: Neuroscience Source Type: research
PSRC1 Regulated by DNA Methylation Is a Novel Target for LGG Immunotherapy
In conclusion, this study identified the pathogenic role ofPSRC1 in the pathological progression of LGG, expanding the molecular understanding ofPSRC1, and provided a biomarker and potential immunotherapeutic target for the treatment of LGG. (Source: Journal of Molecular Neuroscience)
Source: Journal of Molecular Neuroscience - June 16, 2023 Category: Neuroscience Source Type: research
Transcriptomic Analyses of Neurotoxic Astrocytes Derived from Adult Triple Transgenic Alzheimer ’s Disease Mice
AbstractNeurodegenerative diseases such as Alzheimer ’s disease have been classically studied from a purely neuronocentric point of view. More recent evidences support the notion that other cell populations are involved in disease progression. In this sense, the possible pathogenic role of glial cells like astrocytes is increasingly being recognized . Once faced with tissue damage signals and other stimuli present in disease environments, astrocytes suffer many morphological and functional changes, a process referred as reactive astrogliosis. Studies from murine models and humans suggest that these complex and heterogene...
Source: Journal of Molecular Neuroscience - June 15, 2023 Category: Neuroscience Source Type: research
An Imbalance in the Pro/mature BDNF Ratio Occurs in Multiple Brain Regions During Normal Ageing in Wild-Type Mice
AbstractThe early transition to Alzheimer ’s disease is characterized by a period of accelerated brain atrophy that exceeds normal ageing. Identifying the molecular basis of this atrophy could facilitate the discovery of novel drug targets. The precursor of brain-derived neurotrophic factor, a well characterized neurotrophin, is increased in the hippocampus of aged rodents, while its mature isoform is relatively stable. This imbalance could increase the risk of Alzheimer’s disease by precipitating its pathological hallmarks. However, less is known about how relative levels of these isoforms change in middle-aged mice. ...
Source: Journal of Molecular Neuroscience - June 14, 2023 Category: Neuroscience Source Type: research
H2S Alleviates Neuropathic Pain in Mice by Nrf2 Signaling Pathway Activation
In this study, we sought to ascertain whether H2S therapy could alleviate neuropathic pain in a model of chronic constriction injury (CCI) and, if so, the potential mechanism. A CCI model was established in mice through a spinal nerve ligation method. Intrathecal injection of NaHS was used to treat CCI model mice. The thermal paw withdrawal latency (TPWL) and mechanical paw withdrawal threshold (MPWT) were used for pain threshold evaluation in mice. A series of experiments including immunofluorescence, enzyme-linked immunosorbent assay, electrophysiological test, mitochondrial DNA (mtDNA) quantification, measurement of ATP...
Source: Journal of Molecular Neuroscience - June 9, 2023 Category: Neuroscience Source Type: research
Association of Telomere Length with Cognitive Impairment
AbstractTelomere attrition is attributed to Alzheimer ’s disease (AD), major depressive disorder, stress levels, physical inactivity, short sleep duration, and reduced educational abilities. In this article, we tried to assess the association between the telomere length in peripheral blood leukocytes and level of cognitive impairment and its dependen ce on age and sex. Healthy subjects and patients with amnestic mild cognitive impairment (aMCI) and different AD stages were recruited in the study. All patients were assessed by the same standard diagnostic procedure, including neurological examination—Mini-Mental State ...
Source: Journal of Molecular Neuroscience - June 6, 2023 Category: Neuroscience Source Type: research
Transcriptome Profiling of Hippocampus After Cerebral Hypoperfusion in Mice
AbstractChronic cerebral hypoperfusion (CCH) is considered to be one of the major mechanism in the pathogenesis of vascular cognitive impairment (VCI). Increased inflammatory cells, particularly microglia, often parallel hypoperfusion-induced gray matter damage such as hippocampal lesions, but the exact mechanism remains largely unknown. To understand the pathological mechanisms, we analyzed hippocampus-specific transcriptome profiles after cerebral hypoperfusion. The mouse hypoperfusion model was induced by employing the 0.16/0.18 mm bilateral common carotid artery stenosis (BCAS) procedure. Cerebral blood flow (CBF) wa...
Source: Journal of Molecular Neuroscience - June 2, 2023 Category: Neuroscience Source Type: research
RNA Sequencing of Intraoperative Peritumoral Tissues Reveals Potential Pathways Involved in Glioma-Related Seizures
This study aimed to investigate the molecular mechanisms potentially involved in peritumoral epilepsy in low-grade gliomas (LGGs). Intraoperative peritumoral brain tissues resected from LGG patients with seizures (pGRS) or without seizures (pGNS) were used for RNA sequencing (RNA-seq). Comparative transcriptomics was performed to identify differentially expressed genes (DEGs) in pGRS compared to pGNS using deseq2 and edgeR packages (R). Gene set enrichment analysis (GSEA) using Gene Ontology terms and Kyoto Encyclopedia of Genes& Genomes (KEGG) pathways was performed using the clusterProfiler package (R). The expressio...
Source: Journal of Molecular Neuroscience - June 2, 2023 Category: Neuroscience Source Type: research
