Data-Independent Acquisition-Based Serum Proteomic Profiling of Adult Moyamoya Disease Patients Reveals the Potential Pathogenesis of Vascular Changes
AbstractMoyamoya disease (MMD) is a chronic cerebrovascular disease with unknown etiology. The pathogenesis of vascular changes remains unclear. Ischemic and hemorrhagic adult MMD patients and healthy volunteers were enrolled to collect serum for data-independent acquisition (DIA)-based proteomic analysis and ELISA validation. DIA serum proteomic revealed that apolipoprotein C-I (APOC1), apolipoprotein D (APOD), and apolipoprotein A-IV (APOA4) were decreased. The reductases glutathione S-transferase omega-1 (GSTO1) and peptidyl-prolyl cis –trans isomerase A (PPIA) were upregulated, and ADAMTS-like protein 4 (ADAMTSL4) wa...
Source: Journal of Molecular Neuroscience - December 15, 2022 Category: Neuroscience Source Type: research
Whole-Exome Sequencing Study of Consanguineous Parkinson ’s Disease Families and Related Phenotypes: Report of Twelve Novel Variants
AbstractParkinson ’s disease (PD) is a common progressive neurodegenerative disorder with motor and nonmotor symptoms. Recent studies demonstrate various susceptibility loci and candidate genes for familial forms of the disease. However, the genetic basis of the familial form of early-onset PD (EOPD) is not widely studied in the Iranian population. Therefore, the present study aimed to investigate the possible causative genetic variants responsible for developing EOPD among Iranian patients. Iranian patients with a clinical diagnosis of Parkinson’s disease were evaluated, and 12 consanguineous families with at least tw...
Source: Journal of Molecular Neuroscience - December 15, 2022 Category: Neuroscience Source Type: research
Honokiol Reduces Mitochondrial Dysfunction and Inhibits Apoptosis of Nerve Cells in Rats with Traumatic Brain Injury by Activating the Mitochondrial Unfolded Protein Response
This study was designed to determine the effects and underlying mechanism of honokiol (HNK) on traumatic brain injury (TBI). A rat TBI model was constructed using the modified Feeney free-fall percussion method and treatment with HNK via intraperitoneal injection. The brain tissues of the rats in each group were assessed using the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay to detect the level of neuronal apoptosis. Western blots were used to detect the expression levels of apoptosis-related proteins (Bcl-2 and Bax), and ELISAs were used to measure the levels of pro-inflammatory cytokines (IL...
Source: Journal of Molecular Neuroscience - December 12, 2022 Category: Neuroscience Source Type: research
Profiling and Bioinformatics Analyses of Differential Circular RNA Expression in Glioblastoma Multiforme Cells Under Hypoxia
AbstractThe hypoxia microenvironment is highly associated with GBM ’s malignant phenotypes. CircRNAs were reported involved in GBM’s biological characteristics and regulated by HIF-1α. However, the differential expression profile and role of circRNAs in GBM cells under hypoxia are still unclear. The expression profiles of circRNAs in LN229 and T98G under hypox ia were explored via circRNA sequencing analysis. Those circRNAs significantly dysregulated both in LN229 and T98G and could be found in circBase were selected and validated by qRT-PCR, RNase R digestion reaction, and Sanger sequencing. Normal cell line and fres...
Source: Journal of Molecular Neuroscience - December 9, 2022 Category: Neuroscience Source Type: research
Effects of Sevoflurane Exposure on Fetal Brain Development Using Cerebral Organoids
AbstractSevoflurane is a safe and well-known inhaled anesthetic. Given that sevoflurane can be delivered to developing fetuses through the mother, it is critical to determine whether this agent affects fetal neurodevelopment. Recent research has sought to determine whether sevoflurane affects fetal brain development when the mother is exposed during the second to third trimester of pregnancy, considered to be the crucial period for the development of nervous system. However, even though the first trimester is a critical period for fetal organogenesis and the most susceptible time to teratogen exposure, research regarding t...
Source: Journal of Molecular Neuroscience - December 7, 2022 Category: Neuroscience Source Type: research
Lymphotoxin- α Orchestrate Hypoxia and Immune factors to Induce Experimental Cerebral Malaria: Inhibition Mitigates Pathogenesis, Neurodegeneration, and Increase Survival
AbstractKnockdown studies have shown lymphotoxin- α (Lt-α) as a critical molecule for Experimental cerebral malaria (ECM) pathogenesis. We investigated the role of lymphotoxin-α in regulating active caspase-3 and calpain1. T cell infiltration into the brains, and subsequent neuronal cell death are the essential features ofPlasmodium berghei ANKA(PbA)-induced ECM. Our results showed increased Lt- α levels during ECM. Treatment of naïve mice with serum from ECM mice and exogenous Lt-α was lethal. We inhibited Lt-α in vivo duringPbA infection by injecting the mice with anti-Lt- α antibody. Inhibition of Lt-α mitigate...
Source: Journal of Molecular Neuroscience - December 5, 2022 Category: Neuroscience Source Type: research
Exploring the Correlation of Abnormal CXCL9 Expression with Immune Infiltration in Glioma and Patient Prognosis Based on TCGA and GTEx Databases
In this study, we intend to identify key immune-related genes (IRGs) in gliomas using the TCGA and GTEx databases. Following collection of the RNA-seq data of lower-grade glioma (LGG) and glioblastoma (GBM) patients from the TCGA and GTEx databases, the differentially expressed IRGs (DE-IRGs) were screened. The ESTIMATE algorithm was utilized to evaluate StromalScore and ImmuneScore of LGG and GBM samples and a multifactorial Cox risk model was constructed to identify the related risk genes. The core IRGs of LGG and GBM were screened through a PPI network, followed by exploration of their correlation with glioma prognosis....
Source: Journal of Molecular Neuroscience - November 30, 2022 Category: Neuroscience Source Type: research
Identification of Key Genes Associated with Risk and Prognosis of Neuroblastoma
This study identified 5 key genes for neuroblastoma risk and prognosis that were potential biomarkers. (Source: Journal of Molecular Neuroscience)
Source: Journal of Molecular Neuroscience - November 29, 2022 Category: Neuroscience Source Type: research
