Understanding the effects of mesenchymal stromal cell therapy for treating osteoarthritis using an in vitro co-culture model
Eur Cell Mater. 2023 Jun 19;45:143-157. doi: 10.22203/eCM.v045a10.ABSTRACTOsteoarthritis (OA) is a leading cause of chronic pain and disability, for which there is no cure. Mesenchymal stromal cells (MSCs) have been used in clinical trials for treating OA due to their unique ability to generate paracrine anti-inflammatory and trophic signals. Interestingly, these studies have shown mainly short-term effects of MSCs in improving pain and joint function, rather than sustained and consistent benefits. This may reflect a change or loss in the therapeutic effects of MSCs after intra-articular injection. The present study aimed ...
Source: European Cells and Materials - June 19, 2023 Category: Cytology Authors: V Shang J Li C B Little J J Li Source Type: research
Disrupting mechanical homeostasis promotes matrix metalloproteinase-13 mediated processing of neuron glial antigen 2 in mandibular condylar cartilage
This study focused on MMP-13 mediated changes in a transmembrane proteoglycan, Neuron Glial antigen 2 (NG2/CSPG4). NG2/CSPG4 is a receptor for type VI collagen and a known substrate for MMP-13. In healthy articular layer chondrocytes, NG2/CSPG4 is membrane bound but becomes internalised during TMJ OA. The objective of this study was to determine if MMP-13 contributed to the cleavage and internalisation of NG2/CSPG4 during mechanical loading and OA progression. Using preclinical and clinical samples, it was shown that MMP-13 was present in a spatiotemporally consistent pattern with NG2/CSPG4 internalisation during TMJ OA. I...
Source: European Cells and Materials - May 8, 2023 Category: Cytology Authors: M Bagheri Varzaneh Y Zhao J Rozynek M Han D A Reed Source Type: research
