Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 h Postinfection (hpi). Ultrastructural an...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research
Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 h Postinfection (hpi). Ultrastructural an...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research
Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 h Postinfection (hpi). Ultrastructural an...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research
Rickettsia rickettsii and Rickettsia typhi in hospitalized children diagnosed with Pediatric Multisystemic Inflammatory Syndrome in Merida, Yucatan
In conclusion MIS-C should be considered in children with elevated inflammatory markers when there is a history of COVID-19 and they also meet criteria that have already been established by international agencies, such as CDC and WHO.PMID:38070540 | DOI:10.1016/j.bjid.2023.103707 (Source: Braz J Infect Dis)
Source: Braz J Infect Dis - December 9, 2023 Category: Infectious Diseases Authors: Karla Dzul-Rosado Dayana Guadalupe Lav ín-Sánchez Gerardo Álvarez Hernández Salvador G ómez-Carro Fernando I Puerto-Manzano Source Type: research
Rickettsia rickettsii and Rickettsia typhi in hospitalized children diagnosed with Pediatric Multisystemic Inflammatory Syndrome in Merida, Yucatan
In conclusion MIS-C should be considered in children with elevated inflammatory markers when there is a history of COVID-19 and they also meet criteria that have already been established by international agencies, such as CDC and WHO.PMID:38070540 | DOI:10.1016/j.bjid.2023.103707 (Source: Braz J Infect Dis)
Source: Braz J Infect Dis - December 9, 2023 Category: Infectious Diseases Authors: Karla Dzul-Rosado Dayana Guadalupe Lav ín-Sánchez Gerardo Álvarez Hernández Salvador G ómez-Carro Fernando I Puerto-Manzano Source Type: research
The impact of COVID-19 on microbiological profile and antibiotic consumption in ICU: a retrospective study in an infectious disease hospital in Brazil
CONCLUSION: The shifting microbiological profile can be attributed to both the unique characteristics of patients with COVID-19 and the adjustments made to healthcare facilities' structural and work routines. Understanding these changes is essential in addressing the accelerated impact of antimicrobial resistance during the pandemic. Therefore, conducting thorough reviews of institutional practices and routines becomes critical in mitigating the consequences of antimicrobial resistance and its implications for patient care.PMID:38065221 | DOI:10.1016/j.bjid.2023.103705 (Source: Braz J Infect Dis)
Source: Braz J Infect Dis - December 8, 2023 Category: Infectious Diseases Authors: Ver ônica de França Diniz Rocha Euclimeire Neves da Silva Jailton Azevedo Marcelo Teles Ribeiro Mitermayer Galv ão Reis Tania Fraga Barros Joice Neves Reis Source Type: research
Comparative analysis of Respiratory Syncytial Virus frequency rates and viral load in different patient cohorts in a University Hospital in S ão Paulo, Brazil, over an eight-year period (2005-2013)
In conclusion, high RSV viral load among hospitalized patients could serve as a surrogate marker of disease severity. Additionally, patients with chronic heart disease deserve greater attention regarding complications associated with RSV infection.PMID:38043582 | DOI:10.1016/j.bjid.2023.103702 (Source: Braz J Infect Dis)
Source: Braz J Infect Dis - December 3, 2023 Category: Infectious Diseases Authors: Luciano Kleber de Souza Luna Jessica Santiago Cruz T ânia do Socorro Souza Chaves Nancy Bellei Source Type: research
Comparative analysis of Respiratory Syncytial Virus frequency rates and viral load in different patient cohorts in a University Hospital in S ão Paulo, Brazil, over an eight-year period (2005-2013)
In conclusion, high RSV viral load among hospitalized patients could serve as a surrogate marker of disease severity. Additionally, patients with chronic heart disease deserve greater attention regarding complications associated with RSV infection.PMID:38043582 | DOI:10.1016/j.bjid.2023.103702 (Source: Braz J Infect Dis)
Source: Braz J Infect Dis - December 3, 2023 Category: Infectious Diseases Authors: Luciano Kleber de Souza Luna Jessica Santiago Cruz T ânia do Socorro Souza Chaves Nancy Bellei Source Type: research
Comparative analysis of Respiratory Syncytial Virus frequency rates and viral load in different patient cohorts in a University Hospital in S ão Paulo, Brazil, over an eight-year period (2005-2013)
In conclusion, high RSV viral load among hospitalized patients could serve as a surrogate marker of disease severity. Additionally, patients with chronic heart disease deserve greater attention regarding complications associated with RSV infection.PMID:38043582 | DOI:10.1016/j.bjid.2023.103702 (Source: Braz J Infect Dis)
Source: Braz J Infect Dis - December 3, 2023 Category: Infectious Diseases Authors: Luciano Kleber de Souza Luna Jessica Santiago Cruz T ânia do Socorro Souza Chaves Nancy Bellei Source Type: research
Comparative analysis of Respiratory Syncytial Virus frequency rates and viral load in different patient cohorts in a University Hospital in S ão Paulo, Brazil, over an eight-year period (2005-2013)
In conclusion, high RSV viral load among hospitalized patients could serve as a surrogate marker of disease severity. Additionally, patients with chronic heart disease deserve greater attention regarding complications associated with RSV infection.PMID:38043582 | DOI:10.1016/j.bjid.2023.103702 (Source: Braz J Infect Dis)
Source: Braz J Infect Dis - December 3, 2023 Category: Infectious Diseases Authors: Luciano Kleber de Souza Luna Jessica Santiago Cruz T ânia do Socorro Souza Chaves Nancy Bellei Source Type: research
Comparative analysis of Respiratory Syncytial Virus frequency rates and viral load in different patient cohorts in a University Hospital in S ão Paulo, Brazil, over an eight-year period (2005-2013)
In conclusion, high RSV viral load among hospitalized patients could serve as a surrogate marker of disease severity. Additionally, patients with chronic heart disease deserve greater attention regarding complications associated with RSV infection.PMID:38043582 | DOI:10.1016/j.bjid.2023.103702 (Source: Braz J Infect Dis)
Source: Braz J Infect Dis - December 3, 2023 Category: Infectious Diseases Authors: Luciano Kleber de Souza Luna Jessica Santiago Cruz T ânia do Socorro Souza Chaves Nancy Bellei Source Type: research
Clinical study of hepatitis B vaccine in achieving hepatitis B surface antibody seroconversion in patients with functional cure
Braz J Infect Dis. 2023 Nov 27:103703. doi: 10.1016/j.bjid.2023.103703. Online ahead of print.ABSTRACTHepatitis B Surface Antigen (HBsAg) seroclearance is the highest treatment goal recommended by the current guidelines for hepatitis B. Levels of antibodies to HBsAg (anti-HBs) are strongly associated with HBsAg recurrence, but hepatitis B vaccination may increase the anti-HBs seroconversion rate and reduce recurrence. We conducted a retrospective clinical study to ascertain the effect of this vaccination on the seroconversion rate and levels of protective anti-HBs after HBsAg. In this retrospective study, we distributed a ...
Source: Braz J Infect Dis - November 30, 2023 Category: Infectious Diseases Authors: Yisi Liu Shan Ren Lina Ma Xiao Lin Hong Li Junfeng Lu Zhenhuan Cao Sujun Zheng Zhongjie Hu Xinyue Chen Source Type: research
Seroprevalence of viral transfusion transmissible infections (HBsAg, anti-HCV, anti-HIV, Syphilis) and coinfection among healthy volunteer blood donors during 5-years in Luanda, Angola
CONCLUSION: A high transfusion-transmissible infection prevalence was found compared to some countries in Sub-Saharan Africa. Therefore, intensifying the screening for these transfusion-transmitted infections in blood donors is critical to ensure blood safety.PMID:38036021 | DOI:10.1016/j.bjid.2023.103704 (Source: Braz J Infect Dis)
Source: Braz J Infect Dis - November 30, 2023 Category: Infectious Diseases Authors: Angelina Edna Quintas Cl áudia Camila Dias Adis Del Carmen Cogle Lemuel Cordeiro Ant ónio Sarmento Source Type: research
Clinical study of hepatitis B vaccine in achieving hepatitis B surface antibody seroconversion in patients with functional cure
Braz J Infect Dis. 2023 Nov 27:103703. doi: 10.1016/j.bjid.2023.103703. Online ahead of print.ABSTRACTHepatitis B Surface Antigen (HBsAg) seroclearance is the highest treatment goal recommended by the current guidelines for hepatitis B. Levels of antibodies to HBsAg (anti-HBs) are strongly associated with HBsAg recurrence, but hepatitis B vaccination may increase the anti-HBs seroconversion rate and reduce recurrence. We conducted a retrospective clinical study to ascertain the effect of this vaccination on the seroconversion rate and levels of protective anti-HBs after HBsAg. In this retrospective study, we distributed a ...
Source: Braz J Infect Dis - November 30, 2023 Category: Infectious Diseases Authors: Yisi Liu Shan Ren Lina Ma Xiao Lin Hong Li Junfeng Lu Zhenhuan Cao Sujun Zheng Zhongjie Hu Xinyue Chen Source Type: research
Seroprevalence of viral transfusion transmissible infections (HBsAg, anti-HCV, anti-HIV, Syphilis) and coinfection among healthy volunteer blood donors during 5-years in Luanda, Angola
CONCLUSION: A high transfusion-transmissible infection prevalence was found compared to some countries in Sub-Saharan Africa. Therefore, intensifying the screening for these transfusion-transmitted infections in blood donors is critical to ensure blood safety.PMID:38036021 | DOI:10.1016/j.bjid.2023.103704 (Source: Braz J Infect Dis)
Source: Braz J Infect Dis - November 30, 2023 Category: Infectious Diseases Authors: Angelina Edna Quintas Cl áudia Camila Dias Adis Del Carmen Cogle Lemuel Cordeiro Ant ónio Sarmento Source Type: research
