Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 hours postinfection (hpi). Ultrastructura...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research

Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 hours postinfection (hpi). Ultrastructura...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research

Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 hours postinfection (hpi). Ultrastructura...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research

Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 hours postinfection (hpi). Ultrastructura...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research

Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 hours postinfection (hpi). Ultrastructura...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research

Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 hours postinfection (hpi). Ultrastructura...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research

Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 hours postinfection (hpi). Ultrastructura...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research

Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 hours postinfection (hpi). Ultrastructura...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research

Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 hours postinfection (hpi). Ultrastructura...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research

Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 hours postinfection (hpi). Ultrastructura...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research

Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 hours postinfection (hpi). Ultrastructura...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research

Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 h Postinfection (hpi). Ultrastructural an...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research

Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 h Postinfection (hpi). Ultrastructural an...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research

Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 h Postinfection (hpi). Ultrastructural an...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research

Comparative analysis of SARS-CoV-2 variants Alpha (B.1.1.7), Gamma (P.1), Zeta (P.2) and Delta (B.1.617.2) in Vero-E6 cells: ultrastructural characterization of cytopathology and replication kinetics
This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 h Postinfection (hpi). Ultrastructural an...
Source: Braz J Infect Dis - December 11, 2023 Category: Infectious Diseases Authors: Debora Ferreira Barreto Vieira Derick Mendes Bandeira Marcos Alexandre Nunes da Silva Ana Luisa Teixeira de Almeida Mia Ara újo Ana Beatriz Machado Luis Fernando Lopez Tort Val éria Pereira Nacife Marilda M Siqueira Fernando Couto Motta Alex Pauvolid-Co Source Type: research